105 research outputs found
Proposed physiologic functions of boron in plants pertinent to animal and human metabolism.
Boron has been recognized since 1923 as an essential micronutrient element for higher plants. Over the years, many roles for boron in plants have been proposed, including functions in sugar transport, cell wall synthesis and lignification, cell wall structure, carbohydrate metabolism, RNA metabolism, respiration, indole acetic acid metabolism, phenol metabolism and membrane transport. However, the mechanism of boron involvement in each case remains unclear. Recent work has focused on two major plant-cell components: cell walls and membranes. In both, boron could play a structural role by bridging hydroxyl groups. In membranes, it could also be involved in ion transport and redox reactions by stimulating enzymes like nicotinamide adenine dinucleotide and reduced (NADH) oxidase. There is a very narrow window between the levels of boron required by and toxic to plants. The mechanisms of boron toxicity are also unknown. In nitrogen-fixing leguminous plants, foliarly applied boron causes up to a 1000% increase in the concentration of allantoic acid in leaves. In vitro studies show that boron inhibits the manganese-dependent allantoate amidohydrolase, and foliar application of manganese prior to application of boron eliminates allantoic acid accumulation in leaves. Interaction between borate and divalent cations like manganese may alter metabolic pathways, which could explain why higher concentrations of boron can be toxic to plants
Incidence of hereditary amyloidosis and autoinflammatory diseases in Sweden: endemic and imported diseases
Septins restrict inflammation and protect zebrafish larvae from Shigella infection
Shigella flexneri, a Gram-negative enteroinvasive pathogen, causes inflammatory destruction of the human intestinal epithelium. Infection by S. flexneri has been well-studied in vitro and is a paradigm for bacterial interactions with the host immune system. Recent work has revealed that components of the cytoskeleton have important functions in innate immunity and inflammation control. Septins, highly conserved cytoskeletal proteins, have emerged as key players in innate immunity to bacterial infection, yet septin function in vivo is poorly understood. Here, we use S. flexneri infection of zebrafish (Danio rerio) larvae to study in vivo the role of septins in inflammation and infection control. We found that depletion of Sept15 or Sept7b, zebrafish orthologs of human SEPT7, significantly increased host susceptibility to bacterial infection. Live-cell imaging of Sept15-depleted larvae revealed increasing bacterial burdens and a failure of neutrophils to control infection. Strikingly, Sept15-depleted larvae present significantly increased activity of Caspase-1 and more cell death upon S. flexneri infection. Dampening of the inflammatory response with anakinra, an antagonist of interleukin-1 receptor (IL-1R), counteracts Sept15 deficiency in vivo by protecting zebrafish from hyper-inflammation and S. flexneri infection. These findings highlight a new role for septins in host defence against bacterial infection, and suggest that septin dysfunction may be an underlying factor in cases of hyper-inflammation
Carbon release from rice roots under paddy rice and maize-paddy rice cropping
Crop rotations encompassing flooded rice and an upland crop are commonly found in large parts of South and East Asia. However, also rice farmers in Southeast Asia increasingly switch from double-cropping paddy rice to one non-flooded cropâsuch as maizeâin the dry season. We hypothesized that introducing maize (maizeâpaddy rice, M-MIX) into a double paddy rice (R-WET) cropping system will increase carbon (C) release from rice roots into the rhizosphere and the dissolved soil C pool. To test this hypothesis we assessed the kinetics of C release by the rice plants in a hydroponic greenhouse experiment, and used these data for interpreting their C release in field experiments using 13C pulse labeling of rice plants. In the greenhouse we observed that rice roots released 13C labeled dissolved organic carbon (DOC) for 21 days with a mean residence time (MRT) of 19 days after exposure to a 13CO2 pulse. The MRT of labeled dissolved inorganic carbon (DIC) released from rice roots was only 2 days. In the field 13CO2 pulse labeling increased the 13C excess of rhizosphere soil up to 0.7 ± 0.2 mg 13C kgâ1 in R-WET and 0.9 ± 0.3 mg kgâ1 in M-MIX. The 13C signature of bulk soil remained unaffected. DOC concentrations in R-WET were significantly higher than in M-MIX during the mature grain stage of the rice plants. Nevertheless, the 13C excess in DOC transiently increased by only 0.5 ÎŒg Lâ1 after labeling in 13 cm depth in one of three lysimeters previously cropped with maize (M-MIX), while no labeled DOC was detected in 13 cm depth of the R-WET lysimeters and in 60 cm depth of both treatments. In contrast, the 13C excess of DIC increased by 42.4â93.1 ÎŒg Lâ1 a few days after labeling with a MRT of 53â66 days in both treatments. Considering the results of the greenhouse experiment, this suggests a rapid mineralization of labeled rhizodeposits in the field and an effective transient storage of CO2 produced by respiration in soil water
Cryopyrin-associated periodic syndrome in Australian children and adults: Epidemiological, clinical and treatment characteristics.
AIM: Cryopyrin-associated periodic syndromes (CAPS) encapsulate three auto-inflammatory conditions, ranging in severity from mild (familial cold auto-inflammatory syndrome: FCAS), moderate (Muckle-Wells syndrome: MWS) and severe (neonatal onset multi-inflammatory disorder: NOMID). We aimed to describe the epidemiology, clinical features and outcomes of Australian children and adults with CAPS. METHODS: Patients were identified and clinical data collected through a questionnaire sent during 2012-2013 to clinicians reporting to the Australian Paediatric Surveillance Unit and subscribing to the Australasian Societies for Allergy/Immunology, Rheumatology and Dermatology. RESULTS: Eighteen cases of CAPS were identified (8 NOMID; 8 MWS, 2 FCAS); 12 in children <18âyears of age. The estimated population prevalence of CAPS was 1 per million persons. Diagnostic delay was frequent, particularly in those with milder phenotypes (median diagnostic delay in MWS/FCAS 20.6âyears compared with NOMID 2.1âyears; Pâ=â0.04). Common presenting features included urticaria (100%), periodic fever (78%), arthralgia (72%) and sensorineural hearing loss (61%). Almost all (90%) MWS patients had a family member similarly affected compared with none in the NOMID group (Pâ=â0.004). A significant proportion of patients on anti-interleukin (IL)-1 therapy (nâ=â13) no longer had systemic inflammation. Only 50% with sensorineural hearing loss had hearing restored on anti-IL-1 therapy. CONCLUSIONS: Although CAPS are rare, patients often endured prolonged periods of systemic inflammation. This is despite almost all MWS patients having family members with similar symptoms and children with NOMID presenting with chronic infantile urticaria associated with multi-system inflammation. Hearing loss in NOMID/MWS was frequent, and reversible in only 50% of cases
Interaction of maternal environment and allelic differences in seed vigour genes determines seed performance in Brassica oleracea
Seed vigour is a key trait essential for the production of sustainable and profitable crops. The genetic basis of variation in seed vigour has recently been determined in Brassica oleracea, but the relative importance of the interaction with parental environment is unknown. We produced seeds under a range of maternal environments, including global warming scenarios. Lines were compared that had the same genetic background, but different alleles (for high and low vigour) at the quantitative trait loci responsible for determining seed vigour by altering abscisic acid (ABA) content and sensitivity. We found a consistent effect of beneficial alleles across production environments; however, environmental stress during production also had a large impact that enhanced the genetic difference in seed performance, measured as germination speed, resistance to controlled deterioration and induction of secondary dormancy. Environmental interaction with allelic differences in key genes that determine ABA content and sensitivity develops a continuity in performance from rapid germination through to failure to complete germination, and increasing depths of seed dormancy. The geneticâenvironmental interaction revealed provides a robust mechanism of betâhedging to minimize environmental risk during subsequent germination, and this could have facilitated the rapid change in seed behaviour (reduced dormancy and rapid germination) observed during crop domestication
Cryopyrin-associated periodic syndrome in Australian children and adults: Epidemiological, clinical and treatment characteristics
An Amphiphilic Ruthenium Polymetallodrug for Combined Photodynamic Therapy and Photochemotherapy In Vivo
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