Information-Seeking Question Intonation in Basque Spanish and Its Correlation with Degree of Contact and Language Attitudes

The present study analyzes the prosodic characteristics of the variety of Spanish in contact with Basque (in the Basque Country, Spain). We focus on information-seeking yes/no questions, which present different intonation contours in Spanish and Basque. In Castilian Spanish, these sentences end in a rising contour, whereas in Basque, they end in a falling or rising–falling circumflex contour. In our previous work, this topic was investigated among the urban populations of Bilbao and San Sebastian. The results were that 79% of information-seeking yes/no questions had final falling intonational configurations. All the speakers presented a substantial presence of final falls regardless of their linguistic profile, but there were differences among speakers in the degree of presence of such features. A correlation was observed between the dependent variable of ‘frequency of occurrence of final falls in absolute interrogatives’ and social factors, such as ‘degree of contact with Basque’ and ‘attitudes towards Basque and the Basque ethnolinguistic group’. The correlation was that the higher the degree of contact with Basque and the more positive the attitudes towards Basque and the Basque ethnolinguistic group, the greater the frequency of occurrence of final falling intonational contours in information-seeking absolute interrogatives. The interpretation of this correlation was that the adoption of the characteristic Basque prosody allows speakers to be recognized as members of the Basque community. In the present study, we focused on rural areas. Falling intonational contours at the end of information-seeking absolute interrogatives were even more common than in urban areas (93.4%), and no correlation was found with degree of contact with Basque and with attitudes towards Basque. Our interpretation is that in rural areas the presence of Basque in daily life is stronger, and that there is a consolidated variety of Spanish used by all speakers regardless of their attitudes. Thus, the adoption of intonating features of this language is not the only indicator belonging to the Basque ethnolinguistic group. Our study reveals the great relevance of subjective social factors, such as language attitudes, in the degree of convergence between two languages.This work has been funded by the Ministry of Science and Innovation (grant number FFI2016-80021-P), the Basque Government (grant number IT1396-19) and the University of the Basque Country (grant number GIU18/221)

Chemical biology tools to study Deubiquitinases and Ubl proteases

The reversible attachment of ubiquitin (Ub) and ubiquitin like modifiers (Ubls) to proteins are crucial post-translational modifications (PTMs)for many cellular processes. Not only do cells possess hundreds of ligases to mediate substrate specific modification with Ub and Ubls, but they also have a repertoire of more than 100 dedicated enzymes for the specific removal of ubiquitin (Deubiquitinases or DUBs) and Ubl modifications (Ubl-specific proteases or ULPs). Over the past two decades, there has been significant progress in our understanding of how DUBs and ULPs function ata molecular level and many novel DUBs and ULPs, including several new DUB classes, have been identified. Here, the development of chemical tools that can bind and trap active DUBs has played a key role. Since the introduction of the first activity-based probe for DUBs in 1986, several innovations have led to the development of more sophisticated tools to study DUBs and ULPs. In this review we discuss how chemical biology has led to the development of activity-based probes and substrates that have been invaluable to the study of DUBs and ULPs. We summarise our currently available toolbox, highlight the main achievements and give an outlook of how these tools may be applied to gain a better understanding of the regulatory mechanisms of DUBs and ULPs

The “hidden side” of intergroup contact: The role of perceived social structure in motivating support for social change among the disadvantaged and the advantaged

Intergroup contact is a powerful prejudice-reduction strategy, but research has also revealed its undesirable effects among the disadvantaged. To unravel these counterintuitive effects, we examine the explanatory role of sociostructural factors (permeability of group boundaries as well as stability and legitimacy of inequality) in the link between intergroup contact and both perceived group discrimination against the disadvantaged and support for collective action. We relied on quasirepresentative or nationwide samples of immigrants (N = 1,539) and host nationals (N = 838), who participated in two survey-based studies. Among the disadvantaged, contact was associated with less awareness of group discrimination and collective action intentions via more perceptions of permeability and/or stability. Among the advantaged, intergroup contact was associated with greater awareness of group discrimination and collective action intentions (primarily) via perceived illegitimacy of existing status disparities. Our findings elucidate the importance of structural factors in understanding the implications of intergroup contact in addressing inequality and inequity, as well as designing contact-based interventions

Spatially resolved proteomic profiling identifies tumor cell CD44 as a biomarker associated with sensitivity to PD-1 axis blockade in advanced non-small- cell lung cancer

Most patients with advanced non-small-cell lung cancer (NSCLC) fail to derive significant benefit from programmed cell death protein-1 (PD-1) axis blockade, and new biomarkers of response are needed. In this study, we aimed to discover and validate spatially resolved protein markers associated with sensitivity to PD-1 axis inhibition in NSCLC

Differential expansion of circulating human MDSC subsets in patients with cancer, infection and inflammation

Background Myeloid-derived suppressor cells (MDSC) are a functional myeloid cell subset that includes myeloid cells with immune suppressive properties. The presence of MDSC has been reported in the peripheral blood of patients with several malignant and non-malignant diseases. So far, direct comparison of MDSC across different diseases and Centers is hindered by technical pitfalls and a lack of standardized methodology. To overcome this issue, we formed a network through the COST Action Mye-EUNITER (www.mye-euniter.eu) with the goal to standardize and facilitate the comparative analysis of human circulating MDSC in cancer, inflammation and infection. In this manuscript, we present the results of the multicenter study Mye-EUNITER MDSC Monitoring Initiative, that involved 13 laboratories and compared circulating MDSC subsets across multiple diseases, using a common protocol for the isolation, identification and characterization of these cells. Methods We developed, tested, executed and optimized a standard operating procedure for the isolation and immunophenotyping of MDSC using blood from healthy donors. We applied this procedure to the blood of almost 400 patients and controls with different solid tumors and non-malignant diseases. The latter included viral infections such as HIV and hepatitis B virus, but also psoriasis and cardiovascular disorders. Results We observed that the frequency of MDSC in healthy donors varied substantially between centers and was influenced by technical aspects such as the anticoagulant and separation method used. Expansion of polymorphonuclear (PMN)-MDSC exceeded the expansion of monocytic MDSC (M-MDSC) in five out of six solid tumors. PMN-MDSC expansion was more pronounced in cancer compared with infection and inflammation. Programmed death-ligand 1 was primarily expressed in M-MDSC and e-MDSC and was not upregulated as a consequence of disease. LOX-1 expression was confined to PMN-MDSC. Conclusions This study provides improved technical protocols and workflows for the multi-center analysis of circulating human MDSC subsets. Application of these workflows revealed a predominant expansion of PMN-MDSC in solid tumors that exceeds expansion in chronic infection and inflammation

La entonación de las interrogativas absolutas de búsqueda de información en español de Madrid

Romera and Elordieta (2019) and Elordieta and Romera (2020) reported that most information-seeking absolute interrogatives in the variety of Spanish spoken in the Basque Country have a rising-falling circumflex final contour. Basque has falling final contours in absolute interrogatives. Applying the same methodology, this paper shows that most information-seeking absolute interrogatives in Madrid Spanish end in a high rising configuration. The results of this research strengthen the hypothesis proposed by Romera and Elordieta (2019) and Elordieta and Romera (2020) that the variety of Spanish spoken in the Basque Country shows influence from Basque.Romera y Elordieta (2019) y Elordieta y Romera (2020) informaron que la mayoría de los enunciados interrogativos absolutos neutros o de búsqueda de información en la variedad de español del País Vasco tienen un contorno final circunflejo ascendente-descendente. El euskera tiene contornos finales descendentes en enunciados interrogativos absolutos. Este artículo muestra que la mayoría de las preguntas absolutas de búsqueda de información en el español de Madrid terminan en una configuración tonal de subida. Los resultados refuerzan la hipótesis de Romera y Elordieta (2019) y Elordieta y Romera (2020) de que la variedad del español hablado en el País Vasco muestra influencia del euskera

The intonation of information-seeking absolute interrogatives in Madrid Spanish

Romera and Elordieta (2019) and Elordieta and Romera (2020) reported that most information-seeking absolute interrogatives in the variety of Spanish spoken in the Basque Country have a rising-falling circumflex final contour. Basque has falling final contours in absolute interrogatives. Applying the same methodology, this paper shows that most information-seeking absolute interrogatives in Madrid Spanish end in a high rising configuration. The results of this research strengthen the hypothesis proposed by Romera and Elordieta (2019) and Elordieta and Romera (2020) that the variety of Spanish spoken in the Basque Country shows influence from Basque