Shining light on location-biased cAMP signaling

cAMP is the indispensable second messenger regulating cell metabolism and function in response to extracellular hormones and neurotransmitters. cAMP is produced via the activation of G protein–coupled receptors located at both the cell surface and inside the cell. Recently, Tsvetanova et al. explored cAMP generation in distinct locations and the impact on respective cell functions. Using a phospho-proteomic analysis, they provide insight into the unique role of localized cAMP production in cellular phospho-responses

Second generation of Fucose-based DC-SIGN ligands : affinity improvement and specificity versus Langerin

DC-SIGN and Langerin are two C-type lectins involved in the initial steps of HIV infections: the former acts as a viral attachment factor and facilitates viral invasion of the immune system, the latter has a protective effect. Potential antiviral compounds targeted against DC-SIGN were synthesized using a common fucosylamide anchor. Their DC-SIGN affinity was tested by SPR and found to be similar to that of the natural ligand Lewis-X (Le X). The compounds were also found to be selective for DC-SIGN and to interact only weakly with Langerin. These molecules are potentially useful therapeutic tools against sexually transmitted HIV infection

Synthesis of a selective inhibitor of a fucose binding bacterial lectin from <i>Burkholderia ambifaria</i>

International audienc

Synthesis of inhibitors of DC-SIGN mediated infections

HIV infection is pandemic in humans and is responsible for millions of deaths every year. The discovery of new cellular targets that can be used to prevent the infection process represents a new opportunity for developing more effective antiviral drugs. On the poster, dendritic cell-specific ICAM-3 grabbing non-integrin (DC-SIGN), a lectin expressed at the surface of immature dendritic cells and involved in the initial stages of HIV infection, is described as a promising therapeutic target. The project is being developed within the European research Network CARMUSYS (http://www.carmusys.iiq.csic.es/). Herein we show the synthesis of a small library of derivatives of a dimannoside mimic recently reported by our laboratory.1 The mimic was functionalized with two identical amide groups, and a tetravalent presentation was obtained using a dendron as the polyvalent scaffold. The prepared amides exhibit DC-SIGN inhibition in micromolar range, what was confirmed by measurements using surface plasma resonance (SPR) technique

Synthesis of inhibitors of DC-SIGN mediated infections

HIV infection is pandemic in humans and is responsible for millions of deaths every year. The discovery of new cellular targets that can be used to prevent the infection process represents a new opportunity for developing more effective antiviral drugs. In this work, dendritic cell-specific ICAM-3 grabbing non-integrin (DC-SIGN), a lectin expressed at the surface of immature dendritic cells and involved in the initial stages of HIV infection, is described as a promising therapeutic target. The project is being developed within the European research Network CARMUSYS (http://www.carmusys.iiq.csic.es). Herein we show the synthesis of a small library of derivatives of a dimannoside mimic recently reported by our laboratory.1 The mimic was functionalized with two identical amide groups. Further, multivalent presentations of the prepared DC-SIGN ligands were obtained via click chemistry using dendrimeric scaffolds. The activities of the prepared molecules towards DC-SIGN were determined using surface plasmon resonance (SPR) technique. Multivalency showed significant improvement of the DC-SIGN inhibition in comparison with the corresponding monovalent ligands

Synthesis of a selective inhibitor of a fucose binding bacterial lectin from <i>Burkholderia ambifaria</i>

International audienc

Zur Technik der heimlichen Online-Durchsuchung

Zur Durchführung der so genannten (heimlichen) Online-Durchsuchung existiert eine ganze Reihe falscher Vermutungen wie universell einsetzbare Würmer oder Viren, die der (ebenfalls falsche) Begriff 'Bundestrojaner' suggeriert. Im Beitrag wird das seit drei Jahren von den deutschen Behörden praktizierte Verfahren zur Online-Durchsuchung vorgestellt: Less-Than-Zero-Day-Exploits. Es handelt sich dabei um bestimmte Angriffsprogramme, die den Software-Herstellern noch nicht bekannte Sicherheitslücken in ihren Systemen ausnutzen. Bei einer Online-Durchsuchung wird in erster Linie versucht, eine Kopie des Inhaltsverzeichnisses von Datenträgern zu erhalten und die Relevanz der dort gespeicherten Daten zu bewerten