A comparative analysis of genetic diversity in Portuguese grape germplasm from ampelographic collections fit for quality wine production

Grapevine cultivars diversity is vast and full of synonyms and homonyms. Up to few decades ago characterization of grapevine was based on morphological characters. In the last decades, molecular markers were developed and have been used as tools to study genetic diversity in a range of different plant species. Fifty-six Portuguese accessions representative of ‘Vinhos Verdes’ and ‘Douro’ Controlled Designations of Origin (DOC) were analysed through DNA fingerprints generated by Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeat (ISSR). The study aimed to compare the effectiveness of RAPD and ISSR molecular techniques in the detection of synonyms, homonyms and misnames. RAPD and ISSR analysis enabled the detection of 36 different band patterns, reducing in about 36% the initial material. Several accessions grown under different names, between and within collections, were confirmed as the same genotype, namely Gouveio/Verdelho, Sousão Douro/Vinhão and Arinto Oeste/Pedernã. Similarly, some homonyms/misnames were also identified, namely within Azal Tinto and Rabigato accessions. RAPD and ISSR markers revealed to be adequate molecular techniques for grapevine varieties fingerprinting with advantages over other molecular procedures, contributing for a good management of grapevine collections

Screening of worldwide cowpea collection to drought tolerant at a germination stage

Supplementary material related to this article can be found, in the online version, at doi: https://doi.org/10.1016/j.scienta.2018.11.082.Global warming has an increasing impact on the availability of water for agriculture. Crops tolerant to high temperatures and drought, such as cowpea (Vigna unguiculata L. Walp.), have an added value in the near future. The main objective of this study was to evaluate the effect of drought on seed germination and seedling emergence of cowpea genotypes, in order to screen the most tolerant genotypes. Seeds from 58 cowpea genotypes all over the world were submitted to two stress conditions, induced by PEG-6000 (corresponding to osmotic potentials of -0.75 bars and -1.5 bars). Germination and seedling growth parameters, vigor index and proline content were determined to assess drought tolerance. The results revealed significant differences of all parameters among genotypes after treatments and interaction of both. Water stress caused a general decrease in germination and seedling growth, while an increase in proline content was observed. A high variation of drought responses were detected among genotypes, being possible to select seven genotypes (C11, C18, C44, C46, C47, C50 and C54) as tolerant to drought at germination stage. These results will be useful to select the best suitable parents for insertion in future breeding programs.This study was supported by EUROLEGUME project. This project has received funding from the European Union's Seventh Framework Programme for research, technological development and demonstration under grant agreement no 613781. European Investment Funds by FEDER/COMPETE/POCI - Operational Competitiveness and Internationalization Programme, under Project POCI-01-0145-FEDER-006958 and National Funds by FCT - Portuguese Foundation for Science and Technology, under the projects UID/AGR/04033/2013 and UID/AGR/04046/2013

México: El Impuesto A Las Ganancias De Capital Y El Rendimiento.

Art.;15 pA través de los años y sobre todo a partir de la crisis financiera en 2008 desatada en Estados Unidos generando efectos negativos en la economía internacional, mucho se ha analizado y discutido acerca de los efectos que trae consigo la especulación financiera por parte de los inversionistas en la economía nacional y a nivel internacional, en nuestro país el gobierno federal ha establecido para las ganancias de capital, a través de reformas fiscales, un gravamen a este tipo de ingresos, esto con el objeto de dotar de elementos de control sobre las inversiones frenando la especulación, así como homologar tratamiento de las ganancias de capital al que se observa en el contexto internacional, aunado a que con dichas medidas se espera obtener una mayor recaudación de impuesto (Impuesto Sobre la Renta, ISR), gravando en su totalidad este tipo de ingresos, con este trabajo se analizar el impacto financiero que tiene el inversionista al obtener ingresos por enajenación de acciones y por dividendos percibidos, con la aplicación de la nueva ley del ISR aplicable a partir del 1° de enero de 2014 haciendo el comparativo contra la Ley vigente al 31 de diciembre de 2013.Universidad Libre Seccional Pereir

Profile of students registered in nursing auxiliary and technician courses of the Nursing Worker Professionalization Project (PROFAE) in Rio de Janeiro - Brazil

O objetivo deste trabalho é apresentar o perfil dos alunos ingressos nos Cursos de Qualificação Profissional para Auxiliar de Enfermagem e Complementação para Técnico de Enfermagem do Projeto de Profissionalização dos Trabalhadores da Área de Enfermagem (PROFAE). Esta é uma pesquisa quantitativa, desenvolvida no Estado do Rio de Janeiro, no período de agosto de 2004 a janeiro de 2005, com aplicação de 1400 questionários. Os dados foram tratados com estatística descritiva, sob a forma de freqüência simples e percentual. Depois de tabulados, eles foram divididos nas seguintes categorias: dados sociodemográficos, formação escolar, perfil familiar, hábitos, atuação profissional, faixa salarial, expectativas sobre o PROFAE e a Enfermagem, dificuldades para participar do Projeto, e processo ensino-aprendizagem. Os alunos/trabalhadores formados pelo PROFAE tendem a melhorar a qualidade da atenção hospitalar e ambulatorial, contribuindo para a dinamização do mercado de trabalho no setor de saúde.Este trabajo busca presentar el perfil de los alumnos que ingresaron a los cursos de Calificación Profesional para Auxiliar de Enfermería y complementación para Técnico de Enfermería del Proyecto de Profesionalización de los trabajadores del Área de Enfermería. Esta es una investigación cuantitativa, desarrollada en el Estado de Rio de Janeiro, en el período de agosto del 2004 a enero del 2005, con aplicación de 1.400 cuestionarios. Los datos fueron tratados con estadística descriptiva, bajo la forma de frecuencia simple y porcentaje. Después de tabular los datos, fueron divididos en las siguientes categorías: datos sociodemográficos, formación escolar, perfil familiar, hábitos, actuación profesional, piso salarial, expectativas acerca de PROFAE y de la enfermería, dificultades para participar del Proyecto y proceso de enseñanza-aprendizaje. Los alumnos/trabajadores formados por el PROFAE tienden a mejorar la calidad de la atención hospitalaria y ambulatoria, contribuyendo con la dinámica del mercado de trabajo en el sector de la salud.This paper aims to present a profile of students registered in the Professional Training Courses for Nursing Auxiliaries and Complementation Courses for Nursing Technicians of the Nursing Worker Professionalization Project - PROFAE. This quantitative study was carried out in the State of Rio de Janeiro, Brazil, from August 2004 to January 2005, through the application of 1,400 questionnaires. Data analysis used descriptive statistics, with simple frequencies and percentages. After tabulation, data were divided in the following categories: sociodemographic data, educational background, family profile, habits, professional activity, salary range, expectations about PROFAE and nursing, difficulties to participate in the project and the teaching-learning process. Students/workers graduated from the PROFAE program tend to improve the quality of hospital and outpatient care, contributing to labor market dynamics in the health sector

Pervasive hybridization with local wild relatives in Western European grapevine varieties

Grapevine (Vitis vinifera L.) diversity richness results from a complex domestication history over multiple historical periods. Here, we used whole-genome resequencing to elucidate different aspects of its recent evolutionary history. Our results support a model in which a central domestication event in grapevine was followed by postdomestication hybridization with local wild genotypes, leading to the presence of an introgression signature in modern wine varieties across Western Europe. The strongest signal was associated with a subset of Iberian grapevine varieties showing large introgression tracts. We targeted this study group for further analysis, demonstrating how regions under selection in wild populations from the Iberian Peninsula were preferentially passed on to the cultivated varieties by gene flow. Examination of underlying genes suggests that environmental adaptation played a fundamental role in both the evolution of wild genotypes and the outcome of hybridization with cultivated varieties, supporting a case of adaptive introgression in grapevine.info:eu-repo/semantics/publishedVersio

FINANCE GAME: UM JOGO DE APOIO À EDUCAÇÃO FINANCEIRA

Vivemos hoje um momento em que se explora cada vez mais o consumismo excessivo e em que muitas pessoas gastam mais do que ganham, vivendo em um constante endividamento. A educação financeira se mostra extremamente necessária desde a infância, para que decisões sensatas e planejadas possam ser tomadas. Diante desse cenário, este artigo apresenta um jogo educativo de apoio à educação financeira, que objetiva apoiar a tomada de decisões visando o equilíbrio financeiro e a qualidade de vida. Para seu desenvolvimento foi utilizada a metodologia XisOA. O jogo foi bem avaliado por uma especialista da área de educação, que também apontou aspectos a serem melhorados, e, da mesma forma, foi bem avaliado na opinião de algumas crianças que o jogaram

Breastfeeding, deleterious oral habits and malocclusion in 5-year-old children in São Pedro, SP, Brazil

OBJECTIVE: To estimate the frequency of malocclusion and their associations with the type and period of breastfeeding, deleterious oral habits, and information received by mothers during the pre-natal period, in 5-year-old children attending municipal daycare centers. METHODS: The sample consisted of 162 children resident in the municipality of São Pedro, SP, Brazil. In an interview with each of the mothers, information was collected about the time and form of breastfeeding, presence of deleterious habits, and information the mother received during the pre-natal period. The epidemiological exam was performed at the daycare center facilities by a single, previously calibrated examiner, under direct lighting. The following variables were evaluated: presence and severity of malocclusion [slight overcrowding and spacing (OS)], open occlusal relationship (open bite) (OPB), vertical overlap (over bite) (OVB), uni- or bilateral cross bite (CB), positive overjet (OV) and the primary second molar terminal plane relationship (TPR)]. Data analysis consisted of univariate analysis (chi-square test) and multiple logistic regressions. RESULTS: The prevalence of malocclusions was 95.7% (OS = 22.8%; OPB = 24.7%; OVB = 20.4%; CB = 14.8%; and OV = 13.0%). In TPR the straight terminal plane was predominant (85.0%). Among the deleterious oral habits, the use of a pacifier was the only risk indicator (OR = 5.25; p = 0.001) for open occlusal relationship (open bite) in children that used it for over three years, detected in the logistic regressions. CONCLUSION: The prevalence of malocclusions and deleterious oral habits in the studied sample was high. Children that used a pacifier for over three years showed greater probability of presenting with open occlusal relationship (open bite).OBJETIVO: estimar a frequência de oclusopatias e suas associações com o tipo e o período de amamentação, hábitos bucais deletérios e informações recebidas pelas mães no pré-natal, em crianças com cinco anos de idade que frequentavam creches municipais. MÉTODOS: a amostra consistiu de 162 crianças residentes no município de São Pedro, SP. Em entrevista com cada mãe, informações sobre o tempo e a forma de aleitamento, a presença de hábitos deletérios, e orientações recebidas pela mãe durante o pré-natal foram coletadas. O exame epidemiológico foi realizado nas dependências das creches, por um único examinador, previamente calibrado, sob iluminação direta. As seguintes variáveis foram avaliadas: presença e severidade de oclusopatias [ligeiro apinhamento e espaçamento (AE), mordida aberta (MA), sobremordida (SM), mordida cruzada uni ou bilateral (MC), overjet positivo (OV) e relação terminal dos segundos molares decíduos (RTM)]. A análise dos dados consistiu de análise univariada (teste qui-quadrado) e de regressão logística múltipla. RESULTADOS: a prevalência de oclusopatias foi de 95,7% (AE = 22,8%; MA = 24,7%; SM = 20,4%; MC = 14,8%; e OV = 13,0%). Na RTM, o terminal reto foi predominante (85,0%). Dentre os hábitos bucais deletérios, o uso de chupeta foi o único indicador de risco (OR = 5,25; p = 0,001) para mordida aberta em crianças que a utilizaram por mais de três anos, detectado nas regressões logísticas. CONCLUSÃO: a prevalência de oclusopatias e de hábitos bucais deletérios na amostra estudada foi alta. As crianças que usavam chupeta por mais de três anos mostraram maior probabilidade de apresentar mordida aberta.718

Medullary control of nociceptive transmission: reciprocal dual communication with the spinal cord

Control of pain perception, essential for organism surviving and recovery from disease, is exerted by higher brain centers integrating nociception with emotional and cognitive information and modulating the brainstem-spinal feedback loops that regulate spinal nociceptive transmission. Development of chronic pain deregulates the forebrain-brainstem-spinal pain control system, which leads to neuroplasticity and disruption of a balanced brain-spinal communication. Targets for impeding pain chronification are being developed using the manipulation of the cross talk between brain and dorsal horn, at both sites of the loop.FCT -Fuel Cell Technologies Program(POCTI/NSE/46399/2002

Polymorphisms in the BER and NER pathways and their influence on survival and toxicity in never-smokers with lung cancer

Polymorphisms in DNA repair pathways may play a relevant role in lung cancer survival in never-smokers. Furthermore, they could be implicated in the response to chemotherapy and toxicity of platinum agents. The aim of this study was to evaluate the influence of various genetic polymorphisms in the BER and NER DNA repair pathways on survival and toxicity in never-smoker LC patients. The study included never-smokers LC cases diagnosed from 2011 through 2019, belonging to the Lung Cancer Research In Never Smokers study. A total of 356 never-smokers cases participated (79% women; 83% adenocarcinoma and 65% stage IV). Survival at 3 and 5 years from diagnosis was not associated with genetic polymorphisms, except in the subgroup of patients who received radiotherapy or chemo-radiotherapy, and presented with ERCC1 rs3212986 polymorphism. There was greater toxicity in those presenting OGG1 rs1052133 (CG) and ERCC1 rs11615 polymorphisms among patients treated with radiotherapy or chemo-radiotherapy, respectively. In general, polymorphisms in the BER and NER pathways do not seem to play a relevant role in survival and response to treatment among never-smoker LC patients

A snapshot of cancer-associated thromboembolic disease in 2018-2019: First data from the TESEO prospective registry

BACKGROUND: The ever-growing complexity of cancer-associated thrombosis (CAT), with new antineoplastic drugs and anticoagulants, distinctive characteristics, and decisions with low levels of evidence, justifies this registry. METHOD: TESEO is a prospective registry promoted by the Spanish Society of Medical Oncology to which 34 centers contribute cases. It seeks to provide an epidemiological description of CAT in Spain. RESULTS: Participants (N=939) with CAT diagnosed between July 2018 and December 2019 were recruited. Most subjects had advanced colon (21.4%), non-small cell lung (19.2%), and breast (11.1%) cancers, treated with dual-agent chemotherapy (28.4%), monochemotherapy (14.4%), or immune checkpoint inhibitors (3.6%). Half (51%) were unsuspected events, albeit only 57.1% were truly asymptomatic. Pulmonary embolism (PE) was recorded in 571 (58.3%); in 120/571 (21.0%), there was a concurrent deep venous thromboembolism (VTE). Most initially received low molecular weight heparin (89.7%). Suspected and unsuspected VTE had an OS rate of 9.9 (95% CI, 7.3-non-computable) and 14.4 months (95% CI, 12.6-non-computable) (p=0.00038). Six-month survival was 80.9%, 55.9%, and 55.5% for unsuspected PE, unsuspected PE admitted for another reason, and suspected PE, respectively (p<0.0001). The 12-month cumulative incidence of venous rethrombosis was 7.1% (95% CI, 4.7-10.2) in stage IV vs 3.0% (95% CI, 0.9-7.1) in stages I-III. The 12-month cumulative incidence of major/clinically relevant bleeding was 9.6% (95% CI, 6.1-14.0) in the presence of risk factors. CONCLUSION: CAT continues to be a relevant problem in the era of immunotherapy and targeted therapies. The initial TESEO data highlight the evolution of CAT, with new agents and thrombotic risk factors