High-speed observation of sprite streamers

This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.Sprites are optical emissions in the mesosphere mainly at altitudes 50–90 km. They are caused by the sudden re-distribution of charge due to lightning in the troposphere which can produce electric fields in the mesosphere in excess of the local breakdown field. The resulting optical displays can be spectacular and this has led to research into the physics and chemistry involved. Imaging at faster than 5,000 frames per second has revealed streamer discharges to be an important and very dynamic part of sprites, and this paper will review high-speed observations of sprite streamers. Streamers are initiated in the 65–85 km altitude range and observed to propagate both down and up at velocities normally in the 106–5 9 107 m/s range. Sprite streamer heads are small, typically less than a few hundreds of meters, but very bright and appear in images much like stars with signals up to that expected of a magnitude -6 star. Many details of streamer formation have been modeled and successfully compared with observations. Streamers frequently split into multiple sub-streamers. The splitting is very fast. To resolve details will require framing rates higher than the maximum 32,000 fps used so far. Sprite streamers are similar to streamers observed in the laboratory and, although many features appear to obey simple scaling laws, recent work indicates that there are limits to the scaling.Research funding has been provided by the US National Science Foundation grants to the University of Alaska Fairbanks, and the US Air Force Academy, and by DARPA through a grant to the University of Florida

The Hall current system revealed as a statistical significant pattern during fast flows

We have examined the dawn-dusk component of the magnetic field, <I>B<sub>Y</sub></I>, in the night side current sheet during fast flows in the neutral sheet. 237 h of Cluster data from the plasma sheet between 2 August 2002 and 2 October 2002 have been analysed. The spatial pattern of <I>B<sub>Y</sub></I> as a function of the distance from the centre of the current sheet has been estimated by using a Harris current sheet model. We have used the average slopes of these patterns to estimate earthward and tailward currents. For earthward fast flows there is a tailward current in the inner central plasma sheet and an earthward current in the outer central plasma sheet on average. For tailward fast flows the currents are oppositely directed. These observations are interpreted as signatures of Hall currents in the reconnection region or as field aligned currents which are connected with these currents. Although fast flows often are associated with a dawn-dusk current wedge, we believe that we have managed to filter out such currents from our statistical patterns

Analysis of High-Throughput Flow Cytometry Data Using plateCore

Flow cytometry (FCM) software packages from R/Bioconductor, such as flowCore and flowViz, serve as an open platform for development of new analysis tools and methods. We created plateCore, a new package that extends the functionality in these core packages to enable automated negative control-based gating and make the processing and analysis of plate-based data sets from high-throughput FCM screening experiments easier. plateCore was used to analyze data from a BD FACS CAP screening experiment where five Peripheral Blood Mononucleocyte Cell (PBMC) samples were assayed for 189 different human cell surface markers. This same data set was also manually analyzed by a cytometry expert using the FlowJo data analysis software package (TreeStar, USA). We show that the expression values for markers characterized using the automated approach in plateCore are in good agreement with those from FlowJo, and that using plateCore allows for more reproducible analyses of FCM screening data

Development of a Canons of Practice Policy at Washington State University

Public policy educators, researchers, and administrators at Washington State University developed the Canons of Practice to guide faculty and staff engaging in contentious public issues. The need for such a document became evident when existing university policies and procedures lacked a suitable mechanism for resolving external criticism of public policy education and research. The Canons of Practice sets parameters for involvement in public policy research and education, provides guidelines for faculty and staff conduct, defines expectations of citizens and stakeholders, and establishes due process as the core of administrative response

Energy conversion at the Earth's magnetopause using single and multispacecraft methods

We present a small statistical data set, where we investigate energy conversion at the magnetopause using Cluster measurements of magnetopause crossings. The Cluster observations of magnetic field, plasma velocity, current density and magnetopause orientation are needed to infer the energy conversion at the magnetopause. These parameters can be inferred either from accurate multispacecraft methods, or by using single-spacecraft methods. Our final aim is a large statistical study, for which only single-spacecraft methods can be applied. The Cluster mission provides an opportunity to examine and validate single-spacecraft methods against the multispacecraft methods. For single-spacecraft methods, we use the Generic Residue Analysis (GRA) and a standard one-dimensional current density method using magnetic field measurements. For multispacecraft methods, we use triangulation (Constant Velocity Approach - CVA) and the curlometer technique. We find that in some cases the single-spacecraft methods yield a different sign for the energy conversion than compared to the multispacecraft methods. These sign ambiguities arise from the orientation of the magnetopause, choosing the interval to be analyzed, large normal current and time offset of the current density inferred from the two methods. By using the Finnish Meteorological Institute global MHD simulation GUMICS-4, we are able to determine which sign is likely to be correct, introducing an opportunity to correct the ambiguous energy conversion values. After correcting the few ambiguous cases, we find that the energy conversion estimated from single-spacecraft methods is generally lower by 70% compared to the multispacecraft methods.Peer reviewe

Cluster observations of a field aligned current at the dawn flank of a bursty bulk flow

This article describes observations of a bursty bulk flow (BBF) in the outer central plasma sheet. The observations are made with the Cluster satellites, located approximately 19 <I>R<sub>E</sub></I> downtail, close to the midnight sector in the Southern Hemisphere. 40–60 s after Cluster first detected the BBF, there was a large bipolar perturbation in the magnetic field. A Grad-Shafranov reconstruction has revealed that this is created by a field-aligned current at the flank of the BBF. Further analysis of the plasma moments has shown that the BBF has the properties of a depleted flux tube. Depleted flux tubes are an important theoretical model for how plasma and magnetic flux can be transported Earthward in the magnetotail as part of the Dungey cycle. The field aligned current is directed Earthward and is located at the dawn side of the BBF. Thus, it is consistent with the magnetic shear at the flank of an Earthward moving BBF. The total current has been estimated to be about 0.1 MA

Plasma convection in the magnetotail lobes: statistical results from Cluster EDI measurements

A major part of the plasma in the Earth's magnetotail is populated through transport of plasma from the solar wind via the magnetotail lobes. In this paper, we present a statistical study of plasma convection in the lobes for different directions of the interplanetary magnetic field and for different geomagnetic disturbance levels. The data set used in this study consists of roughly 340 000 one-minute vector measurements of the plasma convection from the Cluster Electron Drift Instrument (EDI) obtained during the period February 2001 to June 2007. The results show that both convection magnitude and direction are largely controlled by the interplanetary magnetic field (IMF). For a southward IMF, there is a strong convection towards the central plasma sheet with convection velocities around 10 km s<sup>−1</sup>. During periods of northward IMF, the lobe convection is almost stagnant. A <I>B<sub>y</sub></I> dominated IMF causes a rotation of the convection patterns in the tail with an oppositely directed dawn-dusk component of the convection for the northern and southern lobe. Our results also show that there is an overall persistent duskward component, which is most likely a result of conductivity gradients in the footpoints of the magnetic field lines in the ionosphere

flowCore: a Bioconductor package for high throughput flow cytometry

<p>Abstract</p> <p>Background</p> <p>Recent advances in automation technologies have enabled the use of flow cytometry for high throughput screening, generating large complex data sets often in clinical trials or drug discovery settings. However, data management and data analysis methods have not advanced sufficiently far from the initial small-scale studies to support modeling in the presence of multiple covariates.</p> <p>Results</p> <p>We developed a set of flexible open source computational tools in the R package flowCore to facilitate the analysis of these complex data. A key component of which is having suitable data structures that support the application of similar operations to a collection of samples or a clinical cohort. In addition, our software constitutes a shared and extensible research platform that enables collaboration between bioinformaticians, computer scientists, statisticians, biologists and clinicians. This platform will foster the development of novel analytic methods for flow cytometry.</p> <p>Conclusion</p> <p>The software has been applied in the analysis of various data sets and its data structures have proven to be highly efficient in capturing and organizing the analytic work flow. Finally, a number of additional Bioconductor packages successfully build on the infrastructure provided by flowCore, open new avenues for flow data analysis.</p