Progesterona en leche, 2. Parámetros reproductivos evaluados mediante el análisis de progesterona en leche.

Se estudió en un grupo de 44 vacas la correlación de los perfiles típicos y atípicos, anestros e índices de preñez, diagnóstico precoz de preñez, incidencia de inseminaciones y ovulaciones perdidas, mediante la determinación de progesterona en leche por radioinmunoanálisis. Estos análisis se hicieron en el laboratorio de hormonas del Instituto de Asuntos Nucleares. Se estableció un criterio para definir si un perfil progestacional era típico o atípico, encontrando que las vacas con perfil progestacional tienden a presentar menos días abiertos. El promedio de días abiertos para las vacas con perfil progestacional típico fue de 161 y para aquellas con perfil progestacional atípico fue de 310. La duración del período anestro no difirió notablemente entre vacas preñadas y no preñadas. El índice de precisión para el diagnóstico precoz (21 días) de preñez fue positivo del 78 por ciento, mientras que para el negativo (vacas vacias) fue del 99 por ciento. El día post-inseminación más adecuado para la toma de muestra utilizada en este diagnóstico de preñez fue el día 21, aunque la diferencia no fue significativa con referencia a los días 19 y 23. El método permitió medir el número de inseminaciones y ovulaciones perdidasGanado de leche-Ganadería lech

Progesterona en leche, 1. Radio inmuno análisis de progesterona en leche.

Se desarrolló un método de análisis de progesterona en leche por RIA. En la estandarización de la técnica el tiempo de incubación antígeno-anticuerpo se redujo a 10 minutos. La leche dentro del sistema actuó como ligante no específico de la progesterona. Este efecto fue superado mediante la escogencia de una alicuota (10 ul) adecuada y la correlación introducida en la curva de calibración al adicionarle leche sin hormona detectable. La alícuota de 10 ul mencionada anteriormente contiene cantidades medibles dentro de los límites de máxima resolución del método (6,25 - 400 pg 0.1 ml). El uso de una suspensión de carbón-dextrano para la separación de la fracción libre y ligada no pudo ser sustituido por el de un segundo antisuero. Los valores en la leche se correlacionaron en forma altamente significativa con la existente en la sangre, no obstante, las concentraciones en leche fueron 5,2 veces más altas que en sangre. Estas dos circunstancias han ampliado la aplicabilidad y sensibilidad del método. Mediante análisis estadístico de datos allegados durante dos años, se comprobó la sensibilidad reproductiva y precisiónGanado de leche-Ganadería lech

Prevalencia en nuestro medio de las deformidades dinámicas posicionales del hallux como secuela de las fracturas de tercio medio y distal de tibia

Dynamic positional deformity of the hallux is a relatively new disease, precisely because of its innovative nature and dynamics may go unnoticed to clinicians. It is because of this, that the limited literature available stresses the importance of making an active search for this pathology in our daily practice. The purpose of this study is to estimate the prevalence of these deformities at the University Hospital Dr. Peset of Valencia as well as make an analysis of their management. An analytical, descriptive and retrospective study was designed in which patients admitted in our center during 2014 and 2015 with medium and distal third tibia fracture were analyzed searching for dynamics positional deformities of the hallux. We found a total of 5 compatible cases from 42 analyzed tibia fractures. This may indicate that as expected dynamic positional deformity of the hallux could be an underrated disease in our medium

EcoCyc: A comprehensive view of Escherichia coli biology

EcoCyc (http://EcoCyc.org) provides a comprehensive encyclopedia of Escherichia coli biology. EcoCyc integrates information about the genome, genes and gene products; the metabolic network; and the regulatory network of E. coli. Recent EcoCyc developments include a new initiative to represent and curate all types of E. coli regulatory processes such as attenuation and regulation by small RNAs. EcoCyc has started to curate Gene Ontology (GO) terms for E. coli and has made a dataset of E. coli GO terms available through the GO Web site. The curation and visualization of electron transfer processes has been significantly improved. Other software and Web site enhancements include the addition of tracks to the EcoCyc genome browser, in particular a type of track designed for the display of ChIP-chip datasets, and the development of a comparative genome browser. A new Genome Omics Viewer enables users to paint omics datasets onto the full E. coli genome for analysis. A new advanced query page guides users in interactively constructing complex database queries against EcoCyc. A Macintosh version of EcoCyc is now available. A series of Webinars is available to instruct users in the use of EcoCyc

Human diet, mobility and technology in a mortuary context at Atuel river valley: Cañada Seca-1 archaelogical record (San Rafael, Mendoza)

Este trabajo tiene como objetivo caracterizar el sitio Cañada Seca-1, un entierro múltiple (NMI=24) localizado en el valle del Atuel y fechado en ca. 1500 años AP, a través de la integración de diferentes líneas de evidencia. A partir de este registro se discuten las interpretaciones en torno a la presencia de cultígenos y sus implicancias para la subsistencia humana en un área caracterizada tradicionalmente como el límite de la dispersión agrícola sudamericana, ca. 2200 años AP. Los resultados destacan que los individuos inhumados en el sitio no habrían desarrollado una estrategia fundamentalmente agrícola, aunque el maíz formó parte de su dieta. Además, la nueva información sobre los comportamientos mortuorios en la zona del valle del Atuel, ofrece evidencias tanto sobre movilidad como sobre el uso de tecnologías utilitarias (líticas) y no utilitarias (ornamentos líticos y malacológicos) que trascendieron la vida cotidiana y los acompañaron en la muerte.The goal of this paper is to describe an archaeological burial site with commingled skeletal remains, named Cañada Seca-1 (MNI= 24), located in the Atuel River valley and dating from ca. 1500 years BP. This is performed by integrating well-known and recently developed lines of inquiry. Based on this record, we discuss interpretations related to cultigen presence and its implications in human subsistence and mobility, in an area traditionally characterized as the pre-Hispanic agricultural limit, ca. 2200 years BP. Results show that the individuals did not developed a farming strategy, although maize seems to have been part of their diet. In turn, the new information about mortuary behavior in the Atuel River valley provides new insights regarding mobility, as well as the use of utilitarian (lithics) and nonutilitarian (lithic and malacological ornaments) technology that went beyond these peoples’ life into their death.Sociedad Argentina de Antropologí

EcoCyc: a comprehensive database of Escherichia coli biology

EcoCyc (http://EcoCyc.org) is a comprehensive model organism database for Escherichia coli K-12 MG1655. From the scientific literature, EcoCyc captures the functions of individual E. coli gene products; their regulation at the transcriptional, post-transcriptional and protein level; and their organization into operons, complexes and pathways. EcoCyc users can search and browse the information in multiple ways. Recent improvements to the EcoCyc Web interface include combined gene/protein pages and a Regulation Summary Diagram displaying a graphical overview of all known regulatory inputs to gene expression and protein activity. The graphical representation of signal transduction pathways has been updated, and the cellular and regulatory overviews were enhanced with new functionality. A specialized undergraduate teaching resource using EcoCyc is being developed

Length of carotid stenosis predicts peri-procedural stroke or death and restenosis in patients randomized to endovascular treatment or endarterectomy.

BACKGROUND: The anatomy of carotid stenosis may influence the outcome of endovascular treatment or carotid endarterectomy. Whether anatomy favors one treatment over the other in terms of safety or efficacy has not been investigated in randomized trials. METHODS: In 414 patients with mostly symptomatic carotid stenosis randomized to endovascular treatment (angioplasty or stenting; n = 213) or carotid endarterectomy (n = 211) in the Carotid and Vertebral Artery Transluminal Angioplasty Study (CAVATAS), the degree and length of stenosis and plaque surface irregularity were assessed on baseline intraarterial angiography. Outcome measures were stroke or death occurring between randomization and 30 days after treatment, and ipsilateral stroke and restenosis ≥50% during follow-up. RESULTS: Carotid stenosis longer than 0.65 times the common carotid artery diameter was associated with increased risk of peri-procedural stroke or death after both endovascular treatment [odds ratio 2.79 (1.17-6.65), P = 0.02] and carotid endarterectomy [2.43 (1.03-5.73), P = 0.04], and with increased long-term risk of restenosis in endovascular treatment [hazard ratio 1.68 (1.12-2.53), P = 0.01]. The excess in restenosis after endovascular treatment compared with carotid endarterectomy was significantly greater in patients with long stenosis than with short stenosis at baseline (interaction P = 0.003). Results remained significant after multivariate adjustment. No associations were found for degree of stenosis and plaque surface. CONCLUSIONS: Increasing stenosis length is an independent risk factor for peri-procedural stroke or death in endovascular treatment and carotid endarterectomy, without favoring one treatment over the other. However, the excess restenosis rate after endovascular treatment compared with carotid endarterectomy increases with longer stenosis at baseline. Stenosis length merits further investigation in carotid revascularisation trials

Search for composite and exotic fermions at LEP 2

A search for unstable heavy fermions with the DELPHI detector at LEP is reported. Sequential and non-canonical leptons, as well as excited leptons and quarks, are considered. The data analysed correspond to an integrated luminosity of about 48 pb^{-1} at an e^+e^- centre-of-mass energy of 183 GeV and about 20 pb^{-1} equally shared between the centre-of-mass energies of 172 GeV and 161 GeV. The search for pair-produced new leptons establishes 95% confidence level mass limits in the region between 70 GeV/c^2 and 90 GeV/c^2, depending on the channel. The search for singly produced excited leptons and quarks establishes upper limits on the ratio of the coupling of the excited fermio

Search for charginos in e+e- interactions at sqrt(s) = 189 GeV

An update of the searches for charginos and gravitinos is presented, based on a data sample corresponding to the 158 pb^{-1} recorded by the DELPHI detector in 1998, at a centre-of-mass energy of 189 GeV. No evidence for a signal was found. The lower mass limits are 4-5 GeV/c^2 higher than those obtained at a centre-of-mass energy of 183 GeV. The (\mu,M_2) MSSM domain excluded by combining the chargino searches with neutralino searches at the Z resonance implies a limit on the mass of the lightest neutralino which, for a heavy sneutrino, is constrained to be above 31.0 GeV/c^2 for tan(beta) \geq 1.Comment: 22 pages, 8 figure

Hadronization properties of b quarks compared to light quarks in e+e- -> q qbar from 183 to 200 GeV

The DELPHI detector at LEP has collected 54 pb^{-1} of data at a centre-of-mass energy around 183 GeV during 1997, 158 pb^{-1} around 189 GeV during 1998, and 187 pb^{-1} between 192 and 200 GeV during 1999. These data were used to measure the average charged particle multiplicity in e+e- -> b bbar events, _{bb}, and the difference delta_{bl} between _{bb} and the multiplicity, _{ll}, in generic light quark (u,d,s) events: delta_{bl}(183 GeV) = 4.55 +/- 1.31 (stat) +/- 0.73 (syst) delta_{bl}(189 GeV) = 4.43 +/- 0.85 (stat) +/- 0.61 (syst) delta_{bl}(200 GeV) = 3.39 +/- 0.89 (stat) +/- 1.01 (syst). This result is consistent with QCD predictions, while it is inconsistent with calculations assuming that the multiplicity accompanying the decay of a heavy quark is independent of the mass of the quark itself.Comment: 13 pages, 2 figure