A New Source of Natural Antioxidants From Turkey: Linaria GenistifoliaSubsp. Genistifolia

The antioxidant properties of different solvent extracts (acetone, methanol and water) from Linaria genistifolia subsp. genistifolia were investigated. Antioxidant properties were evaluated by different methods including free radical scavenging (DPPH and ABTS), reducing power (CUPRAC and FRAP), phosphomolybdenum, β-carotene/linoleic acid and metal chelating assays. Also, total phenolic and flavonoid content were determined for each extracts. Generally, the acetone and methanol extracts exhibited strong antioxidant abilities with higher level of phenolics (40.17 mgGAEs/g for acetone and 28.18 mgGAEs/g for methanol). All extracts had remarkable inhibition abilities of linoleic acid oxidation (84.88% for acetone, 77.22% for methanol and 63.04 for water). The results indicate that Linaria genistifolia subsp. genistifolia could be considered as a source of natural antioxidant for preparing new food ingredients and pharmaceutical formulations

Studies on in vitro antioxidant activities of nine diff erent fruit tree leaves collected from Mediterranean region of Turkey

To investigate antioxidant of the methanolic and water extracts obtained from nine different fruit tree leaves(avocado, walnut, mulberry, fig, carob, lemon, pomegranate, grape, and loquat) collected from Mediterraneanregion of Turkey. The antioxidant activities were evaluated with different in vitro antioxidant assays includingphosphomolybdate assay, free radical scavenging assays (•OH, NO, and O2−), β-carotene/linoleic acid testsystem, and ferric reducing power. The contents of saponin and tannin in extracts were also determined. Thepresent study suggests that the extracts were good radical scavengers. These results suggest that the extractsexamined should be beneficial as a source of natural agents for the food industry and pharmacological applications

INVESTIGATION OF ANTIOXIDANT POTENTIALS OF SOLVENT EXTRACTS FROM DIFFERENT ANATOMICAL PARTS OF ASPHODELINE ANATOLICA E. TUZLACİ: AN ENDEMIC PLANT TO TURKEY

Background: The genus Asphodeline (Liliaceae) is represented in Turkey by 20 taxa, which are traditionally used for medicinal purposes in Anatolia. Materials and Methods: In this study, we tested the phytochemical content and antioxidant effect of different solvent extracts obtained from different anatomical parts of Asphodeline anatolica. The different extracts of each plant parts were tested for antioxidant activity using different chemical assays. The total antioxidant components were also calculated. Results: Generally, acetone extracts produced the seed and root exhibited significantly higher antioxidant activity with high antioxidant components. Total phenolic content of extracts were significantly correlated with antioxidant potentials (except for, metal chelating activity). Conclusion: On the basis of the results obtained, A. anatolica extracts should be regarded as a valuable source of natural antioxidants for food and therapeutic applications

Fatty acid composition of fl owers of Crepis foetida subsp. rhoeadifolia from Turkey

This study aims to describe the fatty acid composition of flowers of Crepis foetida subsp. rhoeadifolia. In order todefine qualitative and quantitative profile, fatty acids were derived to their methyl esters, and then these wereanalyzed by gas chromatography - flame ionization detector. 20 fatty acids were determined in the oil. Palmitic(C 16:0), myristic (C 14:0), and linoleic acids (18:2 ω6) were found to be major fatty acids. Total saturatedfatty acids, monounsaturated fatty acids, and polyunsaturated fatty acids (PUFA) were 62.67%, 17.54%, and19.79%, respectively. Also, total essential fatty acids (EFA) were determined ad 14.48%. Therefore, the oil canbe considered as a new source of PUFA, especially EFA

Short-term wind speed forecasting system using deep learning for wind turbine applications

It is very important to accurately detect wind direction and speed for wind energy that is one of the essential sustainable energy sources. Studies on the wind speed forecasting are generally carried out for long-term predictions. One of the main reasons for the long-term forecasts is the correct planning of the area where the wind turbine will be built due to the high investment costs and long-term returns. Besides that, short-term forecasting is another important point for the efficient use of wind turbines. In addition to estimating only average values, making instant and dynamic short-term forecasts are necessary to control wind turbines. In this study, short-term forecasting of the changes in wind speed between 1-20 minutes using deep learning was performed. Wind speed data was obtained instantaneously from the feedback of the emulated wind turbine's generator. These dynamically changing data was used as an input of the deep learning algorithm. Each new data from the generator was used as both test and training input in the proposed approach. In this way, the model accuracy and enhancement were provided simultaneously. The proposed approach was turned into a modular independent integrated system to work in various wind turbine applications. It was observed that the system can predict wind speed dynamically with around 3% error in the applications in the test setup applications

Capacidad antioxidante y perfil de ácidos grasos de Centaurea kotschyi (Boiss. & Heldr.) Hayek var. persica (Boiss.) Wagenitz de Turquía

The antioxidant capacity of the methanolic extract and the fatty acid composition of C. kotschyi var. persica were investigated. Six different chemical methods were used to determine the antioxidant capacity. The fatty acid composition was analyzed using gas chromatography. The IC50 value of the extract was determined as 37.09 μg/ml (in the DPPH assay). In the β-carotene/linoleic acid system, the extract exhibited 65.22% inhibition against linoleic acid oxidation. The amount of total phenolic content and total antioxidant capacity were detected as 36.52 mg gallic acid equivalent (GAE)/g and 74.93 mg ascorbic acid equivalent (AE)/g, respectively. The major fatty acid in the composition of C. kotschyi var. persica was found to be C 18:3 ω3 (α-linolenic acid) by GC analysis. The results presented here indicate that C. kotschyi var. persica possess strong antioxidant properties. Therefore, the species can be used as a natural additive in food, cosmetic and pharmaceutical industries.La capacidad antioxidante de extractos metanólicos y composición de ácidos grasos de C. kotschyi var. pérsica fueron investigados. Seis métodos químicos diferentes fueron realizados para la determinación de la capacidad antioxidante. La composición de ácidos grasos fue analizada por cromatografía de gases. Los valores de IC50 de los extractos fueron 37.09 μg/ml (en el ensayo con DPPH). En el sistema β-carotene/ácido linoleico, el extracto mostró un 65.22% de inhibición frente a la oxidación del ácido linoleico. La cantidad total de contenido fenólico y capacidad antioxidante total fueron 36.52 mg equivalentes de ácido gallico (GAE)/g y 74.93 mg equivalentes de ácido ascórbico (AE)/g, respectivamente. El principal ácidos graso encontrado, por análisis de CG, en C. kotschyi var. pérsica fue el C 18:3 ω3 (ácido α-linolenico). Los resultados presentados aquí indican que C. kotschyi var. pérsica posee unas fuertes propiedades antioxidantes. Además, las especies pueden ser usadas como aditivos naturales en los alimentos, en cosmética y en industria farmacéutica

Discovery of orexant and anorexant agents with indazole scaffold endowed with peripheral antiedema activity

CB1 receptors and endocannabinoids are integrated components of neuronal networks controlling different organism’s functions, such as appetite and food intake in the hypothalamus. A series of Rimonabant/Fubinaca hybrids have been synthesized in solution as C-terminal amides, acids, methyl esters and N-methyl amides. These compounds have been studied in cannabinoid receptor binding assay and functional receptor assay in vitro, the most active among them as agonist (LONI 11) and antagonist (LONI 4) were tested in vivo to evaluate their ability to stimulate or suppress the feeding behavior after i.p. administration. For LONI 11 formalin test and tail flick tests after s.c. and i.c.v. routes respectively, were also performed in vivo with the aim to investigate the antinociceptive effect at the central or peripheral level. In the Zymosan-induced edema and hyperalgesia, LONI 11 reduced the % paw volume increase and % paw latency after s.c. administration, also suggesting a potential anti-inflammatory activity at the periphery. Keywords. Cannabinoid receptor, Rimonabant, food intake, anorexant agent, edema

In vitro studies on different extracts of fenugreek (Trigonella spruneriana BOISS.): Phytochemical profile, antioxidant activity, and enzyme inhibition potential

© 2020 Wiley Periodicals LLC. The side effects of synthetic antioxidants make it necessary to find a natural alternative. Therefore, the current study investigates the potential of T. spruneriana as a new alternative in terms of natural bioactive components. In this context, antioxidant activity, enzyme inhibition, and phenolic compounds of different extracts including ethanol, methanol, ethyl acetate, and aqueous were identified. The results show that the ethyl acetate (113.59 ± 2.73 mg GAE/g) has the highest phenolic content, but ethanol extract has the highest scavenging activity for DPPH and TAC. The ethanol extract showed stronger inhibition on cholinesterase and α-amylase compared to other extracts. Besides, 12 bioactive compounds were characterized in T. spruneriana extracts by HPLC-DAD. Our findings support that T. spruneriana could be considered as a new source of active phytochemicals, as well as provide remarkable data on biological activities of some main enzymes playing role in the healing of hyperpigmentation, Alzheimer, and diabetes. Practical applications: This study reports the total content, types and amounts of bioactive compounds and potential beneficial bioactivities of the different extracts of T. spruneriana. Trigonella is abundant in nature and spread over a wide geographical area, and is used in making cheese, pastries, spices, and sausages in different countries, as well as for antidiabetic purposes. Trigonella leaves are a good source of bioactive compounds that contain compounds like quercetin, catechin, cinnamic acid, and coumaric acid, along with it have also a high content of soluble fibers and is suggested for body weight control. Apart from being the first study conducted to point out the potential of T. spruneriana as being a natural food additive, this study also demonstrated its medicinal importance by revealing the anti-hyperpigmentation, antidiabetic, neuroprotective, and antioxidant properties of T. spruneriana.info:eu-repo/semantics/publishedVersio

Chemical composition and biological activity of Capparis spinosa L. from Lipari Island

Several plants belonging to the genus Capparis are the focus of growing interest due to their singular nutritional and medicinal properties. In the present study, flower bud samples from C. spinosa L. (Lipari Island, Italy) were subjected to decoction, Soxhlet, and microwave extraction techniques and the individual extracts investigated to better characterize the phytochemical and antioxidant profiles of the plant. Total phenolic and flavonoid amounts, phenolic composition, radical scavenging as well as reductive and metal chelating properties were determinated by well-established chemical and analytical procedures. Furthermore, cholinesterase inhibitory effects were evaluated by Ellman's method. Fatty acid percentage and essential oil composition were also detected by GC and GC-MS techniques respectively. Rutin was found to be the major component in the studied extracts. The Soxhlet extract exhibited the strongest radical scavenging and reductive activities as compared to the other extracts, most probably due to the highest concentration of phenolics, especially rutin. The best cholinesterase inhibitory effect was observed in the microwave extract. Palmitic acid was the most abundant fatty acid in the studied oil, whereas docosane was the major volatile compound in the essential oil. Present data corroborate the multipurpose potential of C. spinosa for designing bio-based drug formulations or functional applications. (c) 2018 SAAB. Published by Elsevier B.V. All rights reserved

Selectivity Tuning by Natural Deep Eutectic Solvents (NADESs) for Extraction of Bioactive Compounds from Cytinus hypocistis—Studies of Antioxidative, Enzyme-Inhibitive Properties and LC-MS Profiles

In the present study, the extracts of Cytinus hypocistis (L.) L using both traditional solvents (hexane, ethyl acetate, dichloromethane, ethanol, ethanol/water, and water) and natural deep eutectic solvents (NADESs) were investigated in terms of their total polyphenolic contents and antioxidant and enzyme-inhibitive properties. The extracts were found to possess total phenolic and total flavonoid contents in the ranges of 26.47–186.13 mg GAE/g and 0.68–12.55 mg RE/g, respectively. Higher total phenolic contents were obtained for NADES extracts. Compositional differences were reported in relation to antioxidant potential studied by several assays (DPPH: 70.19–939.35 mg TE/g, ABTS: 172.56–4026.50 mg TE/g; CUPRAC: 97.41–1730.38 mg TE/g, FRAP: 84.11–1534.85 mg TE/g). Application of NADESs (choline chloride—urea 1:2, a so-called Reline) allowed one to obtain the highest number of extracts having antioxidant potential in the radical scavenging and reducing assays. NADES-B (protonated by HCl L-proline-xylitol 5:1) was the only extractant from the studied solvents that isolated a specific fraction without chelating activity. Reline extract exhibited the highest acetylcholinesterase inhibition compared to NADES-B and NADES-C (protonated by H2SO4 L-proline-xylitol 5:1) extracts, which showed no inhibition. The NADES extracts were observed to have higher tyrosinase inhibitory properties compared to extracts obtained by traditional organic solvents. Furthermore, the NADES extracts were relatively better inhibitors of the diabetic enzymes. These findings provided an interesting comparison in terms of total polyphenolic content yields, antioxidant and enzyme inhibitory properties (cholinesterase, amylase, glucosidase, and tyrosinase) between traditional solvent extracts and NADES extracts, used as an alternative. While the organic solvents showed better antioxidant activity, the NADES extracts were found to have some other improved properties, such as higher total phenolic content and enzyme-inhibiting properties, suggesting functional prospects for their use in phytonutrient extraction and fractionation. The obtained results could also be used to give a broad overview of the different biological potentials of C. hypocistis.National Science Centre, Poland UMO-2018/30/E/ST8/0064