Vaccination of rainbow trout against Streptococcus iniae infection: comparison of different routes of administration and different vaccines

Antibody production and clinical efficacy (relative percent survival RPS) were measured in 40±5g rainbow trout after immunization with two types of Streptococcus iniae vaccines consisting of formalin killed cells (FKC) and FKC enriched with the bacterial extracellular products (ECP) administered by intraperitoneal (i.p), immersion and oral routes at 16±1°C for 18 weeks. No significant difference was found in antibody levels among the fish i.p immunized with FKC enriched with ECP plus Freunds' adjuvant (FA), FKC plus FA and FKC vaccines (P>0.05), whilst the antibody production was significantly higher in these three groups than fish immunized by immersion and oral routes of FKC and FKC enriched ECP (P0.05). The RPSs ranging 82.6-100, 73.9-95 and 73.9-91.7% were obtained in the fish intraperitoneally immunized with FKC enriched ECP plus FA, FKC plus FA and FKC vaccines, respectively, compared to 0% survival for the control fish. Also, RPS in fish vaccinated by the immersion route was in the range 45.8-30.4% after 18 weeks post-vaccination. Efficacy of oral vaccination of fish with FKC plus ECP was in range of 8.7-29% and that of fish orally vaccinated with FKC resulted in 8.7-20.8% protection

Survey on health status in aquaculture sturgeons centers (Mazandaran, Guilan And Golestan Provinces)

Study of survey health management and diseases in hatcheries and fish farms can help us to knowledge and application control methods such as: prevention, treatment and increase high levels of production in hatchery and farms, finally. This survey carried out from 2005 to 2008 for 4 years in sturgeon hatcheries and farms of Golestan province. Sturgeon fishes include Huso Huso, Ship sturgeon, Acipenser persicus collected and for virology, bacteriology, fungius and hematology examined. Also, physicochemical parameters measured and recorded in different stages of culture. Results of this study showed that all of samples in virology was negative and did not observe any doubetful causes. In bacteriology CFU was variation from 3/9 ×105 to 6/9×10. The most parasites that detected in this survey was Cocolanus espherolanus, Sceria binopsulus semiarmatus and Amphilina fuliacea that separates from Acipenser Percicus, especially. The results about hematology parameters some important hematological indices of ship sturgeon include: The total RBC for female and mail specimens measured as 5.3±1.5 ×10^5, 4.8±0.5×10^5 per mm^3 respectively. The amount of haematocrit and hemoglobin for female and mail determined: 34.3±2.8, 35±1.4 percent and 10.3±0.9, 8.9±0.8 gr/dl .The MCV: 216.3± 96.2, 736.5± 102.5, MCH: 720.2±309.5, 186±0.7 and MCHC: 30±0.8, 25.5±3.4 percent respectively.The total WBC were (female, male): 21320±1054, 20580±777 per mm^3 and neutrophil: 16.4±2.5, 17±1.4 percent and lymphocyte: 74.4±2.4, 73.5± 0.7 percent and eosinophil: 6±1.4, 6.4±0.5 percent, monocyte: 2.8±0.8, 3.5±0.7 percent. There was not any significant differences (p>0.05) between mentioned parameters in male and female (students t-test). Also evaluation of hematological parameters in bluga ( Huso huso) include: total RBC were (male , female) 5±0.3 ×105 , 4.9±0.6 ×105 per mm^3 ,respectively and hematocrit: 33.2±6.7 , 35.4±3.4 percent and hemoglobin: 11.2±1.5 , 12.2±1gr/dl and MCV: 669.9±172.2, 723.9±982.4 and MCH: 226.2±42.5, 249.5±35.4 and MCHC: 34.1±2.4, 34.6±3.6 percent respectively. The totals WBC were (male, female): 24800±707.1, 23042±1375.4 per mm^3 and neutrophil: 18.5±0.7, 21.4±1.1 percent and lymphocyte: 73.5±1.4, 68.4±1.1 percent and eosinophil: 5±2.8, 7±1.2 percent and monocyte: 3.5±3.5, 3.2±0.8 percent. According to statistically study the count of lymphocyte had significant difference between male and female fish and this count in male was higher than female. (p≥0.05)

Study on viral nervouse necrosis (isolation, characterisation and pathogenesis) in golden grey mullet in the Caspian Sea and study of pathogenecity and possibility of transmission to the other fish species (Sturgeon fishes, Rutilus frisii kutum and reared Rainbow trout and Carp)

Study on Viral Nervouse necrosis (isolation, characterisation and pathogenesis) in Golden grey mullet in the Caspian Sea and study of pathogenecity and possibility of transmission to the other fish species (Sturgeon fishes, Rutilus frisii kutum and reared Rainbow trout and Carp) Viral Nervous Necrosis (VNN) is a worldwide disease affecting several species of cultured marine fish. For the past two decades, betanodavirus infections that cause Viral Nervous Necrosis (VNN) have emerged as major constraints on the culture and sea ranching of marine fish in almost all parts of the world. More than forty species mainly of marine origin have been so far affected and this number is likely to rise in future following the introduction of new species and the increase of aquaculture trade. Unknown acute mortality occurred in wild golden grey mullet Lisa auratus and Liza saliens in Iranian waters of Caspian Sea in recent years. In order to isolation and confirmation of causative agents of golden grey mullet mortality in the Caspian Sea, a complementary research investigation project was designed in 2005 and approved immediately in Iranian Fisheries Research Organization (IFRO). Many diagnostic aspects such as Virology (Cell culture and Elctereone Microscopy), Hemathology, Bacteriology, Histopathology, Molecular biology (NestedRT-PCR), Heavy metals measuerment and Serology (IFAT and IHC) were employed in mentioned multidiciplinary project. About 322 moribund fish samples which revealed skin darkening, erratic swimming behavior such as spiral and belly-up at rest and high distention of swimming bladder. Suspected samples were collected from coastal capture sites in iranian north proviences in 2006 till 2009. Targets tissue such as brain and eye were removed in strile condition and then kept in -80oC frezzer for cell culture and Nested-RT-PCR. Other tissue samples from liver, kidney, intestine, stomach, gill, skin and muscle, gall bladder and gonads were taken and fixed in 10% buffer formalin and same parts fixed in glutaraldehyde 3% for histopathology, IHC and EM respectively. Cytopathic effect (CPE) was observed in those cell cultures just six days after inoculation with the dilutions of the tested 312 homogenate supernatants. CPE in monolayers of cells cultured (SSN-1 cell line) was characterised by thin or rounded, refractile, granular cells with vacuoles. Nine samples were positive in virology assay. Nested- RT-PCR was done on suspected tissue samples and supernatant of CPE positive samples and 21 tissue samples and all CPE positive samples were positive. IFAT was selected as a confirmatory method for identifying viral strains replicating on cell cultures and carried out with rabbit anti-betanodavirus serum on suspected tissue samples and some smears of CPE positive samples. Some bright points approved betanodavirus antigen and confirmed cell culture and Nested-RT-PCR findings. In fixed tissue samples widespread and massive vacuolation were observed in brain, spinal cord, retina and optical nerve. In order to confirmation of diagnostic findings , IHC was done with monoclonal antibody antibetanodavirus and some red-brown points were observed. Theses findings revealed expected viral antigens and confirmed previous results. Moreover, virus particles with 25-30 nm in diameter were visualized in infected brain and retina using positive staining in TEM. Also pathogenicity test was employed to confirm the obtained results. So Guppy fish Poecilia reticulata and sturgeon fry were used instead of the experimental host due to ease of handling and susceptibility. After 15 days post infection, guppy bathed in VNN-infected tissue culture with 104 TCID50 showed clinical signs similar to naturally infected Golden grey mullet, and the mortality rate reached up to 100% in 75 dpi. When target organs were examined by cell culture isolation, serology, and histopathology, all revealed the presence of virus in the Guppy. Suspected supernatant injected to sturgeon fry through intravitreous injection and widespread vacuolation were observed in brain and spinal cord buy IHC and Real time PCR were negative. In conclusion, with attntion to obtained results in this investigation such as ecological factors, clinical signs, histopathological, virological and bacteriological results, molecular analysis, (IHC, IFAT, PCR), TEM demonstration, serological and hematological findings, it could be confirmed that VNNV was the main causative agent for disease outbreak in Golden grey mullet in Southern coastline of Caspian Sea

Laparoscopy in management of appendicitis in high-, middle-, and low-income countries: a multicenter, prospective, cohort study.

BACKGROUND: Appendicitis is the most common abdominal surgical emergency worldwide. Differences between high- and low-income settings in the availability of laparoscopic appendectomy, alternative management choices, and outcomes are poorly described. The aim was to identify variation in surgical management and outcomes of appendicitis within low-, middle-, and high-Human Development Index (HDI) countries worldwide. METHODS: This is a multicenter, international prospective cohort study. Consecutive sampling of patients undergoing emergency appendectomy over 6 months was conducted. Follow-up lasted 30 days. RESULTS: 4546 patients from 52 countries underwent appendectomy (2499 high-, 1540 middle-, and 507 low-HDI groups). Surgical site infection (SSI) rates were higher in low-HDI (OR 2.57, 95% CI 1.33-4.99, p = 0.005) but not middle-HDI countries (OR 1.38, 95% CI 0.76-2.52, p = 0.291), compared with high-HDI countries after adjustment. A laparoscopic approach was common in high-HDI countries (1693/2499, 67.7%), but infrequent in low-HDI (41/507, 8.1%) and middle-HDI (132/1540, 8.6%) groups. After accounting for case-mix, laparoscopy was still associated with fewer overall complications (OR 0.55, 95% CI 0.42-0.71, p < 0.001) and SSIs (OR 0.22, 95% CI 0.14-0.33, p < 0.001). In propensity-score matched groups within low-/middle-HDI countries, laparoscopy was still associated with fewer overall complications (OR 0.23 95% CI 0.11-0.44) and SSI (OR 0.21 95% CI 0.09-0.45). CONCLUSION: A laparoscopic approach is associated with better outcomes and availability appears to differ by country HDI. Despite the profound clinical, operational, and financial barriers to its widespread introduction, laparoscopy could significantly improve outcomes for patients in low-resource environments. TRIAL REGISTRATION: NCT02179112

Pooled analysis of WHO Surgical Safety Checklist use and mortality after emergency laparotomy

Background The World Health Organization (WHO) Surgical Safety Checklist has fostered safe practice for 10 years, yet its place in emergency surgery has not been assessed on a global scale. The aim of this study was to evaluate reported checklist use in emergency settings and examine the relationship with perioperative mortality in patients who had emergency laparotomy. Methods In two multinational cohort studies, adults undergoing emergency laparotomy were compared with those having elective gastrointestinal surgery. Relationships between reported checklist use and mortality were determined using multivariable logistic regression and bootstrapped simulation. Results Of 12 296 patients included from 76 countries, 4843 underwent emergency laparotomy. After adjusting for patient and disease factors, checklist use before emergency laparotomy was more common in countries with a high Human Development Index (HDI) (2455 of 2741, 89.6 per cent) compared with that in countries with a middle (753 of 1242, 60.6 per cent; odds ratio (OR) 0.17, 95 per cent c.i. 0.14 to 0.21, P <0001) or low (363 of 860, 422 per cent; OR 008, 007 to 010, P <0.001) HDI. Checklist use was less common in elective surgery than for emergency laparotomy in high-HDI countries (risk difference -94 (95 per cent c.i. -11.9 to -6.9) per cent; P <0001), but the relationship was reversed in low-HDI countries (+121 (+7.0 to +173) per cent; P <0001). In multivariable models, checklist use was associated with a lower 30-day perioperative mortality (OR 0.60, 0.50 to 073; P <0.001). The greatest absolute benefit was seen for emergency surgery in low- and middle-HDI countries. Conclusion Checklist use in emergency laparotomy was associated with a significantly lower perioperative mortality rate. Checklist use in low-HDI countries was half that in high-HDI countries.Peer reviewe

Global variation in anastomosis and end colostomy formation following left-sided colorectal resection

Background End colostomy rates following colorectal resection vary across institutions in high-income settings, being influenced by patient, disease, surgeon and system factors. This study aimed to assess global variation in end colostomy rates after left-sided colorectal resection. Methods This study comprised an analysis of GlobalSurg-1 and -2 international, prospective, observational cohort studies (2014, 2016), including consecutive adult patients undergoing elective or emergency left-sided colorectal resection within discrete 2-week windows. Countries were grouped into high-, middle- and low-income tertiles according to the United Nations Human Development Index (HDI). Factors associated with colostomy formation versus primary anastomosis were explored using a multilevel, multivariable logistic regression model. Results In total, 1635 patients from 242 hospitals in 57 countries undergoing left-sided colorectal resection were included: 113 (6·9 per cent) from low-HDI, 254 (15·5 per cent) from middle-HDI and 1268 (77·6 per cent) from high-HDI countries. There was a higher proportion of patients with perforated disease (57·5, 40·9 and 35·4 per cent; P < 0·001) and subsequent use of end colostomy (52·2, 24·8 and 18·9 per cent; P < 0·001) in low- compared with middle- and high-HDI settings. The association with colostomy use in low-HDI settings persisted (odds ratio (OR) 3·20, 95 per cent c.i. 1·35 to 7·57; P = 0·008) after risk adjustment for malignant disease (OR 2·34, 1·65 to 3·32; P < 0·001), emergency surgery (OR 4·08, 2·73 to 6·10; P < 0·001), time to operation at least 48 h (OR 1·99, 1·28 to 3·09; P = 0·002) and disease perforation (OR 4·00, 2·81 to 5·69; P < 0·001). Conclusion Global differences existed in the proportion of patients receiving end stomas after left-sided colorectal resection based on income, which went beyond case mix alone

Title:Evaluation of Optimal Water Allocation Scenarios for Bar River of NeishabourUsing WEAP Model Under A2 Climatic Changes Scenario

Introduction: The rapid population growth in Iran and the corresponding increases in water demands, including drinking water, industry, agriculture and urban development and existing constraints necessitate optimal scheduling necessity in use of this crucial source. Furthermore, the phenomenon of climate change as a major challenge for humanity can be considered in future periods. Climate change is caused by human activity have also been identified as significant causes of recent climate change, referred to as "global warming". Climate change indicates an unusual change in the Earth's atmosphere and climate consequences of the different parts of planet Earth. Climate change may refer to a change in average weather conditions, or in the time variation of weather around longer-term average conditions. A Warmer climate exacerbates the hydrologic cycle, altering precipitation, magnitude and timing of runoff. The purpose of this study was to evaluate the effect of climate change on water consumption and demand in Bar river basin of Neighbor. Climate change affects precipitation and temperature patterns and hence, may alter on water requirements and demand at three sectors; agriculture, industry and urban water. Materials and Methods: At present, Global coupled atmosphere-ocean general circulation models (AOGCMs) are the most frequently used models for projection of different climatic change scenarios. AOGCMs models represent the pinnacle of complexity in climate models and internalize as many processes as possible. These models are based on physical laws that are provided by mathematical relations. AOGCMs models used for climate studies and climate forecast are run at coarse spatial resolution and are unable to resolve important sub-grid scale features such as clouds and topography. As a result AOGCMs output cannot be used for local impact studies. Therefore, downscaling methods were developed to obtain local-scale weather and climate, particularly at the surface level, from regional-scale atmospheric variables that are provided by AOGCMs. Four different downscaling methods exist: regression methods, weather pattern-based approaches, stochastic weather generators, which are all statistical downscaling methods, and limited-area modeling. For this research, HadCM3 and statistical downscaling model (SDSM), precipitation and temperature variations were simulated under A2 scenario. Then the impacts of these variations on Bar River discharge were analyzed, i.e. water resources at three sectors of agriculture, industrial and potable water under climate change during 2011-2040 using WEAP. Results at first part of simulation showed that temperature is increasing and precipitation is decreasing resulted in decreasing of Bar discharge. According to the decreasing on Bar discharge, water allocation was simulated under these conditions of agricultural and industrial development and increasing of population with WEAP. Simulation showed that watershed will face increasing of water demand for all three sectors; agriculture, industry and drinking water, so the highest water shortage would be in agricultural demand and then industry and drinking water respectively. IWRM is the basic managerial need to rest the demands especially for drought periods. Current allocation process is based on steady state conditions while allocation pattern would be done under climate change conditions so we need to be reinvestigat the last allocations for all three sectors. Another challenge for this watershed refers to the gardens and steel factory of Khorasan that they need to use new technologies for reduction of their water needs. Results Discussion: In this study, the outputs of General Circulation Models (HadCM3) and statistical downscaling model (SDSM) have been used to investigate the changes of rainfall and temperature under A2 scenario in Bar river basin of Neishaboor and assess the impacts of this changes on the Bar river’s discharge. Finally, using WEAP model under climate change conditions for the period of 2011-2040, the status of basin water resources was evaluated for the three sectors (agricultural, domestic and industrial). The results indicated increased temperature in the Arie station amounting to 16 percent and rainfall reduction in the Arie and Taghan stations amounting to 3.9 and 8.75 percent respectively. Under these conditions, according to the increasing water demands of agricultural and industrial sectors in the future, there will be a shortage of water supply resources in the region. So the agricultural sector with 12 percent will have the highest percentage of water shortage and water scarcity and of the industrial sector will be 2%. However, the drinking water or domestic demand will not face a shortage of supplies. Conclusion: Therefore given that the most part of agriculture sector’s share of basin is allocated to orchards and on the other hand the most shortages are related to agriculture, then while creating an integrated management of water resources, development and use of modern methods of irrigation during the period of 2011 - 2040 would seem to be necessary

Investigating Prevalence of deaths from Traffic Accidents and Factors Associated with it in Yazd in 2009

Introduction: Traffic accidents are regarded as the most common cause of injuries and in fact, as the second leading cause of death in the country. Furthermore, the highest number of deaths from traffic accidents in the world belonged to the majority of young people aged 20 to 30 years. Therefore, the aim of this study was to determine the prevalence of deaths from traffic accidents and factors associated with it in Yazd in 2009. Methods: This was a cross-sectional descriptive and analytical study. The study participants involved the victims of traffic accidents whose injuries were recorded by the authorities in 2009 in Yazd. According to statistical calculations, 2000 events leading to injury were selected using a random table. Then, a questionnaire was completed accordingly. The study data were analyzed using the statistical tests such as T-test ,Chi-square, and logistic regression via SPSS software (version 16). Results: Within 2,000 victims of traffic accidents, 94.9 % involved the males and 5.1 % were females among whom 3.4 % died. Most victims aged 21 to 35 years (54.2 %). The highest percentage of deaths were related to excessive speed (5.6 %) and driving in the opposite direction (4.8 %) (P= 0.01). Also, the highest percentage of deaths were related to the motorcyclists (34 cases) that none had no helmets . Conclusion: This study findings provide useful information for setting priorities in order to prevent the traffic accidents injuries. In addition, appropriate intervention programs are necessary in order to prevent traffic accidents and their complications, as well as to minimize injuries in accordance with other relevant organizations