Liderazgo transformacional como estrategia para lograr el compromiso organizacional en la Gerencia Regional de Educación Lambayeque, 2022

El presente informe de investigación tiene como objetivo: Proponer estrategias de liderazgo transformacional para lograr el compromiso organizacional en la Gerencia Regional de Educación Lambayeque, 2022. La investigación tiene como hipótesis, H1: El liderazgo transformacional como estrategia lograra el Compromiso Organizacional en los Colaboradores de la Gerencia Regional de Educación Lambayeque.2022 La metodología que se realizo es de tipo propositiva, con un enfoque cuantitativo y un diseño no experimental. Según la investigación, para la independiente se aplicó una guía de observación y así determinar el grado de liderazgo transformacional con cada dimensión donde determino un escaso liderazgo por parte del personal dentro de la entidad y para la variable dependiente se aplicó una encuesta con un cuestionario de 21 preguntas con respecto al (Compromiso Organizacional). Donde la población estuvo conformada por 92 colaboradores, por lo que se tomó a toda la población como muestra, por ser finita. Asimismo para el procesamiento de los datos se utilizará el programa SPSS 25, lo cual permitirá obtener resultados confiables, entre los resultados más relevantes se encontró que el 38% de los subordinados están en desacuerdo, considerando que no se sienten identificados con la entidad, lo cual está provocando un ambiente laboral muy pesado y con pocas ganas de seguir ejerciendo sus funciones, por otro lado el 44% de los subordinados manifiestan estar en desacuerdo, lo que implica que la institución no prioriza las capacitaciones para mejorar la eficiencia en sus trabajadores, lo cual es un problema pues la entidad corre el riesgo de que sus trabajadores desarrollen mal sus actividades, perjudicando en gran medida la productividad institucional, llegando a la conclusión proponer diferentes actividades estratégicas con el propósito de solucionar los problemas que la institución está pasando actualmente, para de ese modo poder lograr el compromiso organizacional lo cual dará lugar a que los colaboradores se sientan identificados con la entidad, y sobre todo comprometidos con los objetivos que la entidad estatal persigue.TesisGestión empresarial y emprendimient

A new sunburned apple category browning under conventional and organic management: phenolic compounds and antioxidant capacity in cold storage

The effect of mild sunburn damage on three apple cultivars: Brookfield®, Granny Smith and Fuji from conventional and organic orchards in the 6th Region of Chile were evaluated in 2012/2013. Total and specific phenols, antioxidant capacity and ripeness of the fruit were assessed at harvest and different time of conventional cold storage: after one, two and four months. According to the results, the peel of all the cultivars have their own property and the response to sunburn damage is independent of the type of management. Phenolic compounds content in the peel of sun-damaged apples at harvest was twice as high in comparison with the peel of healthy fruit. After four months, the apples with mild damage under this conditionhad higher phenolic compounds and antioxidant content than healthy fruit at harvest. Considering these successful results, a new category of “sunny – apple”, we propose that has a higher content of antioxidantcompounds

Modelo de simulación del proceso de matrícula de la Universidad Nacional del Altiplano - Puno

En la presente investigación se plantea reducir el tiempo de espera en el proceso de matrículas de la Universidad Nacional del Altiplano - Puno. Los problemas en el sistema de matrícula es una realidad evidente a la percepción actual, por lo que se requiere cambiar este contexto. Una de las formas de resolver el problema del sistema de matrícula, consiste en transformar las situaciones del mundo real en los modelos matemáticos, para luego solucionar mediante la implementación de herramientas tecnológicas, que alberga elementos cuantitativos para la toma de decisiones y el sistema de gestión de calidad ISO 9001. Para ello está disponible las tecnologías actuales que nos permite resolver problemas complejos en poco tiempo, para lo cual se ha propuesto como objetivo, Determinar la simulación del proceso de matrícula de la Universidad Nacional del Altiplano – Puno. Cuya metodología es de la categoría de investigación cuantitativa a un nivel explicativo con un solo grupo, cuya validez se efectúa mediante el juicio de expertos, asimismo para lo cual se ha utilizado la aplicación de la metodología de simulación, que consiste en la gestión de los procesos y el control de los procesos, con la cual se llegó a las siguientes conclusiones: a) que los estudiantes están en desacuerdo con el proceso de matrícula actual, b) al simular el proceso de matrícula se puede observar que sí mejora el tiempo en el proceso de matrícula c) se ha concluido que con la simulación se ha mejorado el tiempo en el proceso de matrícula.Tesi

Diseño de un plan estratégico de marketing para potenciar los servicios administrativos del instituto de postgrado y educación continua de la UNEMI.

El trabajo de esta propuesta está fundamentado en el estudio de las Estrategias de Marketing en la Gestión Administrativa del instituto de Postgrado y Educación Continua (IPEC) de la Universidad Estatal de Milagro (UNEMI), nuestra iniciativa de investigación parte que al percatarnos de que en el IPEC, no están aplicado un Plan Estratégico de Marketing que permita mejorar su administración y su servicio que conlleve a posicionarse como marca reconocida en el mercado beneficiando educacionalmente a los profesionales. Este proyecto de grado se encuentra detallado en cinco capítulos detallados de la siguiente manera: la problemática, objetivos, delimitación, formulación y su debida justificación, hemos tomado como referencia Institutos de Postgrado de Universidades Nacionales y Extranjeras que apliquen Marketing como referencia y aporte a este proyecto, para formular las hipótesis y variables. Las encuestas fueron hechas a nuestra población y muestra que son el personal Administrativo que trabaja en el IPEC, también como soporte de la investigación se realizó encuestas a los profesionales que están cursando una maestría en el IPEC y también se procedió con una entrevista al Gerente del Banco de Machala como ayuda a nuestro proyecto. El resultado es de gran beneficio para el Instituto el aplicar un Plan Estratégico de Marketing que permita potencia sus servicios. Realizamos un pequeño cuadro financiero en el que se establece los que gastamos en la elaboración de esta tesis. También se procedió a indicar varias actividades que el IPEC debe hacer si procede a realizar un Plan Estratégico de Marketing

Cell adhesion and Ca2+ signaling activity in stably transfected trypanosoma cruzi epimastigotes expressing the metacyclic stage-specific surface molecule gp82

Metacyclic trypomastigotes of Trypanosoma cruzi express a developmentally regulated 82-kDa surface glycoprotein (gp82) that has been implicated in host cell invasion. gp82-mediated interaction of metacyclic forms with target cells induces in both cells activation of the signal transduction pathways, leading to intracellular Ca2+ mobilization, which is required for parasite internalization. Noninfective epimastigotes do not express detectable levels of gp82 and are unable to induce a Ca2+ response. We stably transfected epimastigotes with a T. cruzi expression vector carrying the metacyclic stage gp82 cDNA. These transfectants produced a functional gp82, which bound to and triggered a Ca2+ response in HeLa cells, in the same manner as the metacyclic trypomastigote gp82. Such properties were not found in epimastigotes transfected with the plasmid vector alone. Epimastigotes expressing gp82 on the surface adhered to HeLa cells but were not internalized. Treatment of gp82-expressing epimastigotes with forskolin, an activator of adenylyl cyclase that increases the metacyclic trypomastigote entry into target cells, did not promote parasite internalization. P175, an intracellular tyrosine phosphorylated protein, which appears to play a role in gp82-dependent signaling cascade in metacyclic forms, was undetectable in epimastigotes, either transfected or not with pTEX-gp82. Overall, our results indicate that gp82 is required but not sufficient for target cell invasion.Universidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Biofis, BR-04023062 São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Biofis, BR-04023062 São Paulo, BrazilWeb of Scienc

Deletion of inducible nitric-oxide synthase in leptin-deficient mice improves brown adipose tissue function

Abstract Background: Leptin and nitric oxide (NO) on their own participate in the control of non-shivering thermogenesis. However, the functional interplay between both factors in this process has not been explored so far. Therefore, the aim of the present study was to analyze the impact of the absence of the inducible NO synthase (iNOS) gene in the regulation of energy balance in ob/ob mice. Methods and Findings: Double knockout (DBKO) mice simultaneously lacking the ob and iNOS genes were generated, and the expression of molecules involved in the control of brown fat cell function was analyzed by real-time PCR, western-blot and immunohistochemistry. Twelve week-old DBKO mice exhibited reduced body weight (p,0.05), decreased amounts of total fat pads (p,0.05), lower food efficiency rates (p,0.05) and higher rectal temperature (p,0.05) than ob/ob mice. Ablation of iNOS also improved the carbohydrate and lipid metabolism of ob/ob mice. DBKO showed a marked reduction in the size of brown adipocytes compared to ob/ob mutants. In this sense, in comparison to ob/ob mice, DBKO rodents showed an increase in the expression of PR domain containing 16 (Prdm16), a transcriptional regulator of brown adipogenesis. Moreover, iNOS deletion enhanced the expression of mitochondria-related proteins, such as peroxisome proliferatoractivated receptor c coactivator-1 a (Pgc-1a), sirtuin-1 (Sirt-1) and sirtuin-3 (Sirt-3). Accordingly, mitochondrial uncoupling proteins 1 and 3 (Ucp-1 and Ucp-3) were upregulated in brown adipose tissue (BAT) of DBKO mice as compared to ob/ob rodents. Conclusion: Ablation of iNOS improved the energy balance of ob/ob mice by decreasing food efficiency through an increase in thermogenesis. These effects may be mediated, in part, through the recovery of the BAT phenotype and brown fat cell function improvement

Oxidative Cleavage of Cellobiose by Lytic Polysaccharide Monooxygenase (LPMO)-Inspired Copper Complexes

Correction published on October 23, 2020 https://doi.org/10.1021/acsomega.0c04910The potentially tridentate ligand bis[(1-methyl-2-benzimidazolyl)ethyl]amine (2BB) was employed to prepare copper complexes [(2BB)CuI]OTf and [(2BB)CuII(H2O)2](OTf)2 as bioinspired models of lytic polysaccharide copper-dependent monooxygenase (LPMO) enzymes. Solid-state characterization of [(2BB)CuI]OTf revealed a Cu(I) center with a T-shaped coordination environment and metric parameters in the range of those observed in reduced LPMOs. Solution characterization of [(2BB)CuII(H2O)2](OTf)2 indicates that [(2BB)CuII(H2O)2]2+ is the main species from pH 4 to 7.5; above pH 7.5, the hydroxo-bridged species [{(2BB)CuII(H2O)x}2(μ-OH)2]2+ is also present, on the basis of cyclic voltammetry and mass spectrometry. These observations imply that deprotonation of the central amine of Cu(II)-coordinated 2BB is precluded, and by extension, amine deprotonation in the histidine brace of LPMOs appears unlikely at neutral pH. The complexes [(2BB)CuI]OTf and [(2BB)CuII(H2O)2](OTf)2 act as precursors for the oxidative degradation of cellobiose as a cellulose model substrate. Spectroscopic and reactivity studies indicate that a dicopper(II) side-on peroxide complex generated from [(2BB)CuI]OTf/O2 or [(2BB)CuII(H2O)2](OTf)2/H2O2/NEt3 oxidizes cellobiose both in acetonitrile and aqueous phosphate buffer solutions, as evidenced from product analysis by high-performance liquid chromatography-mass spectrometry. The mixture of [(2BB)CuII(H2O)2](OTf)2/H2O2/NEt3 results in more extensive cellobiose degradation. Likewise, the use of both [(2BB)CuI]OTf and [(2BB)CuII(H2O)2](OTf)2 with KO2 afforded cellobiose oxidation products. In all cases, a common Cu(II) complex formulated as [(2BB)CuII(OH)(H2O)]+ was detected by mass spectrometry as the final form of the complex

The chemical compositions of essential oils derived from cryptocarya alba and laurelia sempervirens possess antioxidant, antibacterial and antitumoral activity potential

Cryptocarya alba (Peumo; CA) and Laurelia sempervirens (Laurel; LS) are herbs native to the Chilean highlands and have historically been used for medicinal purposes by the Huilliches people. In this work, the essential oils were extracted using hydrodistillation in Clevenger apparatus and analyzed by GC-MS to determine their composition. The antioxidant capacity (AC) was evaluated in vitro. The cytotoxicity was determined using cell line cultures both non tumoral and tumoral. The toxicity was determined using the nematode Caenorhabditis elegans. The antimicrobial activity was evaluated against 52 bacteria using the agar disc diffusion method and the minimum inhibitory concentrations (MICs) were determined. The principal compounds found in C. alba essential oil (CA_EO) were α-terpineol (24.96%) and eucalyptol (21.63%) and were isazafrol (91.9%) in L. sempervirens essential oil (LS_EO). Both EOs showed antioxidant capacity in vitro. Both EO showed antibacterial activity against bacteria using. LS_EO showed more inhibitory effect on these cell lines respect to CA_EO. Both EOs showed toxicity against the nematode C. elegans at 3.12–50 mg/mL. The essential oils of CA and LS have an important bioactive potential in their antioxidant, antibacterial and cytotoxicity activity. Both essential oils could possibly be used in the field of natural medicine, natural food preservation, cosmetics, sanitation and plaguicides among others.Fil: Touma, Jorge. Universidad Diego Portales; ChileFil: Navarro, Myriam. Universidad Diego Portales; ChileFil: Sepúlveda, Betsabet. Universidad de Chile; ChileFil: Pavon, Alequis. Universidad Autónoma de Chile; ChileFil: Corsini, Gino. Universidad Autónoma de Chile; ChileFil: Fernández, Katia. Universidad Diego Portales; ChileFil: Quezada, Claudia. Universidad Austral de Chile; ChileFil: Torres, Angelo. Universidad Austral de Chile; ChileFil: Larrazabal Fuentes, María José. Universidad de Antofagasta; ChileFil: Paredes, Adrian. Universidad de Antofagasta; ChileFil: Neira, Ivan. Universidad de Antofagasta; ChileFil: Ferrando, Matías. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Bruna, Flavia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Venegas, Alejandro. Universidad Diego Portales; ChileFil: Bravo, Jessica. Universidad Diego Portales; Chil

The effect of occupational exposure to solar ultraviolet radiation on malignant skin melanoma and non- melanoma skin cancer: a systematic review and meta-analysis from the WHO/ILO Joint Estimates of the Work-related Burden of Disease and Injury

A systematic review and meta-analysis of studies were conducted reporting on the association between occupational exposure to solar ultraviolet radiation (UVR) and both malignant skin melanoma (melanoma) and non-melanoma skin cancer (NMSC), with the aim of enabling the estimation of the numbers of deaths and disability-adjusted life years from melanoma and NMSC attributable to occupational exposure to solar UVR, for the development of the World Health Organization (WHO)/International Labour Organization (ILO) Joint Estimates of the Work-related Burden of Disease and Injury (WHO/ILO Joint Estimates). A protocol was developed and published, applying the Navigation Guide as an organizing systematic review framework where feasible. Electronic bibliographic databases were searched for potentially relevant records; electronic grey literature databases and organizational websites were also searched, reference lists of previous systematic reviews and included study records were hand-searched, and additional experts were consulted. Randomized controlled trials and cohort, case–control and other non-randomized studies were included that estimated the effect of any occupational exposure to solar UVR, compared with no occupational exposure to solar UVR, on melanoma (excluding melanoma of the lip or eye) or NMSC prevalence, incidence or mortality. At least two reviewers independently screened titles and abstracts against the eligibility criteria at a first stage and full texts of potentially eligible records at a second stage. Adjusted relative risks were combined using random-effects meta-analysis. Two or more reviewers assessed the risk of bias, quality of evidence and strength of evidence. Fifty-three (48 case–control, three case–case and two cohort) eligible studies were found, published in 62 study records, including over 457 000 participants in 26 countries of three WHO regions (Region of the Americas, European Region and Western Pacific Region), reporting on the effect on melanoma or NMSC incidence or mortality. No studies on the prevalence of melanoma or NMSC were found. In most studies, exposure was self-reported in questionnaires during interviews and the health outcome was assessed via physician diagnosis based on biopsy and histopathological confirmation. The risk of bias of the body of evidence was judged to be generally “probably low”, although there were some concerns regarding risks of exposure misclassification bias, detection bias and confounding. The main meta-analyses of relevant case–control studies revealed a relative risk (RR) of melanoma and NMSC incidence of 1.45 (95% confidence interval (CI): 1.08–1.94; I2 = 81%) and 1.60 (95% CI: 1.21–2.11; I2 = 91%), respectively. No statistically significant differences in risk of melanoma and NMSC incidence were found when conducting subgroup analyses by WHO region, and no differences in risk of NMSC incidence in a subgroup analysis by sex. However, in a subgroup analysis by NMSC subtype, the increased risk of basal cell carcinoma (RR: 1.50; 95% CI: 1.10–2.04; 15 studies) was probably lower (P = 0.05 for subgroup differences) than the increased risk for squamous cell carcinoma (RR: 2.42; 95% CI: 1.66–3.53; 6 studies). The sensitivity analyses found that effect estimates of NMSC incidence were significantly higher in studies with any risk of bias domain rated as “high” or “probably high” compared with studies with only a “low” or “probably low” risk of bias, and in studies not reporting the health outcome by International Statistical Classification of Diseases and Related Health Problems (ICD) code compared with the two studies reporting ICD codes. The quality of available evidence of the effect of any occupational exposure to solar UVR on melanoma incidence and mortality and on NMSC mortality was rated as “low”, and the quality of evidence for NMSC incidence was rated as “moderate”. The strength of the existing bodies of evidence reporting on occupational exposure to solar UVR was judged as “inadequate evidence for harmfulness” for melanoma mortality and NMSC mortality. For the health outcome of melanoma incidence, the strength of evidence was judged as “limited evidence for harmfulness”, that is, a positive relationship was observed between exposure and outcome where chance, bias and confounding cannot be ruled out with reasonable confidence. For the health outcome of NMSC incidence, the strength of evidence was judged as “sufficient evidence of harmfulness”, that is, a positive relationship is observed between exposure and outcome where chance, bias and confounding can be ruled out with reasonable confidence. The 2009 International Agency for Research on Cancer classification of solar UVR as a Group 1 carcinogen that causes cutaneous melanoma and NMSC is a compelling attribute for the strength of evidence on occupational exposure to solar UVR and skin cancer incidence. Producing estimates for the burden of NMSC attributable to occupational exposure to solar UVR appears evidence-based (while acknowledging the limitations of the bodies of evidence), and the pooled effect estimates can be used as input data for the WHO/ILO Joint Estimates