UAS Literary & Arts Journal

Proof copy provided by Tidal Echoes.The 2015 edition of Tidal Echoes presents an annual showcase of writers and artists who share one thing in common: a life surrounded by the rainforests and waterways of Southeast Alaska.Dedication -- Editor’s Note -- Mid-Air -- Acknowledgements -- Salmon (Gohan Desu Yo/It’s Dinner Time) -- Xwaayeenák Richard Dauenhauer -- Loon -- Burney Falls -- Richard Dauenhauer -- Egg Carton Fox -- August Afternoon at Helle’s Pool, Vancouver, Washington -- Oak Run -- Buddy Tabor teaches me to filet a halibut -- Icelander -- Home -- Season’s End -- Young Me, Old Me -- The Window Seat -- Wind -- UAS Student Back Study -- Paintbrush Conversations -- Grey River Soulshine -- Across the Universe -- Aurora-Skaters’ Cabin 1 -- Whiskey and Autumn -- Leaf Wolf -- Spring Cleaning the Perennials -- Ode to a Rose -- Final Point -- After Spring Recital -- Olympic Ceiling -- Rodda-Hard going (too little snow) -- Birch Bark Calligraphy No. 2 -- Fishtailing -- Working the Corks -- Modern Alaskan Storyteller: An Interview with Ishmael Hope -- Bailer at the Back of the Boat (Excerpt) -- Close Up -- Bothering the Dauenhauers -- In Memory of Andrew Hope III -- Wolf Brimhat -- I Am From -- Crossing -- Revelations and Realizations -- The Shrinking Girl -- Untitled (Andi in Niki’s Room) -- Afternoon Reading, Rainy Room -- My Grandmother’s House in Metlakatla -- Deacon Charles Rohrbacher, Icon of St. Nicholas -- Honoring Tibet -- Fumi Matsumoto: Finding Art in Culture -- Pathway of Thorns -- Minidoka Interlude -- Mountain Dew Parrots -- Watch Out for Falling Objects -- Loose Change -- An Unkind Demise -- A Place That Holds Names -- Immortality (skull side) -- Opening Again the Box of Wisdom -- Iff’n I Go -- Colorless Blues -- The Heartsdance -- King of Dreams -- Hungering -- Colonialism -- Inside Out -- Rachel Day -- Lying Here -- Note to Wife -- Death -- Round the Clock -- Untitled (trap) -- Seeds of Racism -- Auschwitz Remembrance -- Moab 1 -- Pearl of the Orient -- Moab 3 -- Scars -- Transporting -- In Eliason Harbor -- Poet Passes: Leaves Words Behind -- Tough Guy -- Wolf Helmet -- Biographie

UAS Literary & Arts Journal

Proof copy provided by Tidal Echoes.The 2016 edition of Tidal Echoes presents an annual showcase of writers and artists who share one thing in common: a life surrounded by the rainforests and waterways of Southeast Alaska.a heart is a heavy burden -- Editor’s Note -- Acknowledgements -- Always a Pause -- Untitled -- Mishima Teabowls -- Mint Tea and Red Wine -- The Dry Winter Scent of Prairies -- Northern Sunrise -- Musical Theory in a Falling Tree -- Jumbo Lookout -- The Shape of an Echo -- Wonderwall -- It’s Difficult [excerpt from the poem “Marissa”] -- Yéil x’us.eetí Raven Footprints -- táakw winter -- King Fisher -- Bear -- A Dandelion by its own Name -- Migrations -- Untitled -- They Named Her Driftwood -- Kissing in the Rain -- Waltz of the Flowers: Anna and Company -- Sun Catcher -- Porcelain Curtains -- The River -- Kathleen Lake -- A Living Tapestry -- Red Cedar Tlingit Haida Weave -- Skinned -- Survivor -- Auke Lake Lights -- Aurora Chasers -- Emma Afloat -- I Can’t Sleep -- The Ghost I’m Left With -- Framed Memories -- Tequila, Sweat, and Prayers -- The Alchemical Marriage -- A New New Hope -- each dream practice -- Clouds -- Seasons Change and the Waters Run On -- John Muir -- Salmon Speaks -- A Place That Holds Names -- Untitled -- Salmon Spirit Chest, Connected (box) -- The Language of Weaving: Featured Artist Dr. Teri Rofkar -- Nome, Alaska -- Drained -- I’m From -- Srevlla—The state of things where the spring snow is so soft that one sinks into it. -- Reflections -- Txamsem -- Post-Glacial Rebound -- Dressed in Garlands -- Learning the Dance -- Shamrocks -- When You See Me -- Water Spirit -- To Speak for Restraint, for Wildness, for Beauty: Featured Writer Aleria Jensen -- Young Eagle -- Untitled (detail) -- Baula -- Airport Dike Trail Evening -- Ninja Meatballs -- Untitled -- In the Wild Without Child: One Mother’s Invitation to Self -- Whale Tail Vista -- Questions for Anemones -- Yellow Cedarbark Wool on Starfish Pot -- Gleaming Orange and Pissed -- Through the Sky She Comes -- Love for the Honeybee -- The Last Speaker -- Juneau Fireweed -- Judy Plays the Tuba -- Illuminated Juneau -- Airport Dike Trail Moonlight -- Escape -- A Wooden Mother -- Black and Whites -- an excuse for staying indoors (a working title for a work in progress) -- Insecurities -- Lake Farm -- Yew Bear -- Rupture -- Mendenhall Glacier -- The Rookery -- Transient -- Laminaria -- Untitled -- Querencia -- Hoard -- Untitled -- Small Birds Sign -- Selfie at Two -- Entropy -- Goodbye -- Sitka Bridge -- My Remedy -- S1 -- Delta Symbols Static -- Falling -- Breathe Deeply -- Drum, drum away -- Eaglefest Dancer -- Wasichana -- Writer & Artist Biographie

Truncating the Y-Axis: Threat or Menace?

Bar charts with y-axes that don't begin at zero can visually exaggerate effect sizes. However, advice for whether or not to truncate the y-axis can be equivocal for other visualization types. In this paper we present examples of visualizations where this y-axis truncation can be beneficial as well as harmful, depending on the communicative and analytic intent. We also present the results of a series of crowd-sourced experiments in which we examine how y-axis truncation impacts subjective effect size across visualization types, and we explore alternative designs that more directly alert viewers to this truncation. We find that the subjective impact of axis truncation is persistent across visualizations designs, even for designs with explicit visual cues that indicate truncation has taken place. We suggest that designers consider the scale of the meaningful effect sizes and variation they intend to communicate, regardless of the visual encoding

Bio-Sensing of Cadmium(II) Ions Using Staphylococcus aureus†

Cadmium, as a hazardous pollutant commonly present in the living environment, represents an important risk to human health due to its undesirable effects (oxidative stress, changes in activities of many enzymes, interactions with biomolecules including DNA and RNA) and consequent potential risk, making its detection very important. New and unique technological and biotechnological approaches for solving this problems are intensely sought. In this study, we used the commonly occurring potential pathogenic microorganism Staphylococcus aureus for the determination of markers which could be used for sensing of cadmium(II) ions. We were focused on monitoring the effects of different cadmium(II) ion concentrations (0, 1.25, 2.5, 5, 10, 15, 25 and 50 μg mL−1) on the growth and energetic metabolism of Staphylococcus aureus. Highly significant changes have been detected in the metabolism of thiol compounds—specifically the protein metallothionein (0.79–26.82 mmol/mg of protein), the enzyme glutathione S-transferase (190–5,827 μmol/min/mg of protein), and sulfhydryl groups (9.6–274.3 μmol cysteine/mg of protein). The ratio of reduced and oxidized glutathione indicated marked oxidative stress. In addition, dramatic changes in urease activity, which is connected with resistance of bacteria, were determined. Further, the effects of cadmium(II) ions on the metabolic pathways of arginine, β-glucosidase, phosphatase, N-acetyl β-d-glucosamine, sucrose, trehalose, mannitol, maltose, lactose, fructose and total proteins were demonstrated. A metabolomic profile of Staphylococcus aureus under cadmium(II) ion treatment conditions was completed seeking data about the possibility of cadmium(II) ion accumulation in cells. The results demonstrate potential in the application of microorganisms as modern biosensor systems based on biological components

Bio-Sensing of Cadmium(II) Ions Using Staphylococcus aureus†

Cadmium, as a hazardous pollutant commonly present in the living environment, represents an important risk to human health due to its undesirable effects (oxidative stress, changes in activities of many enzymes, interactions with biomolecules including DNA and RNA) and consequent potential risk, making its detection very important. New and unique technological and biotechnological approaches for solving this problems are intensely sought. In this study, we used the commonly occurring potential pathogenic microorganism Staphylococcus aureus for the determination of markers which could be used for sensing of cadmium(II) ions. We were focused on monitoring the effects of different cadmium(II) ion concentrations (0, 1.25, 2.5, 5, 10, 15, 25 and 50 μg mL−1) on the growth and energetic metabolism of Staphylococcus aureus. Highly significant changes have been detected in the metabolism of thiol compounds—specifically the protein metallothionein (0.79–26.82 mmol/mg of protein), the enzyme glutathione S-transferase (190–5,827 μmol/min/mg of protein), and sulfhydryl groups (9.6–274.3 μmol cysteine/mg of protein). The ratio of reduced and oxidized glutathione indicated marked oxidative stress. In addition, dramatic changes in urease activity, which is connected with resistance of bacteria, were determined. Further, the effects of cadmium(II) ions on the metabolic pathways of arginine, β-glucosidase, phosphatase, N-acetyl β-d-glucosamine, sucrose, trehalose, mannitol, maltose, lactose, fructose and total proteins were demonstrated. A metabolomic profile of Staphylococcus aureus under cadmium(II) ion treatment conditions was completed seeking data about the possibility of cadmium(II) ion accumulation in cells. The results demonstrate potential in the application of microorganisms as modern biosensor systems based on biological components

Salt tolerance of halophytes, research questions reviewed in the perspective of saline agriculture

Halophytes of the lower coastal salt marsh show increased salt tolerance, and under high salinity they grow faster than upper marsh species. We could not show reduced growth rate of halophytes compared with glycophytes when grown under non-saline conditions. This indicates limited energy costs associated with high-salt tolerance in plants of genera such as Salicornia, providing a good perspective of saline agriculture cultivating Salicornia as a vegetable crop.We show that halophytes do not occur on non-saline or inland sites because of a reduced growth rate at low soil salinity, but probably due to other ecological traits of glycophytic upper marsh species. These traits provide competitive advantage over lower salt marsh halophytes, such as earlier germination and increased growing season length.Some halophytic Amaranthaceae (Salicornioideae, Chenopodioideae and Suaedoideae) are not just highly salt tolerant, their growth rate is stimulated at a salinity range of 150–300 mM NaCl. Alternatively this may be described as depressed growth at low salinity.Selective pressure for such high-salt tolerance and salt stimulated growth likely occurred with prevailing arid climate and saline soil conditions. Under such conditions highly-salt tolerant succulent Salicornioideae, Chenopodioidea and Suaedoideae may have evolved about 65 Mya. In the context of evolution and diversication of land plants this origin of highly-salt tolerant succulent plants is relatively recent.Such high-salt tolerance might be characterized as constitutive in comparison with inducible (lower) salt tolerance of other dicotyledonae and monocotyledonae (Poaceae) species. Levels of salt tolerance of the latter type span a large range of low, intermediate to high-salt tolerance, but do not include salt stimulated growth. Salt tolerant traits of the latter inducible type appear to have evolved repeatedly and independently.Early highly-salt tolerant succulent Salicornioideae, Chenopodioidea and Suaedoideae were perennial and frost sensitive and occurred in warm temperate and Mediterranean regions. A shift from the perennial Sarcocornia to an annual life form has been phylogenetically dated circa 9.4–4.2 Mya and enabled evolution of annual hygrohalophytes in more northern coastal locations up to boreal and subarctic coastal sites avoiding damage of winter frost. Diversification of such hygrohalophytes was facilitated by polyploidization (e.g. occurrence of tetraploid and diploid Salicornia species), and a high degree of inbreeding allowing sympatric occurrence of Salicornia species in coastal salt marshes.High-level salt tolerance is probably a very complex polygenic trait. It is unlikely that glycophytes would accommodate the appropriate allelic variants at all the loci involved in halophyte salt tolerance. This might explain why attempts to improve crop salt tolerance through conventional breeding and selection have been unsuccessful to date.Genetic engineering provides a viable alternative, but the choice for the appropriate transgenes is hampered by a fundamental lack of knowledge of the mechanisms of salt tolerance in halophytes. The chances to identify the determinant genes through QTL analyses, or comparisons among near isogenic lines (NILS) are limited. Salt-tolerance is usually a species-wide trait in halophytes, and intra-specific divergence in salt tolerance in facultative halophytes seems to be often associated with chromosomal incompatibility.A variety of candidate salt tolerance genes been identified in Arabidopsis thaliana, among which genes encoding Na+ and K+ transporters, and genes involved in the general stress or anti-oxidant response, or in compatible solute metabolism. Many of these genes have been over-expressed in different glycophytic hosts, which usually appeared to alleviate, to some degree, the response to high salinity levels. However, with few exceptions, there are no indications that the same genes would be responsible for the superior salt tolerance in (eu)halophytes. Comparisons of gene expression and gene promoter activity patterns between halophytes and glycophytes are, with few exceptions, virtually lacking, which is a major omission in current day salt tolerance research.Full-genome transcriptomic comparisons between halophytes and related glycophytes through deep sequencing seem to be the most promising strategy to identify candidate genetic determinants of the difference in salt tolerance between halophytes and glycophytes.The most reliable validation of any candidate gene is through silencing the gene in the halophytic genetic background, preferably down to the level at which it is expressed in the glycophyte reference species. This requires genetically accessible halophyte models, which are not available to date, with the exception of Thellungiella halophila. However, more models are required, particularly because T. halophila is not a typical halophyte. Eventually, the pyramiding of validated salt tolerance genes under suitable promoters may be expected to be a viable strategy for crop salt tolerance improvement

Deep Brain Stimulation of Nucleus Accumbens Region in Alcoholism Affects Reward Processing

The influence of bilateral deep brain stimulation (DBS) of the nucleus nucleus (NAcc) on the processing of reward in a gambling paradigm was investigated using H2[15O]-PET (positron emission tomography) in a 38-year-old man treated for severe alcohol addiction. Behavioral data analysis revealed a less risky, more careful choice behavior under active DBS compared to DBS switched off. PET showed win- and loss-related activations in the paracingulate cortex, temporal poles, precuneus and hippocampus under active DBS, brain areas that have been implicated in action monitoring and behavioral control. Except for the temporal pole these activations were not seen when DBS was deactivated. These findings suggest that DBS of the NAcc may act partially by improving behavioral control