Explaining trends in alcohol-related harms in Scotland 1991–2011 (II): policy, social norms, the alcohol market, clinical changes and a synthesis

Objective: To provide a basis for evaluating post-2007 alcohol policy in Scotland, this paper tests the extent to which pre-2007 policy, the alcohol market, culture or clinical changes might explain differences in the magnitude and trends in alcohol-related mortality outcomes in Scotland compared to England & Wales (E&W). Study design: Rapid literature reviews, descriptive analysis of routine data and narrative synthesis. Methods: We assessed the impact of pre-2007 Scottish policy and policy in the comparison areas in relation to the literature on effective alcohol policy. Rapid literature reviews were conducted to assess cultural changes and the potential role of substitution effects between alcohol and illicit drugs. The availability of alcohol was assessed by examining the trends in the number of alcohol outlets over time. The impact of clinical changes was assessed in consultation with key informants. The impact of all the identified factors were then summarised and synthesised narratively. Results: The companion paper showed that part of the rise and fall in alcohol-related mortality in Scotland, and part of the differing trend to E&W, were predicted by a model linking income trends and alcohol-related mortality. Lagged effects from historical deindustrialisation and socio-economic changes exposures also remain plausible from the available data. This paper shows that policy differences or changes prior to 2007 are unlikely to have been important in explaining the trends. There is some evidence that aspects of alcohol culture in Scotland may be different (more concentrated and home drinking) but it seems unlikely that this has been an important driver of the trends or the differences with E&W other than through interaction with changing incomes and lagged socio-economic effects. Substitution effects with illicit drugs and clinical changes are unlikely to have substantially changed alcohol-related harms: however, the increase in alcohol availability across the UK is likely to partly explain the rise in alcohol-related mortality during the 1990s. Conclusions: Future policy should ensure that alcohol affordability and availability, as well as socio-economic inequality, are reduced, in order to maintain downward trends in alcohol-related mortality in Scotland

The affinity purification and characterization of ATP synthase complexes from mitochondria.

The mitochondrial F₁-ATPase inhibitor protein, IF₁, inhibits the hydrolytic, but not the synthetic activity of the F-ATP synthase, and requires the hydrolysis of ATP to form the inhibited complex. In this complex, the α-helical inhibitory region of the bound IF₁ occupies a deep cleft in one of the three catalytic interfaces of the enzyme. Its N-terminal region penetrates into the central aqueous cavity of the enzyme and interacts with the γ-subunit in the enzyme's rotor. The intricacy of forming this complex and the binding mode of the inhibitor endow IF₁ with high specificity. This property has been exploited in the development of a highly selective affinity procedure for purifying the intact F-ATP synthase complex from mitochondria in a single chromatographic step by using inhibitor proteins with a C-terminal affinity tag. The inhibited complex was recovered with residues 1-60 of bovine IF₁ with a C-terminal green fluorescent protein followed by a His-tag, and the active enzyme with the same inhibitor with a C-terminal glutathione-S-transferase domain. The wide applicability of the procedure has been demonstrated by purifying the enzyme complex from bovine, ovine, porcine and yeast mitochondria. The subunit compositions of these complexes have been characterized. The catalytic properties of the bovine enzyme have been studied in detail. Its hydrolytic activity is sensitive to inhibition by oligomycin, and the enzyme is capable of synthesizing ATP in vesicles in which the proton-motive force is generated from light by bacteriorhodopsin. The coupled enzyme has been compared by limited trypsinolysis with uncoupled enzyme prepared by affinity chromatography. In the uncoupled enzyme, subunits of the enzyme's stator are degraded more rapidly than in the coupled enzyme, indicating that uncoupling involves significant structural changes in the stator region

Reducing dose for digital cranial radiography : The increased source to the image-receptor distance approach

This investigation proposes that an increased source to the image-receptor distance (SID) technique can be used to optimize occipital frontal and lateral cranial radiographs acquired with direct digital radiography. Although cranial radiography is not performed on a routine basis, it should nonetheless be optimized to keep the dose to the patient as low as reasonably achievable, particularly because it can form part of the facial bone and sinus series. Dose measurements were acquired at various SIDs, and image quality was assessed using visual grading analysis. Statistically significant reductions in the effective dose between 19.2% and 23.9% were obtained when the SID was increased from the standard 100 to 150 cm (P ≤.05), and visual grading analysis scores indicate that image quality remained diagnostically acceptable for both projections. This investigation concludes that increasing the SID effectively optimizes occipital frontal and lateral skull radiographs. Radiology departments must be advised of the benefits of this technique with the goal of introducing an updated reference SID of 150 cm into clinical practice.Peer reviewe

The gravitationally lensed, luminous infrared galaxy IRAS F10214+4724 observed with XMM-Newton

We report on a short XMM-Newton observation of the gravitationally-lensed, luminous infrared galaxy IRAS F10214+4724 at z=2.3. A faint X-ray source is detected at 4 sigma. The observed 0.5-2 keV (1.7-6.6 keV in the rest-frame) flux is 1.3e-15 erg/s/cm2 and the spectral slope in the rest-frame 1-10 keV band is Gamma~2. These results agree with those obtained from the Chandra X-ray Observatory, given the large uncertainties in both measurements. While possible evidence for excess emission above 5 keV is seen, we suspect this excess might be either spurious or not related to the infrared galaxy.Comment: 4 pages, 2 figures, New Astronomy in pres

The structure of F₁-ATPase from Saccharomyces cerevisiae inhibited by its regulatory protein IF₁.

The structure of F₁-ATPase from Saccharomyces cerevisiae inhibited by the yeast IF₁ has been determined at 2.5 Å resolution. The inhibitory region of IF₁ from residues 1 to 36 is entrapped between the C-terminal domains of the α(DP)- and β(DP)-subunits in one of the three catalytic interfaces of the enzyme. Although the structure of the inhibited complex is similar to that of the bovine-inhibited complex, there are significant differences between the structures of the inhibitors and their detailed interactions with F₁-ATPase. However, the most significant difference is in the nucleotide occupancy of the catalytic β(E)-subunits. The nucleotide binding site in β(E)-subunit in the yeast complex contains an ADP molecule without an accompanying magnesium ion, whereas it is unoccupied in the bovine complex. Thus, the structure provides further evidence of sequential product release, with the phosphate and the magnesium ion released before the ADP molecule.Support for this work was provided by the Medical Research Council, UK, including a PhD studentship (to G.C.R.) and a Career Training Fellowship (to J.V.B.), by the European Drug Initiative in Channels and Transporters (EDICT; to J.E.W.), and by a grant from NIH no. R01GM66223 to D.M.M

3-dimensional Cauchy-Riemann structures and 2nd order ordinary differential equations

The equivalence problem for second order ODEs given modulo point transformations is solved in full analogy with the equivalence problem of nondegenerate 3-dimensional CR structures. This approach enables an analog of the Feffereman metrics to be defined. The conformal class of these (split signature) metrics is well defined by each point equivalence class of second order ODEs. Its conformal curvature is interpreted in terms of the basic point invariants of the corresponding class of ODEs

The Case of AB Aurigae's Disk in Polarized Light: Is There Truly a Gap?

Using the NICMOS coronagraph, we have obtained high-contrast 2.0 micron imaging polarimetry and 1.1 micron imaging of the circumstellar disk around AB Aurigae on angular scales of 0.3-3 arcsec (40-550 AU). Unlike previous observations, these data resolve the disk in both total and polarized intensity, allowing accurate measurement of the spatial variation of polarization fraction across the disk. Using these observations we investigate the apparent "gap" in the disk reported by Oppenheimer et al. 2008. In polarized intensity, the NICMOS data closely reproduces the morphology seen by Oppenheimer et al., yet in total intensity we find no evidence for a gap in either our 1.1 or 2.0 micron images. We find instead that region has lower polarization fraction, without a significant decrease in total scattered light, consistent with expectations for back-scattered light on the far side of an inclined disk. Radiative transfer models demonstrate this explanation fits the observations. Geometrical scattering effects are entirely sufficient to explain the observed morphology without any need to invoke a gap or protoplanet at that location.Comment: Accepted to ApJ Letter

Fishery benefits from exploiting spawning aggregations not solely dependent on enhanced fish density

The vulnerability of spawning aggregations to exploitation varies among fisheries as a result of differences in the population-density changes associated with this behaviour. However, vulnerability to fishing is also influenced by technology, environmental factors, and fish and fisher behaviours. Focusing on a fishery for the rabbitfish Siganus sutor at Praslin Island, Seychelles, we examined how catch rate varied across spawning and non-spawning habitats in relation to in situ population-density changes and other factors known to influence catchability. Catch rates in spawning habitat were disproportionate to density changes, being only fourfold greater than catch rates in non-spawning habitat, despite the fact that spawning-aggregation formation involved nine- to thirteen-fold increases in population density. Catch rates in spawning habitat were also highly variable across the spawning season (0–23.4 fish trap-hour−1). Current strength was of similar importance to density as a catch-rate predictor, with the highest catch rates in spawning habitats confined to months with the strongest currents. Therefore, in addition to density-dependent catchability, other factors that influence catch rates must be examined to avoid overestimation of the vulnerability of populations to aggregation fishing. The dynamics of catchability at spawning sites can limit the ability of fishers to predict and maximise returns based on increases in fish density