134 research outputs found
Exploring the performance of the spectrometer prisma in heavy zirconium and xenon mass regions
We present results from two recent runs which illustrate the performance of the PRISMA spectrometer in the proximity of the upper limit of its operational interval, namely 96Zr + 124Sn at Elab = 500 MeV and 136Xe + 208Pb at Elab = 930 MeV. In the latter run, the γ array CLARA also allowed us to identify previously unknown γ transitions in the nuclides 136Cs and 134I
First in-beam γ -ray study of the level structure of neutron-rich S 39
International audienceThe neutron-rich S39 nucleus has been studied using binary grazing reactions produced by the interaction of a 215-MeV beam of S36 ions with a thin Pb208 target. The magnetic spectrometer, PRISMA, and the γ-ray array, CLARA, were used in the measurements. Gamma-ray transitions of the following energies were observed: 339, 398, 466, 705, 1517, 1656, and 1724 keV. Five of the observed transitions have been tentatively assigned to the decay of excited states with spins up to (11/2−). The results of a state-of-the-art shell-model calculation of the level scheme of S39 using the SDPF-U effective interaction are also presented. The systematic behavior of the excitation energy of the first 11/2− states in the odd-A isotopes of sulfur and argon is discussed in relation to the excitation energy of the first excited 2+ states of the adjacent even-A isotopes. The states of S39 that have the components in their wave functions corresponding to three neutrons in the 1f7/2 orbital outside the N=20 core have also been discussed within the context of the 0 ℏω shell-model calculations presented here
Intruder negative-parity states of neutron-rich Si33
Yrast states in the neutron-rich 1433Si19 nucleus have been studied using binary grazing reactions produced by the interaction of a 215-MeV beam of S36 ions with a thin Pb208 target. An experimental setup that combines the large-acceptance magnetic spectrometer PRISMA and the high-efficiency γ-ray detection array CLARA was used in the experiment. Four new γ-ray photopeaks at energies of 971, 1724, 1772, and 2655 keV were observed and assigned to the Si33 level scheme. The experimental level scheme is compared with the results of 1ω p-sd-pf large-scale shell-model calculations using the recently developed PSDPFB effective interaction; good agreement is obtained. The structure of the populated states of Si33 is discussed within the context of an odd neutron coupled to states of the Si32 core. © 2010 The American Physical Society.This work was supported in part by the EPSRC (UK) and by the European Union under Contract No. RII3-CT-2004-506065. Five of us (D.O., M.B., A.H., K.K., and A.P.)acknowledge financial support from the EPSRC. Z.M.W acknowledges support from ORSAS and from the University of the West of Scotland. A.J. acknowledges financial supportfrom the Spanish Ministerio de Ciencia e Innovación under Contract Nos. FPA2007-66069 and FPA2009-13377-C02-02. Zs.D. acknowledges the financial support from OTKA Project No. K68801.Peer Reviewe
Gamma-ray spectroscopy of Cl using grazing reactions
Excited states of Cl were populated in grazing reactions
during the interaction of a beam of S ions of energy 215 MeV
with a Pb target. The combination of the PRISMA magnetic
spectrometer and the CLARA -ray detector array was used to identify the
reaction fragments and to detect their decay via -ray emission. A level
scheme for Cl is presented with tentative spin and parity assignments.
The level scheme is discussed within the context of the systematics of
neighboring nuclei and is compared with the results of state-of-the-art shell
model calculations.Comment: 8 pages, 6 figures and 2 tables Changes: Table II and Figure 5 have
been update
Highly Accurate Diagnosis of Pleural Tuberculosis by Immunological Analysis of the Pleural Effusion
Pleural TB is notoriously difficult to diagnose due to its paucibacillary nature yet it is the most common cause of pleural effusions in TB endemic countries such as The Gambia. We identified both cellular and soluble biomarkers in the pleural fluid that allowed highly accurate diagnosis of pleural TB compared to peripheral blood markers. Multi-plex cytokine analysis on unstimulated pleural fluid showed that IP-10 resulted in a positive likelihood ratio (LR) of 9.6 versus 2.8 for IFN-γ; a combination of IP-10, IL-6 and IL-10 resulted in an AUC of 0.96 and positive LR of 10. A striking finding was the significantly higher proportion of PPD-specific IFN-γ+TNF-α+ cell population (PPD-IGTA) in the pleural fluid compared to peripheral blood of TB subjects. Presence of this pleural PPD-IGTA population resulted in 95% correct classification of pleural TB disease with a sensitivity of 95% and specificity of 100%. These data suggest that analysis of the site of infection provides superior diagnostic accuracy compared to peripheral blood for pleural TB, likely due to the sequestration of effector cells at this acute stage of disease
Evaluation of immune responses in HIV infected patients with pleural tuberculosis by the QuantiFERON® TB-Gold interferon-gamma assay
<p>Abstract</p> <p>Background</p> <p>Diagnosis of tuberculous (TB) pleuritis is difficult and better diagnostic tools are needed. New blood based interferon-gamma (IFN-γ) tests are promising, but sensitivity could be low in HIV positive patients. The IFN-γ tests have not yet been validated for use in pleural fluid, a compartment with higher level of immune activation than in blood.</p> <p>Methods</p> <p>The QuantiFERON TB<sup>®</sup>-Gold (QFT-TB) test was analysed in blood and pleural fluid from 34 patients presenting with clinically suspected pleural TB. Clinical data, HIV status and CD4 cell counts were recorded. Adenosine deaminase activity (ADA) analysis and TB culture were performed on pleural fluid.</p> <p>Results</p> <p>The patients were categorised as 'confirmed TB' (n = 12), 'probable TB' (n = 16) and 'non-TB' pleuritis (n = 6) based on TB culture results and clinical and biochemical criteria. The majority of the TB patients were HIV infected (82%). The QFT-TB in pleural fluid was positive in 27% and 56% of the 'confirmed TB' and 'probable TB' cases, respectively, whereas the corresponding sensitivities in blood were 58% and 83%. Indeterminate results in blood (25%) were caused by low phytohemagglutinin (PHA = positive control) IFN-γ responses, significantly lower in the TB patients as compared to the 'non-TB' cases (p = 0.02). Blood PHA responses correlated with CD4 cell count (r = 0.600, p = 0.028). In contrast, in pleural fluid indeterminate results (52%) were caused by high Nil (negative control) IFN-γ responses in both TB groups. Still, the Nil IFN-γ responses were lower than the TB antigen responses (p < 0.01), offering a conclusive test for half of the patients. We did not find any correlation between blood CD4 cell count and IFN-γ responses in pleural fluid.</p> <p>Conclusion</p> <p>The QFT-TB test in blood could contribute to the diagnosis of TB pleuritis in the HIV positive population. Still, the number of inconclusive results is too high to recommend the commercial QFT-TB test for routine use in pleural fluid in a TB/HIV endemic resource-limited setting.</p
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What supervisors and universities can do to enhance doctoral student experience (and how they can help themselves)
Over the past two decades, there has been a flurry of government papers and policy reports worldwide calling for increased number and diversity of doctoral researchers and a broadening of the curriculum to meet the developing needs of respective national 'knowledge-driven' economies. This has been followed by position papers and best practice examples of employability skills development in boundary-crossing doctoral programmes, especially in response to these initiatives. However, there is a disassociation between this ample literature expounding the new doctorate with its broader remit, inclusivity and production of 'industry-ready' graduates, and the comparatively sparse literature on the doctoral candidates' experiences of their programmes and career readiness. Within this review, we briefly outline international government initiatives and examples of the responses by Life Science and Biomedical doctoral programmes to address these various challenges. Further, we explore the recent literature on the lived experience of doctoral researchers by examining their perception of the recent changes to the research context to make recommendations for universities and supervisors on how to better support an ever more diverse doctoral population for a wide range of career opportunities. Examples of how doctoral researchers themselves can make the best of currently available opportunities are also provided
Temperature Dependence of Backbone Dynamics in Human Ileal Bile Acid-Binding Protein: Implications for the Mechanism of Ligand Binding
Human ileal bile acid-binding protein (I-BABP), a member of the family of intracellular lipid binding proteins plays a key role in the cellular trafficking and metabolic regulation of bile salts. The protein has two internal and, according to a recent study, an additional superficial binding site and binds di- and trihydroxy bile salts with positive cooperativity and a high degree of site-selectivity. Previously, in the apo form, we have identified an extensive network of conformational fluctuations on the millisecond time scale, which cease upon ligation. Additionally, ligand binding at room temperature was found to be accompanied by a slight rigidification of picosecond-nanosecond (ps-ns) backbone flexibility. In the current study, temperature-dependent N-15 NMR spin relaxation measurements were used to gain more insight into the role of dynamics in human I-BABP-bile salt recognition. According to our analysis, residues sensing a conformational exchange in the apo state can be grouped into two clusters with slightly different exchange rates. The entropy-enthalpy compensation observed for both clusters suggests a disorder-order transition between a ground and a sparsely populated higher energy state in the absence of ligands. Analysis of the faster, ps-ns motion of N-15-H-1 bond vectors indicates an unusual nonlinear temperature-dependence for both ligation states. Intriguingly, while bile salt binding results in a more uniform response to temperature change throughout the protein, the temperature derivative of the generalized order parameter shows different responses to temperature increase for the two forms of the protein in the investigated temperature range. Analysis of both slow and fast motions in human I-BABP indicates largely different energy landscapes for the apo and halo states suggesting that optimization of binding interactions might be achieved by altering the dynamic behavior of specific segments in the protein
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