LiDAR-based digital outcrops for sedimentological analysis: workflows and techniques

Recent developments in workflows and techniques for the integration and analysis of terrestrial LiDAR (Light Detection And Ranging) and conventional outcrop datasets are demonstrated through three case studies. The first study shows the power of three-dimensional (3D) data visualization, in association with an innovative surface-modelling technique, for establishing large-scale 3D stratigraphical frameworks. The second presents an approach to derive reliable geometrical data on sediment-body geometries, whereas the third presents a new technique to quantify the proportions, distributions and variability of sedimentary facies directly from outcrop. In combination, these techniques provide essential conditioning data for geocellular and stochastic facies modelling. Built upon robust, reproducible and quantitative data, the resultant models combine realistic 3D geological architectures with sufficient quantities of reliable numerical data required for stable statistical analysis and establishing uncertainty. Together this new information provides detailed understanding and quantification of the 3D complexity of the sedimentary systems in question, thus offering insights of value for predicting the subsurface anatomy of analogous petroleum systems. As such, use of LiDAR, when combined with conventional field geology, offers a powerful tool for quantitative outcrop analysis, tightly constraining 3D structural and stratigraphical interpretations, and effectively increasing the statistical significance of outcrop analogues for reservoir characterization.F. Rarity, X. M. T. Van Lanen, D. Hodgetts, R. L. Gawthorpe, P. Wilson, I. Fabuel-Perez, & J. Redfer

An integrated study of Permo-Triassic basins along the North Atlantic passive margin: implication for future exploration

Permo-Triassic rift basins offer important hydrocarbon targets along the Atlantic margins. Their fill is dominated by continental red beds, comprising braided fluvial, alluvial fan, aeolian, floodplain and lacustrine facies. These relatively lightly explored basins span both the Atlantic and Tethyan domains and developed above a complex basement with inherited structural fabrics. Sparse data in offshore regions constrain understanding of depositional geometries and sedimentary architecture, further impeded by their deep burial beneath younger strata, combined with the effects of later deformation during continental breakup. This paper provides results from a multidisciplinary analysis of basins along the Atlantic margin. Regional seismic and well data, combined with geochemical provenance analysis from the European North Atlantic margins, are integrated with detailed outcrop studies in Morocco and Nova Scotia. The research provides new insights into regional basin tectonostratigraphic evolution, sediment fill, and reservoir distribution, architecture and quality at a range of scales. Regional seismic profiles, supported by key well data, indicate the presence of post-orogenic collapse basins, focused narrow rifts and low-magnitude multiple extensional depocentres. Significantly, Permo-Triassic basin geometries are different and more varied than the overlying Jurassic and younger basins. Provenance analysis using Pb isotopic composition of detrital K-feldspar yields new and robust controls on the sediment dispersal patterns of Triassic sandstones in the NE Atlantic margin. The evolving sedimentary architecture is characterized by detailed sedimentological studies of key outcrops of age equivalent Permian-Triassic rifts in Morocco and Nova Scotia. The interplay of tectonics and climate is observed to influence sedimentation, which has significant implications for reservoir distribution in analogue basins. New digital outcrop techniques are providing improved reservoir models, and identification of key marker horizons and sequence boundaries offers a potential subsurface correlation tool. Future work will address source and seal distribution within the potentially petroliferous basins

Somatic embryogenesis from seeds in a broad range of Vitis vinifera L. varieties: rescue of true-to-type virus-free plants

Abstract Background Somatic embryogenesis is the preferred method for cell to plant regeneration in Vitis vinifera L. However, low frequencies of plant embryo conversion are commonly found. In a previous work we obtained from cut-seeds of a grapevine infected with the Grapevine leafroll associated viruses 1 and 3 (GLRaV-1 and GLRaV-3), high rates of direct regeneration, embryo plant conversion and sanitation. The aim of this study is to evaluate the usefulness of this procedure for regeneration of other grapevine varieties which include some infected with one to three common grapevine viruses (GLRaV-3, Grapevine fanleaf virus (GFLV) and Grapevine fleck virus (GFkV)). As grapevine is highly heterozygous, it was necessary to select from among the virus-free plants those that regenerated from mother tissues around the embryo, (true-to-type). Results Somatic embryogenesis and plant regeneration were achieved in a first experiment, using cut-seeds from the 14 grapevine varieties Airén, Cabernet Franc, Cabernet Sauvignon, Mencía, Merlot, Monastrell, Petit Verdot, Pinot Blanc (infected by GFLV and GFkV), Pinot Gris, Pinot Meunier, Pinot Noir, Syrah, Tempranillo (infected by GFLV), and Verdil. All regenerated plants were confirmed to be free of GFkV whereas at least 68% sanitation was obtained for GFLV. The SSR profiles of the virus-free plants showed, in both varieties, around 10% regeneration from mother tissue (the same genetic make-up as the mother plant). In a second experiment, this procedure was used to sanitize the varieties Cabernet Franc, Godello, Merlot and Valencí Blanc infected by GLRaV-3, GFkV and/or GFLV. Conclusions Cut-seeds can be used as explants for embryogenesis induction and plant conversion in a broad range of grapevine varieties. The high regeneration rates obtained with this procedure facilitate the posterior selection of true-to-type virus-free plants. A sanitation rate of 100% was obtained for GFkV as this virus is not seed-transmitted. However, the presence of GLRaV-3 and GFLV in some of the regenerated plants showed that both viruses are seed-transmitted. The regeneration of true-to-type virus-free plants from all infected varieties indicates that this methodology may represent an alternative procedure for virus cleaning in grapevine