Development of a protective coating system for regeneratively cooled thrust chambers Final technical report, 1 Jul. 1967 - 31 Mar. 1969

Development of protective coating system for regeneratively cooled thrust chamber

Topoisomerase expression and amplification in solid tumours: Analysis of 24,262 patients.

BackgroundTopoisomerase I (TOPO1) and topoisomerase IIα (TOP2A) are specific targets of multiple chemotherapy drugs. Increased expression of TOPO1 protein and amplification of the TOP2A gene have been associated with treatment response in colorectal and breast cancers, respectively. TOPO1 and TOP2A may be potential therapeutic targets in other malignancies as well.Summary of methodsWe analysed TOPO1 protein expression and TOP2A gene amplification in patients (n = 24,262 specimens) with diverse cancers. Since HER2 and TOP2A co-amplification have been investigated for predictive value regarding anthracycline benefit, we analysed specimens for HER2 amplification as well.ResultsOverexpressed TOPO1 protein was present in 51% of the tumours. Four percent of the tumours had TOP2A amplification, with gallbladder tumours and gastroesophageal/oesophageal tumours having rates over 10%. Overall, 4903 specimens were assessed for both TOP2A and HER2 amplification; 129 (2.6%) had co-amplification. High rates (>40%) of HER2 amplification were seen in patients with TOP2A amplification in breast, ovarian, gastroesophageal/oesophageal and pancreatic cancer.ConclusionOur data indicate that increased TOPO1 expression and TOP2A amplification, as well as HER2 co-alterations, are present in multiple malignancies. The implications of these observations regarding sensitivity to chemotherapy not traditionally administered to these tumour types merits investigation

Preparation of refractory cermet structures for lithium compatibility testing

High-purity nitride and carbide cermets were synthesized for compatability testing in liquid lithium. A process was developed for the preparation of high-purity hafnium nitride powder, which was subsequently blended with tungsten powder or tantalum nitride and tungsten powders and fabricated into 3 in diameter billets by uniaxial hot pressing. Specimens were then cut from the billets for compatability testing. Similar processing techniques were applied to produce hafnium carbide and zirconium carbide cermets for use in the testing program. All billets produced were characterized with respect to chemistry, structure, density, and strength properties

Resumption Still Does Not Rescue Islands

Linguistic

NADH dehydrogenase of Trypanosoma brucei is important for efficient acetate production in bloodstream forms

This work was supported by a grant from the National Institutes of Health (USA) [AI 5R01 AI069057] to MP and AS; a grant from the Medical Research Council (UK) [G0600129] to AS; grants from the Centre National de la Recherche Scientifique (CNRS, France), The Université de Bordeaux, The Agence Nationale de la Recherche (ANR) [ACETOTRYP of the ANR-BLANC-2010 call and GLYCONOV of the “Générique” call] and the Laboratoire d’Excellence (LabEx) ParaFrap [grant ANR-11-LABX-0024] to FB; and a grant from the Wellcome Trust [093228] to TKS.In the slender bloodstream form, Trypanosoma brucei mitochondria are repressed for many functions. Multiple components of mitochondrial complex I, NADH:ubiquinone oxidoreductase, are expressed in this stage, but electron transfer through complex I is not essential. Here we investigate the role of the parasite’s second NADH:ubiquinone oxidoreductase, NDH2, which is composed of a single subunit that also localizes to the mitochondrion. While inducible knockdown of NDH2 had a modest growth effect in bloodstream forms, NDH2 null mutants, as well as inducible knockdowns in a complex I deficient background, showed a greater reduction in growth. Altering the NAD+/NADH balance would affect numerous processes directly and indirectly, including acetate production. Indeed, loss of NDH2 led to reduced levels of acetate, which is required for several essential pathways in bloodstream form T. brucei and which may have contributed to the observed growth defect. In conclusion our study shows that NDH2 is important, but not essential, in proliferating bloodstream forms of T. brucei, arguing that the mitochondrial NAD+/NADH balance is important in this stage, even though the mitochondrion itself is not actively engaged in the generation of ATP.Publisher PDFPeer reviewe

Dietary Restriction Induced Longevity is Mediated by Nuclear Receptor NHR-62 in Caenorhabditis elegans

Dietary restriction (DR) extends lifespan in a wide variety of species, yet the underlying mechanisms are not well understood. Here we show that the C. elegans HNF4a- related nuclear hormone receptor NHR-62 is required for metabolic and physiologic responses associated with DR-induced longevity. nhr-62 mediates the longevity of eat- 2 mutants, a genetic mimetic of dietary restriction, and blunts the longevity response of DR induced by bacterial food dilution at low nutrient levels. Metabolic changes associated with DR, including decreased Oil Red O staining, increased autophagy, and changes in fatty acid composition are partly reversed by mutation of nhr-62. Expression profiles reveal that several hundred genes induced by DR depend on the activity of NHR-62, including a putative lipase required for the DR response. This study provides critical evidence that nuclear hormone receptors regulate the DR response, suggesting hormonal and metabolic control of life span

Exile Vol. LVII

Spence, Caroline:To Lose a Brother 7Frances in Three Parts 8-11 Current, Abby: February\u27s Belly 12Selkie Woman 13-14 Callahan, Meghan: Whirl 15Halloween 25-26cool 27-29Faith 39-40Bookish (cover) Eden, Tristan: Runaway 16Sharkey\u27s Philadelphia 17 Heestand, Ashley: Tragedy, 1979 18-24 Persia, Danny: 20,000 Leagues Under the Sea 30-33Hymn to Satan 34-37 DiMartini, Amanda: untitled 38 Bullock, Karen: Gypsies 41-42 Swensson, Ellie: Phenomenology 43-44 Snow 45-47 Moran, Megan: untitled 48 untitled 58 Whites, Shawn: The Woman Across the Alley 49-57 Roozeboom, Nikki: Stain 59Distant 60-61 Ferguson, Brittani: That Dusty Italian Dugout 62-63 Gateway 64 Burdoff, Holly: Transitions 6

Two neutrino positron double beta decay of 106^{106}Cd for 0+→0+0^+ \to 0^+ transition

The two neutrino positron double beta decay of $^{106}$Cd for 0$^{+} \to$ 0$^{+}$ transition has been studied in the Hartree-Fock-Bogoliubov model in conjunction with the summation method. In the first step, the reliability of the intrinsic wave functions of $^{106}$Cd and $^{106}$Pd nuclei has been tested by comparing the theoretically calculated results for yrast spectra, reduced $B(E2$:$0^{+}\to 2^{+})$ transition probabilities, quadrupole moments $Q(2^{+})$ and gyromagnetic factors $g(2^{+})$ with the available experimental data. In the second step, the nuclear transition matrix element $M_{2\nu}$ and the half-life $T_{1/2}^{2\nu}$ for 0$^{+}\to$ 0$^{+}$ transition have been calculated with these wave functions. Moreover, we have studied the effect of deformation on nuclear transition matrix element M$_{2\nu}.$Comment: 20 page