Program for Dog Pedigree Management

Tato bakalářská práce se zabývá návrhem a implementací programu pro vytváření a správu psích rodokmenů v jazyce Java. Navržený program je přenositelný a je schopen provádět operace nad databází psů, umožňuje procházet rodokmeny vybraných psů a pro vybranou fenu vyhledat vhodného psa ke krytí. V závěru práce jsou zhodnoceny přínosy této aplikace a nastíněny její další možnosti rozšíření.This bachelor´s thesis deals with a project and  system implementation of a program for dog pedigree management in Java. The suggested program  is cross-platform and also able to realise operations with the database of dogs. It is enable to browse family trees of dogs and find out the best dog for mating a selected female dog. In the conclusion benefits of this application are evaluated. Other posibilities of its extension have also been mentioned.

Family House – Water – Supply

Import 23/07/2015V mé bakalářské práci jsem se zabýval vypracováním projektové dokumentace stavby rodinného domu, navrženou pro 4 člennou rodinu, návrhu vnitřního vodovodu a zpětného využití dešťové vody. Objekt se skládá z přízemí, obytného podkroví a sedlové střechy. Projekt je rozdělen do dvou částí: Stavební část a Technické zařízení budov (zdravotechnika). Hlavním cílem této práce bylo navrhnout dle příslušných norem, funkční systém vnitřního vodovodu s ohřevem solárními kolektory s bivalentním zdrojem a potrubní síť dešťových vod se zpětným využitím ke splachování a zalévání zahrady.This thesis deals with the elaboration of project documentation of building a family house, designed for a family of 4 members, design of internal water conduit system and re-use of rainwater. The building consists of ground floor, residential loft and gabled roof. The project is divided into two parts: Construction part and technical equipment (sanitary part). The aim of this thesis was to design, according to the relevant standards, functional system of internal water conduit is heating by solar collectors with bivalent energy source and rainwater piping network for re-use to a flushing and garden watering.229 - Katedra prostředí staveb a TZBvýborn

Pension - Forced Ventilation with Heat Recovery

Import 13/01/2017V mé diplomové práci jsem se zabýval vypracováním projektové dokumentace pro provádění stavby penzionu, s celkovým počtem 11 pokojů s vlastními koupelnami a zádveřím, návrhu nuceného větrání s rekuperací. Objekt se skládá z přízemí a nadzemního podlaží zakončeným plochou střechou. Objekt je rozdělen na dvě samostatné zóny. Projekt se dělí do dvou částí: Stavební část a Technické zařízení budov (vzduchotechnika). Hlavním cílem této práce bylo navrhnout dle příslušných norem, funkční systém nucené výměny vzduchu ve všech místnostech příslušných zón.In my thesis I dealt with the drafting of project documentation for construction of of the pension, with a total of 11 rooms with ensuite and vestibule draft ventilation system with recuperation. The building consists a ground floor and a second floor ending with a flat roof. The building is divided into two separate zones. The project is divided into two parts: Part Construction and Technical Building Services (air conditioning). The main objective of this study was to design according to relevant standards, a functional system of forced air exchange in all rooms of the respective zones.229 - Katedra prostředí staveb a TZBvelmi dobř

Spatio-temporal analysis of metabolite profiles during barley germination

Kölling J, Gorzolka K, Niehaus K, Nattkemper TW. Spatio-temporal analysis of metabolite profiles during barley germination. Presented at the German Conference on Bioinformatics (GCB), Bielefeld, Germany

Calcium‐dependent protein kinase 5 links calcium signaling with N‐hydroxy‐l‐pipecolic acid‐ and SARD1‐dependent immune memory in systemic acquired resistance

Systemic acquired resistance (SAR) prepares infected plants for faster and stronger defense activation upon subsequent attacks. SAR requires an information relay from primary infection to distal tissue and the initiation and maintenance of a self‐maintaining phytohormone salicylic acid (SA)‐defense loop. In spatial and temporal resolution, we show that calcium‐dependent protein kinase CPK5 contributes to immunity and SAR. In local basal resistance, CPK5 functions upstream of SA synthesis, perception, and signaling. In systemic tissue, CPK5 signaling leads to accumulation of SAR‐inducing metabolite N‐hydroxy‐L‐pipecolic acid (NHP) and SAR marker genes, including Systemic Acquired Resistance Deficient 1 (SARD1) Plants of increased CPK5, but not CPK6, signaling display an ‘enhanced SAR’ phenotype towards a secondary bacterial infection. In the sard1‐1 background, CPK5‐mediated basal resistance is still mounted, but NHP concentration is reduced and enhanced SAR is lost. The biochemical analysis estimated CPK5 half maximal kinase activity for calcium, K50 [Ca2+], to be c. 100 nM, close to the cytoplasmic resting level. This low threshold uniquely qualifies CPK5 to decode subtle changes in calcium, a prerequisite to signal relay and onset and maintenance of priming at later time points in distal tissue. Our data explain why CPK5 functions as a hub in basal and systemic plant immunity

Spatio-Temporal Metabolite Profiling of the Barley Germination Process by MALDI MS Imaging

Gorzolka K, Kölling J, Nattkemper TW, Niehaus K. Spatio-Temporal Metabolite Profiling of the Barley Germination Process by MALDI MS Imaging. PLOS ONE. 2016;11(3): e0150208.MALDI mass spectrometry imaging was performed to localize metabolites during the first seven days of the barley germination. Up to 100 mass signals were detected of which 85 signals were identified as 48 different metabolites with highly tissue-specific localizations. Oligosaccharides were observed in the endosperm and in parts of the developed embryo. Lipids in the endosperm co-localized in dependency on their fatty acid compositions with changes in the distributions of diacyl phosphatidylcholines during germination. 26 potentially antifungal hordatines were detected in the embryo with tissue-specific localizations of their glycosylated, hydroxylated, and O-methylated derivates. In order to reveal spatio-temporal patterns in local metabolite compositions, multiple MSI data sets from a time series were analyzed in one batch. This requires a new preprocessing strategy to achieve comparability between data sets as well as a new strategy for unsupervised clustering. The resulting spatial segmentation for each time point sample is visualized in an interactive cluster map and enables simultaneous interactive exploration of all time points. Using this new analysis approach and visualization tool germination-dependent developments of metabolite patterns with single MS position accuracy were discovered. This is the first study that presents metabolite profiling of a cereals’ germination process over time by MALDI MSI with the identification of a large number of peaks of agronomically and industrially important compounds such as oligosaccharides, lipids and antifungal agents. Their detailed localization as well as the MS cluster analyses for on-tissue metabolite profile mapping revealed important information for the understanding of the germination process, which is of high scientific interest

Endogenous but not sensory-driven activity controls migration, morphogenesis and survival of adult-born juxtaglomerular neurons in the mouse olfactory bulb.

The development and survival of adult-born neurons are believed to be driven by sensory signaling. Here, in vivo analyses of motility, morphology and Ca2+ signaling, as well as transcriptome analyses of adult-born juxtaglomerular cells with reduced endogenous excitability (via cell-specific overexpression of either Kv1.2 or Kir2.1 K+ channels), revealed a pronounced impairment of migration, morphogenesis, survival, and functional integration of these cells into the mouse olfactory bulb, accompanied by a reduction in cytosolic Ca2+ fluctuations, phosphorylation of CREB and pCREB-mediated gene expression. Moreover, K+ channel overexpression strongly downregulated genes involved in neuronal migration, differentiation, and morphogenesis and upregulated apoptosis-related genes, thus locking adult-born cells in an immature and vulnerable state. Surprisingly, cells deprived of sensory-driven activity developed normally. Together, the data reveal signaling pathways connecting the endogenous intermittent neuronal activity/Ca2+ fluctuations as well as enhanced Kv1.2/Kir2.1 K+ channel function to migration, maturation, and survival of adult-born neurons

Novel agmatine derivatives in Maerua edulis with bioactivity against Callosobruchus maculatus, a cosmopolitan storage insect pest

Food security in developing countries is threatened by crop pests and ectoparasites in livestock. Strategies for their management still rely on synthetic pesticides which are not always effective and the active ingredients persist in the environment with negative consequences for beneficial arthropods, farmers and consumers, hence necessitating research on sustainable alternatives. Botanical insecticides are increasingly relevant, typically having lower impacts on users, consumers and the environment. One example is the southern African shrub the Blue bush-berry, Maerua edulis. Recent work reported effective pest control using this plant species against cattle ticks, storage beetles and vegetable pests. However, little is known about the chemistry underlying activity and this is essential to optimize its use. Here, we identified two novel plant chemical tructures, the E and Z isomers of cinnamoyl-4-aminobutylguanidine along with the E and Z isomers of 4-hydroxycinnamoyl-4-aminobutylguanidine in the leaves of M. edulis. We isolated these compounds from the leaves and elucidated their chemical structures using various spectroscopic techniques including High Resolution Mass Spectrometry and Nuclear Magnetic Resonance Spectroscopy. We also identified a further 11 closely related structures of which 6 are tentatively reported here for the first time. Stachydrine and 3-hydroxystachydrine were also identified in the leaf extract, and occurred at very high concentrations; up to 2% w/w of dry leaves. We tested these two compounds, along with the 4 main cinnamoylamides and the crude M. edulis leaf extract against the cowpea bruchid Callosobruchus maculatus at concentrations equivalent to those present in extracts used by smallholder farmers. Mortality of insects exposed to crude plant extracts after 72 h was significantly higher than the untreated control although still lower than for insects exposed to rotenone, the positive control. The two new compounds and stachydrine showed similar activity to the crude extracts suggesting that these compounds explained the activity of the extract. After 6 days, the mortality of insects exposed to crude extracts and isolated compounds was similar to that recorded with the positive control. The stachydrine fraction and the E and Z isomers of cinnamoyl-4-aminobutylguanidine also inhibited oviposition activity in fecund female beetles. Our data show that methanol extracts of M. edulis were toxic to C. maculatus and inhibited oviposition even at 0.1% w/v so these foliar chemicals may explain the activity of the plant material. We also synthesized the amides which facilitated structural elucidation, produced adequate quantities for testing and demonstrated the potential for commercial synthesis

Matrix assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) for direct visualization of plant metabolites in situ

Direct visualization of plant tissues by matrix assisted laser desorption ionization-mass spectrometry imaging (MALDI-MSI) has revealed key insights into the localization of metabolites in situ. Recent efforts have determined the spatial distribution of primary and secondary metabolites in plant tissues and cells. Strategies have been applied in many areas of metabolism including isotope flux analyses, plant interactions, and transcriptional regulation of metabolite accumulation. Technological advances have pushed achievable spatial resolution to subcellular levels and increased instrument sensitivity by several orders of magnitude. It is anticipated that MALDI-MSI and other MSI approaches will bring a new level of understanding to metabolomics as scientists will be encouraged to consider spatial heterogeneity of metabolites in descriptions of metabolic pathway regulatio