免疫組織染色法によるヒストンH3リジン9の修飾とメチル化酵素の検出および骨格系細胞分化過程におけるそれらの局在変化

早大学位記番号:新6540早稲田大

Immunohistochemical detection of methylated histone H3 lysine 9 and histone methyltransferases and implication of their roles during skeletal cell differentiation

早大学位記番号:新6540博士(人間科学)早稲田大

潜在的連想テスト(Implicit Association Test)のリスク認知への応用

早大学位記番号:新7568早稲田大

Prevalence of Exogenous Hormone Use in Japanese Women

Background: There have been few community-based epidemiological studies in which the prevalence of exogenous hormone use, including the use of oral contraceptives (OCs) and hormone replacement therapy (HRT), has been accurately assessed in Japan. Methods: We have been conducting repeated surveys of participants in the Japan Nurses’ Health Study (JNHS), as a nationwide prospective cohort study, since 2001. We determined the prevalence of exogenous hormone use at baseline and during a 10-year follow-up period. A total of 15,019 female nurses participated in the JNHS follow-up cohort. We determined the prevalence of OC use in 14,839 women <60 years of age at baseline and the prevalence of HRT use in 7,915 women, excluding premenopausal women, at the last time they answered a questionnaire. The duration of HRT use was estimated using the Kaplan-Meier method. Results: Six percent of the participants used OCs. The proportion of HRT users who stopped HRT before the baseline survey, the proportion of women using HRT during the follow-up period, and the proportion of all of the participants who had used HRT were 3.2%, 10.6%, and 13.8%, respectively. The median duration of HRT use was 2 years. Conclusions: The lifetime prevalences of exogenous hormone use during this prospective study conducted in Japanese nurses were 6.0% for OCs and 13.8% for HRT. The information obtained in this study will be useful for clarification of the association between exogenous estrogen exposure and estrogen-related diseases as future research

Urinary FSH in postmenopausal women

The change in follicle-stimulating hormone (FSH) during the menopausal transition and associations of FSH with various diseases have been assessed by using blood samples. We examined cross-sectionally the variation of FSH levels, associations of estrone and estradiol with FSH, and associations of BMI with these hormones by using urinary samples from peri- and postmenopausal women in Japan. Of 4472 participants in the Urinary Isoflavone Concentration Survey of the Japan Nurses’ Health Study, we analyzed urinary levels of estrone, estradiol and FSH in 547 women aged from 45 to 54 years. Urinary FSH levels varied widely in postmenopausal women and the pattern of change in urinary FSH levels seems to be similar to that in blood FSH levels in previous studies. There were no significant differences in age, body mass index (BMI), estradiol, estrone and estradiol/estrone ratio among three groups according to the tertile of FSH. In postmenopausal women, there were significant associations of BMI with levels of estrone and estradiol, but there was no significant association of BMI with FSH. Studies using urinary samples will allow us to establish a study project as a large-scale population-based study to determine associations between FSH and various diseases after menopause

A Case Study Considering “Food-Related Education” in Combination with Environment to Foster Ordinary Food Habits of Children (2) : Situations about the Practice of Food-Related Education in China, and Possibilities of Focusing on the Concept “Culture”

　学校教育における今日的課題として，学校・家庭・地域相互の連携を目指した，子どもの食習慣を確立させるための食教育実践の充実が求められている。先んじて，第Ⅰ報では，これまでの食教育実践に関する先行研究の現状を，子どもの食教育に関する基本的価値認識の観点から明らかにしていった。それに続く第Ⅱ報では，食教育の比較教育学研究を試みるため，第Ⅰ報での研究視点・研究方法を援用しながら，中国における食教育実践に関する先行研究の現状について検討し，日中両国の食教育の類似点・相違点を明らかにしていくことで，「文化」概念を中核に据えた就学前・初等教育における食教育実践の検討可能性を理論的に示唆することを試みていく

A Case Study Considering “Food-Related Education” in Combination with Environment to Foster Ordinary Food Habits of Children (1) : Situations about the Practice of Food-Related Education in Japan from the Viewpoints of Recognizing Common Basic Values

　学校教育における今日的課題として，学校・家庭・地域相互の連携を目指した，子どもの食習慣を確立させるための食教育1）実践の充実が求められている。本研究では，これまでの食教育の理論と実践に関する先行研究の特質と課題を見出し，「学習者と人との関わり」を重視した食教育として，文化概念を中核に据えた就学前・初等教育における食教育の試みを理論的・実証的に考察することを目的とする。このうち，第Ⅰ報では，子どもの食教育に関する基本的価値認識の観点から，これまでにわが国でなされている食教育実践に関する先行研究の現状を明らかにしていく

Association between FSH, E1, and E2 levels in urine and serum in premenopausal and postmenopausal women

Objective: We aimed to establish correlations for the levels of follicle-stimulating hormone (FSH), estrone (E1) and estradiol (E2) between urine and serum in premenopausal and postmenopausal women using immunoassays. Methods: In this study of 92 women (61 postmenopausal, 31 premenopausal), both urine and blood specimens were collected on the same day and stored at 4 °C for analysis by chemiluminescent immunoassay, radioimmunoassay and/or electrochemiluminescent immunoassay. Results: There were correlations in the levels of FSH, E1 and E2 between urine and serum in both postmenopausal (r = 0.96 for FSH, r = 0.91 for E1, r = 0.80 for E2) and premenopausal (r = 0.98 for FSH, r = 0.92 for E1, r = 0.90 for E2) women. It is indicated that the correlations were stronger in the premenopausal group compared with the postmenopausal group, especially for FSH. Conclusion: The levels of FSH, E1 and E2 in urine correlated with those in the serum in premenopausal and postmenopausal women. Urine samples could be used instead of serum samples to measure hormone levels, which would reduce the difficulty of conducting large survey studies