Orquiectomía en canino criptorquido unilateral

El presente trabajo corresponde a un caso quirúrgico de un paciente canino de 5 años de edad, que presentaba criptorquidismo. Se decidió la resolución quirúrgica, con el objetivo de evitar posibles complicaciones futuras. El procedimiento se realizó con éxito y el paciente se recuperó perfectamente. En pacientes criptórquidos, se recomienda realizar la esterilización a temprana edad

In vitro incubation of human spermatozoa promotes reactive oxygen species generation and DNA fragmentation

The aim of this study was to investigate the oxidative process associated with sperm capacitation and its impact on DNA fragmentation and sperm function. Redox activity and lipid peroxidation were analysed in human spermatozoa after 3, 6 and 22h of incubation in Hams F10 medium plus bovine albumin at 37 degrees and 5% CO2 for capacitation. DNA status, tyrosine phosphorylation pattern and induced acrosome reaction were evaluated after capacitating conditions. At 22h of incubation, there was a significant (P<0.05) increase in oxygen-free radicals and lipid peroxidation, with no effect on sperm viability. There also was a significant (P<0.001) increase in fragmented DNA in capacitated spermatozoa compared to semen values with higher rates being found after the occurrence of the induced acrosome reaction. Protein tyrosine phosphorylation pattern confirms that capacitation took place in parallel with the occurrence of DNA fragmentation. These results indicate that when spermatozoa are incubated for several hours (22h), a common practice in assisted reproductive techniques, an increase in oxidative sperm metabolism and in the proportion of fragmentedDNA should be expected. However, there was no effect on any of the other functional parameters associated with sperm fertilising capacity47886186

Effects of ulipristal acetate on sperm DNA fragmentation during in vitro incubation

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Objective Ulipristal acetate (UPA) acts as an emergency contraceptive by inhibiting ovulation. This study explores possible additional effects on the fragmentation of sperm DNA during in vitro incubation. Methods Motile spermatozoa from healthy donors were selected by swim-up and incubated under capacitating conditions in control medium or with UPA (1, 10, 100, 1,000 or 10,000 ng/ml). In some experiments, 200 mu M of H2O2 were added to induce oxidative stress. The sperm chromatin dispersion test was performed to analyse DNA integrity (400 cells; 1000X). Lipid peroxidation (thiobarbituric acid assay), induced-acrosome reaction (AR) and sperm vitality (Eosin Y) were also evaluated in spermatozoa exposed to UPA and/or H2O2. Results During sperm incubation, the percentage of fragmented DNA increased significantly, from 15.0 +/- 1.3 to 41.0 +/- 4.5% (p < 0.001). In the presence of UPA, DNA fragmentation decreased significantly (p < 0.05), in a dose-dependent manner. At 100 and 1000 ng/ml, UPA also counteracted the effect of H2O2 and prevented DNA fragmentation. No effect on sperm vitality, lipid peroxidation or induced-AR was found with any treatment. Conclusions During in vitro sperm capacitation DNA fragmentation increased but the latter was counteracted in the presence of UPA, which possibly acted as a scavenger of reactive oxygen species produced by spermatozoa.185355363Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)CNPq [573747/2008-3