Primera cita de Egeria densa Planchon (Hydrocharitaceae) en la cuenca hidrográfica del Guadalquivir.

First record for Egeria densa Planchon (Hydrocharitaceae) in Guadalquivir River Basin.Palabras Clave: Hidrófito, plantas alóctonas, Egeria densa, Cuenca Hidrográfica del Guadalquivir.Kew Words: Hydrophyte, alien plants, Egeria densa, Guadalquivir River Basin

Faster monitoring of the invasive alien species (IAS) Dreissena polymorpha in river basins through isothermal amplification

Zebra mussel (Dreissena polymorpha) is considered as one of the 100 most harmful IAS in the world. Traditional detection methods have limitations, and PCR based environmental DNA detection has provided interesting results for early warning. However, in the last years, the development of isothermal amplification methods has received increasing attention. Among them, loop-mediated isothermal amplification (LAMP) has several advantages, including its higher tolerance to the presence of inhibitors and the possibility of naked-eye detection, which enables and simplifies its potential use in decentralized settings. In the current study, a real-time LAMP (qLAMP) method for the detection of Dreissena polymorpha was developed and tested with samples from the Guadalquivir River basin, together with two real-time PCR (qPCR) methods using different detection chemistries, targeting a specific region of the mitochondrial gene cytochrome C oxidase subunit I. All three developed approaches were evaluated regarding specificity, sensitivity and time required for detection. Regarding sensitivity, both qPCR approaches were more sensitive than qLAMP by one order of magnitude, however the qLAMP method proved to be as specific and much faster being performed in just 9 min versus 23 and 29 min for the qPCR methods based on hydrolysis probe and intercalating dye respectivelyThis work was supported by a partnership agreement project between the Confederación Hidrográfica del Guadalquivir and INL for the development of a system for early detection of zebra mussels through analysis of environmental DNA, and by project Nanotechnology Based Functional Solutions (NORTE-01-0145-FEDER-000019), supported by Norte Portugal Regional Operational Programme (NORTE2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund. S. A. acknowledges the Portuguese funding institution FCT – Fundação para Ciência e Tecnologia for Ph.D. scholarship SFRH/BD/140396/2018S

Tumor Associated Stromal Cells Play a Critical Role on the Outcome of the Oncolytic Efficacy of Conditionally Replicative Adenoviruses

The clinical efficacy of conditionally replicative oncolytic adenoviruses (CRAd) is still limited by the inefficient infection of the tumor mass. Since tumor growth is essentially the result of a continuous cross-talk between malignant and tumor-associated stromal cells, targeting both cell compartments may profoundly influence viral efficacy. Therefore, we developed SPARC promoter-based CRAds since the SPARC gene is expressed both in malignant cells and in tumor-associated stromal cells. These CRAds, expressing or not the Herpes Simplex thymidine kinase gene (Ad-F512 and Ad(I)-F512-TK, respectively) exerted a lytic effect on a panel of human melanoma cells expressing SPARC; but they were completely attenuated in normal cells of different origins, including fresh melanocytes, regardless of whether cells expressed or not SPARC. Interestingly, both CRAds displayed cytotoxic activity on SPARC positive-transformed human microendothelial HMEC-1 cells and WI-38 fetal fibroblasts. Both CRAds were therapeutically effective on SPARC positive-human melanoma tumors growing in nude mice but exhibited restricted efficacy in the presence of co-administered HMEC-1 or WI-38 cells. Conversely, co-administration of HMEC-1 cells enhanced the oncolytic efficacy of Ad(I)-F512-TK on SPARC-negative MIA PaCa-2 pancreatic cancer cells in vivo. Moreover, conditioned media produced by stromal cells pre-infected with the CRAds enhanced the in vitro viral oncolytic activity on pancreatic cancer cells, but not on melanoma cells. The whole data indicate that stromal cells might play an important role on the outcome of the oncolytic efficacy of conditionally replicative adenoviruses

[[alternative]]Examining the effects of using game-based APP for high school athletic-class students in learning physics

碩士[[abstract]]本研究旨在探討高中體育班學生在使用數位遊戲式APP學習基礎物理－力學單元的學習成效及其學習態度與動機。本研究以徐新逸及周雲虎（2012）研發之兩種數位遊戲式學習力學APP－牛頓兔與超級平衡為教材，將數位遊戲式APP應用於課堂學習，使用兩種遊戲軟體作為學習工具，探討其是否可提高學習態度與動機，增進其學習成效。 本研究採「準實驗設計」，採隨機分派分組，研究對象為89位新北市兩所高中的高一、高二體育班學生，分為實驗組與控制組進行實驗，實施「物理學習成效試卷」、「學習態度問卷（包含動機）」之前後測，並進行描述性統計、獨立樣本t檢定、課程觀察及訪談紀錄來檢視實驗結果。 研究結果顯示，數位遊戲式APP在高中體育班學習物理比對傳統教學法時學生的學習成效有顯著差異（p<.05），而學生的學習態度無顯著差異，但學習動機有顯著差異（p<.05）。結果建議，體育班高中生在經過數位遊戲式APP實驗後，其物理成績有顯著提升，且對其學習物理時也有助於改善其學習動機。[[abstract]]The purpose of this research was to investigate learning achievement, learning attitude, and learning motivation on athletic-class students in high school who thus received the digital game-based learning application to learn basic physics. This study utilized Newton Rabbit and Super Balance (Game-based APP) developed by Shyu & Chou (2012) as learning tools to explore whether it could improve learning attitude, motivation and enhance learning achievement in physics. This study adopted quasi-experimental research method. There were 89 high-school students from New Taipei City who were divided into an experimental group and a control group. The results showed that high school students learning physics with digital game-based learning application had significant differences on learning achievement and learning motivation (p <.05) but not learning attitude. Results implied that high school students in athletic-class after the experiment significantly improved on learning achievement and motivation in physics as well.[[tableofcontents]]目次 第一章 緒論 第一節 研究背景與動機 1 第二節 研究目的與待答問題 6 第三節 重要名詞定義 7 第四節 研究範圍與限制 10 第五節 預期貢獻 11 第二章 文獻探討 第一節 數位悅趣式學習 12 第二節 高級中學物理課程簡介與現況 29 第三節 體育班現況調查 34 第三章 研究方法 第一節 研究架構 37 第二節 研究流程與設計 38 第三節 研究對象與場所 40 第四節 研究工具 41 第五節 資料處理與分析 47 第四章 研究結果與討論 第一節 兩組學生物理學習成效之比較 49 第二節 兩組學生物理學習態度與動機之比較 53 第五章 結論與建議 第一節 研究結論 56 第二節 研究建議 60 參考文獻: 62 中文部分 62 英文部分 68 附錄 壹、物理學習態度與動機問卷（前後測） 70 貳、物理學習成效試卷（前測） 73 參、物理學習成效試卷（後測） 74 肆、物理遊戲APP施測家長同意書 76 伍、訪談大綱紀錄表編碼 77 陸、控制組觀察紀錄表1 80 柒、控制組觀察紀錄表2 81 捌、實驗組觀察紀錄表3 82 玖、實驗組觀察紀錄表4 83 表次 表2-1-1 悅趣式學習重要性整理 16 表2-1-2 悅趣式學習學者理論特性整理 21 表2-1-3 數位悅趣式學習現況調查 23 表2-1-4 電玩遊戲類型介紹 25 表2-2-1 教育部公布體育班課程綱要－以基礎物理力學篇 32 表2-3-1 體育班現況整理 34 表3-1-1 研究人數統計表 38 表3-1-2 兩組施測流程表 39 表3-3-1 實驗設計內容 40 表3-4-1學習成效之前測雙向細目表 45 表3-4-2 學習成效之後測雙向細目表 45 表4-1-1 前後測學習成效平均 49 表4-1-2 學習成效前後測成績獨立樣本t檢定摘要表 50 表4-1-3 訪談題目紀錄表 51 表4-2-1 實驗組與控制組學習態度與動機前後測對照表 53 表4-2-2 前測變異數相等的Levene檢定表 54 表4-2-3 後測學習態度與動機獨立樣本t檢定摘要表 55 圖次 圖2-1-1 數位遊戲學習模式 22 圖3-1-1 研究架構 37 圖3-4-1 牛頓兔遊戲畫面－關卡一 41 圖3-4-2 牛頓兔遊戲畫面－關卡二 42 圖3-4-3 牛頓兔遊戲畫面－關卡三 42 圖3-4-6 靜力平衡公式圖解 43 圖3-4-7 超級平衡關卡畫面－二力平衡 43 圖3-4-8 超級平衡關卡畫面－力矩平衡 44[[note]]學號: 601730111, 學年度: 10

Engendrando desenvolvimento e etnicidade nas terras baixas do Pacífico colombiano Ser y tener: engendering development and ethnicity in the Pacific lowlands of Colômbia

Neste ensaio, exploro como as organizações e redes de mulheres afro-colombianas moldam e são moldadas por iniciativas do Estado para desenvolver e modernizar a região do Pacífico. Argumento que, agindo assim, elas mobilizam e vão além da retórica desenvolvimentista do Estado e do discurso de uma etnia negra 'gendrada' e da tradição das organizações políticas negras da região.<br>In this paper I explore how Afro-Colombian women's organizations and networks shape and are shaped by state initiatives to develop and modernize the Pacific region. I argue that in doing so these mobilize and go beyond the developmentalist rhetoric of the state and the discourse of gendered black ethnicity and tradition of black political organizations in the region

Erratum to: Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition) (Autophagy, 12, 1, 1-222, 10.1080/15548627.2015.1100356

non present