Graduates of Character - Values and Character: Higher Education and Graduate Employment

Graduates of Character is the product of an empirical enquiry into the values, virtues, dispositions and attitudes of a sample of students and employees who volunteered to be involved. The research team sought host sites which would offer a diverse set of interviewees in gender, ethnicity, religion and aspiration. In this study we discuss what character is taken to mean by students and employees in their years of higher education and employment. We examine what their values are, what they gain from the university, what they believe employers look for when recruiting, what they hope to give to an employer, and what they expect from their employer. We then explore who or what influenced their values and moral development. We also examined the role of the personal tutor or mentor, and the persons or services to which they might go for personal and/or professional support

Lentiviral manipulation of gene expression in human adult and embryonic stem cells

Human stem cells could revolutionize the field of medicine by providing a diverse range of cell types for tissue replacement therapies and drug discovery. To achieve this goal, genetic tools need to be optimized and developed for controlling and manipulating stem cells ex vivo. Here we describe a lentiviral delivery system capable of high infection rates in human mesenchymal and embryonic stem cells. The lentiviral backbone was modified to express mono- and bi-cistronic transgenes and was also used to deliver short hairpin ribonucleic acid for specific silencing of gene expression in human stem cells. We show that lentiviral transduction can be used to alter gene expression without altering the genes' ability to differentiate in vitro. These vectors will enable rapid analysis of gene function in stem cells and permit the generation of knock-in / knock-out models of human disease in the rapidly developing field of gene therapy

Calculating Great Britains half-hourly electrical demand from publicly available data

Here we present a method to combine half-hourly publicly available electrical generation and interconnector data to create a timeseries that approximates Great Britains electrical demand. Publishing the method and the data provides a resource to the wider community that can be further enhanced or adapted and allows the method itself to be considered and critiqued. The method adds value by combining transmission and distribution generation data into a single dataset and adding ISO 8601 compatible datetimes to increase interoperability with other data. The published data is therefore more useable by a wider group of researchers and stakeholders interested in an example of the rapid decarbonisation of a countries electrical system.Comment: 33 pages, 3 Figures, 6 table

cDNA that encodes active agrin

Agrin is thought to mediate the motor neuron-induced aggregation of AChRs and AChE on the surface of muscle fibers at neuromuscular junctions. We have isolated a cDNA from a chick brain library that, based on sequence homology and expression experiments, codes for active agrin. Examination of the sequence reveals considerable similarity to homologous cDNAs previously isolated from ray and rat libraries. A conspicuous difference is an insertion of 33 by in chick agrin cDNA, which endows the encoded protein with AChR/AChE aggregating activity. Homologous transcripts having the 33 by insertion were detected in the ray CNS, which indicates that an insertion of similar size is conserved in agrin in many, if not all, vertebrate species. Results of in situ hybridization studies and PCR experiments on mRNA isolated from motor neuron-enriched fractions of the spinal cord indicate that, consistent with the agrin hypothesis, motor neurons contain transcripts that code for active agrin

What is a hologenomic adaptation? Emergent individuality and inter-identity in multispecies systems

Contemporary biological research has suggested that some host–microbiome multispecies systems (referred to as “holobionts”) can in certain circumstances evolve as unique biological individual, thus being a unit of selection in evolution. If this is so, then it is arguably the case that some biological adaptations have evolved at the level of the multispecies system, what we call hologenomic adaptations. However, no research has yet been devoted to investigating their nature, or how these adaptations can be distinguished from adaptations at the species-level (genomic adaptations). In this paper, we cover this gap by investigating the nature of hologenomic adaptations. By drawing on the case of the evolution of sanguivory diet in vampire bats, we argue that a trait constitutes a hologenomic adaptation when its evolution can only be explained if the holobiont is considered the biological individual that manifests this adaptation, while the bacterial taxa that bear the trait are only opportunistic beneficiaries of it. We then use the philosophical notions of emergence and inter-identity to explain the nature of this form of individuality and argue why it is special of holobionts. Overall, our paper illustrates how the use of philosophical concepts can illuminate scientific discussions, in the trend of what has recently been called metaphysics of biology

Developing personal relationships in care homes: realising the contributions of staff, residents and family members

Personal relationships are all integral part of living, working and visiting in care homes, but little research has made relationships the main focus of enquiry, and there have been few studies of the perspectives of residents, staff and family members. The study reported here sought to redress this neglect. Using a constructivist approach, the nature and types of relationships between residents, staff and family members were explored in three care homes in England using combined methods including participant observation, interviews and focus groups. The data collection and analysis Occurred iteratively Over 21 months and three types of relationships were identified: 'pragmatic relationships' that primarily focus oil the instrumental aspects of care; 'personal and responsive relationships' that engage more fully with the particular needs of individual residents; and 'reciprocal relationships' that recognise the roles of residents, staff and family members in creating a sense of community within the home. This paper explores the contributions made by staff; residents and family members in the development of these relationships. The findings enhance our understanding of the role of inter-personal relationships in care home settings and of the factors that condition them. The implications for developing improved practice in care ponies are also considered

Fate of sulphate removed during the treatment of circumneutral mine water and acid mine drainage with coal fly ash: Modelling and experimental approach

The treatment of acid mine drainage (AMD) and circumneutral mine water (CMW) with South African coal fly ash (FA) provides a low cost and alternative technique for treating mine wastes waters. The sulphate concentration in AMD can be reduced significantly when AMD was treated with the FA to pH 9. On the other hand an insignificant amount of sulphate was removed when CMW (containing a very low concentration of Fe and Al) was treated using FA to pH 9. The levels of Fe and Al, and the final solution pH in the AMD–fly ash mixture played a significant role on the level of sulphate removal in contrast to CMW–fly ash mixtures. In this study, a modelling approach using PHREEQC geochemical modelling software was combined with AMD–fly ash and/or CMW–fly ash neutralization experiments in order to predict the mineral phases involved in sulphate removal. The effects of solution pH and Fe and Al concentration in mine water on sulphate were also investigated. The results obtained showed that sulphate, Fe, Al, Mg and Mn removal from AMD and/or CMW with fly ash is a function of solution pH. The presence of Fe and Al in AMD exhibited buffering characteristic leading to more lime leaching from FA into mine water, hence increasing the concentration of Ca2+. This resulted in increased removal of sulphate as CaSO4·2H2O. In addition the sulphate removal was enhanced through the precipitation as Fe and Al oxyhydroxysulphates (as shown by geochemical modelling) in AMD–fly ash system. The low concentration of Fe and Al in CMW resulted in sulphate removal depending mainly on CaSO4·2H2O. The results of this study would have implications on the design of treatment methods relevant for different mine waters.Web of Scienc

A search for 85.5- and 86.6-GHz methanol maser emission

We have used the Australia Telescope National Facility Mopra 22m millimetre telescope to search for emission from the 85.5-GHz and 86.6-GHz transitions of methanol. The search was targeted towards 22 star formation regions which exhibit maser emission in the 107.0-GHz methanol transition, as well as in the 6.6-GHz transition characteristic of class II methanol maser sources. A total of 22 regions were searched at 85.5 GHz resulting in 5 detections, of which 1 appears to be a newly discovered maser. For the 86.6-GHz transition observations were made of 18 regions which yielded 2 detections, but no new maser sources. This search demonstrates that emission from the 85.5- and 86.6-GHz transitions is rare. Detection of maser emission from either of these transitions therefore indicates the presence of special conditions, different from those in the majority of methanol maser sources. We have observed temporal variability in the 86.6-GHz emission towards 345.010+1.792, which along with the very narrow line width, confirms that the emission is a maser in this source. We have combined our current observations with published data for the 6.6-, 12.1-, 85.5-, 86.6-, 107.0-, 108.8- and 156.6-GHz transitions for comparison with the maser model of Sobolev & Deguchi (1994). This has allowed us to estimate the likely ranges of dust temperature, gas density, and methanol column density, both for typical methanol maser sources and for those sources which also show 107.0-GHz emission.Comment: 11 pages, accepted for publication in MNRAS, Latex, mn2e.cl

Chandra Observations of the Radio Galaxy 3C 445 and the Hotspot X-ray Emission Mechanism

We present new {\it Chandra} observations of the radio galaxy 3C 445, centered on its southern radio hotspot. Our observations detect X-ray emission displaced upstream and to the west of the radio-optical hotspot. Attempting to reproduce both the observed spectral energy distribution (SED) and the displacement, excludes all one zone models. Modeling of the radio-optical hotspot spectrum suggests that the electron distribution has a low energy cutoff or break approximately at the proton rest mass energy. The X-rays could be due to external Compton scattering of the cosmic microwave background (EC/CMB) coming from the fast (Lorentz factor $\Gamma\approx 4$) part of a decelerating flow, but this requires a small angle between the jet velocity and the observer's line of sight ($\theta\approx 14^{\circ}$). Alternatively, the X-ray emission can be synchrotron from a separate population of electrons. This last interpretation does not require the X-ray emission to be beamed.Comment: 9 pages, 5 figures, ApJ, in pres