Internet gaming disorder and problematic internet use: the links between cyber-bullying, aggression, loneliness and psychiatric comorbidities

Aims: To compare adolescents clinically diagnosed with Internet Gaming Disorder (IGD) and problematic internet use (PIU) in terms of cyberbullying, aggression, and loneliness. Methods: Male adolescent patients (N=124, 14.3±1.7 years) with Internet Addiction Scale (IAS) scores ≥50 were clinically interviewed for IGD in utilizing DSM-5 criteria. Patients without full IGD criteria were included as PIU comparisons. Clinical variables were assessed using the second version of the Revised Cyber Bullying Inventory, short-form of the UCLA Loneliness Scale, Buss Perry Aggression Questionnaire, Child Depression Inventory, and Screen for Child Anxiety Related Emotional Disorders. Results: Compared to individuals with PIU, those with IGD were significantly more likely to have attention-deficit hyperactivity disorder, higher social phobia scores, higher cyberbullying scores, higher loneliness scores, been a cyberbully, and been a cyberbully victim. Conclusion: Male adolescents with IGD have higher rates of psychiatric comorbidity, perceived loneliness, cyberbullying, and being a victim of cyberbullying than those with PIU. Future studies could evaluate these predictors of transition from PIU to IGD in large cohort samples

Global patient outcomes after elective surgery: prospective cohort study in 27 low-, middle- and high-income countries.

BACKGROUND: As global initiatives increase patient access to surgical treatments, there remains a need to understand the adverse effects of surgery and define appropriate levels of perioperative care. METHODS: We designed a prospective international 7-day cohort study of outcomes following elective adult inpatient surgery in 27 countries. The primary outcome was in-hospital complications. Secondary outcomes were death following a complication (failure to rescue) and death in hospital. Process measures were admission to critical care immediately after surgery or to treat a complication and duration of hospital stay. A single definition of critical care was used for all countries. RESULTS: A total of 474 hospitals in 19 high-, 7 middle- and 1 low-income country were included in the primary analysis. Data included 44 814 patients with a median hospital stay of 4 (range 2-7) days. A total of 7508 patients (16.8%) developed one or more postoperative complication and 207 died (0.5%). The overall mortality among patients who developed complications was 2.8%. Mortality following complications ranged from 2.4% for pulmonary embolism to 43.9% for cardiac arrest. A total of 4360 (9.7%) patients were admitted to a critical care unit as routine immediately after surgery, of whom 2198 (50.4%) developed a complication, with 105 (2.4%) deaths. A total of 1233 patients (16.4%) were admitted to a critical care unit to treat complications, with 119 (9.7%) deaths. Despite lower baseline risk, outcomes were similar in low- and middle-income compared with high-income countries. CONCLUSIONS: Poor patient outcomes are common after inpatient surgery. Global initiatives to increase access to surgical treatments should also address the need for safe perioperative care. STUDY REGISTRATION: ISRCTN5181700

Biosensor immunoassay for flumequine in broiler serum and muscle

Flumequine (Flu) is one of the fluoroquinolones most frequently applied for the treatment of broilers in The Netherlands. For the detection of residues of Flu in blood serum of broilers, a biosensor immunoassay (BIA) was developed which was fast (7.5 min per sample) and specific (no cross-reactivity with other (fluoro)quinolones). This inhibition assay was based on a rabbit polyclonal anti-Flu serum and a CM5 biosensor chip coated with Flu which could be detected in the range of 15-800 ng mL(-1). For the detection of Flu in muscle, an easy extraction procedure in buffer was selected and the measuring range was from 24 to 4000 ng g(-1). Average recoveries of 66 till 75% were found with muscle samples spiked at 0.5, 1 and 2 times the maximum residue limit (MRL in muscle = 400 ng g(-1)) and the decision limit (CC alpha) and the detection capability (CC beta) were determined as 500 and 600 ng respectively. Incurred muscle samples were analysed by the BIA and by LC-MS/MS and a good correlation was found (R-2 = 0.998). Serum and muscle samples from with Flu treated broilers were analysed and the concentrations found in serum were always higher than those found in muscle (average serum/muscle ratio was 3.5) and this proved the applicability of the BIA in serum as predictor of the Flu concentration in muscle. (c) 2006 Elsevier B.V. All rights reserved

Utilization of shellfish waste: Effects of Chitosan from shrimp shell waste on fatty acid profiles of European eel

The effects of chitosan extracted from shrimp (Metapenaeus stebbingi) shell waste and commercial chitosan on fatty acid profile of European eel (Anguilla anguilla) fillet pieces during refrigerated storage were investigated. Fish pieces samples were treated with aqueous solution of 0.1 and 1% extracted and commercial chitosan, and then stored at 4°C for 18 days. The fillet pieces samples were analyzed periodically for fatty acid analysis. No statistically significant change was detected in the total saturated fatty acid (SFA) contents of eel fillet pieces in the all groups at the end of storage (p>0.05). There were no significant difference in monounsaturated fatty acid (MUFA) and polyunsaturated fatty acid (PUFA) contents of the groups added 1% extracted and commercial chitosan (p>0.05), while a percentage decrease was determined in MUFA and PUFA contents of groups control, added 0.1% extracted and commercial chitosan at the end of storage (p<0.05). It was concluded appropriate to use chitosan obtained from shrimp shells not evaluated and discarded as waste products in Turkey during the storage of fatty fish like eel.This work was supported by Scientific Research Fund of Cukurova University in Turkey