1,151 research outputs found
Conditional expression in corticothalamic efferents reveals a developmental role for nicotinic acetylcholine receptors in modulation of passive avoidance behavior
Prenatal nicotine exposure has been linked to attention deficit hyperactivity disorder and cognitive impairment, but the sites of action for these effects of nicotine are still under investigation. High-affinity nicotinic acetylcholine receptors (nAChRs) contain the .2 subunit and modulate passive avoidance (PA) learning in mice. Using an inducible, tetracycline-regulated transgenic system, we generated lines of mice with expression of high-affinity nicotinic receptors restored in specific neuronal populations. One line of mice shows functional .2 subunit-containing nAChRs localized exclusively in corticothalamic efferents. Functional, presynaptic nAChRs are present in the thalamus of these mice as detected by nicotine-elicited rubidium efflux assays from synaptosomes. Knock-out mice lacking high-affinity nAChRs show elevated baseline PA learning, whereas normal baseline PA behavior is restored in mice with corticothalamic expression of these nAChRs. In contrast, nicotine can enhance PA learning in adult wild-type animals but not in corticothalamic-expressing transgenic mice. When these transgenic mice are treated with doxycycline in adulthood to switch off nAChR expression, baseline PA is maintained even after transgene expression is abolished. These data suggest that high-affinity nAChRs expressed on corticothalamic neurons during development are critical for baseline PA performance and provide a potential neuroanatomical substrate for changes induced by prenatal nicotine exposure leading to long-term behavioral and cognitive deficits
High-Contrast NIR Polarization Imaging of MWC480
One of the key predictions of modeling from the IR excess of Herbig Ae stars
is that for protoplanetary disks, where significant grain growth and settling
has occurred, the dust disk has flattened to the point that it can be partially
or largely shadowed by the innermost material at or near the dust sublimation
radius. When the self-shadowing has already started, the outer disk is expected
to be detected in scattered light only in the exceptional cases that the scale
height of the dust disk at the sublimation radius is smaller than usual.
High-contrast imaging combined with the IR spectral energy distribution allow
us to measure the degree of flattening of the disk, as well as to determine the
properties of the outer disk. We present polarimetric differential imaging in
band obtained with Subaru/HiCIAO of one such system, MWC 480. The HiCIAO
data were obtained at a historic minimum of the NIR excess. The disk is
detected in scattered light from 0\farcs2-1\farcs0 (27.4-137AU). Together with
the marginal detection of the disk from 1998 February 24 by HST/NICMOS, our
data constrain the opening half angle for the disk to lie between
1.3. When compared with similar measures in CO for
the gas disk from the literature, the dust disk subtends only 30% of the
gas disk scale height (H/R0.03). Such a dust disk is a factor of 5-7
flatter than transitional disks, which have structural signatures that giant
planets have formed.Comment: 21 pages, 6 figures, 1 table, ApJ accepted 2012-05-0
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Observations of Past Lunar Landing Sites by the D-CIXS X-Ray Spectrometer on SMART-1
D-CIXS initial observations show a first unambiguous remote sensing of calcium in the lunar regolith. Data obtained are broadly consistent with current understanding of mare and highland composition. Ground truth is provided by the returned Apollo and Luna sample sets
Iterative graph cuts for image segmentation with a nonlinear statistical shape prior
Shape-based regularization has proven to be a useful method for delineating
objects within noisy images where one has prior knowledge of the shape of the
targeted object. When a collection of possible shapes is available, the
specification of a shape prior using kernel density estimation is a natural
technique. Unfortunately, energy functionals arising from kernel density
estimation are of a form that makes them impossible to directly minimize using
efficient optimization algorithms such as graph cuts. Our main contribution is
to show how one may recast the energy functional into a form that is
minimizable iteratively and efficiently using graph cuts.Comment: Revision submitted to JMIV (02/24/13
Dopamine D_2-receptor activation elicits akinesia, rigidity, catalepsy, and tremor in mice expressing hypersensitive 4 nicotinic receptors via a cholinergic-dependent mechanism
Recent studies suggest that high-affinity neuronal nicotinic acetylcholine receptors (nAChRs) containing α4 and β2 subunits (α4β2*) functionally interact with G-protein-coupled dopamine (DA) D_2 receptors in basal ganglia. We hypothesized that if a functional interaction between these receptors exists, then mice expressing an M2 point mutation (Leu9'Ala) rendering 4 nAChRs hypersensitive to ACh may exhibit altered sensitivity to a D_2-receptor agonist. When challenged with the D_(2)R agonist, quinpirole (0.5–10 mg/kg), Leu9'Ala mice, but not wild-type (WT) littermates, developed severe, reversible motor impairment characterized by rigidity, catalepsy, akinesia, and tremor. While striatal DA tissue content, baseline release, and quinpirole-induced DA depletion did not differ between Leu9'Ala and WT mice, quinpirole dramatically increased activity of cholinergic striatal interneurons only in mutant animals, as measured by increased c-Fos expression in choline acetyltransferase (ChAT)-positive interneurons. Highlighting the importance of the cholinergic system in this mouse model, inhibiting the effects of ACh by blocking muscarinic receptors, or by selectively activating hypersensitive nAChRs with nicotine, rescued motor symptoms. This novel mouse model mimics the imbalance between striatal DA/ACh function associated with severe motor impairment in disorders such as Parkinson’s disease, and the data suggest that a D_(2)R–α4*-nAChR functional interaction regulates cholinergic interneuron activity.—Zhao-Shea, R., Cohen, B. N., Just, H., McClure-Begley, T., Whiteaker, P., Grady, S. R., Salminen, O., Gardner, P. D., Lester, H. A., Tapper, A. R. Dopamine D2-receptor activation elicits akinesia, rigidity, catalepsy, and tremor in mice expressing hypersensitive α4 nicotinic receptors via a cholinergic-dependent mechanism
The origin of hydrogen line emission for five Herbig Ae/Be stars spatially resolved by VLTI/AMBER spectro-interferometry
To trace the accretion and outflow processes around YSOs, diagnostic spectral
lines such as the BrG 2.166 micron line are widely used, although due to a lack
of spatial resolution, the origin of the line emission is still unclear.
Employing the AU-scale spatial resolution which can be achieved with infrared
long-baseline interferometry, we aim to distinguish between theoretical models
which associate the BrG line emission with mass infall or mass outflow
processes. Using the VLTI/AMBER instrument, we spatially and spectrally
(R=1500) resolved the inner environment of five Herbig Ae/Be stars (HD163296,
HD104237, HD98922, MWC297, V921Sco) in the BrG emission line as well as in the
adjacent continuum. All objects (except MWC297) show an increase of visibility
within the BrG emission line, indicating that the BrG-emitting region in these
objects is more compact than the dust sublimation radius. For HD98922, our
quantitative analysis reveals that the line-emitting region is compact enough
to be consistent with the magnetospheric accretion scenario. For HD163296,
HD104237, MWC297, and V921Sco we identify a stellar wind or a disk wind as the
most likely line-emitting mechanism. We search for general trends and find that
the size of the BrG-emitting region does not seem to depend on the basic
stellar parameters, but correlates with the H-alpha line profile shape. We find
evidence for at least two distinct BrG line-formation mechanisms. Stars with a
P-Cygni H-alpha line profile and a high mass-accretion rate seem to show
particularly compact BrG-emitting regions (R_BrG/R_cont<0.2), while stars with
a double-peaked or single-peaked H-alpha-line profile show a significantly more
extended BrG-emitting region (0.6<R_BrG/R_cont<1.4), possibly tracing a stellar
wind or a disk wind.Comment: 20 pages; 11 figures; Accepted by A&A; a high quality version of the
paper can be obtained at
http://www.skraus.eu/papers/kraus.HAeBe-BrGsurvey.pd
Integrated microfluidic tmRNA purification and real-time NASBA device for molecular diagnostics.
We demonstrate the first integrated microfluidic tmRNA purification and nucleic acid sequence-based amplification (NASBA) device incorporating real-time detection. The real-time amplification and detection step produces pathogen-specific response in < 3 min from the chip-purified RNA from 100 lysed bacteria. On-chip RNA purification uses a new silica bead immobilization method. On-chip amplification uses custom-designed high-selectivity primers and real-time detection uses molecular beacon fluorescent probe technology; both are integrated on-chip with NASBA. Present in all bacteria, tmRNA (10Sa RNA) includes organism-specific identification sequences, exhibits unusually high stability relative to mRNA, and has high copy number per organism; the latter two factors improve the limit of detection, accelerate time-to-positive response, and suit this approach ideally to the detection of small numbers of bacteria. Device efficacy was demonstrated by integrated on-chip purification, amplification, and real-time detection of 100 E. coli bacteria in 100 microL of crude lysate in under 30 min for the entire process
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