Bacillus endophyticus: Symbiotic Bacterium in Subterranean Termites Intestine (Blattodea: Termitoidae) from Bogor, Indonesia

Rayap merupakan serangga sosial yang berperan penting dalam perputaran siklus nutrisi. Di dalam sistem pencernaan rayap, terdapat simbion yang membantu proses degradasi selulosa. Penelitian ini bertujuan mengisolasi bakteri simbion yang terdapat di dalam saluran cerna rayap tanah. Penelitian diawali dengan koleksi rayap tanah di Kampus IPB University, diikuti isolasi bakteri simbion dari saluran cerna belakang (proktodeum) yang kemudian diidentifikasi berdasarkan morfologi, fisiologi, dan molekuler menggunakan gen 16S rRNA. Enam rayap tanah yang diperoleh adalah Macrotermes gilvus, Odontotermes javanicus, Microtermes insperatus, dan Capritermes mohri (Famili Termitidae); Schedorhinotermes javanicus dan Coptotermes curvignathus (Famili Rhinotermitidae). Dari enam rayap diperoleh 43 isolat dan satu isolat yang memiliki karakter umum. Isolat bakteri kode 8A_27F berwarna putih, elevasi seperti tombol dengan tepian licin. Dari uji fisiologis, isolat ini termasuk ke dalam bakteri Gram positif, berspora, dan bersifat aerob. Identifikasi dengan gen 16S rRNA menunjukkan bahwa isolat bakteri tersebut memiliki tingkat kemiripan sebesar 98% dengan spesies Bacillus endophyticus.   Kata kunci: bakteri, rayap, 16S rRNA, simbion, proktodeu

PATOGENISITAS BEBERAPA ISOLAT CENDAWAN ENTOMOPATOGEN TERHADAP COPTOTERMES CURVIGNATHUS HOLMGREN DAN SCHEDORHINOTERMES JAVANICUS KEMMER.

Pengendalian hayati rayap C. curvignathus dan S. javanicus dengan menggunakan beberapa isolat cendawan entomopatogen merupakan salah satu alternatif untuk pengendalian rayap tanah yang ramah lingkungan. Penelitian ini bertujuan untuk mempelajari patogenisitas cendawan entomopatogen isolat Indonesia Metarhizium anisopliae, M. brunneum, B. bassiana, dan Myrothecium roridum dan menguji keefektifannya terhadap rayap tanah C. curvignathus dan S. Javanicus. Uji patogenisitas beberapa isolat cendawan entomopatogen terhadap C. curvignathus dan S. javanicus dilakukan dengan pencelupan rayap ke dalam suspensi konidia, masing-masing dengan kerapatan 0,105,5x105,106,5x106,dan 107 konidia/ml, setiap perlakuan diulang lima kali. Sporulasi pada tubuh rayap dihitung dari rayap C. curvignathus yang telah mati pada uji patogenisitas. Data yang diperoleh dianalisis dengan sidik ragam dan uji lanjut selang ganda Duncan pada taraf nyata 5%. Hubungan kerapatan konidia dengan mortalitas dan waktu aplikasi dengan mortalitas diolah dengan analisis probit. Berdasarkan hasil analisis probit dapat diperoleh nilai LC dan LT. Patogenisitas M. brunneum terhadap rayap S. javanicus dan C. curvignathus lebih tinggi dibandingkan dengan M. anisopliae, Beauveria bassiana, dan Myrothecium roridum. Sporulasi M. brunneum lebih tinggi dari pada M. roridum maupun B. bassiana dan sporulasi terendah terdapat pada B. bassiana. Viabilitas M. brunneum tidak berbeda nyata dengan M. roridum dan viabilitas B. bassiana berbeda nyata dengan M. roridum. Kata-kata kunci: Cendawan entomopatogen, M. brunneum, rayap

POTENTIAL OF CLOVE ESSENTIAL OIL AGAINST SUBTERRANEAN TERMITE Macrotermes gilvus HAGEN (BLATTODEA: TERMITIDAE)

Macrotermes gilvus (subterranean termite) can play dual roles as decomposers of natural wood and pest in plantations and urban area. The damages caused by termites in the urban area reaches 35% and in the wood industry can reach 40%. Nowadays, termite control is still carried out by the application of synthetic termiticide. In termites’ case, environmental pollution issue is more important than the resistance of termite to termiticide, so it’s necessary to find alternative termiticide from natural materials. This study examined the potential of clove essential oils to control M. gilvus with two methods. The test methods were oil residue on filter paper and soil in a glass tube H. Clove essential oil on filter paper was applied and dried for one minute, after that put in the petri dish. For soil treatment, the soil was soaked with clove essential oil for one hour and dried for one day, after that the soil treatment put into the bridge tube. Each method was infested by 50 termites with ratio 90% workers and 10% soldiers. Termite mortality on treated filter paper was observed after 72 hours, and on treated soil were observed daily until 7 days. The result showed that clove essential oils caused mortality of M. gilvus more than 90% after consumed and contacted on treated filter paper and contacted on treated soil with concentration 0.80% and 1.20%, respectively. Based on the results, clove essential oil has the potential to be developed and can be used as an alternative against termites, that was environmentally friendly

Preferensi Kecoa Amerika Periplaneta americana (L.) (Blattaria: Blattidae) terhadap Berbagai Kombinasi Umpan

Currently, most cockroach control in urban environment relies on bait application. Aversion of some commercial bait by cockroach cause failure to control cockroach problems. Developing local bait can improve this situation. This research was aimed to find proper combination of some cockroach diet to be formulated in effective bait. Research was conducted in Department of Plant Protection, Bogor Agricultural University, since November 2007-March 2008. Combination of peanut butter, chicken egg, and strawberry jam seem to be a candidate of effective bait for local cockroach. KEY WORDS: American cockroach, bait, combination, preferenc

Aplikasi Fosfin Formulasi Cair Terhadap Thrips Parvispinus Karny (Thysanoptera: Thripidae) Pada Bunga Potong Krisan

Chrysanthemum (Chrysanthemum indicum) as one of horticultural commodities has a good prospect to be developed as source of country revenue. The export of cut flowers of chrysanthemum from Indonesia tends to increase year by year. However, Thrips parvispinus Karny (Thysanoptera: Thripidae) which is major insect pest of chrysanthemum often attacks this flower in the field until postharvest causing the decrease of quality of flowers. The poor quality of cut flowers of chrysanthemum including the presence of T. parvispinus will be rejected by foreign businesses. Therefore, control measures of this insect pest by quarantine officers are important. Treatment can be done using liquified formulation phosphine fumigant. The objectives of this study were to determine the concentration of liquified formulation phosphine and exposure time which will were effectively kill T. parvispinus and to evaluate the quality of cut flowers of chrysanthemums treated with phosphine fumigant. T. parvispinus was exposed to liquied formulation phosphine at 9 concentrations (0, 25, 50, 75, 100, 125, 150, 175 and 200 ppm) with 3 exposure times (1, 3, and 6 hours). Moreover, validation test of concentration nd exposure time of liquified formulation phosphine on cut flowers of chrysanthemum was conducted to evaluate the quality of chrysanthemum cut flowers of treated with phosphine fumigant. Results showed that liquified formulation of phosphine at concentration of 200 ppm and on 1 hour of exposure time completely killed T. parvispinus by 100% mortality. At 200 ppm and exposure time until 6 hours did not affect the quality of cut flowers of chrysanthemum

Perilaku Kunjungan dan Efisiensi Penyerbukan Heterotrigona itama (Cockerell) dan Tetragonula laeviceps (Smith) (Hymenoptera: Apidae) pada Labu Siam

Main insect pollinator of chayote in the neotropics is the stingless bees. In Indonesia, there is no information available on chayote pollination. Research was conducted with the objective to study the flowering phenology of chayote and the role of two specieses of stingless bees Heterotrigona itama (Cockerell) and Tetragonula laeviceps (Smith) (Hymenoptera: Apidae) on chayote pollination. Measurements included the number of staminate and pistillate flowers, volume of nectar and sugar concentration, floral handling time and visitation rate, and fruit set. Chayote plants produced more staminate flowers than pistillate flowers, with the ratio of 18:1. There was no difference in nectar volume and sugar concentration between staminate and pistillate flowers. H. itama and T. laeviceps visited both type of flowers, with the peak of daily visitation were occurred at 08:30-10:30 am local times. There were significant differences in the floral handling time and foraging rate of H. itama and T. laeviceps. Pollen load was significantly higher in H. itama (2137.50±184.49 grains) than those in T. laeviceps (1675.00±110.47 grains). H. itama seemed to deposite more pollen (14.1±4.1 grains) on stigma than T. laeviceps (9.8±3.1 grains). Flowers prevented from insect visits resulted in zero fruit set. Single flower visit by H. itama and T. laeviceps resulted in 60 and 40% fruit set, respectively. Higher percent of fruit set (80-85%) was noticed in open-pollination, suggesting that multiple visits by insect pollinators might increase the chances of successful pollination

Fumigant and Repellent Effects of Essential Oil Fractions of Mentha piperita against Tribolium castaneum (Coleoptera: Tenebrionidae)

Tribolium castaneum is one of the important stored-product pests in tropical countries. Fumigation with phosphine is the most commonly method to control T. castaneum. However, long-term application of phosphine will cause resistance. The aims of this research were to study the effects of fumigant and repellent of peppermint oil Mentha piperita fraction against T. castaneum, and to identify the compounds contained in the active fraction. This research was conducted in Entomology Laboratory Seameo Biotrop and Forensic Laboratory of Indonesian Police Headquarters Jakarta, from January to October 2016. Peppermint oil was extracted by distillation from the leaves of M. piperita. Peppermint oil was fractionated by counter-current distribution method using three solvents: n-hexane, ethyl acetate, and methanol. Then, the three fractions were tested as fumigant and repellent against T. castaneum. The active fraction of essential oils were identified by GC-MS. The n-hexane fraction of peppermint (HFP) was the active fraction caused mortality on larvae and adults of  T. castaneum at 72 hours after fumigation. The LC95 values for the adults and  larvae was 1.9 % and 9.43 % , respectively. Sublethal concentration of HFP showed inhibitory activity on larvae development. Percentage of pupae transformed from treated larvae was 48.67-74.91 % and percentage of adults emerged was 9.12-16.59 %. The repellent activity of HFP on adults of T. castaneum was 86.7 % at 72 hours after treatment. The dominant compounds of HFP was β-pinene, limonene, isopulegol, menthone, isomenthon, menthol, pulegone, and trans-carane. The HFP or its compounds was potential to be develoved as botanical insecticides.Tribolium castaneum is one of the important stored-product pests in tropical countries. Fumigation with phosphine is the most commonly method to control T. castaneum. However, long-term application of phosphine will cause resistance. The aims of this research were to study the effects of fumigant and repellent of peppermint oil Mentha piperita fraction against T. castaneum, and to identify the compounds contained in the active fraction. This research was conducted in Entomology Laboratory Seameo Biotrop and Forensic Laboratory of Indonesian Police Headquarters Jakarta, from January to October 2016. Peppermint oil was extracted by distillation from the leaves of M. piperita. Peppermint oil was fractionated by counter-current distribution method using three solvents: n-hexane, ethyl acetate, and methanol. Then, the three fractions were tested as fumigant and repellent against T. castaneum. The active fraction of essential oils were identified by GC-MS. The n-hexane fraction of peppermint (HFP) was the active fraction caused mortality on larvae and adults of  T. castaneum at 72 hours after fumigation. The LC95 values for the adults and  larvae was 1.9 % and 9.43 % , respectively. Sublethal concentration of HFP showed inhibitory activity on larvae development. Percentage of pupae transformed from treated larvae was 48.67-74.91 % and percentage of adults emerged was 9.12-16.59 %. The repellent activity of HFP on adults of T. castaneum was 86.7 % at 72 hours after treatment. The dominant compounds of HFP was β-pinene, limonene, isopulegol, menthone, isomenthon, menthol, pulegone, and trans-carane. The HFP or its compounds was potential to be develoved as botanical insecticides