A survey on pest insects of fiber and grain sorghum in northern Italy

In the Po valley plain grain sorghum (Sorghum bicolor) has been introduced in the last decades for cattle feed production. More recently fiber sorghum has been tested to be used for electric power production. Since little is known about the sorghum insect pest community in northern Italy, a research was carried out in a study area located in the central western area of the Po valley. Grain sorghum plots and fields were studied between 2008 and 2011. No insect pest of economic importance was recorded in grain sorghum cultivations. Population density of the aphid Rhopalosiphim maidis was very low, as well as the one of the European Corn Borer (ECB, Ostrinia nubilalis, Lepidoptera Crambidae). Lygus rugulipennis (Hemiptera Miridae) tended to colonize grain sorghum after panicle development, but densities were not a matter of concern. In fiber sorghum plots ECB populations did not result noxious, while R. maidis density was higher than the one on grain sorghum, but infestations did not signifi¬cantly affect the development of the stalks, being localized only on panicles during their first stage of develop¬ment. The production of cyanogenetic compounds by young sorghum plants is likely to be an effective protection against ECB first generation larvae, while earlier harvest time of both grain and fiber sorghum compared with maize later harvest time prevents significant damages caused by ECB second generation larvae. Sorghum requires less water than maize. Such a condition, joint to the weak susceptibility to insect pests, could favour a further increase of sorghum cultivations in northern Italy

On-sight and red-point climbing: changes in performance and route-finding ability in male advanced climbers

Aim: In lead climbing, the ascent of the route can be defined as on-sight or red-point. On-sight is the more challenging style since it demands greater physiological and psychological commitment. The differences between the two modes in advanced climbers have not been studied much. Two essential skills needed to optimize performance, in both on-sight and in red-point climbing, are route interpretation (RI) ability and movements sequence recall. Therefore, this study aimed to compare performance between on-sight and red-point ascent in advanced climbers and evaluate how a climber’s RI ability and movement sequences recall might change before and after on-sight and red-point climbing. Methods: Eighteen advanced male climbers (age 29.2 ± 4.7 years, body mass 67.8 ± 3.6 kg, stature 175.2 ± 2.4 cm, best red-point and on-sight grades 7b+/8a and 7a+/7b+, respectively) were video-recorded during the route ascent in on-sight and red-point modes to evaluate performance and to measure static and dynamic action times. RI ability and movement sequence recall were assessed before and after each climb. Level of anxiety was evaluated via a self-report questionnaire. Heart rate (fH), lactate concentration, ([La–]), and rating of perceived exertion (RPE) were detected during and after each climb. Results: Compared to on-sight, an improvement in performance was observed in a red-point climb: the ascent was faster (148.7 ± 13.6 s and 179.5 ± 12.5 s, respectively, P < 0.05), smoother (significant reduction in exploratory moves and in stops times, P < 0.05), less demanding physiologically (lower fHpeak and [La–]peak, P < 0.05), and psychologically (lower RPE, cognitive and somatic anxiety and higher self-confidence, P < 0.05). The RI ability was improved in red-point versus on-sight and, in the same mode, between pre and post ascent. Conclusion: Red-point climbing was found to be less demanding than on-sight, both physiologically and psychologically, under the conditions investigated by this study. Our findings suggest that RI is a trainable skill and underscore the importance of including specific techniques in training programs designed to improve interaction between perceptual, psychological, and physiological factors

Cardiorespiratory and Metabolic Responses to Sinusoidal Exercise of Moderate Intensity: Reliability of the Measurements and the Effects of Fatigue

The cardiorespiratory and metabolic responses to sinusoidal exercise, in which work rate follows an oscillating pattern, have been proposed to assess the effectiveness of the cardiorespiratory adjustments. The repetition of successive sinusoidal periods permits to simultaneously reduce the influence of random fluctuations and accentuate the underlying physiological response. Data analysis has been often performed by overlapping and averaging successive cycles assuming no differences among them, thus excluding the possible presence of fatigue throughout successive cycles. After assessing the reliability of the measurements during sinusoidal exercise, this study sought to investigate the possible differences among subsequent cycles of sinusoidal work. Eleven active volunteers (age: 28±6 yrs., body mass: 73±7 kg; stature: 1.79±0.06 m, maximum oxygen uptake (VO2max): 52 ml·kg−1·min−1) participated to the study that was conducted in accordance with the Basic Principles of the Declaration of Helsinki. After determining individual VO2max and critical power (CP) on a cycle ergometer, they underwent sinusoidal work rates characterized by an amplitude (A), a midpoint (MP) and a period equal to ±50W, 50W below CP and 240s, respectively, up to exhaustion. On a different day, participants repeated the same experimental session for reliability purposes. Expiratory ventilation (VE), oxygen uptake (VO2), carbon dioxide output (VCO2), and heart rate (fH) responses were fitted by the sinewave function that minimized the residuals. A, MP and the time-delay (tD, the latency between mechanical work rate and physiological responses) of all parameters were determined for each cycle. Reliability assessment between day 1 and 2 was expressed as Cronbach’s a and intraclass correlation coefficient (ICC). A one-way ANOVA for repeated measures tested the presence of differences among cycles. Regression analysis was also applied to explore possible relationship between each variables and time. Reliability analysis revealed a very high to high ICC values for most of the parameters, with the exception of A for VO2 and VCO2 and tD for fH (moderate reliability). A of VE and fH response increased and decreased with time, respectively (p<0.05). MP of VE and fH showed a positive regression that led to significantly higher values in the last compared to the first cycle; on the contrary, no changes were observed among cycles in all other MP data. tD was similar in each cycle for all the investigated parameters despite a very slight negative regression found for VCO2. In conclusion, most of the physiological responses to moderate sinusoidal exercise exhibited a high to very high reliability. Some of the cardiorespiratory parameters showed significant changes with time throughout the sinusoidal exercise possibly due to the onset of fatigue. Therefore, an approach that overlaps and averages all the cycles together should not be performed to avoid wrong estimation of physiological responses to sinusoidal exercise, unless the averaging approach involves only the first cycles

Dioxin Exposure, from Infancy through Puberty, Produces Endocrine Disruption and Affects Human Semen Quality

BACKGROUND: Environmental toxicants are allegedly involved in decreasing semen quality in recent decades; however, definitive proof is not yet available. In 1976 an accident exposed residents in Seveso, Italy, to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). OBJECTIVE: The purpose of this study was to investigate reproductive hormones and sperm quality in exposed males. METHODS: We studied 135 males exposed to TCDD at three age groups, infancy/prepuberty (1-9 years), puberty (10-17 years), and adulthood (18-26 years), and 184 healthy male comparisons using 1976 serum TCDD levels and semen quality and reproductive hormones from samples collected 22 years later. RESULTS: Relative to comparisons, 71 men (mean age at exposure, 6.2 years; median serum TCDD, 210 ppt) at 22-31 years of age showed reductions in sperm concentration (53.6 vs. 72.5 million/mL; p = 0.025); percent progressive motility (33.2% vs. 40.8%; p < 0.001); total motile sperm count (44.2 vs. 77.5 x 10(6); p = 0.018); estradiol (76.2 vs. 95.9 pmol/L; p = 0.001); and an increase in follicle-stimulating hormone (FSH; 3.58 vs. 2.98 IU/L; p = 0.055). Forty-four men (mean age at exposure, 13.2 years; median serum TCDD, 164 ppt) at 32-39 years of age showed increased total sperm count (272 vs. 191.9 x 10(6); p = 0.042), total motile sperm count (105 vs. 64.9 x10(6); p = 0.036), FSH (4.1 vs. 3.2 UI/L; p = 0.038), and reduced estradiol (74.4 vs. 92.9 pmol/L; p < 0.001). No effects were observed in 20 men, 40-47 years of age, who were exposed to TCDD (median, 123 ppt) as adults (mean age at exposure, 21.5 years). CONCLUSIONS: Exposure to TCDD in infancy reduces sperm concentration and motility, and an opposite effect is seen with exposure during puberty. Exposure in either period leads to permanent reduction of estradiol and increased FSH. These effects are permanent and occur at TCDD concentrations < 68 ppt, which is within one order of magnitude of those in the industrialized world in the 1970s and 1980s and may be responsible at least in part for the reported decrease in sperm quality, especially in younger men

Leuprorelin Acetate Long-Lasting Effects on GnRH Receptors of Prostate Cancer Cells: An Atomic Force Microscopy Study of Agonist/Receptor Interaction

High cell-surface GnRH receptor (GnRH-R) levels have been shown to have a major influence on the extent of GnRH agonist-mediated tumor growth inhibition. The ability of the GnRH agonist leuprorelin acetate (LA) to induce a post-transcriptional upregulation of GnRH-R at the plasma membrane of androgen-sensitive (LNCaP) and -insensitive (PC-3) prostate cancer (PCa) cells has been previously demonstrated by Western blotting. Here we performed single molecule force spectroscopy by using Atomic Force Microscopy (AFM), which has proven to be a powerful tool allowing for investigation of living cell surface biological features, such as the so far unclear GnRH agonist/receptor interaction. Thus, in the hormone-insensitive PC-3 cells, we characterized the strength of the LA-receptor binding, and the amount and distribution of the functional receptor molecules on the cell surface. The effect of a long and continuous treatment (up to 30 days) with the agonist (10-11 and 10-6 M) on the same parameters was also investigated. A GnRH-R increase was observed, reaching the maximum (~80%) after 30 days of treatment with the highest dose of LA (10-6 M). The analogue-induced increase in GnRH-R was also demonstrated by Western blotting. In addition, two different receptor bound strengths were detected by AFM, which suggests the existence of two GnRH-R classes. A homogeneous distribution of the unbinding events has been found on untreated and treated PC-3 cell surfaces. The persistence of high receptor levels at the membrane of these living cells may warrant the maintenance of the response to LA also in androgen-unresponsive PCa. Moreover, the determination of ligand/receptor bond strength could shed light on the poorly understood event of LA/GnRH-R interaction and/or address structural/chemical agonist optimizations. \ua9 2013 Lama et al

Influence of Antisynthetase Antibodies Specificities on Antisynthetase Syndrome Clinical Spectrum TimeCourse

Introduction: Increased cardiovascular (CV) morbidity and mortality is observed in inflammatory joint diseases (IJDs) such as rheumatoid arthritis, ankylosing spondylitis, and psoriatic arthritis. However, the management of CV disease in these conditions is far from being well established.Areas covered: This review summarizes the main epidemiologic, pathophysiological, and clinical risk factors of CV disease associated with IJDs. Less common aspects on early diagnosis and risk stratification of the CV disease in these conditions are also discussed. In Europe, the most commonly used risk algorithm in patients with IJDs is the modified SCORE index based on the revised recommendations proposed by the EULAR task force in 2017.Expert opinion: Early identification of IJD patients at high risk of CV disease is essential. It should include the use of complementary noninvasive imaging techniques. A multidisciplinary approach aimed to improve heart-healthy habits, including strict control of classic CV risk factors is crucial. Adequate management of the underlying IJD is also of main importance since the reduction of disease activity decreases the risk of CV events. Non-steroidal anti-inflammatory drugs may have a lesser harmful effect in IJD than in the general population, due to their anti-inflammatory effects along with other potential beneficial effects.This research was partially funded by FOREUM—Foundation for Research in Rheumatolog

Racial differences in systemic sclerosis disease presentation: a European Scleroderma Trials and Research group study

Objectives. Racial factors play a significant role in SSc. We evaluated differences in SSc presentations between white patients (WP), Asian patients (AP) and black patients (BP) and analysed the effects of geographical locations.Methods. SSc characteristics of patients from the EUSTAR cohort were cross-sectionally compared across racial groups using survival and multiple logistic regression analyses.Results. The study included 9162 WP, 341 AP and 181 BP. AP developed the first non-RP feature faster than WP but slower than BP. AP were less frequently anti-centromere (ACA; odds ratio (OR) = 0.4, P &lt; 0.001) and more frequently anti-topoisomerase-I autoantibodies (ATA) positive (OR = 1.2, P = 0.068), while BP were less likely to be ACA and ATA positive than were WP [OR(ACA) = 0.3, P &lt; 0.001; OR(ATA) = 0.5, P = 0.020]. AP had less often (OR = 0.7, P = 0.06) and BP more often (OR = 2.7, P &lt; 0.001) diffuse skin involvement than had WP.AP and BP were more likely to have pulmonary hypertension [OR(AP) = 2.6, P &lt; 0.001; OR(BP) = 2.7, P = 0.03 vs WP] and a reduced forced vital capacity [OR(AP) = 2.5, P &lt; 0.001; OR(BP) = 2.4, P &lt; 0.004] than were WP. AP more often had an impaired diffusing capacity of the lung than had BP and WP [OR(AP vs BP) = 1.9, P = 0.038; OR(AP vs WP) = 2.4, P &lt; 0.001]. After RP onset, AP and BP had a higher hazard to die than had WP [hazard ratio (HR) (AP) = 1.6, P = 0.011; HR(BP) = 2.1, P &lt; 0.001].Conclusion. Compared with WP, and mostly independent of geographical location, AP have a faster and earlier disease onset with high prevalences of ATA, pulmonary hypertension and forced vital capacity impairment and higher mortality. BP had the fastest disease onset, a high prevalence of diffuse skin involvement and nominally the highest mortality

Impact of Chemical Insecticide Application on Beneficial Insects in Maize

The European corn borer (ECB) (Ostrinia nubilalis Hübner) and to a lesser extent the western corn rootworm (Diabrotica virgifera virgifera LeConte) are a threat to maize in the Po Valley (Northern Italy), and their control can require insecticide applications. The results of a study to evaluate the effects of insecticide sprays on the beneficial insect Trichogramma brassicae (Hymenoptera: Trichogrammatidae) and aphid predators are reported. A three-year research project was carried out in two Study Areas, in Lombardy. In area 1, crop rotation was a common practice, while in area 2 repeated maize crop was practiced. The natural trend of ECB egg masses attacked by T. brassicae was affected and parasitism rates were reduced as a result of insecticide exposure (chlorpyriphos methyl, cypermethrin, alphacypermethrine). Repeated maize crop and insecticides spraying increased the abundance of the aphid population and negatively affected the aphid predator community, which mainly included ladybirds, hoverflies, true bugs and lacewings. The predator community was dominated by hoverflies in sprayed fields managed according to repeated maize crop protocols, whereas ladybirds and Orius spp. dominated in maize fields managed according to crop rotation protocols. Crop rotation protocols help to prevent ECB outbreaks; when the risk of exceeding the economic threshold limit is high, and this may be the case when maize is cultivated for seeds or for horticultural crops such as sweet corn, inundative release of T. brassicae and/or microbial control (i.e., use of Bacillus thuringiensis preparations) can integrate natural biocontrol, and provide a valuable alternative to chemical insecticides

Sorveglianza di microrganismi “sentinella” all’Ospedale di Desio (MI) negli anni 2000-2005

Introduction: Antimicrobial resistance among nosocomial pathogens is steadily increasing, causing complications to patients and raising health care costs.To prevent the diffusion of antimicrobial-resistant organisms, a surveillance program was conducted at Desio Hospital.The aim of this work was to evaluate the prevalence of alert organisms in patients from Seregno and Desio Hospitals. Methods: Among alert organisms collected from 2000 to 2005, we considered: methicillin-resistant Staphylococcus aureus (MRSA), Clostridium difficile toxigenic (CD-tox), vancomycin-resistant Enterococci (VRE), pathogens producing Extended-Spectrum β-Lactamases (ESBLs).The prevalence was calculated as percentage of alert organisms on positive samples; CD-tox percentage is referred to all the samples collected. Rates for thousand inpatients were also calculated. Results: During the period 2000 to 2005 the prevalence of MRSA decreased from 61.3% to 44.9% (Pfortrend&lt;0.001).The rate/1000 of CD-tox increased from 0.6 to 3.5, although the prevalence fell from 23.1% to 17.8% (Pfortrend=0.003), owing to the growing number of requests.The prevalence of VRE decreased from 8.2% in 2003 to 2.3% in 2004 (not statistically significant).The prevalence of ESBLs increased from 3.8% in 2000 to 6.6% in 2005 (Pfortrend=0.025). Conclusions: The surveillance program conducted at Desio Hospital may have contributed in decreasing the number of MRSA,VRE and CD-tox isolates.The great increase in the frequency of ESBLs isolates is attested in different studies