Pleurodema tucumanum: Predation

Herein we report predation of a juvenile Pleurodema tucumanum by the water bug Belostoma discretum. This observation took place during the night of 9 April 2009 in the vicinity of the Rio Claro (32.613°S, 66.139°W), near San Francisco, Ayacucho, San Luis 5570, Argentina, in a large pool enclosed by rocks at the river’s edge. A juvenile P. tucumanum (19.6 mm SVL) was observed floating on the surface of the pool (no deeper than 0.2 m), with a B. discretum (24.8 mm long) attached to its abdomen. The water bug was holding the frog with its forelegs while piercing the frog’s left hindlimb with its proboscis. At regular intervals the water bug swam with apparent difficulty carrying the frog to the bottom of the pool, where it rested for an instant before emerging for air and repeating these actions.Facultad de Ciencias Exacta

Pleurodema tucumanum: Predation

Herein we report predation of a juvenile Pleurodema tucumanum by the water bug Belostoma discretum. This observation took place during the night of 9 April 2009 in the vicinity of the Rio Claro (32.613°S, 66.139°W), near San Francisco, Ayacucho, San Luis 5570, Argentina, in a large pool enclosed by rocks at the river’s edge. A juvenile P. tucumanum (19.6 mm SVL) was observed floating on the surface of the pool (no deeper than 0.2 m), with a B. discretum (24.8 mm long) attached to its abdomen. The water bug was holding the frog with its forelegs while piercing the frog’s left hindlimb with its proboscis. At regular intervals the water bug swam with apparent difficulty carrying the frog to the bottom of the pool, where it rested for an instant before emerging for air and repeating these actions.Facultad de Ciencias Exacta

Characterization of the cytotoxic effects of the combination of cisplatin and flavanol (-)-epicatechin on human lung cancer cell line A549. An isobolographic approach

Background: Among malignancies, lung cancer is a leading cause of death. Platinum-based therapeutic compounds used to treat lung cancer have not been able to increase the survival of patients and such compounds have a high incidence of adverse and toxic effects. It has been proposed that flavonoids such as catechins may significantly reduce the risk of developing cancer, alongside with other health benefits. The aim of this work was to determine the effect of (-)-epicatechin, the main flavanol found in cocoa, on the proliferation of the lung non-small cell adenocarcinoma cancer cell line A549, and to determine its effects when added simultaneously with cisplatin. Materials and Methods: Concentration-response curves for cisplatin and epicatechin were obtained, inhibitory concentrations calculated and an isobolographic analysis was then performed. Results: We found that epicatechin has a concentration-dependent inhibitory effect on proliferation of tumor cells and the isobolographic analysis reveals that the effect of its combination with cisplatin is synergistic. It was also observed that epicatechin promotes cell death by apoptosis. Conclusions: Epicatechin might be considered for future studies to explore its possible use as coadjuvant in cisplatin-based treatments

Increased hepatic insulin sensitivity in mice lacking inhibitory leptin receptor signals

Leptin regulates food intake and energy expenditure by activating the long form of the leptin receptor (LepRb). Leptin also regulates glucose homeostasis by improving whole-body insulin sensitivity, but the mechanism remains undefined. Leptin action is mediated by phosphorylation of several tyrosine residues on LepRb. LepRb-Tyr985 plays an important role in the attenuation of LepRb signaling. We determined the contribution of LepRb-Tyr985-mediated signals to leptin action on insulin sensitivity using LepRb-Tyr985 mutant mice (l/l mice). Glucose tolerance and whole-body insulin-mediated glucose utilization were determined in wild-type (+/+) and l/l mice. Glucose tolerance was unaltered between female +/+ and l/l mice but enhanced in the male l/l mice. Serum insulin concentration was decreased at baseline and 15 min after a glucose injection in female l/l vs. +/+ mice (P < 0.05) but unaltered in the male l/l mice. However, basal and insulin-stimulated glucose transport in isolated soleus and extensor digitorum longus muscle was similar between +/+ and l/l mice, indicating skeletal muscle insulin sensitivity in vitro was not enhanced. Moreover, euglycemic-hyperinsulinemic clamps reveal hepatic, rather than peripheral, insulin sensitivity is enhanced in female l/l mice, whereas male l/l mice display both improved hepatic and peripheral insulin sensitivity. In conclusion, signals emanating from leptin receptor Tyr985 control hepatic insulin sensitivity in both female and male l/l mice. Lack of LepRb-Tyr985 signaling enhances whole-body insulin sensitivity partly through increased insulin action on the suppression of hepatic glucose production

A Multiple-Choice Maze-like Spatial Navigation Task for Humans Implemented in a Real-Space, Multipurpose Circular Arena

Spatial navigation is a key aspect of human behavior and it is still not completely understood. A number of experimental approaches exist, although most of the published data in the last decades have relied on virtual maze on-screen simulation or not-completely freely moving 3D devices. Some interesting recent developments, such as circular mazes, have contributed to analyze critical aspects of freely moving human spatial navigation in real space, although dedicated protocols only allow for simple approaches. Here, we have developed both specifically designed and home-assembled hardware equipment, and a customized protocol for spatial navigation evaluation in freely moving humans in a real space circular arena. The spatial navigation protocol poses an imitation of a real-space multiple-choice path maze with cul-de-sac and instances of non-linear movement. We have compared the results of this system to those of a number of validated, both virtual and real, spatial navigation tests in a group of participants. The system composed by hardware, the test protocol, and dedicated measure analysis designed in our laboratory allows us to evaluate human spatial navigation in a complex maze with a small and portable structure, yielding a highly flexible, adaptable, and versatile access to information about the subjects’ spatial navigation abilities

Mixed-method tutoring support improves learning outcomes of veterinary students in basic subjects

P. 1-10Tutoring is a useful tool in the university teaching-learning binomial, although its development is impaired in large classes. Recent improvements in information and communication technologies have made tutoring possible via the Internet. The aim of this study was to evaluate the efficacy of mixed-method academic tutoring in two basic subjects in Veterinary Science studies at the University of León (Spain) to optimize the usefulness of tutoring support in the college environment. This quasi-experimental study was firstly carried out as a pilot study in a small group of tutored students of “Cytology and Histology” (CH) (47/186; 25.3%) and “Veterinary Pharmacology” (VP) (33/141; 23.4%) subjects, and was implemented in a large class of CH the next academic year (150 students) while comparing the results with those obtained in a previous tutorless course (162 students). Tutored students were given access to online questionnaires with electronic feedback on each subject. In addition to traditional tutoring carried out in both tutored and tutorless students, the pilot study included three sessions of face-to-face tutoring in order to monitor the progress of students. Its efficacy was assessed by monitoring students’ examination scores and attendance as well as a satisfaction survey. Online tutoring support, together with conventional teaching methods, may be a useful method to incorporate student-centered learning in basic subjects in Veterinary Science.S

Is HCG31 undergoing a merger or a fly-by interaction?

We present Fabry-Perot and multi-object spectroscopy of the galaxies in Hickson compact group 31 (HCG31). Based upon our H$\alpha$ data cubes, galaxies A and C are a single entity, showing no discontinuity in their kinematics. Kinematically, galaxy E is probably a component of the A+C complex; otherwise it is a recently detached fragment. Galaxy F appears, both kinematically and chemically, to have formed from material tidally removed from the A+C complex. Galaxies B and G are kinematically distinct from this complex. Galaxy Q also has a radial velocity compatible with group membership. Galaxies A, B, C, and F have nearly identical oxygen abundances, despite spanning a luminosity range of 5$$mag. Galaxy B's oxygen abundance is normal for its luminosity, while galaxy F's abundance is that expected given its origin as a tidal fragment of the A+C complex. The oxygen abundances in galaxies A and C are also understandable if the A+C complex is a late-type spiral suffering strong gas inflow and star formation as a result of a tidal interaction. Given the kinematics of both the galaxies and the \ion{H}{i} gas, the oxygen abundances, and the position of galaxy G, we propose that an interaction of galaxy G with the A+C complex, rather than a merger of galaxies A and C, is a more complete explanation for the tidal features and other properties of HCG31. In this case, the A+C complex need not be a merger in progress, though this is not ruled out.Comment: accepted for publication by Astronomy & Astrophysic

Effectiveness, safety/tolerability of OBV/PTV/r ± DSV in patients with HCV genotype 1 or 4 with/without HIV-1 co-infection, chronic kidney disease (CKD) stage IIIb/V and dialysis in Spanish clinical practice – preliminary data Vie-KinD study

Poster presentatio

Interventions in health organisations to reduce the impact of adverse events in second and third victims

Background Adverse events (AE) are also the cause of suffering in health professionals involved. This study was designed to identify and analyse organization-level strategies adopted in both primary care and hospitals in Spain to address the impact of serious AE on second and third victims. Methods A cross-sectional study was conducted in healthcare organizations assessing: safety culture; health organization crisis management plans for serious AE; actions planned to ensure transparency in communication with patients (and relatives) who experience an AE; support for second victims; and protective measures to safeguard the institution’s reputation (the third victim). Results A total of 406 managers and patient safety coordinators replied to the survey. Deficient provision of support for second victims was acknowledged by 71 and 61 % of the participants from hospitals and primary care respectively; these respondents reported there was no support protocol for second victims in place in their organizations. Regarding third victim initiatives, 35 % of hospital and 43 % of primary care professionals indicated no crisis management plan for serious AE existed in their organization, and in the case of primary care, there was no crisis committee in 34 % of cases. The degree of implementation of second and third victim support interventions was perceived to be greater in hospitals (mean 14.1, SD 3.5) than in primary care (mean 11.8, SD 3.1) (p?<?0.001). Conclusions Many Spanish health organizations do not have a second and third victim support or a crisis management plan in place to respond to serious AEs