Transitioning out of Open Access: A Closer Look at Institutions for Management of Groundwater Rights in France, California, and Spain

[EN] Many regions around the world are transitioning out of open access to groundwater resources in order to tackle over extraction by irrigated agriculture. However, the state has limited capacities to regulate effectively agricultural groundwater use. This paper evaluates how users and public authorities can co-manage groundwater extraction by agriculture. Based on Schlager and Ostrom¿s ¿bundle of rights¿ framework, the paper examines how decisions over access and use of groundwater resources are made in France, Spain and California. The three cases share a common strive to involve groundwater users in decisions over how to reduce over extraction of groundwater resources. However, different choices were made regarding the institutional set-up for user involvement in allocation decisions. The paper presents the diversity of institutional arrangements influencing groundwater allocations in the three cases, and the relative involvement and power of users and public authorities over these institutions. The papers show the different ways in which ¿comanagement¿ may be made operational for managing agricultural groundwater use.This research benefited from funding of the EU H2020 RURECO project (grant agreement 750553) and from Montpellier University I-Site MUSE. This study has also received funding from the eGROUNDWATER project (GA n. 1921), part of the PRIMA programme supported by the European Union's Horizon 2020 research and innovation programmeRouillard, J.; Babbitt, C.; Pulido-Velazquez, M.; Rinaudo, J. (2021). Transitioning out of Open Access: A Closer Look at Institutions for Management of Groundwater Rights in France, California, and Spain. Water Resources Research. 57(4):1-20. https://doi.org/10.1029/2020WR028951S12057

Nuclear localization of the mitochondrial factor HIGD1A during metabolic stress.

Cellular stress responses are frequently governed by the subcellular localization of critical effector proteins. Apoptosis-inducing Factor (AIF) or Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH), for example, can translocate from mitochondria to the nucleus, where they modulate apoptotic death pathways. Hypoxia-inducible gene domain 1A (HIGD1A) is a mitochondrial protein regulated by Hypoxia-inducible Factor-1α (HIF1α). Here we show that while HIGD1A resides in mitochondria during physiological hypoxia, severe metabolic stress, such as glucose starvation coupled with hypoxia, in addition to DNA damage induced by etoposide, triggers its nuclear accumulation. We show that nuclear localization of HIGD1A overlaps with that of AIF, and is dependent on the presence of BAX and BAK. Furthermore, we show that AIF and HIGD1A physically interact. Additionally, we demonstrate that nuclear HIGD1A is a potential marker of metabolic stress in vivo, frequently observed in diverse pathological states such as myocardial infarction, hypoxic-ischemic encephalopathy (HIE), and different types of cancer. In summary, we demonstrate a novel nuclear localization of HIGD1A that is commonly observed in human disease processes in vivo

Participatory analysis for adaptation to climate change in Mediterranean agricultural systems: possible choices in process design (versão Pre Print)

There is an increasing call for local measures to adapt to climate change, based on foresight analyses in collaboration with actors. However, such analyses involve many challenges, particularly because the actors concerned may not consider climate change to be an urgent concern. This paper examines the methodological choices made by three research teams in the design and implementation of participatory foresight analyses to explore agricultural and water management options for adaptation to climate change. Case studies were conducted in coastal areas of France, Morocco, and Portugal where the groundwater is intensively used for irrigation, the aquifers are at risk or are currently overexploited, and a serious agricultural crisis is underway. When designing the participatory processes, the researchers had to address four main issues: whether to avoid or prepare dialogue between actors whose relations may be limited or tense; how to select participants and get them involved; how to facilitate discussion of issues that the actors may not initially consider to be of great concern; and finally, how to design and use scenarios. In each case, most of the invited actors responded and met to discuss and evaluate a series of scenarios. Strategies were discussed at different levels, from farming practices to aquifer management. It was shown that such participatory analyses can be implemented in situations which may initially appear to be unfavourable. This was made possible by the flexibility in the methodological choices, in particular the possibility of framing the climate change issue in a broader agenda for discussion with the actors

Type I collagen limits VEGFR-2 signaling by a SHP2 protein-tyrosine phosphatase-dependent mechanism 1.

During angiogenesis, a combined action between newly secreted extracellular matrix proteins and the repertoire of integrins expressed by endothelial cells contributes in the regulation of their biological functions. Extracellular matrix-engaged integrins influence tyrosine kinase receptors, thus promoting a regulatory cross-talk between adhesive and soluble stimuli. For instance, vitronectin has been reported to positively regulate VEGFR-2. Here, we show that collagen I downregulates VEGF-A-mediated VEGFR-2 activation. This activity requires the tyrosine phosphatase SHP2, which is recruited to the activated VEGFR-2 when cells are plated on collagen I, but not on vitronectin. Constitutive expression of SHP2(C459S) mutant inhibits the negative role of collagen I on VEGFR-2 phosphorylation. VEGFR-2 undergoes internalisation, which is associated with dynamin II phosphorylation. Expression of SHP2(C459S) impairs receptor internalisation suggesting that SHP2-dependent dephosphorylation regulates this process. These findings demonstrate that collagen I in provisional extracellular matrix surrounding nascent capillaries triggers a signaling pathway that negatively regulates angiogenesis

Determinants of bone damage: An ex-vivo study on porcine vertebrae

Bone\u2019s resistance to fracture depends on several factors, such as bone mass, microarchitecture, and tissue material properties. The clinical assessment of bone strength is generally performed by Dual-X Ray Photon Absorptiometry (DXA), measuring bone mineral density (BMD) and trabecular bone score (TBS). Although it is considered the major predictor of bone strength, BMD only accounts for about 70% of fragility fractures, while the remaining 30% could be described by bone \u201cquality\u201d impairment parameters, mainly related to tissue microarchitecture. The assessment of bone microarchitecture generally requires more invasive techniques, which are not applicable in routine clinical practice, or X-Ray based imaging techniques, requiring a longer post-processing. Another important aspect is the presence of local damage in the bony tissue that may also affect the prediction of bone strength and fracture risk. To provide a more comprehensive analysis of bone quality and quantity, and to assess the effect of damage, here we adopt a framework that includes clinical, morphological, and mechanical analyses, carried out by means of DXA, \u3bcCT and mechanical compressive testing, respectively. This study has been carried out on trabecular bones, taken from porcine trabecular vertebrae, for the similarity with human lumbar spine. This study confirms that no single method can provide a complete characterization of bone tissue, and the combination of complementary characterization techniques is required for an accurate and exhaustive description of bone status. BMD and TBS have shown to be complementary parameters to assess bone strength, the former assessing the bone quantity and resistance to damage, and the latter the bone quality and the presence of damage accumulation without being able to predict the risk of fracture

Actividad glutatión peroxidasa en vacas lecheras sin suplementar y suplementadas con selenio

The aim of this work was to evaluate glutathione peroxidase (GPX) activity levels in dairy cows supplemented and not supplemented with intra-ruminal selenium (Se) boluses. The GPX activity of 96 clinically healthy primiparous and multiparous Holstein dairy cows, from a commercial establishment in Centeno´s town, Santa Fe Province (Argentina), was assessed. According to the individual GPX determinations, two categories of cows were defined taking 130 U/gHb as the cut value. The cows in each category were randomly divided in two subgroups, one of which received an intra-ruminal bolus of long-acting Se (Permatrace®) while the other remained unsupplemented. Next, two new enzymatic activity determinations were made: ante-partum (between 30 and 15 days before calving) and post-partum (between 30 and 45 after calving). The 35.4% of the cows presented enzyme activity values lower than the cut value. Cows with low basal values of GPX activity not supplemented with Se boluses did not significantly modify the basal values. In cows with low basal values of GPX activity and supplemented with Se, a significant increase in enzymatic activity was observed. It is concluded that a significant proportion of cows have low GPX activity, enzymatic activity levels are modified according to the moment of evaluation in relation to calving and that supplementation with intra-ruminal Se is an effective strategy to increase the enzyme activity.El objetivo del presente trabajo fue evaluar los niveles de la actividad glutatión-peroxidasa(GPX) en vacas lecheras suplementadas y sin suplementar con selenio (Se) intra-ruminal.Se determinó la actividad GPX de 96 vacas Holstein primíparas y multíparas, clínicamentesanas, procedentes de un establecimiento comercial de la localidad de Centeno, Provincia deSanta Fe (Argentina). Se definieron dos categorías de reses según las determinaciones individuales de GPX tomando como valor de corte 130 U/gHb. Los animales de cada categoría se dividieron aleatoriamente en dos subgrupos, uno de los cuales recibió un bolo intraruminalde Se de larga acción (Permatrace®) mientras que el otro permaneció sin suplementar. Acontinuación, se efectuaron dos nuevas determinaciones de la actividad enzimática: preparto(entre los 30 y los 15 días previos al parto) y posparto (entre los 30 y los 45 posteriores alparto). El 35,4% de las vacas presentó valores de actividad de la enzima menores al valor decorte. Los animales con bajos valores basales de actividad GPX no suplementadas con bolosde Se, no modificaron significativamente los datos basales. En las vacas con bajos valoresbasales de actividad GPX y suplementadas con Se, se observó un aumento significativo de laactividad enzimática. Se concluye que una proporción importante de animales presenta bajaactividad GPX, que los niveles de las enzimas se modifican según el momento de la evaluaciónen relación al tiempo del parto, y que la suplementación con bolos de Se intra-ruminalconstituye una estrategia efectiva para aumentar la actividad de la enzima

Inter-basin transfers as a supply option: the end of an era?

International audienceThis chapter discusses the evolving role of interbasin transfers (IBT) in urban water management. After providing an historical overview of IBT development, the chapter describes how IBTs are challenged by a change in the technological and socio-economic context. The emergence of alternative technologies, such as desalination, wastewater reclamation and reuse, or managed artificial groundwater recharge is reducing the attractiveness of IBTs. Water utilities are also becoming increasingly aware that water conservation programs can save volumes of water at a much cheaper cost than IBT. Various international examples are used to show that IBTs trigger increasing concerns from communities involved or affected, in particular related to the environmental impact on donor and receiving river basins, the economic impact on donor regions, the impact on local cultures and livelihoods, how costs and benefits are distributed (social justice), and issues related to public participation. The chapter concludes by looking ahead at new and more efficient uses of existing IBTs. As conjunctive use management approaches gain support, IBTs will be operated in conjunction with aquifer storage and recovery schemes. They will probably also support the development of emerging water markets, in particular during drought years