Cardinal temperatures and vernalisation requirements for a selection of vegetables for seed production

The cardinal temperatures and vernalisation requirements for a selection of vegetables for seed production were studied. Experiment one assessed the rate of germination (1/days) of selected vegetable species over a range of 5-40 °C, in order to calculate the cardinal (base, optimum and maximum) temperatures for germination. The cardinal temperatures for carrot and red beet were 0.1, 30.9 and 40.7 °C, and 4.2, 35.9 and 44.4 °C respectively. Experiment two examined the vernalisation response of imbibed cabbage, carrot and red beet seeds, plus perennial ryegrass as a comparison over duration treatments of 0-12 weeks at 4 °C. Red beet and ryegrass had positive vernalisation responses with anthesis occurring in plants from the 4-12 week durations in red beet, and all durations in ryegrass. The number of days to anthesis and the final number of main stem leaves did not differ significantly (P = 0.143 and P = 0.323 respectively) among vernalisation durations in red beet, but did differ significantly in ryegrass (P <0.001 and P = 0.004 respectively). The number of days to anthesis decreased by 3.6 days for every one week increase in vernalisation duration. The same pattern was observed for main stem leaf number in ryegrass, with a decrease of 0.1 leaves for every one week increase in vernalisation duration. This response is likely to be attributed to perennial ryegrass having a lower base temperature (1.1 °C) than the vernalisation treatment (4 °C), leading to continued development towards anthesis throughout the vernalisation period. Red beet had a base temperature of 4.6 °C, so may not have experienced development towards reproduction, or may have reached vernalisation saturation at ≤ 4 weeks, resulting in a minimum number of leaves produced for all durations. There is potential for the sowing date of red beet to be changed to spring for successfully vernalised seeds, which would reduce the expenses of weed, pest and disease control and give opportunity of other land uses over the winter period

Two types of shock in the hotspot of the giant quasar 4C74.26: a high-resolution comparison from Chandra, Gemini & MERLIN

New Chandra observations have resolved the structure of the X-ray luminous southern hotspot in the giant radio quasar 4C74.26 into two distinct features. The nearer one to the nucleus is an extremely luminous peak, extended some 5 kpc perpendicular to the orientation of the jet; 19 kpc projected further away from the central nucleus than this is a fainter X-ray arc having similar symmetry. This arc is co-spatial with near-IR and optical emission imaged with Gemini, and radio emission imaged with MERLIN. The angular separation of the double shock structure (itself ~19 kpc or 10 arcsec in size) from the active nucleus which fuels them of ~550 kpc is a reminder of the challenge of connecting "unidentified" hard X-ray or Fermi sources with their origins.Comment: In press at MNRA

Genome evolution in three species of cactophilic drosophila

We report genomes of two species of cactophilic Drosophila: Drosophila arizonae and D. navojoa. These two are the closest relatives of D. mojavensis, forming the D. mojavensis cluster. D. mojavensis and D. arizonae diverged from D. navojoa ∼5.8 Mya, while the split between D. arizonae and D. mojavensis is more recent, at 1.5 Mya. Together the three genomes provide opportunities to examine genomic changes associated with speciation and host shifts in this ecologically defined group of flies. The three species are also separated by fixed inversion differences in three of their six chromosomes. While the levels of nucleotide divergence in the colinear chromosomes are significantly lower than in the inverted chromosomes, consistent with a past role of the inversions in preventing gene flow, the patterns differ among the inverted chromosomes when the locations of nucleotides inside or outside of the inversions are considered. For Muller element E, there is greater divergence external to the inversion breakpoints. For Muller A, the divergence is slightly higher inside the inversions, while for Muller B, the breakpoints and hence the difference in substitutions in relation to the inversions could not be determined. The differences among the inverted chromosomes, especially once the breakpoints are clearly established, could aid in dating the origins of the inversions

A Transient Sub-Eddington Black Hole X-ray Binary Candidate in the Dust Lanes of Centaurus A

We report the discovery of a bright X-ray transient, CXOU J132527.6-430023, in the nearby early-type galaxy NGC 5128. The source was first detected over the course of five Chandra observations in 2007, reaching an unabsorbed outburst luminosity of 1-2*10^38 erg/s in the 0.5-7.0 keV band before returning to quiescence. Such luminosities are possible for both stellar-mass black hole and neutron star X-ray binary transients. Here, we attempt to characterize the nature of the compact object. No counterpart has been detected in the optical or radio sky, but the proximity of the source to the dust lanes allows for the possibility of an obscured companion. The brightness of the source after a >100 fold increase in X-ray flux makes it either the first confirmed transient non-ULX black hole system in outburst to be subject to detailed spectral modeling outside the Local Group, or a bright (>10^38 erg/s) transient neutron star X-ray binary, which are very rare. Such a large increase in flux would appear to lend weight to the view that this is a black hole transient. X-ray spectral fitting of an absorbed power law yielded unphysical photon indices, while the parameters of the best-fit absorbed disc blackbody model are typical of an accreting ~10 Msol black hole in the thermally dominant state.Comment: 8 pages, 6 figures, accepted for publication in Ap

The magnetized medium around the radio galaxy B2 0755+37: an interaction with the intra-group gas

We explore the magneto-ionic environment of the isolated radio galaxy B2 0755+37 using detailed imaging of the distributions of Faraday rotation and depolarization over the radio source from Very Large Array observations at 1385,1465 and 4860 MHz and new X-ray data from XMM-Newton. The Rotation Measure (RM) distribution is complex, with evidence for anisotropic fluctuations in two regions. The approaching lobe shows low and uniform RM in an unusual `stripe' along an extension of the jet axis and a linear gradient transverse to this axis over its Northern half. The leading edge of the receding lobe shows arc-like RM structures with sign reversals. Elsewhere, the RM structures are reasonably isotropic. The RM power spectra are well described by cut-off power laws with slopes ranging from 2.1 to 3.2 in different sub-regions. The corresponding magnetic-field autocorrelation lengths, where well-determined, range from 0.25 to 1.4 kpc. It is likely that the fluctuations are mostly produced by compressed gas and field around the leading edges of the lobes. We identify areas of high depolarization around the jets and inner lobes. These could be produced by dense gas immediately surrounding the radio emission containing a magnetic field which is tangled on small scales. We also identify four ways in which the well known depolarization (Faraday depth) asymmetry between jetted and counter-jetted lobes of extended radio sources can be modified by interactions with the surrounding medium.Comment: 16 pages, 13 figures, accepted for publication in MNRAS. Full resolution paper available at: ftp://ftp.ira.inaf.it/pub/outgoing/guidetti/ Subjects: Cosmology and Extragalactic Astrophysics (astro-ph.CO

Deep Chandra observations of Pictor A

This article has been accepted for publication in Monthly Notices of the Royal Astronomical Society. ©: 2015 The Authors. Published by Oxford University Press on behalf of the Royal Astronomical Society.We report on deep Chandra observations of the nearby broad-line radio galaxy Pictor A, which we combine with new Australia Telescope Compact Array (ATCA) observations. The new X-ray data have a factor 4 more exposure than observations previously presented and span a 15-year time baseline, allowing a detailed study of the spatial, temporal and spectral properties of the AGN, jet, hotspot and lobes. We present evidence for further time variation of the jet, though the flare that we reported in previous work remains the most significantly detected time-varying feature. We also confirm previous tentative evidence for a faint counterjet. Based on the radio through X-ray spectrum of the jet and its detailed spatial structure, and on the properties of the counterjet, we argue that inverse-Compton models can be conclusively rejected, and propose that the X-ray emission from the jet is synchrotron emission from particles accelerated in the boundary layer of a relativistic jet. For the first time, we find evidence that the bright western hotspot is also time-varying in X-rays, and we connect this to the small-scale structure in the hotspot seen in high-resolution radio observations. The new data allow us to confirm that the spectrum of the lobes is in good agreement with the predictions of an inverse-Compton model and we show that the data favour models in which the filaments seen in the radio images are predominantly the result of spatial variation of magnetic fields in the presence of a relatively uniform electron distribution.Peer reviewe

Ordered magnetic fields around radio galaxies: evidence for interaction with the environment

We present detailed imaging of Faraday rotation and depolarization for the radio galaxies 0206+35, 3C 270, 3C 353 and M 84, based on Very Large Array observations at multiple frequencies in the range 1365 to 8440 MHz. This work suggests a more complex picture of the magneto-ionic environments of radio galaxies than was apparent from earlier work. All of the sources show spectacular banded rotation measure (RM) structures with contours of constant RM perpendicular to the major axes of their radio lobes. We give a comprehensive description of the banded RM phenomenon and present an initial attempt to interpret it as a consequence of interactions between the sources and their surroundings. We show that the material responsible for the Faraday rotation is in front of the radio emission and that the bands are likely to be caused by magnetized plasma which has been compressed by the expanding radio lobes. A two-dimensional magnetic structure in which the field lines are a family of ellipses draped around the leading edge of the lobe can produce RM bands in the correct orientation for any source orientation. We also report the first detections of rims of high depolarization at the edges of the inner radio lobes of M 84 and 3C 270. These are spatially coincident with shells of enhanced X-ray surface brightness, in which both the field strength and the thermal gas density are likely to be increased by compression.Comment: 21 pages, 15 figures, accepted for publication in MNRAS. Full resolution paper available at http://www.ira.inaf.it/~guidetti/bands/ Subjects: Astrophysics (astro-ph

Highlights of the mini-symposium on extracellular vesicles in inter-organismal communication, held in Munich, Germany, August 2018

All living organisms secrete molecules for intercellular communication. Recent research has revealed that extracellular vesicles (EVs) play an important role in inter-organismal cell-to-cell communication by transporting diverse messenger molecules, including RNA, DNA, lipids and proteins. These discoveries have raised fundamental questions regarding EV biology. How are EVs biosynthesized and loaded with messenger/cargo molecules? How are EVs secreted into the extracellular matrix? What are the EV uptake mechanisms of recipient cells? As EVs are produced by all kind of organisms, from unicellular bacteria and protists, filamentous fungi and oomycetes, to complex multicellular life forms such as plants and animals, basic research in diverse model systems is urgently needed to shed light on the multifaceted biology of EVs and their role in inter-organismal communications. To help catalyse progress in this emerging field, a mini-symposium was held in Munich, Germany in August 2018. This report highlights recent progress and major questions being pursued across a very diverse group of model systems, all united by the question of how EVs contribute to inter-organismal communication

Task-Specific Recognition Signals Are Located on the Legs in a Social Insect

Task allocation ensures a high level of organization within social insect colonies. Workers reveal their task assignment through cuticular hydrocarbon (CHC) signals. The source and chemical composition of these signals are largely unknown. We ask whether task recognition signals are located on particular body parts of workers of Australian meat ants (Iridomyrmex purpureus). We analyzed the CHC profile on the antennae, legs, and abdomens of workers engaged in different tasks. Discriminant analysis showed that the leg profile is the best indicator of task identification. Behavioral assays confirmed this finding: workers typically reacted differently to non-nestmates engaged in different tasks, but not if the CHCs on the legs of their opponents were removed by a solvent. Lasso and Elastic-Net Regularized Generalized Linear Model (GLMNET) revealed which CHC components show the highest correlation in task and nestmate recognition, suggesting that social insects can simultaneously convey different CHC signals on different body parts, thereby allowing efficient signaling and signal perception.a University of Melbourne Postgraduate Scholarship and a Holsworth Wildlife Research Endowment (to QW)

Detecting microRNA binding and siRNA off-target effects from expression data.

Sylamer is a method for detecting microRNA target and small interfering RNA off-target signals in 3' untranslated regions from a ranked gene list, sorted from upregulated to downregulated, after a microRNA perturbation or RNA interference experiment. The output is a landscape plot that tracks occurrence biases using hypergeometric P-values for all words across the gene ranking. We demonstrated the utility, speed and accuracy of this approach on several datasets