Seasonal variation of inter-limb jumping asymmetries in youth team-sport athletes

The main objective of the present study was to provide seasonal variation data for inter-limb asymmetry in youth elite team-sport athletes. Fifty-nine players performed the single leg countermovement jump (SLCMJ) and the one leg hop for distance (OLHT) tests during pre-season, mid-season and end-season. A repeated-measures analysis of variance was conducted to determine magnitude differences in asymmetry scores between time points. Kappa coefficients (κ) were calculated to determine the levels of agreement for the direction of asymmetry. When comparing inter-limb asymmetry magnitudes across the season, the SLCMJ test showed significantly higher asymmetries at mid-season in comparison with pre-season and end-season (p<0.01, d=-1.03 for pre to mid; p<0.01, d=1.12 for pre to end). However, OLHT inter-limb asymmetry magnitude remained consistent throughout the season (ES range=-0.02 to -0.06). For the direction of asymmetry, levels of agreement ranged from poor to slight in the SLCMJ (k-0.10 to 0.18) and in the OLHT (k-0.21 to 0.18). No significant differences were found between mean asymmetry values at any time point or for either test when comparing males and females. In conclusion, jump height asymmetry during the SLCMJ was the only metric to show significant magnitude changes across the season

Assessing the magnitude and direction of asymmetry in unilateral jump and change of direction speed tasks in youth female team-sport athletes

The direction of inter-limb asymmetries and the change of direction (COD) deficit are two aspects that have increased in recent years. The main objective of the present study was to assess the magnitude of neuromuscular asymmetries in an elite youth female team-sports sample and determine its directionality. Secondary objectives were to evaluate the relationship between COD deficit, linear speed and COD time performance. Elite female youth basketball and handball players (n = 33, age = 16 ± 1.17 y) performed the Single Leg Countermovement Jump in vertical (SLCJ-V), horizontal (SLCJ-H), and lateral (SLCJ-L) directions, the COD and the 10-m sprint. Results showed statistical differences between limbs in all the neuromuscular tests (p &lt; 0.001). The Kappa coefficient showed poor to fair levels of agreement between tasks (K range = -0.087 to 0.233), indicating that asymmetries rarely favoured the same limb between skills. Additionally, small and non-significant correlations were found between the linear sprint capacity and the COD ability. The findings of the present study highlight the independent directionality of asymmetries across tests. The COD deficit does not appear to be much more advantageous than COD total time to measure asymmetry. Practitioners are encouraged to use a fitness testing battery to detect existing side differences and each ability should be specifically trained with functional tasks

Long-term safety and efficacy of trifarotene 50 μg/g cream, a first-in-class RAR-γ selective topical retinoid, in patients with moderate facial and truncal acne.

BackgroundTreatment for both facial and truncal acne has not sufficiently been studied.ObjectivesTo evaluate the long-term safety and efficacy of trifarotene in both facial and truncal acne.MethodsIn a multicentre, open-label, 52-week study, patients with moderate facial and truncal acne received trifarotene 50 μg/g cream (trifarotene). Assessments included local tolerability, safety, investigator and physician's global assessments (IGA, PGA) and quality of life (QOL). A validated QOL questionnaire was completed by the patient at Baseline, Week 12, 26 and 52/ET.ResultsOf 453 patients enrolled, 342 (75.5%) completed the study. Trifarotene-related treatment-emergent adverse events (TEAEs) were reported in 12.6% of patients, and none was serious. Most related TEAEs were cutaneous and occurred during the first 3 months. Signs and symptoms of local tolerability were mostly mild or moderate and severe signs, and symptoms were reported for 2.2% to 7.1% of patients for the face and 2.5% to 5.4% for the trunk. Local irritation increased during the first week of treatment on the face and up to Weeks 2 to 4 on the trunk with both decreasing thereafter. At Week 12, IGA and PGA success rates were 26.6% and 38.6%, respectively. Success rates increased to 65.1% and 66.9%, respectively at Week 52. Overall success (both IGA and PGA success in the same patient) was 57.9% at Week 52. At Week 52 visit, 92/171 (53.8%) patients who had completed their assessments had scores from 0 to 1 (i.e. no effect of acne on their QOL) vs. 47/208 (22.6%) patients at Baseline visit.ConclusionIn this 52-week study, trifarotene was safe, well tolerated and effective in moderate facial and truncal acne

Ring electrode for radio-frequency heating of the cornea: modelling and in vitro experiments

[EN] Radio-frequency thermokeratoplasty (RF-TKP) is a technique used to reshape the cornea curvature by means of thermal lesions using radio-frequency currents. This curvature change allows refractive disorders such as hyperopia to be corrected. A new electrode with ring geometry is proposed for RF-TKP. It was designed to create a single thermal lesion with a full-circle shape. Finite element models were developed, and the temperature distributions in the cornea were analysed for different ring electrode characteristics. The computer results indicated that the maximum temperature in the cornea was located in the vicinity of the ring electrode outer perimeter, and that the lesions had a semi-torus shape. The results also indicated that the electrode thickness, electrode radius and electrode thermal conductivity had a significant influence on the temperature distributions. In addition, in vitro experiments were performed on rabbit eyes. At 5 IN power the lesions were fully circular. Some lesions showed non-uniform characteristics along their circular path. Lesion depth depended on heating duration (60% of corneal thickness for 20s, and 30% for 10s). The results suggest that the critical shrinkage temperature (55-63degreesC) was reached at the central stroma and along the entire circular path in all the cases.Berjano, E.; Saiz Rodríguez, FJ.; Alió, J.; Ferrero, JM. (2003). Ring electrode for radio-frequency heating of the cornea: modelling and in vitro experiments. Medical & Biological Engineering & Computing. 41(6):630-639. https://doi.org/10.1007/BF02349970S630639416Alió, J. L., Ismail, M. M., Artola, A., andPérez-Santonja, J. J. (1997a): ‘Correction of hyperopia induced by photorefractive keratectomy using non-contact Ho: YAG laser thermal keratoplasty’,J. Refract. Surg.,13, pp. 13–16Alió, J. L., Ismail, M. M., andSanchez, J. L. (1997b): ‘Correction of hyperopia with non-contact Ho: YAG laser thermal keratoplasty’,J. Refract. Surg.,13, pp. 17–22Alió, J. 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Evolución de la agilidad y velocidad lineal en una muestra de adolescentes durante los últimos 20 años (1998-2018)

The aim of the present study was to analyse the changes in the physical capacities of agility and linear velocity in a sample of Spanish schoolchildren during a period of 20 years (1998-2018). For this purpose, a cross-sectional study was designed and the results of the 10x10 shuttle test and 45-meter dash test were analysed. The sample was configured by a total of 3056 schoolchildren between 12 and 13 years old (1792 males and 1264 females). The results showed a decrease in test performance in both sexes for the 10x10 shuttle test. On the contrary, no significant changes were obtained in relation to the performance of the test of the 45-meter dash. The increase in the sedentary lifestyles of industrialized societies, the increase in the obesity proportion, the abuse of new technologies or new social habits could explain the worsening of agility in the study sample.El objetivo del presente estudio fue analizar los cambios en las capacidades físicas de la agilidad y la velocidad lineal en una muestra de escolares españoles durante un período de 20 años (1998-2018). Para este propósito, se diseñó un estudio transversal y se analizaron los resultados de las prueba 10x10 metros y 45 metros. La muestra fue configurada por un total de 3056 escolares de entre 12 y 13 años (1792 varones y 1264 mujeres). Los resultados mostraron una disminución en el rendimiento del test en ambos sexos para la prueba de 10x10 metros. Por el contrario, no se obtuvieron cambios significativos en la prueba de 45 metros lisos. El aumento de los estilos de vida sedentarios de las sociedades industrializadas, el aumento de la proporción de obesidad, el abuso de las nuevas tecnologías o los nuevos hábitos sociales podrían explicar el empeoramiento de la agilidad en la muestra del estudio

Robust estimation of microbial diversity in theory and in practice

Quantifying diversity is of central importance for the study of structure, function and evolution of microbial communities. The estimation of microbial diversity has received renewed attention with the advent of large-scale metagenomic studies. Here, we consider what the diversity observed in a sample tells us about the diversity of the community being sampled. First, we argue that one cannot reliably estimate the absolute and relative number of microbial species present in a community without making unsupported assumptions about species abundance distributions. The reason for this is that sample data do not contain information about the number of rare species in the tail of species abundance distributions. We illustrate the difficulty in comparing species richness estimates by applying Chao's estimator of species richness to a set of in silico communities: they are ranked incorrectly in the presence of large numbers of rare species. Next, we extend our analysis to a general family of diversity metrics ("Hill diversities"), and construct lower and upper estimates of diversity values consistent with the sample data. The theory generalizes Chao's estimator, which we retrieve as the lower estimate of species richness. We show that Shannon and Simpson diversity can be robustly estimated for the in silico communities. We analyze nine metagenomic data sets from a wide range of environments, and show that our findings are relevant for empirically-sampled communities. Hence, we recommend the use of Shannon and Simpson diversity rather than species richness in efforts to quantify and compare microbial diversity.Comment: To be published in The ISME Journal. Main text: 16 pages, 5 figures. Supplement: 16 pages, 4 figure

Mesonia oceanica sp. Nov., isolated from oceans during the tara oceans expedition, with a preference for mesopelagic waters

Strain ISS653T, isolated from Atlantic seawater, is a yellow pigmented, non-motile, Gram-reaction-negative rod-shaped bac-terium, strictly aerobic and chemoorganotrophic, slightly halophilic (1-15% NaCl) and mesophilic (4-37 °C), oxidase-and catalase-positive and proteolytic. Its major cellular fatty acids are iso-C15:0, iso-C15:0 2-OH, and iso-C17:0 3-OH; the major identified phospholipid is phosphatidylethanolamine and the major respiratory quinone is MK6. Genome size is 4.28 Mbp and DNA G+C content is 34.9 mol%. 16S rRNA gene sequence similarity places the strain among members of the family Flavobacteriaceae, with the type strains of Mesonia phycicola (93.2%), Salegentibacter mishustinae (93.1%) and Mesonia mobilis (92.9%) as closest relatives. Average amino acid identity (AAI) and average nucleotide identity (ANI) indices show highest values with M. mobilis (81% AAI; 78.9% ANI), M. phycicola (76% AAI; 76.3% ANI), Mesonia maritima (72% AAI, 74.9% ANI), Mesonia hippocampi (64% AAI, 70.8% ANI) and Mesonia algae (68% AAI; 72.2% ANI). Phylogenomic analysis using the Up-to-date-Bacterial Core Gene set (UBCG) merges strain ISS653T in a clade with species of the genus Mesonia. We conclude that strain ISS653T represents a novel species of the genus Mesonia for which we propose the name Mesonia oceanica sp. nov., and strain ISS653T (=CECT 9532T=LMG 31236T) as the type strain. A second strain of the species, ISS1889 (=CECT 30008) was isolated from Pacific Ocean seawater. Data obtained throughout the Tara oceans expedition indicate that the species is more abundant in the mesopelagic dark ocean than in the photic layer and it is more frequent in the South Pacific, Indian and North Atlantic oceans

From community approaches to single-cell genomics: the discovery of ubiquitous hyperhalophilic Bacteroidetes generalists

The microbiota of multi-pond solar salterns around the world has been analyzed using a variety of culture-dependent and molecular techniques. However, studies addressing the dynamic nature of these systems are very scarce. Here we have characterized the temporal variation during 1 year of the microbiota of five ponds with increasing salinity (from 18% to >40%), by means of CARD-FISH and DGGE. Microbial community structure was statistically correlated with several environmental parameters, including ionic composition and meteorological factors, indicating that the microbial community was dynamic as specific phylotypes appeared only at certain times of the year. In addition to total salinity, microbial composition was strongly influenced by temperature and specific ionic composition. Remarkably, DGGE analyses unveiled the presence of most phylotypes previously detected in hypersaline systems using metagenomics and other molecular techniques, such as the very abundant Haloquadratum and Salinibacter representatives or the recently described low GC Actinobacteria and Nanohaloarchaeota. In addition, an uncultured group of Bacteroidetes was present along the whole range of salinity. Database searches indicated a previously unrecognized widespread distribution of this phylotype. Single-cell genome analysis of five members of this group suggested a set of metabolic characteristics that could provide competitive advantages in hypersaline environments, such as polymer degradation capabilities, the presence of retinal-binding light-activated proton pumps and arsenate reduction potential. In addition, the fairly high metagenomic fragment recruitment obtained for these single cells in both the intermediate and hypersaline ponds further confirm the DGGE data and point to the generalist lifestyle of this new Bacteroidetes group.This work was supported by the projects CGL2012-39627-C03-01 and 02 of the Spanish Ministry of Economy and Competitiveness, which were also co-financed with FEDER support from the European Union. TG group research is funded in part by a grant from the Spanish Ministry of Economy and Competitiveness (BIO2012-37161), a grant from the Qatar National Research Fund grant (NPRP 5-298-3-086) and a grant from the European Research Council under the European Union’s Seventh Framework Programme (FP/2007-2013)/ERC (grant agreement no. ERC-2012-StG-310325)