Substrate specificity and regioselectivity of fungal AA9 lytic polysaccharide monooxygenases secreted by Podospora anserina

International audienceBackground: The understanding of enzymatic polysaccharide degradation has progressed intensely in the past few years with the identification of a new class of fungal-secreted enzymes, the lytic polysaccharide monooxygenases (LPMOs) that enhance cellulose conversion. In the fungal kingdom, saprotrophic fungi display a high number of genes encoding LPMOs from family AA9 but the functional relevance of this redundancy is not fully understood. Results: In this study, we investigated a set of AA9 LPMOs identified in the secretomes of the coprophilous ascomycete Podospora anserina, a biomass degrader of recalcitrant substrates. Their activity was assayed on cellulose in synergy with the cellobiose dehydrogenase from the same organism. We showed that the total release of oxidized oligosaccharides from cellulose was higher for PaLPMO9A, PaLPMO9E, and PaLPMO9H that harbored a carbohydrate-binding module from the family CBM1. Investigation of their regioselective mode of action revealed that PaLPMO9A and PaLPMO9H oxidatively cleaved at both C1 and C4 positions while PaLPMO9E released only C1-oxidized products. Rapid cleavage of cellulose was observed using PaLPMO9H that was the most versatile in terms of substrate specificity as it also displayed activity on cello-oligosaccharides and beta-(1,4)-linked hemicellulose polysaccharides (e.g., xyloglucan, glucomannan). Conclusions: This study provides insights into the mode of cleavage and substrate specificities of fungal AA9 LPMOs that will facilitate their application for the development of future biorefineries

GSSP proposal for the upper Aptian substage in the Apt Basin (Vaucluse, SE France): Synthesis of the stratigraphic data

La synthèse des données stratigraphiques recueillies depuis plus d'une décennie dans la région stratotypique a permis d'établir une coupe composite des "marnes aptiennes" s'étageant de la Sous-Zone d'ammonites à Grandis jusqu'à la base de la Zone à Melchioris, soit depuis la Zone de foraminifères à Schackoina (Leupoldina) gr. cabri jusqu'à la Zone à Globigerinelloides algerianus. Cette coupe composite intègre les coupes partielles de Clavaillan, Pichouraz Sud et Est, La Tuilière, Les Gays et Gargas (Vaucluse, SE France). Notre révision a montré en particulier que le banc marno-calcaire, rapproché du "Niveau blanc" vocontien et jusqu'alors supposé unique et isochrone, qui divise la série marneuse affleurant dans les coupes de Clavaillan et de Pichouraz, correspond en fait à deux niveaux stratigraphiquement distincts. La présente étude confirme que la série visible des marnes aptiennes dans la région stratotypique, non compte tenu de la Formation supra-urgonienne "A1" de Leenhardt (1883), ne comprend que les termes sommitaux du Bédoulien et une partie inférieure (= Gargasien inférieur et moyen) de l'Aptien s.s. (i.e., sensu Orbigny, 1840). Étayée par une approche intégrée, la mise en parallèle de la série du Bassin d'Apt avec les termes contemporains du Bassin de Cassis-La Bédoule a également permis de détecter et de quantifier des variations importantes, non concomitantes, des rythmes d'accumulation sédimentaire entre les deux secteurs. Au final, la coupe de Clavaillan, située à 8 km à l'WSW de Gargas, constitue l'unique affleurement où la limite entre la Sous-Zone à Grandis et la Zone à Furcata est visible dans un contexte permettant de le proposer comme candidat à la fixation du GSSP du sous-étage Gargasien (ou de l'Aptien s.s. dans sa conception historique).The synthesis of the stratigraphic data collected over more than a decade in the stratotypic area has made it possible to establish a composite section of the "Aptian marls" from the Grandis ammonite Subzone to the base of the Melchioris Zone, i.e., from the Schackoina (Leupoldina) gr. cabri foraminiferal Zone to the Globigerinelloides algerianus Zone. This composite section includes the partial sections from Clavaillan, South and East Pichouraz, La Tuilière, Les Gays and Gargas (Vaucluse, SE France). In particular, our revision has shown that the marly calcareous bed, which divides the marly series of Clavaillan and Pichouraz, compared with the Vocontian "Niveau blanc" and thought to be unique and isochronous, corresponds in fact to two stratigraphically distinct levels. The present study confirms that the visible series of marls in the stratotypic area, not taking into account the "A1" supra-Urgonian formation of Leenhardt (1883), includes only the uppermost terms of the Bedoulian and the lower terms (= Lower and Middle Gargasian) of the Aptian s.s. (i.e., sensu Orbigny, 1840). Based on the methods of integrated stratigraphy, a comparison of the series of the Apt Basin with the contemporary terms of the Cassis-La Bédoule Basin has also made it possible to detect and quantify important variations in the rhythms of sedimentary accumulation between the two sectors. In the end, the Clavaillan section, located 8 km WSW from the Gargas section, is the only outcrop where the boundary between the Grandis Subzone and the Furcata Zone is visible in a context which supports a proposal that this exposure be a candidate for the GSSP of the Gargasian substage (or of the Aptian s.s. in the initial concept of the creator of the stage)

Lytic xylan oxidases from wood-decay fungi unlock biomass degradation

Wood biomass is the most abundant feedstock envisioned for the development of modern biorefineries. However, the cost-ef-fective conversion of this form of biomass into commodity products is limited by its resistance to enzymatic degradation. Here we describe a new family of fungal lytic polysaccharide monooxygenases (LPMOs) prevalent among white-rot and brown-rot basidiomycetes that is active on xylans—a recalcitrant polysaccharide abundant in wood biomass. Two AA14 LPMO members from the white-rot fungus Pycnoporus coccineus substantially increase the efficiency of wood saccharification through oxida-tive cleavage of highly refractory xylan-coated cellulose fibers. The discovery of this unique enzyme activity advances our knowledge on the degradation of woody biomass in nature and offers an innovative solution for improving enzyme cocktails for biorefinery applications

COVID-19 symptoms at hospital admission vary with age and sex: results from the ISARIC prospective multinational observational study

Background: The ISARIC prospective multinational observational study is the largest cohort of hospitalized patients with COVID-19. We present relationships of age, sex, and nationality to presenting symptoms. Methods: International, prospective observational study of 60 109 hospitalized symptomatic patients with laboratory-confirmed COVID-19 recruited from 43 countries between 30 January and 3 August 2020. Logistic regression was performed to evaluate relationships of age and sex to published COVID-19 case definitions and the most commonly reported symptoms. Results: ‘Typical’ symptoms of fever (69%), cough (68%) and shortness of breath (66%) were the most commonly reported. 92% of patients experienced at least one of these. Prevalence of typical symptoms was greatest in 30- to 60-year-olds (respectively 80, 79, 69%; at least one 95%). They were reported less frequently in children (≤ 18 years: 69, 48, 23; 85%), older adults (≥ 70 years: 61, 62, 65; 90%), and women (66, 66, 64; 90%; vs. men 71, 70, 67; 93%, each P < 0.001). The most common atypical presentations under 60 years of age were nausea and vomiting and abdominal pain, and over 60 years was confusion. Regression models showed significant differences in symptoms with sex, age and country. Interpretation: This international collaboration has allowed us to report reliable symptom data from the largest cohort of patients admitted to hospital with COVID-19. Adults over 60 and children admitted to hospital with COVID-19 are less likely to present with typical symptoms. Nausea and vomiting are common atypical presentations under 30 years. Confusion is a frequent atypical presentation of COVID-19 in adults over 60 years. Women are less likely to experience typical symptoms than men

Intérêt de la ventilation non invasive au long cours dans le traitement de l'insuffisance cardiaque chronique sévère

MONTPELLIER-BU Médecine UPM (341722108) / SudocPARIS-BIUM (751062103) / SudocMONTPELLIER-BU Médecine (341722104) / SudocSudocFranceF

Real-time atrial wall imaging during radiofrequency ablation in a porcine model

International audienceBACKGROUND Real-time monitoring of radiofrequency (RF) abla-tion remains challenging. OBJECTIVE We used intravascular ultrasound (IVUS) to describe atrial wall changes during RF ablation and to assess the extent of RF-induced lesions. METHODS In 9 piglets, RF and IVUS catheters were coupled and introduced into the right atrium. RF applications were performed along the intercaval line. Corresponding IVUS images were analyzed. Wall thickness was correlated with electrogram (EGM) changes (n ¼ 9) and histology (n ¼ 5). RESULTS There were 66 RF applications performed in 57 sites. IVUS provided real-time imaging of the atrial wall during RF application in all but 2 sites. IVUS demonstrated significant (420%) and immediate increase in atrial wall thickness in 71.4% of RF applications. It showed epicardial or intramyocardial effusion in 30% of cases, 2 steam pops, 1 intramural hematoma, and 1 thrombus. EGM amplitude decreased and thickness increased after RF application than at baseline (2.20 1.11 to 0.99 0.62 mV and 1.34 0.53 to 1.93 0.80 mm, respectively; P o .001 for each). However, EGM and thickness changes were poorly correlated (r ¼ 0.43; P o .05). Histologically and echographically measured thicknesses were correlated (r ¼ 0.71; P ¼ .004), but echographic thickness change was not related to histological lesion transmurality. CONCLUSION An IVUS probe coupled to an RF catheter can provide relevant real-time imaging of the atrial wall during ablation. Although thickness change does not appear as a good predictor of the transmural extent, direct visualization and monitoring of RF application may provide new information to guide and secure RF ablation. KEYWORDS Radiofrequency ablation; RF ablation; Transmural extent of RF lesions; Radiofrequency ablation in a porcine model; IVUS imaging of atrial wall; RF lesion extent; Transmural lesion extent; Atrial fibrillation ABBREVIATIONS EGM ¼ electrogram; IVUS ¼ intravascular ultrasound; RA ¼ right atrium; RF ¼ radiofrequency

Multi-scale development of a stratiform chromite ore body at the base of the dunitic mantle-crust transition zone (Maqsad diapir, Oman ophiolite): The role of repeated melt and fluid influxes

International audienc