Stercoraceous perforation, rare but potentially fatal cause: case report

Stercoraceous perforation is an infrequent pathology but with high mortality rates, it is increasingly recognized as a consequence of chronic constipation, it occurs in elderly people, the diagnosis is a surgical challenge, sudden abdominal pain is the initial symptom associated with signs of peritoneal irritation and the diagnostic study of choice is tomography, the treatment of choice is early surgery, the prognosis is related to the patient's comorbidities. We present the clinical case and review of the subject of a 72-year-old woman who presented stercoraceous perforation secondary to chronic constipation, emergency surgery was performed with resection of the affected bowel, colostomy and Hartmann's pouch with favorable short-term results

Fluorescent Imprinted Nanoparticles for Sensing of Chlorogenic Acid in Coffee Extracts

Green coffee beans are particularly rich in chlorogenic acids (CGAs), and their identification and quantification are usually performed by HPLC, coupled with mass spectrometry (LC-MS). Although there are a few examples of molecularly imprinted polymers (MIPs) for chlorogenic acid (5-CQA) recognition present in the literature, none of them are based on optical fluorescence, which is very interesting given its great sensitivity. In the present manuscript, fluorescent polymeric imprinted nanoparticles were synthetized following the non-covalent approach using hydrogenated 5-O-caffeoylquinic acid (H-5-CQA) as the template. The capability of the polymer to bind 5-CQA was evaluated by HPLC and fluorescence. A real sample of coffee extract was also analyzed to verify the selectivity of the polymer. Polymer fMIP01, containing 4-vinylpyridine and a naphtalimide derivative as monomers, showed a good response to the fluorescence quenching in the range 39 mu M-80 mM. In the real sample, fMIP01 was able to selectively bind 5-CQA, while caffeine was not recognized. To demonstrate this, there is a promising system that can be exploited in the design of an optical sensor for 5-CQA detection. Polymer fMIP01 was immobilized by physical entrapment on a functionalized glass surface, showing a quenching of fluorescence with an increase of the CGA concentration between 156 mu M and 40 mM

Marcadores moleculares y morfológicos para la descripción de variabilidad en caña de azúcar (Saccharum officiarum) con fines de manejo y conservación de germoplasma

La caña de azúcar es uno de los cultivos industriales más importantes de regiones tropicales y subtropicales. El INTA (Argentina) administra un Banco de Germoplasma de caña de azúcar y lleva a cabo un programa de mejora. El presente trabajo fue diseñado para estimar la variabilidad fenotípica y genética entre 65 accesiones de caña de azúcar seleccionadas del INTA. Se aplicaron métodos de clasificación y ordenamiento en el análisis de datos morfológicos y de SSR. EL Análisis de Procrustes Generalizado permitió evaluar la correlación entre las relaciones establecidas a partir de ambos tipos de marcadores. Un buen ajuste entre los dendrogramas y las matrices de similitud fue soportado por un alto coeficiente de correlación cofenética (r=0,82, p<0,0001; r=0,73, p<0,0001; r=0,82, p<0,0001 para datos cuantitativos, cualitativos y moleculares respectivamente). La presencia de una estructura poblacional fue reconocida cuando se consideraron los diferentes tipos de datos. El Procrustes permitió detectar aquellas accesiones que serían responsables de la baja correlación detectada entre configuraciones individuales (73%). Tanto los marcadores morfológicos como los moleculares resultaron lo suficientemente discriminativos para diferenciar accesiones. No obstante, no fue posible correlacionar las asociaciones establecidas por los marcadores con el origen de los materiales. Las distancias fenotípicas y genéticas basadas en información morfológica y molecular será de utilidad para asistir en la conservación y organización de los materiales de la colección y elegir combinaciones parentales con propósito de mejora.Sugarcane is one of the most important industrial crops in tropical and subtropical regions. INTA (Argentina) administrates a Sugarcane Germplasm Bank and carries out a breeding program. The current study was designed to assess the phenotypic and genetic diversity among 65 sugarcane accessions selected from the INTA. Clustering and ordination methods based on quantitative and qualitative morphological traits and SSR data were applied. Generalized Procrustes Analysis allowed evaluating the correlation between relationships established with both markersA good of fit between dendrograms and similarity matrices were revealed by high cophenetic coefficients (r=0.82, p<0.0001; r=0.73, p<0.0001; r=0.82, p<0.0001 for phenotypic quantitative, phenotypic qualitative and molecular data respectively). The presence of different reliable population structure was observed when considering different data sources. Procrustes allowed finding those accessions that should have been responsible for the low correlation found between the individual configurations (73%). Both morphologic and molecular markers resulted discriminative enough to differentiate among accessions. It was not possible, however, to correlate associations of markers with the origin of materials. Phenotypic and genetic distances based on morphology and molecular information serves to assist conservation and organization of collection of materials, and the choice of parent combinations for breeding purposes.Fil: Pocovi, Mariana Inés. Universidad Nacional de Salta. Facultad de Ciencias Naturales; ArgentinaFil: Collavino, Norma Graciela. Universidad Nacional de Salta. Facultad de Ciencias Naturales; ArgentinaFil: Gutiérrez, Angela Verónica. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; ArgentinaFil: Taboada, Gisel María. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; ArgentinaFil: Castillo, Veronica. Universidad Nacional de Salta. Facultad de Ciencias Naturales; ArgentinaFil: Delgado, Romina Paola. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; ArgentinaFil: Mariotti, Jorge. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentin

Expression of the HPV18/E6 oncoprotein induces DNA damage

Abstract This study investigated possible variations in DNA damage in HeLa cells with silenced expression of the HPV/E6oncogene compared with HeLa cells with normal expression of the E6oncogene using the DNA breakage detection-fluorescence in situ hybridization (DBD-FISH) technique and a whole human genome DNA probe. The variable levels of DNA breaks present were measured quantitatively using image analysis after whole-genome DNA hybridization. HeLa cells with silenced expression of the HPV18/E6 oncogene showed a significant decrease in DNA damage compared with parental cells with normal expression of the E6oncogene. These results were confirmed by alkaline comet assay. In conclusion, we demonstrated a decrease in DNA damage in HeLa clones associated with low expression of the HPV/E6 oncogene

Evaluación del cloruro de calcio a diferentes concentraciones en poscosecha de guayaba (Psidium guajava L.) con diferentes temperaturas

La búsqueda de estrategias que permitan alargar el tiempo de vida útil en frutas ha tomado gran importancia en la industria alimenticia, ya que esto evita perdidas en la producción. En la presente investigación, se evaluó el efecto del cloruro de calcio a tres diferentes concentraciones en la conservación de la guayaba (Psidium guajava L.) expuesta a tres diferentes temperaturas. Las propiedades fisicoquímicas fueron evaluadas durante 21 días, con el fin de alargar la vida poscosecha de la guayaba conservando sus propiedades nutrimentales. Los resultados indican, que es posible incrementar la vida útil de las guayabas aplicando cloruro de calcio. La aplicación de los tratamientos no modifica el valor nutricional de los frutos examinados, mientras el análisis sensorial mostro una gran diferencia en comparación a los frutos sin tratamiento. Por lo tanto, es posible que aplicaciones de cloruro de calcio sean tomadas en cuenta para recubrir guayabas y aumentar su vida útil.The search for strategies to extend the shelf life in fruits has taken great importance in the food industry, as this prevents production losses. In the present investigation, the effect of calcium chloride at three different concentrations on the conservation of guava (Psidium guajava L.) exposed to three different temperatures was evaluated. The physicochemical properties were evaluated for 21 days, in order to extend the post-harvest life of the guava while preserving its nutritional properties. The results indicate that it is possible to increase the shelf life of guavas by applying calcium chloride. The application of the treatments does not modify the nutritional value of the examined fruits, while the sensory analysis showed a great difference compared to the fruits without treatment. Therefore, it is possible that calcium chloride applications are taken into account to coat guavas and increase their shelf life

Applied research to new construction materials subject

[EN] The degree in Building Engineering at the University of Burgos has been included the Bologna System 10 years ago, implemented simultaneously in the 2010-2011 academic year the entire degree - four courses. According with the initial strategies, the acquisition of competences through project research work in the construction materials subjects has been one of the elements developed and consolidated, especially in the elective subjects of higher courses where training of the student already allows this type of resources. However, the work begins the first year with specific classes to introduce the investigation within the subjects of construction materials, with agenda reflected in the educational guide. One of them, for example, is an intensive course of advanced searches in international scientific databases, which must be used in a practical way to test what has been learned, and which can be extrapolated to the rest of the subjects of the degree.[ES] El grado en Arquitectura Técnica de la Universidad de Burgos lleva un recorrido de 10 años en el Plan Bolonia, que implantó de manera simultánea en el curso 2010-2011 para los cuatro cursos del grado. Dentro de las estrategias iniciales del Grado, la adquisición de competencias a través del trabajo por proyectos en las asignaturas de materiales de construcción ha sido uno de los factores que se ha desarrollado y afianzado, especialmente en la asignaturas optativas de cursos más altos donde la formación del estudiante ya permite este tipo de recursos. No obstante, se comienza desde primer curso con clases específicas de introducción a la investigación dentro de las asignaturas de materiales de construcción, con temario contemplado en la guía docente. Una de ellas, por ejemplo, es un curso intensivo de búsquedas avanzadas en bases de datos científicas internacionales, que debe ser utilizado de manera práctica para poner a prueba lo aprendido, y que puede ser extrapolado al resto de asignaturas de la titulación.Gadea Sáinz, J.; Calderón Carpintero, V.; Junco Petrement, C.; Gutiérrez-González, S.; Rodríguez Saiz, Á.; Garabito López, J. (2021). Investigación aplicada a la formación en nuevos materiales de construcción. Editorial Universitat Politècnica de València. 37-43. https://doi.org/10.4995/EDIFICATE2021.2021.13531OCS374

Enhanced hemato-endothelial specification during human embryonic differentiation through developmental cooperation between AF4-MLL and MLL-AF4 fusions.

The t(4;11)(q21;q23) translocation is associated with high-risk infant pro-B-cell acute lymphoblastic leukemia and arises prenatally during embryonic/fetal hematopoiesis. The developmental/pathogenic contribution of the t(4;11)-resulting MLL-AF4 (MA4) and AF4-MLL (A4M) fusions remains unclear; MA4 is always expressed in patients with t(4;11)+ B-cell acute lymphoblastic leukemia, but the reciprocal fusion A4M is expressed in only half of the patients. Because prenatal leukemogenesis manifests as impaired early hematopoietic differentiation, we took advantage of well-established human embryonic stem cell-based hematopoietic differentiation models to study whether the A4M fusion cooperates with MA4 during early human hematopoietic development. Co-expression of A4M and MA4 strongly promoted the emergence of hemato-endothelial precursors, both endothelial- and hemogenic-primed. Double fusion-expressing hemato-endothelial precursors specified into significantly higher numbers of both hematopoietic and endothelial-committed cells, irrespective of the differentiation protocol used and without hijacking survival/proliferation. Functional analysis of differentially expressed genes and differentially enriched H3K79me3 genomic regions by RNA-sequencing and H3K79me3 chromatin immunoprecipitation-sequencing, respectively, confirmed a hematopoietic/endothelial cell differentiation signature in double fusion-expressing hemato-endothelial precursors. Importantly, chromatin immunoprecipitation-sequencing analysis revealed a significant enrichment of H3K79 methylated regions specifically associated with HOX-A cluster genes in double fusion-expressing differentiating hematopoietic cells. Overall, these results establish a functional and molecular cooperation between MA4 and A4M fusions during human hematopoietic development.Wellcome Trust, CRUK, Bloodwise, ERC, Generalitat de Catalunya, Spanish Ministry of Economy and Competitiveness, Spanish Association Against cancer, Health Institute Carlos III, NIHR GOSH BRC, Great Ormond Steet Hospital Children's Charity, Deutsche José Carreras Leukämie Stiftung, Obra Social La Caixa-Fundaciò Josep Carreras, Spanish Association of Cancer Researc

Pandemic and Socio-Educational Strategies, Identification of the Proposals of the Spanish Case

Ante la situación de pandemia y alerta sanitaria, el Gobierno español decreta en marzo el estado de alarma para gestionar la situación de emergencia generada por la rápida propagación del COVID-19. Los efectos de este paquete de medidas afectan especialmente a los espacios de socialización, restringiendo el contacto, siendo la infancia y la adolescencia especialmente afectadas. El incremento de dinámicas familiares que ponen en riesgo a los menores ponen de manifiesto la necesidad de fortalecer el sistema de protección y prevención familiar. Se ha realizado un análisis de las principales medidas de prevención que se han llevado a cabo en España tanto a nivel autonómico como estatal. Los resultados han expuesto que buena parte de estas medidas son de carácter terciario, siendo el apoyo psicológico la medida más recurrente. En conclusión, se refleja la necesidad de aumentar las medidas de prevención primaria que reduzcan los riesgos infanto-juveniles a los que se ha expuesto a la infancia en el contexto familiar.In view of the pandemic and health alert situation, the Spanish Government decreed a state of alarm in March to manage the emergency situation generated by the rapid spread of COVID-19. The effects of this package of measures especially affect the spaces of socialization, restricting contact, with children and adolescents being especially affected. The increase in family dynamics that put minors at risk makes clear the need to strengthen the system of family protection and prevention. An analysis of the main prevention measures that have been carried out in Spain at both the regional and state level has been made. The results have shown that many of these measures are of a tertiary nature, with psychological support being the most recurrent measure. In conclusion, the need to increase primary prevention measures to reduce the risks to children and young people to which they have been exposed in the family context is reflecte

The U.S.-Mexico Border Infectious Disease Surveillance Project: Establishing Binational Border Surveillance

In 1997, the Centers for Disease Control and Prevention, the Mexican Secretariat of Health, and border health officials began the development of the Border Infectious Disease Surveillance (BIDS) project, a surveillance system for infectious diseases along the U.S.-Mexico border. During a 3-year period, a binational team implemented an active, sentinel surveillance system for hepatitis and febrile exanthems at 13 clinical sites. The network developed surveillance protocols, trained nine surveillance coordinators, established serologic testing at four Mexican border laboratories, and created agreements for data sharing and notification of selected diseases and outbreaks. BIDS facilitated investigations of dengue fever in Texas-Tamaulipas and measles in California–Baja California. BIDS demonstrates that a binational effort with local, state, and federal participation can create a regional surveillance system that crosses an international border. Reducing administrative, infrastructure, and political barriers to cross-border public health collaboration will enhance the effectiveness of disease prevention projects such as BIDS