Balance control in pirouettes – what role does spotting play?

Rotations around the vertical axis are among the most often-performed dance movements. Especially in ballet, pirouettes take a prominent place in the movement vocabulary. However, evidence on balance control and coordination during pirouettes is scarce. So far, no studies have addressed the influence of the fundamental spotting technique on balance in pirouettes. Therefore, the aim of this presentation is to summarise findings from two different studies on balance control and the coordination of spotting in pirouettes and continuous rotations. Study A tested 24 intermediate ballet dancers for postural stability after turning 14 consecutive rotations either actively or passively on a rotating chair. In both conditions, participants turned once while adopting the spotting technique and once without spotting. Before and after the rotations, Centre-of-Pressure (COP) displacement in quiet stance was measured on a force plate and perception of vertigo after-effect was measured by self-assessment (Keshavarz & Hecht, 2011). Conditions were compared with repeated-measures ANOVA. Study B was conducted with eight intermediate dancers who performed double pirouettes with and without the spotting technique. Whole-body movement was measured with a three-dimensional motion capture system and COP displacement with a force plate. The following balance measures were calculated: topple angle, instantaneous axis, and displacement of the foot marker. The following spotting measures were calculated: duration of head towards front, and head-trunk dissociation. Study A showed that balance after turning with the spotting technique was better than turning without spotting (p=.047). It thus seems that spotting helps balance control after rotations. In study B, we could show that spotting also helps balance control during rotations (topple angle with spotting is smaller (M=5.8°, SD=1.1°) than while turning without spotting (M=7.1°, SD=1.2°; p>.001). Besides discussing the results of our studies in more detail, we will present the advantages and disadvantages of different performance measure in pirouettes. Valid, dance-specific measures are crucial to allow for the comparison and progress of studies in the field of dance science and further, to advance the understanding of the role of spotting in whole body rotations. This research brings novel insights to the methods for quantifying the complex movements of dance, ultimately to improve dance training and technique. References: Keshavarz, B., & Hecht, H. (2011). Validating an efficient method to quantify motion sickness. Human factors, 53(4), 415–426

ABCB1 (MDR1) polymorphisms and ovarian cancer progression and survival: A comprehensive analysis from the Ovarian Cancer Association Consortium and The Cancer Genome Atlas

<b>Objective</b> <i>ABCB1</i> encodes the multi-drug efflux pump P-glycoprotein (P-gp) and has been implicated in multi-drug resistance. We comprehensively evaluated this gene and flanking regions for an association with clinical outcome in epithelial ovarian cancer (EOC).<p></p> <b>Methods</b> The best candidates from fine-mapping analysis of 21 <i>ABCB1</i> SNPs tagging C1236T (rs1128503), G2677T/A (rs2032582), and C3435T (rs1045642) were analysed in 4616 European invasive EOC patients from thirteen Ovarian Cancer Association Consortium (OCAC) studies and The Cancer Genome Atlas (TCGA). Additionally we analysed 1,562 imputed SNPs around ABCB1 in patients receiving cytoreductive surgery and either ‘standard’ first-line paclitaxel–carboplatin chemotherapy (n = 1158) or any first-line chemotherapy regimen (n = 2867). We also evaluated ABCB1 expression in primary tumours from 143 EOC patients.<p></p> <b>Result</b> Fine-mapping revealed that rs1128503, rs2032582, and rs1045642 were the best candidates in optimally debulked patients. However, we observed no significant association between any SNP and either progression-free survival or overall survival in analysis of data from 14 studies. There was a marginal association between rs1128503 and overall survival in patients with nil residual disease (HR 0.88, 95% CI 0.77–1.01; p = 0.07). In contrast, <i>ABCB1</i> expression in the primary tumour may confer worse prognosis in patients with sub-optimally debulked tumours.<p></p> <b>Conclusion</b> Our study represents the largest analysis of <i>ABCB1</i> SNPs and EOC progression and survival to date, but has not identified additional signals, or validated reported associations with progression-free survival for rs1128503, rs2032582, and rs1045642. However, we cannot rule out the possibility of a subtle effect of rs1128503, or other SNPs linked to it, on overall survival.<p></p&gt

COVID-19 symptoms at hospital admission vary with age and sex: results from the ISARIC prospective multinational observational study

Background: The ISARIC prospective multinational observational study is the largest cohort of hospitalized patients with COVID-19. We present relationships of age, sex, and nationality to presenting symptoms. Methods: International, prospective observational study of 60 109 hospitalized symptomatic patients with laboratory-confirmed COVID-19 recruited from 43 countries between 30 January and 3 August 2020. Logistic regression was performed to evaluate relationships of age and sex to published COVID-19 case definitions and the most commonly reported symptoms. Results: ‘Typical’ symptoms of fever (69%), cough (68%) and shortness of breath (66%) were the most commonly reported. 92% of patients experienced at least one of these. Prevalence of typical symptoms was greatest in 30- to 60-year-olds (respectively 80, 79, 69%; at least one 95%). They were reported less frequently in children (≤ 18 years: 69, 48, 23; 85%), older adults (≥ 70 years: 61, 62, 65; 90%), and women (66, 66, 64; 90%; vs. men 71, 70, 67; 93%, each P < 0.001). The most common atypical presentations under 60 years of age were nausea and vomiting and abdominal pain, and over 60 years was confusion. Regression models showed significant differences in symptoms with sex, age and country. Interpretation: This international collaboration has allowed us to report reliable symptom data from the largest cohort of patients admitted to hospital with COVID-19. Adults over 60 and children admitted to hospital with COVID-19 are less likely to present with typical symptoms. Nausea and vomiting are common atypical presentations under 30 years. Confusion is a frequent atypical presentation of COVID-19 in adults over 60 years. Women are less likely to experience typical symptoms than men

Retrospective evaluation of whole exome and genome mutation calls in 746 cancer samples

Funder: NCI U24CA211006Abstract: The Cancer Genome Atlas (TCGA) and International Cancer Genome Consortium (ICGC) curated consensus somatic mutation calls using whole exome sequencing (WES) and whole genome sequencing (WGS), respectively. Here, as part of the ICGC/TCGA Pan-Cancer Analysis of Whole Genomes (PCAWG) Consortium, which aggregated whole genome sequencing data from 2,658 cancers across 38 tumour types, we compare WES and WGS side-by-side from 746 TCGA samples, finding that ~80% of mutations overlap in covered exonic regions. We estimate that low variant allele fraction (VAF < 15%) and clonal heterogeneity contribute up to 68% of private WGS mutations and 71% of private WES mutations. We observe that ~30% of private WGS mutations trace to mutations identified by a single variant caller in WES consensus efforts. WGS captures both ~50% more variation in exonic regions and un-observed mutations in loci with variable GC-content. Together, our analysis highlights technological divergences between two reproducible somatic variant detection efforts

Measuring a “spot”: a methodological approach to developing and defining dance-specific measures

Turns of ballet are rooted in complex perceptual and motor skills. Dancers commonly perform pirouettes of one to three rotations, while the most challenging turns, fouettés, can be performed with countless rotations, with grand-scale ballets culminating in the performance of 32 consecutive fouetté turns. Central to such rotation is spotting a dance-specific gaze technique. As the body rotates, the head and gaze remain fixed to one spot. When the fixation can no longer be sustained, the head quickly rotates, overtaking the body to return to the same spot. Although crucial to every turn, there is a lack of scientific insight to why dancers spot or what characterizes a successful spot. In preparation for a study investigating spotting in pirouettes and fouettés of professional dancers in May of 2017, we have analyzed a pilot study with intermediate dancers to find dance-specific measures for spotting and postural stability during rotations about the vertical axis. Sixteen dancers’ whole-body motion was measured with a three-dimensional motion capture system (Vicon-T20) as they performed double pirouettes to the right and left sides, and then as many fouettés as possible (n=5 ±2) on the preferred side. The participants performed the pirouettes and fouettés in the following randomized visual conditions: a dim room with no reference points, with an illuminated dot at eye-level, with a grid of dots, and with four dots in the periphery. In addressing our main inquiry of the role of spotting, the larger question arises – how do we measure a “spot”? We will present several measures, which allow for the analysis of postural stability and spotting in the highly complex and dynamic whole body movement of pirouettes and fouettés. Furthermore, we will discuss the advantages and disadvantages of each measure that led us to determine our optimal measure. Valid, dance-specific measures are crucial to allow for the comparison and progress of studies in the field of dance science and further, to advance the understanding of the role of spotting in whole body rotations. This research brings novel insights to the methods for quantifying dance technique and ultimately, to improve dance training and technique

ABCC4/MRP4 contributes to the aggressiveness of Myc-associated epithelial ovarian cancer.

Epithelial ovarian cancer (EOC) is a complex disease comprising discrete histological and molecular subtypes, for which survival rates remain unacceptably low. Tailored approaches for this deadly heterogeneous disease are urgently needed. Efflux pumps belonging to the ATP-binding cassette (ABC) family of transporters are known for roles in both drug resistance and cancer biology and are also highly targetable. Here we have investigated the association of ABCC4/MRP4 expression to clinical outcome and its biological function in endometrioid and serous tumors, common histological subtypes of EOC. We found high expression of ABCC4/MRP4, previously shown to be directly regulated by c-Myc/N-Myc, was associated with poor prognosis in endometrioid EOC (P = .001) as well as in a subset of serous EOC with a high-MYCN profile (C5/proliferative; P = .019). Transient siRNA-mediated suppression of MRP4 in EOC cells led to reduced growth, migration and invasion, with the effects being most pronounced in endometrioid and C5-like serous cells compared to non-C5 serous EOC cells. Sustained knockdown of MRP4 also sensitized endometrioid cells to MRP4 substrate drugs. Furthermore, suppression of MRP4 decreased the growth of patient-derived EOC cells in vivo. Together, our findings provide the first evidence that MRP4 plays an important role in the biology of Myc-associated ovarian tumors and highlight this transporter as a potential therapeutic target for EOC

ABCC4

Epithelial ovarian cancer (EOC) is a complex disease comprising discrete histological and molecular subtypes, for which survival rates remain unacceptably low. Tailored approaches for this deadly heterogeneous disease are urgently needed. Efflux pumps belonging to the ATP-binding cassette (ABC) family of transporters are known for roles in both drug resistance and cancer biology and are also highly targetable. Here we have investigated the association of ABCC4/MRP4 expression to clinical outcome and its biological function in endometrioid and serous tumors, common histological subtypes of EOC. We found high expression of ABCC4/MRP4, previously shown to be directly regulated by c-Myc/N-Myc, was associated with poor prognosis in endometrioid EOC (P = .001) as well as in a subset of serous EOC with a high-MYCN profile (C5/proliferative; P = .019). Transient siRNA-mediated suppression of MRP4 in EOC cells led to reduced growth, migration and invasion, with the effects being most pronounced in endometrioid and C5-like serous cells compared to non-C5 serous EOC cells. Sustained knockdown of MRP4 also sensitized endometrioid cells to MRP4 substrate drugs. Furthermore, suppression of MRP4 decreased the growth of patient-derived EOC cells in vivo. Together, our findings provide the first evidence that MRP4 plays an important role in the biology of Myc-associated ovarian tumors and highlight this transporter as a potential therapeutic target for EOC