Human identification through advanced forensic mass spectrometry of blood and fingermarks

Matrix assisted laser desorption ionisation (MALDI) mass spectrometry (MS) has been demonstrated in recent years to be an effective toolbox in the detection and identification of substances of forensic relevance. The goal of this PhD programme was to develop analytical protocols to obtain novel molecular information from two biological sample types; fingermarks and bloodstains. MALDI MS was employed to acquire mass spectrometry profiling (MSP) and mass spectrometry imaging (MSI) data from these two common evidence types to generate biological and chemical information towards a molecular ‗suspect profile‘. The use of MALDI MS to acquire data on the peptide and protein composition of fingermarks, in combination with advanced statistical processing was developed to investigate the determination of sex from fingermarks. A blind validation study was conducted for the robust, multifaceted identification of human and animal blood, other human biofluids and interferent substances from a large sample set of stains and fingermarks. MALDI MS was employed in combination with a ‗bottom-up‘ proteomic approach for the determination of biological matrices through the identification of proteotypic peptides. MALDI MS was also utilised for the determination of a subset of haemoglobin variants in human blood, through the detection of proteotypic peptides, employing a ‗bottom-up‘ proteomic approach. The detection of haemoglobin variants in blood encountered at a crime scene has implications towards associative evidence of the presence of an individual, which grows in significance the rarer (and less prevalent) the variant. The application of these three techniques towards these two biological matrices, and the validation data acquired for each, demonstrates their analytical capabilities. In all cases they have been demonstrated to be compatible with visualisation techniques (both fingermark enhancement techniques and blood enhancement techniques, respectively), showing potential for their integration into the current operational workflows for both fingermarks and blood. In conclusion, although only demonstrated under laboratory controlled conditions, MALDI MS shows promise towards the future analysis of these biological matrices within evidence recovery and investigative workflows, when specific molecular information may be sought

Why garden? – Attitudes and the perceived health benefits of home gardening

Domestic (home) gardens provide opportunities for psychological and physical health benefits, yet these environments have received less attention in terms of their therapeutic value compared to other urban green spaces. This is despite their ubiquity and the popularity of gardening as a pastime. This research explored why residents engaged with gardening and the extent to which they recognised any health benefits from the activity. A questionnaire was distributed electronically within the UK, with 5766 gardeners and 249 non-gardeners responding. Data were collated on factors including garden typology, frequency of gardening and individual perceptions of health and well-being. Significant associations were found between improvements in well-being, perceived stress and physical activity and more frequent gardening. Gardening on a frequent basis i.e. at least 2–3 times a week, corresponded with greatest perceived health benefits. Improving health, however, was not the prime motivator to garden, but rather the direct pleasure gardening brought to the participants. There was evidence that satisfaction with one's front garden and the time spent in it increased as the proportion of vegetation was enhanced. The data supports the notion that domestic gardens should be given greater prominence in urban planning debates, due to the role they play in providing health benefits

Pre-validation of a MALDI MS proteomics-based method for the reliable detection of blood and blood provenance

Abstract: The reliable identification of blood, as well as the determination of its origin (human or animal) is of great importance in a forensic investigation. Whilst presumptive tests are rapid and deployed in situ, their very nature requires confirmatory tests to be performed remotely. However, only serological tests can determine blood provenance. The present study improves on a previously devised Matrix Assisted Laser Desorption Ionisation Mass Spectrometry (MALDI MS)—proteomics based method for the reliable detection of blood by enabling the determination of blood provenance. The overall protocol was developed to be more specific than presumptive tests and faster/easier than the gold standard liquid chromatography (LC) MS/MS analysis. This is considered a pre-validation study that has investigated stains and fingermarks made in blood, other biofluids and substances that can elicit a false-positive response to colorimetric or presumptive tests, in a blind fashion. Stains and marks were either untreated or enhanced with a range of presumptive tests. Human and animal blood were correctly discriminated from other biofluids and non-biofluid related matrices; animal species determination was also possible within the system investigated. The procedure is compatible with the prior application of presumptive tests. The refined strategy resulting from iterative improvements through a trial and error study of 56 samples was applied to a final set of 13 blind samples. This final study yielded 12/13 correct identifications with the 13th sample being correctly identified as animal blood but with no species attribution. This body of work will contribute towards the validation of MALDI MS based methods and deployment in violent crimes involving bloodshed

Measurement of the rate of nu_e + d --> p + p + e^- interactions produced by 8B solar neutrinos at the Sudbury Neutrino Observatory

Solar neutrinos from the decay of $^8$B have been detected at the Sudbury Neutrino Observatory (SNO) via the charged current (CC) reaction on deuterium and by the elastic scattering (ES) of electrons. The CC reaction is sensitive exclusively to nu_e's, while the ES reaction also has a small sensitivity to nu_mu's and nu_tau's. The flux of nu_e's from ^8B decay measured by the CC reaction rate is \phi^CC(nu_e) = 1.75 +/- 0.07 (stat)+0.12/-0.11 (sys.) +/- 0.05(theor) x 10^6 /cm^2 s. Assuming no flavor transformation, the flux inferred from the ES reaction rate is \phi^ES(nu_x) = 2.39+/-0.34 (stat.)+0.16}/-0.14 (sys) x 10^6 /cm^2 s. Comparison of \phi^CC(nu_e) to the Super-Kamiokande Collaboration's precision value of \phi^ES(\nu_x) yields a 3.3 sigma difference, providing evidence that there is a non-electron flavor active neutrino component in the solar flux. The total flux of active ^8B neutrinos is thus determined to be 5.44 +/-0.99 x 10^6/cm^2 s, in close agreement with the predictions of solar models.Comment: 6 pages (LaTex), 3 figures, submitted to Phys. Rev. Letter

What Do We Know About Neuropsychological Aspects Of Schizophrenia?

Application of a neuropsychological perspective to the study of schizophrenia has established a number of important facts about this disorder. Some of the key findings from the existing literature are that, while neurocognitive impairment is present in most, if not all, persons with schizophrenia, there is both substantial interpatient heterogeneity and remarkable within-patient stability of cognitive function over the long-term course of the illness. Such findings have contributed to the firm establishment of neurobiologic models of schizophrenia, and thereby help to reduce the social stigma that was sometimes associated with purely psychogenic models popular during parts of the 20th century. Neuropsychological studies in recent decades have established the primacy of cognitive functions over psychopathologic symptoms as determinants of functional capacity and independence in everyday functioning. Although the cognitive benefits of both conventional and even second generation antipsychotic medications appear marginal at best, recognition of the primacy of cognitive deficits as determinants of functional disability in schizophrenia has catalyzed recent efforts to develop targeted treatments for the cognitive deficits of this disorder. Despite these accomplishments, however, some issues remain to be resolved. Efforts to firmly establish the specific neurocognitive/neuropathologic systems responsible for schizophrenia remain elusive, as do efforts to definitively demonstrate the specific cognitive deficits underlying specific forms of functional impairment. Further progress may be fostered by recent initiatives to integrate neuropsychological studies with experimental neuroscience, perhaps leading to measures of deficits in cognitive processes more clearly associated with specific, identifiable brain systems

A systematic review of how emotional self-awareness is defined and measured when comparing autistic and non-autistic groups

We would like to sincerely thank all the authors who shared their data with us. We would also like to thank Ira Lesser, Taylor Graeme, and Arvid Heiberg for kindly sharing their articles for the historical review. Review was conduced as part of CFH's PhD studies. We would like to thank the Northwood Trust, UK for their financial support for this research. Research data available upon request from first author.Peer reviewedPublisher PD