Use of Chemical Modification To Elucidate RNA Folding Pathways

As discussed in this overview, chemical modification is sensitive to the accessibility of a nucleotide to the solvent, and many nucleotides become less accessible as an RNA folds into its structured form. Chemical modification reagents are therefore suitable for following RNA folding, and can be used to study the kinetics of structure formation on time scales ranging from minutes to hours.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/143596/1/cpnc1109.pd

The impact of Arctic warming on the midlatitude jetstream: Can it? Has it? Will it?

Copyright © 2015 John Wiley & Sons, LtdThe Arctic lower atmosphere has warmed more rapidly than that of the globe as a whole, and this has been accompanied by unprecedented sea ice melt. Such large environmental changes are already having profound impacts on the flora, fauna, and inhabitants of the Arctic region. An open question, however, is whether these Arctic changes have an effect on the jet-stream and thereby influence weather patterns farther south. This broad question has recently received a lot of scientific and media attention, but conclusions appear contradictory rather than consensual. We argue that one point of confusion has arisen due to ambiguities in the exact question being posed. In this study, we frame our inquiries around three distinct questions: Can Arctic warming influence the midlatitude jet-stream? Has Arctic warming significantly influenced the midlatitude jet-stream? Will Arctic warming significantly influence the midlatitude jet-stream? We argue that framing the discussion around the three questions: Can it?, Has it?, and Will it? provides insight into the common themes emerging in the literature as well as highlights the challenges ahead

Ir(III) complexes of diamine ligands for asymmetric ketone hydrogenation

The use of a combination of IrCl3 with a series of ligands derived from the C2-symmetric diamine diphenylethanediamine (DPEN) forms a catalyst capable of the asymmetric hydrogenation of ketones in up to 85% ee

Phosphine-free chiral iridium catalysts for asymmetric catalytic hydrogenation of simple ketones

Novel pyridylalkylamine and aminopyridinato ligand stabilized iridium complexes with no P ligand are introduced. These complexes have been investigated as catalysts for asymmetric hydrogenation of simple ketones, resulting in an active catalyst for bulky alkyl aryl ketones that is α-methylpropiophenone. The ligands were synthesized from inexpensive starting materials and their modular design allows for the introduction of a broad variety of substitution patterns. Additionally, better activity and selectivity was observed at 20 °C and 20 bar H2 pressure with a catalyst loading as low as 0.05 mol% iridium. These phosphorus free catalysts have always been a central issue in both academic and industrial research

Primary histologic diagnosis using automated whole slide imaging: a validation study

BACKGROUND: Only prototypes 5 years ago, high-speed, automated whole slide imaging (WSI) systems (also called digital slide systems, virtual microscopes or wide field imagers) are becoming increasingly capable and robust. Modern devices can capture a slide in 5 minutes at spatial sampling periods of less than 0.5 micron/pixel. The capacity to rapidly digitize large numbers of slides should eventually have a profound, positive impact on pathology. It is important, however, that pathologists validate these systems during development, not only to identify their limitations but to guide their evolution. METHODS: Three pathologists fully signed out 25 cases representing 31 parts. The laboratory information system was used to simulate real-world sign-out conditions including entering a full diagnostic field and comment (when appropriate) and ordering special stains and recuts. For each case, discrepancies between diagnoses were documented by committee and a "consensus" report was formed and then compared with the microscope-based, sign-out report from the clinical archive. RESULTS: In 17 of 25 cases there were no discrepancies between the individual study pathologist reports. In 8 of the remaining cases, there were 12 discrepancies, including 3 in which image quality could be at least partially implicated. When the WSI consensus diagnoses were compared with the original sign-out diagnoses, no significant discrepancies were found. Full text of the pathologist reports, the WSI consensus diagnoses, and the original sign-out diagnoses are available as an attachment to this publication. CONCLUSION: The results indicated that the image information contained in current whole slide images is sufficient for pathologists to make reliable diagnostic decisions and compose complex diagnostic reports. This is a very positive result; however, this does not mean that WSI is as good as a microscope. Virtually every slide had focal areas in which image quality (focus and dynamic range) was less than perfect. In some cases, there was evidence of over-compression and regions made "soft" by less than perfect focus. We expect systems will continue to get better, image quality and speed will continue to improve, but that further validation studies will be needed to guide development of this promising technology