Aeromechanical stability of a hingeless rotor in hover and forward flight: Analysis and wind tunnel tests

A research effort of analysis and testing was conducted to investigate the ground resonance phenomenon of a soft in-plane hingeless rotor. Experimental data were obtained using a 9 ft. (2.74 m) diameter model rotor in hover and forward flight. Eight model rotor configurations were investigated. Configuration parameters included pitch flap coupling, blade sweep and droop, and precone of the blade feathering axis. An analysis based on a comprehensive analytical model of rotorcraft aerodynamics and dynamics was used. The moving block was used to experimentally determine the regressing lead lag mode damping. Good agreement was obtained between the analysis and test. Both analysis and experiment indicated ground resonance instability in hover. An outline of the analysis, a description of the experimental model and procedures, and comparison of the analytical and experimental data are presented

Fuzzy model based multivariable predictive control design for rapid and efficient speed-sensorless maximum power extraction of renewable wind generators

Introduction. A wind energy conversion system needs a maximum power point tracking algorithm. In the literature, several works have interested in the search for a maximum power point wind energy conversion system. Generally, their goals are to optimize the mechanical rotation or the generator torque and the direct current or the duty cycle switchers. The power output of a wind energy conversion system depends on the accuracy of the maximum power tracking controller, as wind speed changes constantly throughout the day. Maximum power point tracking systems that do not require mechanical sensors to measure the wind speed offer several advantages over systems using mechanical sensors. The novelty. The proposed work introduces an intelligent maximum power point tracking technique based on a fuzzy model and multivariable predictive controller to extract the maximum energy for a small-scale wind energy conversion system coupled to the electrical network. The suggested algorithm does not need the measurement of the wind velocity or the knowledge of turbine parameters. Purpose. Building an intelligent maximum power point tracking algorithm that does not use mechanical sensors to measure the wind speed and extracts the maximum possible power from the wind generator, and is simple and easy to implement. Methods. In this control approach, a fuzzy system is mainly utilized to generate the reference DC-current corresponding to the maximum power point based on the changes in the DC-power and the rectified DC-voltage. In contrast, the fuzzy model-based multivariable predictive regulator follows the resultant reference current with minimum steady-state error. The significant issues of the suggested maximum power point tracking method, such as the detailed design process and implementation of the two controllers, have been thoroughly investigated and presented. The considered maximum power point tracking approach has been applied to a wind system driving a 5 kW permanent magnet synchronous generator in variable speed mode through the simulation tests. Practical value. A practical implementation has been executed on a 5 kW test bench consisting of a dSPACEds1104 controller board, permanent magnet synchronous generator, and DC-motor drives to confirm the simulation results. Comparative experimental results under varying wind speed have confirmed the achievable significant performance enhancements on the maximum wind energy generation and overall system response by using the suggested control method compared with a traditional proportional integral maximum power point tracking controller.Вступ. Система перетворення енергії вітру потребує алгоритму відстеження точки максимальної потужності. У літературі є кілька робіт, присвячених пошуку системи перетворення енергії вітру із точкою максимальної потужності. Як правило, їх метою є оптимізація механічного обертання або моменту, що крутить, генератора і перемикачів постійного струму або робочого циклу. Вихідна потужність системи перетворення енергії вітру залежить від точності контролера стеження за максимальною потужністю, оскільки швидкість вітру постійно змінюється протягом дня. Системи стеження за точками з максимальною потужністю, яким не потрібні механічні датчики для вимірювання швидкості вітру, мають ряд переваг у порівнянні з системами, що використовують механічні датчики. Новизна. Пропонована робота представляє інтелектуальний метод відстеження точки максимальної потужності, заснований на нечіткій моделі та багатопараметричному прогнозуючому контролері, для отримання максимальної енергії для маломасштабної системи перетворення енергії вітру, підключеної до електричної мережі. Пропонований алгоритм не вимагає вимірювання швидкості вітру або знання параметрів турбіни. Мета. Побудова інтелектуального алгоритму відстеження точки максимальної потужності, який не використовує механічні датчики для вимірювання швидкості вітру та витягує максимально можливу потужність з вітрогенератора, а також простий та зручний у реалізації. Методи. У цьому підході до управління нечітка система в основному використовується для генерування еталонного постійного струму, що відповідає точці максимальної потужності, на основі змін потужності постійного струму та постійної випрямленої напруги. Навпаки, багатопараметричний прогнозуючий регулятор на основі нечіткої моделі слідує за результуючим еталонним струмом з мінімальною помилкою, що встановилася. Істотні проблеми запропонованого методу відстеження точки максимальної потужності, такі як процес детального проектування та реалізація двох контролерів, були ретельно досліджені та представлені. Розглянутий підхід до відстеження точки максимальної потужності був застосований до вітрової системи, що приводить у дію синхронний генератор з постійними магнітами потужністю 5 кВт у режимі змінної швидкості за допомогою моделювання. Практична цінність. Для підтвердження результатів моделювання було виконано практичну реалізацію на випробувальному стенді потужністю 5 кВт, що складається з плати контролера dSPACEds1104, синхронного генератора з постійними магнітами та електроприводів з двигунами постійного струму. Порівняльні експериментальні результати при різній швидкості вітру підтвердили значні поліпшення продуктивності з максимального вироблення енергії вітру і загального відгуку системи при використанні запропонованого методу управління в порівнянні з традиційним пропорційно-інтегральним контролером спостереження за точкою максимальної потужності

Disclosure of cholesterol recognition motifs in transmembrane domains of the human nicotinic acetylcholine receptor

Cholesterol influences ion-channel function, distribution and clustering in the membrane, endocytosis, and exocytic sorting of the nicotinic acetylcholine receptor (AChR). We report the occurrence of a cholesterol recognition motif, here coined “CARC”, in the transmembrane regions of AChR subunits that bear extensive contact with the surrounding lipid, and are thus optimally suited to convey cholesterol-mediated signaling from the latter. Three cholesterol molecules could be docked on the transmembrane segments of each AChR subunit, rendering a total of 15 cholesterol molecules per AChR molecule. The CARC motifs contribute each with an energy of interaction between 35 and 52 kJ.mol−1, adding up to a total of about 200 kJ.mol−1 per receptor molecule, i.e. ∼40% of the lipid solvation free energy/ AChR molecule. The CARC motif is remarkably conserved along the phylogenetic scale, from prokaryotes to human, suggesting that it could be responsible for some of the above structural/functional properties of the AChR

A Novel Inactivated Intranasal Respiratory Syncytial Virus Vaccine Promotes Viral Clearance without Th2 Associated Vaccine-Enhanced Disease

Respiratory syncytial virus (RSV) is a leading cause of bronchiolitis and pneumonia in young children worldwide, and no vaccine is currently available. Inactivated RSV vaccines tested in the 1960's led to vaccine-enhanced disease upon viral challenge, which has undermined RSV vaccine development. RSV infection is increasingly being recognized as an important pathogen in the elderly, as well as other individuals with compromised pulmonary immunity. A safe and effective inactivated RSV vaccine would be of tremendous therapeutic benefit to many of these populations.In these preclinical studies, a mouse model was utilized to assess the efficacy of a novel, nanoemulsion-adjuvanted, inactivated mucosal RSV vaccine. Our results demonstrate that NE-RSV immunization induced durable, RSV-specific humoral responses, both systemically and in the lungs. Vaccinated mice exhibited increased protection against subsequent live viral challenge, which was associated with an enhanced Th1/Th17 response. In these studies, NE-RSV vaccinated mice displayed no evidence of Th2 mediated immunopotentiation, as has been previously described for other inactivated RSV vaccines.These studies indicate that nanoemulsion-based inactivated RSV vaccination can augment viral-specific immunity, decrease mucus production and increase viral clearance, without evidence of Th2 immune mediated pathology

Adaptive Oblivious Transfer and Generalization

International audienceOblivious Transfer (OT) protocols were introduced in the seminal paper of Rabin, and allow a user to retrieve a given number of lines (usually one) in a database, without revealing which ones to the server. The server is ensured that only this given number of lines can be accessed per interaction, and so the others are protected; while the user is ensured that the server does not learn the numbers of the lines required. This primitive has a huge interest in practice, for example in secure multi-party computation, and directly echoes to Symmetrically Private Information Retrieval (SPIR). Recent Oblivious Transfer instantiations secure in the UC framework suf- fer from a drastic fallback. After the first query, there is no improvement on the global scheme complexity and so subsequent queries each have a global complexity of O(|DB|) meaning that there is no gain compared to running completely independent queries. In this paper, we propose a new protocol solving this issue, and allowing to have subsequent queries with a complexity of O(log(|DB|)), and prove the protocol security in the UC framework with adaptive corruptions and reliable erasures. As a second contribution, we show that the techniques we use for Obliv- ious Transfer can be generalized to a new framework we call Oblivi- ous Language-Based Envelope (OLBE). It is of practical interest since it seems more and more unrealistic to consider a database with uncontrolled access in access control scenarii. Our approach generalizes Oblivious Signature-Based Envelope, to handle more expressive credentials and requests from the user. Naturally, OLBE encompasses both OT and OSBE, but it also allows to achieve Oblivious Transfer with fine grain access over each line. For example, a user can access a line if and only if he possesses a certificate granting him access to such line. We show how to generically and efficiently instantiate such primitive, and prove them secure in the Universal Composability framework, with adaptive corruptions assuming reliable erasures. We provide the new UC ideal functionalities when needed, or we show that the existing ones fit in our new framework. The security of such designs allows to preserve both the secrecy of the database values and the user credentials. This symmetry allows to view our new approach as a generalization of the notion of Symmetrically PIR

Bacillus cereus Spores Release Alanine that Synergizes with Inosine to Promote Germination

spores germinate in the presence of a single germinant, inosine, yet with a significant lag period. spores. spores appear to have developed a unique quorum-sensing feedback mechanism to monitor spore density and to coordinate germination

Increased 30-Day Mortality in Very Old ICU Patients with COVID-19 Compared to Patients with Respiratory Failure without COVID-19

Purpose: The number of patients ≥ 80 years admitted into critical care is increasing. Coronavirus disease 2019 (COVID-19) added another challenge for clinical decisions for both admission and limitation of life-sustaining treatments (LLST). We aimed to compare the characteristics and mortality of very old critically ill patients with or without COVID-19 with a focus on LLST. Methods: Patients 80 years or older with acute respiratory failure were recruited from the VIP2 and COVIP studies. Baseline patient characteristics, interventions in intensive care unit (ICU) and outcomes (30-day survival) were recorded. COVID patients were matched to non-COVID patients based on the following factors: age (± 2 years), Sequential Organ Failure Assessment (SOFA) score (± 2 points), clinical frailty scale (± 1 point), gender and region on a 1:2 ratio. Specific ICU procedures and LLST were compared between the cohorts by means of cumulative incidence curves taking into account the competing risk of discharge and death. Results: 693 COVID patients were compared to 1393 non-COVID patients. COVID patients were younger, less frail, less severely ill with lower SOFA score, but were treated more often with invasive mechanical ventilation (MV) and had a lower 30-day survival. 404 COVID patients could be matched to 666 non-COVID patients. For COVID patients, withholding and withdrawing of LST were more frequent than for non-COVID and the 30-day survival was almost half compared to non-COVID patients. Conclusion: Very old COVID patients have a different trajectory than non-COVID patients. Whether this finding is due to a decision policy with more active treatment limitation or to an inherent higher risk of death due to COVID-19 is unclear.info:eu-repo/semantics/publishedVersio

Photo-affinity labelling and biochemical analyses identify the target of trypanocidal simplified natural product analogues

This work was supported by the Leverhulme Trust (Grant number RL2012-025). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Current drugs to treat African sleeping sickness are inadequate and new therapies are urgently required. As part of a medicinal chemistry programme based upon the simplification of acetogenin-type ether scaffolds, we previously reported the promising trypanocidal activity of compound 1 , a bis-tetrahydropyran 1,4-triazole (B-THP-T) inhibitor. This study aims to identify the protein target(s) of this class of compound in Trypanosoma brucei to understand its mode of action and aid further structural optimisation. We used compound 3 , a diazirine- and alkyne-containing bi-functional photo-affinity probe analogue of our lead B-THP-T, compound 1 , to identify potential targets of our lead compound in the procyclic form T. brucei. Bi-functional compound 3 was UV cross-linked to its target(s) in vivo and biotin affinity or Cy5.5 reporter tags were subsequently appended by Cu(II)-catalysed azide-alkyne cycloaddition. The biotinylated protein adducts were isolated with streptavidin affinity beads and subsequent LC-MSMS identified the FoF1-ATP synthase (mitochondrial complex V) as a potential target. This target identification was confirmed using various different approaches. We show that (i) compound 1 decreases cellular ATP levels (ii) by inhibiting oxidative phosphorylation (iii) at the FoF1-ATP synthase. Furthermore, the use of GFP-PTP-tagged subunits of the FoF1-ATP synthase, shows that our compounds bind specifically to both the α- and β-subunits of the ATP synthase. The FoF1-ATP synthase is a target of our simplified acetogenin-type analogues. This mitochondrial complex is essential in both procyclic and bloodstream forms of T. brucei and its identification as our target will enable further inhibitor optimisation towards future drug discovery. Furthermore, the photo-affinity labeling technique described here can be readily applied to other drugs of unknown targets to identify their modes of action and facilitate more broadly therapeutic drug design in any pathogen or disease model.Publisher PDFPeer reviewe

Cardiac fibrosis in aging mice

Dystrophic cardiac calcinosis (DCC), also called epicardial and myocardial fibrosis and mineralization, has been detected in mice of a number of laboratory inbred strains, most commonly C3H/HeJ and DBA/2J. In previous mouse breeding studies between these DCC susceptible and the DCC-resistant strain C57BL/6J, 4 genetic loci harboring genes involved in DCC inheritance were identified and subsequently termed Dyscalc loci 1 through 4. Here, we report susceptibility to cardiac fibrosis, a sub-phenotype of DCC, at 12 and 20 months of age and close to natural death in a survey of 28 inbred mouse strains. Eight strains showed cardiac fibrosis with highest frequency and severity in the moribund mice. Using genotype and phenotype information of the 28 investigated strains, we performed genome-wide association studies (GWAS) and identified the most significant associations on chromosome (Chr) 15 at 72 million base pairs (Mb) (P < 10(-13)) and Chr 4 at 122 Mb (P < 10(-11)) and 134 Mb (P < 10(-7)). At the Chr 15 locus, Col22a1 and Kcnk9 were identified. Both have been reported to be morphologically and functionally important in the heart muscle. The strongest Chr 4 associations were located approximately 6 Mb away from the Dyscalc 2 quantitative trait locus peak within the boundaries of the Extl1 gene and in close proximity to the Trim63 and Cap1 genes. In addition, a single-nucleotide polymorphism association was found on chromosome 11. This study provides evidence for more than the previously reported 4 genetic loci determining cardiac fibrosis and DCC. The study also highlights the power of GWAS in the mouse for dissecting complex genetic traits.The authors thank Jesse Hammer and Josiah Raddar for technical assistance. Research reported in this publication was supported by the Ellison Medical Foundation, Parker B. Francis Foundation, and the National Institutes of Health (R01AR055225 and K01AR064766). Mouse colonies were supported by the National Institutes of Health under Award Number AG025707 for the Jackson Aging Center. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. The Jackson Laboratory Shared Scientific Services were supported in part by a Basic Cancer Center Core Grant from the National Cancer Institute (CA34196).This is the author accepted manuscript. The final version is available from Springer via http://dx.doi.org/10.1007/s00335-016-9634-