Tidal controls on trace gas dynamics in a seagrass meadow of the Ria Formosa lagoon (southern Portugal)

Coastal zones are important source regions for a variety of trace gases, including halocarbons and sulfur-bearing species. While salt marshes, macroalgae and phyto-plankton communities have been intensively studied, little is known about trace gas fluxes in seagrass meadows. Here we report results of a newly developed dynamic flux chamber system that can be deployed in intertidal areas over full tidal cycles allowing for highly time-resolved measurements. The fluxes of CO2, methane (CH4) and a range of volatile organic compounds (VOCs) showed a complex dynamic mediated by tide and light. In contrast to most previous studies, our data indicate significantly enhanced fluxes during tidal immersion relative to periods of air exposure. Short emission peaks occurred with onset of the feeder current at the sampling site. We suggest an overall strong effect of advective transport processes to explain the elevated fluxes during tidal immersion. Many emission estimates from tidally influenced coastal areas still rely on measurements carried out during low tide only. Hence, our results may have significant implications for budgeting trace gases in coastal areas. This dynamic flux chamber system provides intensive time series data of community respiration (at night) and net community production (during the day) of shallow coastal systems.German Federal Ministry of Education and Research (BMBF) [03F0611E, 03F0662E]; EU FP7 ASSEMBLE research infrastructure initiative

A high volume sampling system for isotope determination of volatile halocarbons and hydrocarbons

The isotopic composition of volatile organic compounds (VOCs) can provide valuable information on their sources and fate not deducible from mixing ratios alone. In particular the reported carbon stable isotope ratios of chloromethane and bromomethane from different sources cover a δ13C-range of almost 100‰ making isotope ratios a very promising tool for studying the biogeochemistry of these compounds. So far, the determination of the isotopic composition of C1 and C2 halocarbons others than chloromethane is hampered by their low mixing ratios. In order to determine the carbon isotopic composition of C1 and C2 halocarbons with mixing ratios as low as 1 pptv (i) a field suitable cryogenic high volume sampling system and (ii) a chromatographic set up for processing these samples have been developed and validated. The sampling system was tested at two different sampling sites, an urban and a coastal location in Northern Germany. The average δ13C-values for bromomethane at the urban site were −42.9 ± 1.1‰ and agreed well with previously published results. But at the coastal site bromomethane was substantially enriched in 13C by almost 10‰. Less pronounced differences were observed for chlorodifluoromethane, 1,1,1-trichloroethane and chloromethane. We suggest that these differences are related to the turnover of these compounds in ocean surface waters. Furthermore we report first carbon isotope ratios for iodomethane (−40.4‰ to −79.8‰), bromoform (−13.8‰ to 22.9‰), and other halocarbons

Quasiparticle Chirality in Epitaxial Graphene Probed at the Nanometer Scale

Graphene exhibits unconventional two-dimensional electronic properties resulting from the symmetry of its quasiparticles, which leads to the concepts of pseudospin and electronic chirality. Here we report that scanning tunneling microscopy can be used to probe these unique symmetry properties at the nanometer scale. They are reflected in the quantum interference pattern resulting from elastic scattering off impurities, and they can be directly read from its fast Fourier transform. Our data, complemented by theoretical calculations, demonstrate that the pseudospin and the electronic chirality in epitaxial graphene on SiC(0001) correspond to the ones predicted for ideal graphene.Comment: 4 pages, 3 figures, minor change

A halocarbon survey from a seagrass dominated subtropical lagoon, Ria Formosa (Portugal): flux pattern and isotopic composition

In this study we report fluxes of chloromethane (CH3Cl), bromomethane (CH3Br), iodomethane (CH3I), and bromoform (CHBr3) from two sampling campaigns (summer and spring) in the seagrass dominated subtropical lagoon Ria Formosa, Portugal. Dynamic flux chamber measurements were performed when seagrass patches were either air-exposed or submerged. Overall, we observed highly variable fluxes from the seagrass meadows and attributed them to diurnal cycles, tidal effects, and the variety of possible sources and sinks in the seagrass meadows. The highest emissions with up to 130 nmol m−2 h−1 for CH3Br were observed during tidal changes, from air exposure to submergence and conversely. Furthermore, during the spring campaign, the emissions of halocarbons were significantly elevated during tidal inundation as compared to air exposure. Accompanying water sampling performed during both campaigns revealed elevated concentrations of CH3Cl and CH3Br, indicating productive sources within the lagoon. Stable carbon isotopes of halocarbons from the air and water phase along with source signatures were used to allocate the distinctive sources and sinks in the lagoon. Results suggest that CH3Cl was rather originating from seagrass meadows and water column than from salt marshes. Aqueous and atmospheric CH3Br was substantially enriched in 13C in comparison to source signatures for seagrass meadows and salt marshes. This suggests a significant contribution from the water phase on the atmospheric CH3Br in the lagoon. A rough global upscaling yields annual productions from seagrass meadows of 2.3–4.5 Gg yr−1, 0.5–1.0 Gg yr−1, 0.6–1.2 Gg yr−1, and 1.9–3.7 Gg yr−1 for CH3Cl, CH3Br, CH3I, and CHBr3 respectively. This suggests a minor contribution from seagrass meadows to the global production of CH3Cl and CH3Br with about 0.1 and 0.7%, respectively. In comparison to the known marine sources for CH3I and CHBr3, seagrass meadows are rather small sources

References

www.biogeosciences-discuss.net/11/10605/2014/ doi:10.5194/bgd-11-10605-2014 © Author(s) 2014. CC Attribution 3.0 License. This discussion paper is/has been under review for the journal Biogeosciences (BG). Please refer to the corresponding final paper in BG if available. A halocarbon survey from a seagrass dominated subtropical lagoon, Ria Formosa (Portugal): flux pattern and isotopic compositio

Statistical properties of multistep enzyme-mediated reactions

Enzyme-mediated reactions may proceed through multiple intermediate conformational states before creating a final product molecule, and one often wishes to identify such intermediate structures from observations of the product creation. In this paper, we address this problem by solving the chemical master equations for various enzymatic reactions. We devise a perturbation theory analogous to that used in quantum mechanics that allows us to determine the first () and the second (variance) cumulants of the distribution of created product molecules as a function of the substrate concentration and the kinetic rates of the intermediate processes. The mean product flux V=d/dt (or "dose-response" curve) and the Fano factor F=variance/ are both realistically measurable quantities, and while the mean flux can often appear the same for different reaction types, the Fano factor can be quite different. This suggests both qualitative and quantitative ways to discriminate between different reaction schemes, and we explore this possibility in the context of four sample multistep enzymatic reactions. We argue that measuring both the mean flux and the Fano factor can not only discriminate between reaction types, but can also provide some detailed information about the internal, unobserved kinetic rates, and this can be done without measuring single-molecule transition events.Comment: 8 pages, 3 figure

Geomagnetic data from the GOCE satellite mission

The Gravity field and steady-state Ocean Circulation Explorer (GOCE) is part of ESA’s Earth Explorer Program. The satellite carries magnetometers that control the activity of magnetorquers for navigation of the satellite, but are not dedicated as science instruments. However, intrinsic steady states of the instruments can be corrected by alignment and calibration, and artificial perturbations, e.g. from currents, can be removed by their characterisation correlated to housekeeping data. The leftover field then shows the natural evolution and variability of the Earth’s magnetic field. This article describes the pre-processing of input data as well as calibration and characterisation steps performed on GOCE magnetic data, using a high-precision magnetic field model as reference. For geomagnetic quiet times, the standard deviation of the residual is below 13 nT with a median residual of (11.7, 9.6, 10.4) nT for the three magnetic field components (x, y, z). For validation of the calibration and characterisation performance, we selected a geomagnetic storm event in March 2013. GOCE magnetic field data show good agreement with results from a ground magnetic observation network. The GOCE mission overlaps with the dedicated magnetic field satellite mission CHAMP for a short time at the beginning of 2010, but does not overlap with the Swarm mission or any other mission flying at low altitude and carrying high-precision magnetometers. We expect calibrated GOCE magnetic field data to be useful for lithospheric modelling and filling the gap between the dedicated geomagnetic missions CHAMP and Swarm. Graphic Abstract: [Figure not available: see fulltext.]

Alternating hemiplegia of childhood: First South African case report and verapamil as a possible treatment option

In this case report we describe a child with alternating hemiplegia of childhood, a rare neurodevelopmental disorder, and the effectiveness of an unconventional drug, verapamil, in treating this condition

Field-aligned current associated with low-latitude plasma blobs as observed by the CHAMP satellite

Here we give two examples of low-latitude plasma blobs accompanied by linearly polarized perpendicular magnetic deflections which imply that associated field-aligned currents (FACs) have a 2-D sheet structure located at the blob walls. The estimated FAC density is of the order of 0.1 μA/m<sup>2</sup>. The direction of magnetic deflections points westward of the magnetic meridian and there is a linear correlation between perpendicular and parallel variations. All these properties are similar to those of equatorial plasma bubbles (EPBs). According to CHAMP observations from August 2000 to July 2004, blobs show except for these two good examples no clear signatures of 2-D FAC sheets at the walls. Generally, perpendicular magnetic deflections inside blobs are weaker than inside EPBs on average. Our results are consistent with existing theories: if a blob exists, (1) a significant part of EPB FAC will be closed through it, exhibiting similar perpendicular magnetic deflection inside EPBs and blobs, (2) the FAC closure through blobs leads to smaller perpendicular magnetic deflection at its poleward/downward side, and (3) superposition of different FAC elements might result in a complex magnetic signature around blobs