FFF Printing of Dual Material Molds

A new method for investment casting using additive manufacturing (AM) is proposed. The method can be performed using an FFF printing device having dual nozzles. One of the nozzle deposits a first material while the second nozzle prints a second material different from the first material. Both materials are used to create molds for casting so as to manufacture an end product. The proposed method allows for printing of complex mold geometries

High Temperature Nozzle Shield

This publication relates to a nozzle shield for shielding the nozzle of a print head. This nozzle shield has both a mechanical and a thermal functionality

Stiff brim

This publication relates to the field of additive manufacturing. It more specifically relates to a method of printing a stiff brim on a flexible build plate without influencing the quality of the 3D printed part

Age-related DNA methylation changes are tissue-specific with ELOVL2 promoter methylation as exception

Abstract Background The well-established association of chronological age with changes in DNA methylation is primarily founded on the analysis of large sets of blood samples, while conclusions regarding tissue-specificity are typically based on small number of samples, tissues and CpGs. Here, we systematically investigate the tissue-specific character of age-related DNA methylation changes at the level of the CpG, functional genomic region and nearest gene in a large dataset. Results We assembled a compendium of public data, encompassing genome-wide DNA methylation data (Illumina 450k array) on 8092 samples from 16 different tissues, including 7 tissues with moderate to high sample numbers (Dataset size range 96–1202, N total = 2858). In the 7 tissues (brain, buccal, liver, kidney, subcutaneous fat, monocytes and T-helper cells), we identified 7850 differentially methylated positions that gained (gain-aDMPs; cut-offs: P bonf ≤ 0.05, effect size ≥ 2%/10 years) and 4,287 that lost DNA methylation with age (loss-aDMPs), 92% of which had not previously been reported for whole blood. The majority of all aDMPs identified occurred in one tissue only (gain-aDMPs: 85.2%; loss-aDMPs: 97.4%), an effect independent of statistical power. This striking tissue-specificity extended to both the functional genomic regions (defined by chromatin state segmentation) and the nearest gene. However, aDMPs did accumulate in regions with the same functional annotation across tissues, namely polycomb-repressed CpG islands for gain-aDMPs and regions marked by active histone modifications for loss-aDMPs. Conclusion Our analysis shows that age-related DNA methylation changes are highly tissue-specific. These results may guide the development of improved tissue-specific markers of chronological and, perhaps, biological age

Epifania, recriação e ressentimento: fragmentos narrativos sobre a experiência da viagem na imigração italiana no Brasil

L'expérience du voyage dans le processus de l'immigration marque le premier contact avec l'inconnu. L'aventure de la traversée de l'océan signifie par conséquent l'abandon du seul monde tangible. Le nouveau monde va se dévoiler à l'émigrant au fur et à mesure que le navire avance en mer, en un mélange de représentations produites avant le départ et de nouvelles “idées-images” que l'expérience elle-même du voyage contribue à élaborer en continu. Au cours de ce processus, la lecture de "Sull'Oceano" d’Edmondo De Amicis permet une immersion dans ce monde fragmentaire d'images et des récits que l'émigrant va produire. Il tente par ce biais de comprendre sa propre expérience et son existence, en un monde entrecroisé de différentes expressions de la sensibilité. Lê nouveau monde se révèle, souvenir tout à la fois d’une terre que l’on a abandonnée et recréation d'une représentation pacificatrice

Epigenome-wide change and variation in DNA methylation in childhood:Trajectories from birth to late adolescence

DNA methylation (DNAm) is known to play a pivotal role in childhood health and development, but a comprehensive characterization of genome-wide DNAm trajectories across this age period is currently lacking. We have therefore performed a series of epigenome-wide association studies in 5019 blood samples collected at multiple time-points from birth to late adolescence from 2348 participants of two large independent cohorts. DNAm profiles of autosomal CpG sites (CpGs) were generated using the Illumina Infinium HumanMethylation450 BeadChip. Change over time was widespread, observed at over one-half (53%) of CpGs. In most cases, DNAm was decreasing (36% of CpGs). Inter-individual variation in linear trajectories was similarly widespread (27% of CpGs). Evidence for non-linear change and inter-individual variation in non-linear trajectories was somewhat less common (11 and 8% of CpGs, respectively). Very little inter-individual variation in change was explained by sex differences (0.4% of CpGs) even though sex-specific DNAm was observed at 5% of CpGs. DNAm trajectories were distributed non-randomly across the genome. For example, CpGs with decreasing DNAm were enriched in gene bodies and enhancers and were annotated to genes enriched in immune-developmental functions. In contrast, CpGs with increasing DNAm were enriched in promoter regions and annotated to genes enriched in neurodevelopmental functions. These findings depict a methylome undergoing widespread and often non-linear change throughout childhood. They support a developmental role for DNA methylation that extends beyond birth into late adolescence and has implications for understanding life-long health and disease. DNAm trajectories can be visualized at http://epidelta.mrcieu.ac.uk

Estimating soil aggregate size distribution from images using pattern spectra

A method for quantifying aggregate size distribution from the images of soil samples is introduced. Knowledge of soil aggregate size distribution can help to inform soil management practices for the sustainable growth of crops. While current in-field approaches are mostly subjective, obtaining quantifiable results in a laboratory is labour- and time-intensive. Our goal is to develop an imaging technique for quantitative analysis of soil aggregate size distribution, which could provide the basis of a tool for rapid assessment of soil structure. The prediction accuracy of pattern spectra descriptors based on hierarchical representations from attribute morphology are analysed, as well as the impact of using images of different quality and scales. The method is able to handle greater sample complexity than the previous approaches, while working with smaller samples sizes that are easier to handle. The results show promise for size analysis of soils with larger structures, and minimal sample preparation, as typical of soil assessment in agriculture

Hot Pockets

This publication relates a method of additive manufacturing 3D objects using thermoplastic materials. When depositing a layer of an 3D object, some traces within that layer are printed using an increased temperature to improve the strength of the object. The printing temperature may only be increased for those parts (i.e. pockets) that are enclosed by those parts that are printed using regular (i.e. lower) temperatures

MOESM13 of Age-related DNA methylation changes are tissue-specific with ELOVL2 promoter methylation as exception

Additional file 13: Table S5. Enriched GO terms for gain-aDMPs per tissue

MOESM8 of Age-related DNA methylation changes are tissue-specific with ELOVL2 promoter methylation as exception

Additional file 8: Figure S5. Chronological age against the Horvath’s predicted age for each of the 16 tissues