Interaction of a fluorescent analog of N -deacetyl-N -methyl-colchicine (colcemid) with liver alcohol dehydrogenase

The evidence for specific binding of N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-colcemid (NBD-colcemid), a fluorescent analog of colcemid (N-deacetyl-N-methyl-colchicine), to liver alcohol dehydrogenase is presented. Alcohol dehydrogenase bound NBD-colcemid in a time-dependent manner, enhanced the fluorescence intensity, and caused a large blue shift of the emission maximum of the free drug. The specificity of binding was determined for both the colchicine nucleus and the NBD moiety. The binding was not affected by the presence of alcohol or NAD in the reaction mixture. Preincubation of horse liver alcohol dehydrogenase with colcemid inhibited the binding to a considerable extent. NBD-colcemid inhibited the enzymic activity of alcohol dehydrogenase in a mixed-type noncompetitive mode with a Ki value of 32 μM, whereas colcemid showed noncompetitive inhibition with a Ki of 100 μM. The association rate constant of NBD-colcemid binding with liver alcohol dehydrogenase was 587 M-1 s-1 at 25 °C. The stoichiometry and dissociation constant of the binding reaction were 0.62/dimer and 12 μM, respectively. Donor quenching experiments showed that both tryptophans of alcohol dehydrogenase transferred energy to the bound NBD-colcemid. Thus, this study reports the binding of a colchicine analog to a protein other than tubulin with high affinity. It is concluded that NBD-colcemid binding to dehydrogenases is a general phenomenon, but the common structural element(s) that is responsible for the binding activity, and which exists among tubulin and dehydrogenases, has yet to be determined

Diversity and Abundance of Ammonia-Oxidizing Bacteria and Archaea in a Freshwater Recirculating Aquaculture System

The role of ammonia-oxidizing bacteria and archaea was evaluated using amoA gene in a freshwater recirculating system. Broken earthen pot pieces (BEP) were used as filter bed material. Five archaeal and four bacterial operational taxonomic units were retrieved from amoA genes. Shannon-Weiner and Simpson indices were higher in archaeal amoAsequence compared with the bacteria. Subtype diversity ratio and subtype diversity variance were 0.522 and 0.008, respectively, for archaea and 0.403 and 0.015, respectively, for bacteria. In archaea, 50% amoA sequences showed 99%–100% similarity with the known sequences of ammonia monooxygenase subunit A of uncultured archaeon clones and thaumarchaeote. In bacteria, 84% sequences showed 99% similarity with amoAsequences of different uncultured bacterial clone and Nitrosomonadaceae. Absolute quantification showed that the abundance of archaea was 12-fold higher compared with bacteria. In this recirculating system, ammonia-oxidizing archaea and bacteria played a major role; BEP supported the growth of these ammonia-oxidizing microorganisms

Mass Production of Lemna minor and Its Amino Acid and Fatty Acid Profiles

The surface floating duckweed Lemna minor (Lemnaceae) is a potential ingredient to replace the application of fish-meal in the aqua-feed. The culture technique of the duckweed was standardized in outdoor tanks and then applied in the pond. Three consecutive experiments were conducted in tanks (1.2 × 0.35 × 0.3 m). In experiment 1, four different manures were used. In manure 1 (organic manure, OM) and manure 3 (2x OM), cattle manure, poultry droppings, and mustard oil cake (1:1:1) were used; in manure 2 (inorganic fertilizer, IF), urea, potash, triple superphosphate were used; manure 4 (2x OM+IF) was a combination of manure 2 and manure 3. In experiment 2, manure 1 (OM) and manure 2 (IF) were used, and manure 3 (OM+IF) was a combination of both manures. In experiment 3, OM and IF were selected. In pond (20 × 10 × 0.5 m), OM was applied. Fresh duckweed was seeded after 5 days of manure application. In experiments 1 and 3, total production was significantly (P 0.05) difference in production between OM and IF. In pond, relative growth rate (RGR) of duckweed ranged from 0.422 to 0.073 g/g/day and total production was 702.5 Kg/ha/month (dry weight). Protein, lipid, and ash contents were higher in duckweed cultured in OM compared to IF. The duckweed was a rich source of essential (39.20%), non-essential (53.64%), and non-proteinogenic (7.13%) amino acids. Among essential amino acids, leucine, isoleucine, and valine constituted 48.67%. Glutamic acid was 25.87% of total non-essential amino acids. Citrulline, hydroxiproline, taurine, etc. were found in the duckweed. The fatty acid composition was dominated by PUFA, 60–63% of total fatty acids, largely α-linolenic acid (LNA, 18:3n-3) at around 41 to 47% and linoleic acid (LA, 18:2n-6) at 17–18%. The nutritional value of duckweeds and their production potential in the pond conditions were evaluated. Duckweed biomass may thus be used to replace commercial fish-meal that is currently used in aquaculture

Optical microvariability properties of BALQSOs

We present optical light curves of 19 radio quiet (RQ) broad absorption line (BAL) QSOs and study their rapid variability characteristics. Systematic CCD observations, aided by a careful data analysis procedure, have allowed us to clearly detect any such microvariability exceeding 0.01--0.02 mag. Our observations cover a total of 13 nights (~72 hours) with each quasar monitored for about 4 hours on a given night. Our sample size is a factor of three larger than the number of radio-quiet BALQSOs previously searched for microvariability. We introduce a scaled F-test statistic for evaluating the presence of optical microvariability and demonstrate why it is generally preferable to the statistics usually employed for this purpose. Considering only unambiguous detections of microvariability we find that ~11 per cent of radio-quiet BALQSOs (two out of 19 sources) show microvariability for an individual observation length of about 4 hr. This new duty cycle of 11 per cent is similar to the usual low microvariability fraction of normal RQQSOs with observation lengths similar to those of ours. This result provides support for models where radio-quiet BALQSO do not appear to be a special case of the RQQSOs in terms of their microvariability properties.Comment: 13 pages, 3 figures, 3 tables, accepted for publication in MNRAS main journa

Deuterium oxide stabilizes conformation of tubulin: a biophysical and biochemical study

The present study was aimed to elucidate the mechanism of stabilization of tubulin by deuterium oxide $(D_2O)$. Rate of decrease of tryptophan fluorescence during aging of tubulin at $4^oC$ and $37^oC$ was significantly lower in $D_2O$ than in $H_2O$. Circular dichroism spectra of tubulin after incubation at $4^oC$, suggested that complete stabilization of the secondary structure in $D_2O$ during the first 24 hours of incubation. The number of available cysteine measured by DTNB reaction was decreased to a lesser extent in $D_2O$ than in $H_2O$. During the increase in temperature of tubulin, the rate of decrease of fluorescence at 335 nm and change of CD value at 222 nm was lesser in $D_2O$. Differential Scanning calorimetric experiments showed that the $T_m$ values for tubulin unfolding in $D_2O$ were $58.6^oC$ and $62.17^oC$, and in $H_2O$ those values were $55.4^oC$ and $59.35^oC$

Growth and digestive enzyme activities of rohu labeo rohita fed diets containing macrophytes and almond oil-cake

The impact of plant-based diets on the digestive physiology of rohu Labeo rohita fingerlings (10.66 ± 0.53 g) was evaluated. A diet with all protein supplied by fishmeal was included as a control (F). Four test diets containing 300 g/kg protein were formulated using the following plant ingredients and fishmeal in a 1:1 blend: almond oil-cake Terminalia catappa (FTC), duckweed Lemna minor (FLM), water fern Salvania molesta (FSM) and combination of these three ingredients (FTCLMSM). The final body weight and specific growth rate were significantly higher in rohu fed diet FLM compared to the other treatments. Significantly lower feed conversion ratio in rohu fed diet FLM showed that diet was utilized efficiently in this feeding regime compared to the other diets. The composition of diets also influenced the digestive enzyme activities of the fish. Thus, amylase, trypsin and chymotrypsin activities were significantly higher in rohu fed diet FLM compared to the rohu fed the other diets. Protease activity was significantly higher in rohu fed diets FTC and F and lipase activity was significantly higher in rohu fed diet FTC compared to the rohu fed the other diets. The inclusion of raw duckweed in feed replaced 300 g/kg of dietary fishmeal without affecting growth

Simultaneous MITSuME gRI monitoring of S5 0716+714

We present results of our intra-night optical flux monitoring observations of S5 0716+714 done simultaneously in gRI filters. The observations were done using Multicolor Imaging Telescopes for Survey and Monstrous Explosions (MITSuME) instrument on the 50 cm telescope at the Okayama Astrophysical Observatory over 30 nights between 11 March 2008 and 8 May 2008. Of these 30 nights, 22 nights have continuous (without any break) observations with duration ranging from 1 to 6 hours and hence were considered for intra-night optical variability (INOV). In total we have 4888 datapoints which were simultaneous in gR and I filters. Of the 22 nights considered for INOV, the object showed flux variability on 19 nights with the amplitude of variability in the I-band ranging from ~4% to ~55%. The duty cycle for INOV was thus found to be 83%. No time lag between different bands was noticed on most of the nights, except for 3 nights where the variation in g was found to lead that of the I band by 0.3 to 1.5 hrs. On inter-night timescales, no lag was found between g and I bands. On inter-night timescales as well as intra-night timescales on most of the nights, the amplitude of variability was found to increase toward shorter wavelengths. The flux variations in the different bands were not achromatic, with the blazar tending to become bluer when brighter both on inter-night and intra-night timescales; and this might be attributed to the larger amplitude variation at shorter wavelengths. A clear periodic variation of 3.3 hrs was found on 1 April 2008 and a hint for another possible periodic variability of 4 hrs was found on 31 March 2008. During our 30 days of observations over a 2 month period the source has varied with an amplitude of variability as large as ~80%.Comment: 11 pages, 8 figures and 5 tables, Accepted in MNRA

A ~4.6 h quasi-periodic oscillation in the BL Lacertae PKS 2155-304?

We report a possible detection of an ~4.6-hour quasi-periodic oscillation (QPO) in the 0.3-10 keV emission of the high-energy peaked blazar PKS 2155-304 from a 64 ks observation by the XMM-Newton EPIC/pn detector. We identify a total modulation of ~5% in the light curve and confirm that nominal period by periodogram, structure function and wavelet analyses. The limited light curve duration allows the capture of only 3.8 cycles of this oscillation and thus precludes a very strong claim for this QPO, despite a nominally high (>3 sigma) statistical significance. We briefly discuss models capable of producing an X-ray QPO of such a period in a blazar.Comment: 4 pages, 6 figures, accepted for publication in A&A Letter

Optical Intraday Variability Studies of Ten Low Energy Peaked Blazars

We have carried out optical (R band) intraday variability (IDV) monitoring of a sample of ten bright low energy peaked blazars (LBLs). Forty photometric observations, of an average of ~ 4 hours each, were made between 2008 September and 2009 June using two telescopes in India. Measurements with good signal to noise ratios were typically obtained within 1-3 minutes, allowing the detection of weak, fast variations using N-star differential photometry. We employed both structure function and discrete correlation function analysis methods to estimate any dominant timescales of variability and found that in most of the cases any such timescales were longer than the duration of the observation. The calculated duty cycle of IDV in LBLs during our observing run is ~ 52%, which is low compared to many earlier studies; however, the relatively short periods for which each source was observed can probably explain this difference. We briefly discuss possible emission mechanisms for the observed variability.Comment: 20 Pages, 9 Figures, 4 Tables, accepted for publication in MNRA

From Moscow with love

One of the less researched aspects of postcolonial India’s “progressive” culture is its Soviet connection. Starting in the 1950s and consolidating in the 1960s, the Union of Soviet Socialist Republics invested in building up “committed” networks amongst writers, directors, actors, and other theater- and film-practitioners across India. Thus, an entire generation of cultural professionals was initiated into the anticolonial solidarity of emerging Afro-Asian nations that were seen, and portrayed, by the Soviets as being victims of “Western” imperialism. The aspirational figure of the New Soviet Man was celebrated through the rise of a new form of “transactional sociality” (Westlund 2003). This paper looks at selected cases of cultural diplomacy—through the lens of cultural history—between the USSR and India for two decades after India’s Independence, exploring the possibility of theorizing it from the perspective of an anticolonial cultural solidarity that allowed agency to Indian interlocutors