144 research outputs found
A comparative clinical study on Tagara-Devadaru Lepa with and without Prachhanna in the management of Indralupta with special reference to Alopecia Areata
- Publication venue
- Maharshi Charaka Ayurveda Organization
- Publication date
- 31/10/2019
- Field of study
Hair disorders causes negative impact towards individual and his/her quality of life. Indralupta is a disease in which hair is lost from some or all areas of the body, usually from scalp. It shows one or more round spot on the scalp . It arises from the vitiation of Tridosha and Rakta. Treatment advised is Siravedhana and topical application of herbal and mineral drugs. To avoid complications of Siravedhana, inthis study Prachhanna was preffered and Tagara-Devadaru chosen as drug for topical application at the site of Indralupta. In this study we had taken 40 Diagnosed patients of Indraluptaand were subjected to clinical trials. They were randomly assigned into two Groups namely Group A and Group B. Group A treated with Tagara-Devadaru Lepa, while subject under Group B treated by Prachhanna along with Tagara-Devadaru Lepa. The treatment modalities of TagaraDevadaru Lepa and Prachhanna with Tagara-Devadaru Lepa are equally efficacious in treating.On comparing the results of Group A and Group B, the conclusion were drawn.Both the methods of treatment are cost effective, easy to prepare and had no adverse effects
Genetic variability of the bollworm, Helicoverpa armigera, occurring on different host plants
- Author
- Publication venue
- University of Wisconsin Library
- Publication date
- Field of study
The bollworm, Helicoverpa armigera Hübner (Lepidoptera: Noctuidae) is a polyphagous pest of worldwide occurrence inflicting annual crop damage in India worth US$ 1billion. In India this insect occurs as a major pest in many economically important crops, including cotton, pigeonpea, chickpea, tomato, okra, and blackgram. Understanding the genetic variation among the H. armigera populations occurring on host plants has become essential to understand the variation in their susceptibility to different insecticides, including Bacillus thuringiensis. This preliminary study uses 10 microsatellite simple sequence repeat (SSR) markers, to provide insight into the genetic variability of H. armigera populations from six different host plants. Nine of the SSR primers indicated high variability across the different host associated populations with polymorphism ranging from 75 to 100 per cent. Using the un-weighted pair-group method analysis, H. armigera collected and reared from cotton stood out as unique in one cluster while the insects collected and reared on all other hosts grouped separately
Holocene evolution of the Xagó dune field (Asturias, NW Spain) reconstructed by means of morphological mapping and ground penetrating radar surveys
- Author
- A Alonso
- A Cearreta
- A Cearreta
- A Feal-Pérez
- A Feal-Pérez
- A García-Artola
- A Martínez Cortizas
- A Martínez Graña
- A Neal
- AD Short
- AD Short
- AR Gujar
- CS Bristow
- CS Bristow
- CS Bristow
- CS Bristow
- CS Bristow
- D Gómez-Ortiz
- D Kim
- D Moura
- D. Rubio-Melendi
- D. W. T. Jackson
- DM Rubin
- E Leorri
- F García-Novo
- F González Taboada
- G Chust
- G Flor
- G Flor
- G Flor
- G Flor
- G Flor
- G Flor
- G Flor-Blanco
- G Iglesias
- G Mary
- G. Flor
- G. Flor-Blanco
- I Rodríguez Santalla
- I Suárez Ruiz
- IV Buynevich
- IV Buynevich
- J Araújo-Gomes
- J Bigarella
- J García-Albá
- J Gracia
- J. P. Fernández-Álvarez
- JA Rodríguez-Asensio
- JD Girardi
- JM Edeso
- K Pye
- KG Havholm
- L Nielsen
- LB Clemmensen
- M Hoyos
- MAJ Bakker
- MH Ruz
- NP Psuty
- PA Hesp
- PA Hesp
- PA Hesp
- PA Hesp
- R Danielsen
- R González-Villanueva
- R González-Villanueva
- R Ramos
- RA Davis Jr
- RG Davidson-Arnott
- RL Dam Van
- RL Dam Van
- RWG Carter
- S Costas
- S Heteren Van
- SP Leatherman
- TA Łabuz
- TG Fisher
- WF Tanner
- WS Cooper
- Y Lee
- Z Harari
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- 01/01/2015
- Field of study
NBAS variants are associated with quantitative and qualitative NK and B cell deficiency
- Author
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- 01/01/2021
- Field of study
Purpose
Biallelic pathogenic NBAS variants manifest as a multisystem disorder with heterogeneous clinical phenotypes such as recurrent acute liver failure, growth retardation, and susceptibility to infections. This study explores how NBAS-associated disease affects cells of the innate and adaptive immune system.
Methods
Clinical and laboratory parameters were combined with functional multi-parametric immunophenotyping methods in fifteen NBAS-deficient patients to discover possible alterations in their immune system.
Results
Our study revealed reduced absolute numbers of mature CD56dim natural killer (NK) cells. Notably, the residual NK cell population in NBAS-deficient patients exerted a lower potential for activation and degranulation in response to K562 target cells, suggesting an NK cell–intrinsic role for NBAS in the release of cytotoxic granules. NBAS-deficient NK cell activation and degranulation was normalized upon pre-activation by IL-2 in vitro, suggesting that functional impairment was reversible. In addition, we observed a reduced number of naïve B cells in the peripheral blood associated with hypogammaglobulinemia.
Conclusion
In summary, we demonstrate that pathogenic biallelic variants in NBAS are associated with dysfunctional NK cells as well as impaired adaptive humoral immunity
Inhibition of STAT3 signaling as critical molecular event in resveratrol-suppressed ovarian cancer cells
- Author
- A Davis
- A Gąsowska-Bodnar
- A Sato
- A Suri
- A Usui
- AL Widlund
- C Wu
- D Vergara
- DC Bodurka
- F Collinson
- G Aravantinos
- GN Zhang
- H Piotrowska
- H Thomadaki
- Hong Li
- J Satoh
- J Stewart
- JH Kang
- Jia Liu
- JM Dann
- JM Smoliga
- JW Groeneweg
- K Mujoo
- KA Lowe
- KB Harikumar
- L Bodnar
- L Galluzzi
- L Lydiksen
- Li-Xia Zhong
- LJ Yu
- M Basu
- M Signorelli
- M Takai
- Ming-Jun Zhong
- ML Wu
- Mo-Li Wu
- N Husseinzadeh
- N Katsumata
- NS Anderson
- P Yue
- P Zhang
- P Zhang
- Q Wang
- RB Hazan
- RL Baldwin
- RN Buick
- S Gujar
- S Tsunetoh
- SP Tenbaum
- T Kamisha
- T Reya
- TC Hamilton
- XH Shu
- XH Shu
- XH Shu
- Xiao-Yan Chen
- Xiao-Yu Liu
- Y Rezk
- Yang Zhang
- YW Kim
- Z Sun
- ZL Yuan
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- Field of study
Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)
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- A. -G. Wu
- A. C. Ma
- A. K. Au
- Abdel-Aziz A. K.
- Abdelfatah S.
- Abdellatif M.
- Abdoli A.
- Abel S.
- Abeliovich H.
- Abildgaard M. H.
- Abudu Y. P.
- Acevedo-Arozena A.
- Adamopoulos I. E.
- Adeli K.
- Adolph T. E.
- Adornetto A.
- Aflaki E.
- Agam G.
- Agarwal A.
- Aggarwal B. B.
- Agnello M.
- Agostinis P.
- Agrewala J. N.
- Agrotis A.
- Aguilar P. V.
- Ahmad S. T.
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- Ahumada-Castro U.
- Aits S.
- Aizawa S.
- Akkoc Y.
- Akoumianaki T.
- Akpinar H. A.
- Al-Abd A. M.
- Al-Akra L.
- Al-Gharaibeh A.
- Alaoui-Jamali M. A.
- Alberti S.
- Alcocer-Gomez E.
- Alessandri C.
- Ali M.
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- Aliwaini S.
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- Zarebkohan A.
- Zarrouk A.
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- Zhan L.
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- Zorov D. B.
- Zorzano A.
- Zou W.
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- Zwerschke W.
- Publication venue
- 'Informa UK Limited'
- Publication date
- 01/01/2021
- Field of study
Molecular characterization of occult hepatitis B virus infection in patients with end-stage liver disease in Colombia.
- Author
- A Duque-Jaramillo
- A Fernandez-Medarde
- A Marzano
- AT Hall
- B Abedi-Ardekani
- B Seigneres
- C Brechot
- C Gerstenkorn
- CH Huang
- CK Cheung
- CS Coffin
- D di Filippo Villa
- D Rios
- DA Gouas
- DK Wong
- E Michailidis
- E Tabor
- EA Santos
- F Birnbaum
- F Cortes-Mancera
- F Zoulim
- Fabian Cortes-Mancera
- FV Chisari
- G Raimondo
- G Raimondo
- G Raimondo
- G Squadrito
- G Zhong
- GB Zeng
- GC Kimbi
- GM Barros Junior
- GY Minuk
- H Bautista
- H Bautista-Amorocho
- H Kim
- H Kim
- HL Chan
- HL Zhu
- I Baginski
- I Cacciola
- I Pult
- IC Ramirez
- Isabelle Chemin
- J Arroyave
- J Kock
- J Lucifora
- J Samal
- J Torresi
- JH Ireland
- JH Kao
- JI Tsui
- Juan Carlos Restrepo-Gutierrez
- Julio Cesar Rendon
- K Koike
- K Shetty
- K Tamura
- KQ Hu
- L Belloni
- L Menendez-Arias
- M Beltran
- M Bottecchia
- M Devesa
- M Di Stefano
- M Hofer
- M Krajden
- M Levrero
- M Minami
- M Nassal
- M Nunez
- Maria-Cristina Navas
- MI Allen
- MV Alvarado Mora
- MV Alvarado Mora
- N Coppola
- N Freitas
- N Mahboobi
- NF Ma
- NK Tonks
- O Chazouilleres
- P Deny
- P Paterlini
- P Paterlini
- P Polo
- P Toniutto
- P Vivekanandan
- Q Dong
- R Kannangai
- R Lozano
- R Mazzolla
- RA Ponde
- RC Dickson
- RS Alencar
- S Frietze
- S Gunther
- S Jammeh
- S Ocana
- S Parekh
- S Schaefer
- S Takada
- S Tong
- SA Gujar
- Sergio Hoyos
- SI Strasser
- SJ Muñoz
- SM Wakil
- T Pollicino
- TC Ferrari
- TJ Liang
- TM De Feo
- V Ghisetti
- V Kumar
- WA Rios-Ocampo
- WK Sung
- X Hu
- Y Fang
- Y Murakami
- Y Niu
- Y Zhong
- YS Lee
- YZ Castellanos
- Z Han
- Z Zhang
- Z Zhang
- ZH Zhang
- ZK Hassan
- ZM Huang
- Publication venue
- 'Public Library of Science (PLoS)'
- Publication date
- 01/01/2017
- Field of study
ABSTARCT: Hepatitis B virus (HBV) occult infection (OBI) is a risk factor to be taken into account in transfusion, hemodialysis and organ transplantation. The aim of this study was to identify and characterize at the molecular level OBI cases in patients with end-stage liver disease.
METHODS:
Sixty-six liver samples were obtained from patients with diagnosis of end-stage liver disease submitted to liver transplantation in Medellin (North West, Colombia). Samples obtained from patients who were negative for the surface antigen of HBV (n = 50) were tested for viral DNA detection by nested PCR for ORFs S, C, and X and confirmed by Southern-Blot. OBI cases were analyzed by sequencing the viral genome to determine the genotype and mutations; additionally, viral genome integration events were examined by the Alu-PCR technique.
RESULTS:
In five cases out of 50 patients (10%) the criteria for OBI was confirmed. HBV genotype F (subgenotypes F1 and F3), genotype A and genotype D were characterized in liver samples. Three integration events in chromosomes 5q14.1, 16p13 and 20q12 affecting Receptor-type tyrosine-protein phosphatase T, Ras Protein Specific Guanine Nucleotide Releasing Factor 2, and the zinc finger 263 genes were identified in two OBI cases. Sequence analysis of the viral genome of the 5 OBI cases showed several punctual missense and nonsense mutations affecting ORFs S, P, Core and X.
CONCLUSIONS:
This is the first characterization of OBI in patients with end-stage liver disease in Colombia. The OBI cases were identified in patients with HCV infection or cryptogenic cirrhosis. The integration events (5q14.1, 16p13 and 20q12) described in this study have not been previously reported. Further studies are required to validate the role of mutations and integration events in OBI pathogenesis
Ruthenium oxide-carbon-based nanofiller-reinforced conducting polymer nanocomposites and their supercapacitor applications.
- Author
- A Vita
- AA Ensafi
- AN Naveen
- AY Lo
- B Brown
- B Das
- B Mortazavi
- BH Kim
- BH Kim
- BH Lei
- BZ Nikolic
- C Menna
- C Wei
- C Zhang
- C Zhang
- Carlos Fernandez
- CB Arnold
- CC Hu
- CC Hu
- CC Hu
- CC Hu
- CC Hu
- CC Hu
- CC Liu
- CC Yang
- CD Lokhande
- CJ Cho
- CN Chervin
- CY Jung
- D Xiang
- DD Zhao
- DM Guldi
- DP Dubal
- DP Nair
- DS Achilleos
- DWH Fam
- F Pico
- F Wang
- FZ Amir
- G Arabale
- G Lakshminarayana
- H Lee
- H Li
- H Murakami
- H Xia
- IE Rauda
- IH Kim
- IIK Yoon
- J Bae
- J Bouchard
- J Bouchard
- J Gopinathan
- J Liang
- JV Ryan
- JY Hwang
- K Chaitra
- K Chaitra
- K Naoi
- K Naoi
- KM Kim
- KS Yang
- L Chen
- L Khandare
- L Peng
- LW Fan
- LW Fan
- M Gnerlich
- M Liu
- M Ramani
- M Ramani
- M Yu
- M Zhi
- Murat Ates
- MW Chen
- N Sahan
- N Terasawa
- N Terasawa
- N Ullah
- NL Nquyen
- O Barbieri
- P Sekar
- P Wang
- P Wang
- P Xiong
- PO Park
- R Liu
- R Vellacheri
- R Warren
- RA Fisher
- RB Bi
- RB Rakhi
- RB Rakhi
- RC Ambare
- RJ Warzoha
- S Chen
- S Faraji
- S Makino
- S Neupane
- S Sopcic
- SC Hong
- T Brezesinski
- T Yousefi
- TM Ali
- TP Gujar
- US Shin
- V Augustyn
- V Borjanovic
- VD Patake
- VR Shinde
- VV Panic
- W Sugimoto
- W Sugimoto
- W Zubiao
- X Leng
- X Leng
- X Liu
- X Liu
- X Luo
- X Wang
- X Wang
- X Wu
- X Zhao
- XF Yang
- XL Guo
- XL Guo
- Y Meng
- Y Ni
- Y Shu
- Y Wang
- Y Yang
- Y Yao
- Y Zhang
- Y Zhu
- YG Wang
- YQ Shao
- YW Ju
- YX Wei
- Z Algharaibeh
- Z Chen
- Z Hu
- Z Wu
- Z Yao
- ZS Wu
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- 22/08/2018
- Field of study
In this review article, we have presented for the first time the new applications of supercapacitor technologies and working principles of the family of RuO2-carbon-based nanofiller-reinforced conducting polymer nanocomposites. Our review focuses on pseudocapacitors and symmetric and asymmetric supercapacitors. Over the last years, the supercapacitors as a new technology in energy storage systems have attracted more and more attention. They have some unique characteristics such as fast charge/discharge capability, high energy and power densities, and long stability. However, the need for economic, compatible, and easy synthesis materials for supercapacitors have led to the development of RuO2-carbon-based nanofiller-reinforced conducting polymer nanocomposites with RuO2. Therefore, the aim of this manuscript was to review RuO2-carbon-based nanofiller-reinforced conducting polymer nanocomposites with RuO2 over the last 17 years
Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)1.
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- Abdel-Aziz AK
- Abdelfatah S
- Abdellatif M
- Abdoli A
- Abel S
- Abeliovich H
- Abildgaard MH
- Abudu YP
- Acevedo-Arozena A
- Adamopoulos IE
- Adeli K
- Adolph TE
- Adornetto A
- Aflaki E
- Agam G
- Agarwal A
- Aggarwal BB
- Agnello M
- Agostinis P
- Agrewala JN
- Agrotis A
- Aguilar PV
- Ahmad ST
- Ahmed ZM
- Ahumada-Castro U
- Aits S
- Aizawa S
- Akkoc Y
- Akoumianaki T
- Akpinar HA
- Al-Abd AM
- Al-Akra L
- Al-Gharaibeh A
- Alaoui-Jamali MA
- Alberti S
- Alcocer-Gómez E
- Alessandri C
- Ali M
- Alim Al-Bari MA
- Aliwaini S
- Alizadeh J
- Almacellas E
- Almasan A
- Alonso A
- Alonso GD
- Altan-Bonnet N
- Altieri DC
- Alves da Costa C
- Alves S
- Alzaharna MM
- Amadio M
- Amantini C
- Amaral C
- Ambrosio S
- Amer AO
- Ammanathan V
- An Z
- Andersen SU
- Andrabi SA
- Andrade-Silva M
- Andres AM
- Angelini S
- Ann D
- Anozie UC
- Ansari MY
- Antas P
- Antebi A
- Antón Z
- Anwar T
- Apetoh L
- Apostolova N
- Araki T
- Araki Y
- Arasaki K
- Araya J
- Araújo WL
- Arden C
- Arguelles S
- Arias E
- Arikkath J
- Arimoto H
- Ariosa AR
- Armstrong-James D
- Arnauné-Pelloquin L
- Aroca A
- Arroyo DS
- Arsov I
- Artero R
- Arévalo M-A
- Asaro DML
- Aschner M
- Ashrafizadeh M
- Ashur-Fabian O
- Atanasov AG
- Au AK
- Auberger P
- Auner HW
- Aurelian L
- Autelli R
- Avagliano L
- Aveic S
- Aveleira CA
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- Ayton S
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- Baksi S
- Balazadeh S
- Balbi T
- Baldari CT
- Balduini W
- Ballabio A
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- Balzan R
- Bandopadhyay R
- Banerjee S
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- Broaddus VC
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- Brodsky JL
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- Bronson PG
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- Buckingham EM
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- Zorov DB
- Zorzano A
- Zou W
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- Zwerschke W
- Álvarez ÉMC
- Ávalos Y
- Önal G
- Üstün S
- Čolić M
- Đokić J
- Žerovnik E
- Publication venue
- 'Informa UK Limited'
- Publication date
- 01/01/2021
- Field of study
In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field
Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches
- Author
- Aase Handberg
- Abhijna R Ballal
- Abhimanyu Thakur
- Abuelreich Sarah
- Achille Anselmo
- Adam L Maddox
- Adityas Purnianto
- Adrian E Morelli
- Adrian L Harris
- Adriana F Paes Leme
- Adriana T Larregina
- Afrodité Németh
- Afsareen Bano
- Agnieszka Razim
- Agustin Enciso-Martinez
- Ahmed Ge Ibrahim
- Ahmed Somaida
- Aija Linē
- Ainhoa Alberro
- Ajay P Singh
- Akbar L Marzan
- Akhil Srivastava
- Akira Yokoi
- Al Charest
- Alain R Brisson
- Alan J Zimmerman
- Albano Cáceres-Verschae
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- Tomofumi Yamamoto
- Tong Wang
- Torrecilhas Ana C.
- Tosar Juan P.
- Trude Aspelin
- Tsuneya Ikezu
- Ursula S Sandau
- Useckaite Zivile
- Uta Erdbrügger
- Valentina R Minciacchi
- Valentina Zappulli
- Valeria Barreca
- Valle Francesco
- van der Pol Edwin
- van Herwijnen Martijn J. C.
- Varga Zoltan
- Vasiliki Mahairaki
- Vasiliy S Chernyshev
- Vatsal Deliwala
- Veer Bala Gupta
- Vendula Pospichalova
- Vengala Rao Yenuganti
- Venkatesh Kumar Chetty
- Vera A Tang
- Vera Mugoni
- Vera Tscherrig
- Verity J Ford
- Verline Justilien
- Veronica Huber
- Veronika Kralj-Iglic
- Veronika Stoka
- Victor Bodart-Santos
- Vijaya Sunkara
- Vikramsingh Gujar
- Viktoria Szeifert
- Viktoria Weber
- Vincent Hyenne
- Vincent Yeung
- Vincenza Dolo
- Violaine Moreau
- Vito G D'Agostino
- Vivek Kumar Gupta
- Vladimir Bokun
- Vroniqa Ku'ulei-Lyn Faustino
- Vytautas Žėkas
- Wai-Leng Lee
- Wanessa F Altei
- Wauben Marca H. M.
- Wehman Ann M.
- Wei Duan
- Wei Guo
- Wei Seong Toh
- Welsh Joshua A.
- Wen-Wei Chang
- Wilfrid Boireau
- Wilfried W Bazié
- Willem Stoorvogel
- Williams Sarah
- Witwer Kenneth W.
- Wolfgang Holnthoner
- Wyatt N Vreeland
- Xabier Osteikoetxea
- Xandra O Breakefield
- Xavier Loyer
- Xiaomei Yan
- Xiaoqin Wang
- Xinhua Lei
- Xinlei Li
- Xiuming Liang
- Yagmur Yildizhan
- Yang You
- Yasuo Inoshima
- Yi Wen
- Yinghong Zhou
- Yingtang Gao
- Yiyao Huang
- Yoel Sadovsky
- Yohan Kim
- Yong Song Gho
- Yongkang Yang
- Yonis Bare
- Yoon-Kyoung Cho
- Yoshinobu Takakura
- Young-Gyun Park
- Yu Fujita
- Yu Xiao
- Yuki Yamamoto
- Yumi Kumagai
- Yunjie Wu
- Yunxi Chen
- Yusuke Yamamoto
- Yusuke Yoshioka
- Yvan Courageux
- Yvonne Couch
- Zach Troyer
- Zendrini Andrea
- Zheng Lei
- Zheng Zhao
- Zijlstra Andries
- Zimmerman Alan J.
- Zivile Useckaite
- Zoltan Varga
- Zoltán Varga
- Zoltán Wiener
- Zucai Suo
- Publication venue
- Publication date
- 01/01/2024
- Field of study
Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its 'Minimal Information for Studies of Extracellular Vesicles', which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly
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