Characterization of the translational landscape of the plant-virus interaction

Tomato yellow leaf curl virus (TYLCV) is responsible for a disease that causes massive damage to tomato crops around the world (Prasad et al., 2020). Due to its viral nature, it requires the host’s cellular machinery to be able to infect, which implies complex interactions between the virus and the plant. Most studies about this association are based on transcriptomics and interactomics, while translatomics analyses have, so far, been scarce. Understanding the translational machinery that is responsible for the production of viral proteins and, consequently, its propagation will allow to shed some light on these interactions and gain knowledge about the changes at the translational level that tomato plants experience upon infection. To that end, we are characterizing the translational landscape of the plant-virus interaction using the emerging technique Ribo-Seq. And to deepen our knowledge on the regulatory mechanisms involved in the translational response, two isogenic tomato lines, one resistant (the ty-5 mutant) and one susceptible (Santa Clara) to TYLCV are being employed. Ty-5 is a recessive mutation located on the Pelota gene, which is involved in the recycling phase of the translation cycle (Lapidot et al., 2015), so the study of this mutant will inform about the role of the translational machinery in the viral infection. In addition, and using RIP+MS, we are attempting to uncover the translational machinery associated to viral transcripts to determine if certain riboproteins or translation factors are preferred for the translation of viral transcripts. We will present the advances we have made regarding these objectives.Acknowlegments: The authors are grateful to Rafael Fernández-Muñoz (IHSM) for sharing the ty-5 and Santa Clara tomato seeds. This work is funded by Grant P18-RT-1218 from the Junta de Andalucía to CM and ERB, and a RYC- 2017-1218 to CM. The authors also thank the Plan Propio de Ingestigación of the University of Málaga, Campus de Excelencia Andalucía Tech

The citizens’ perspective : awareness, feelings and acceptance of surveillance and surveillance systems for fighting crime in Spain. A quantitative study

This document presents the results for Spain within the framework of a larger study undertaken as part of the RESPECT project – “Rules, Expectations and Security through Privacy-enhanced Convenient Technologies” (RESPECT; G.A. 285582) – which was co-financed by the European Commission within the Seventh Framework Programme (2007-2013). Analyses are based on a survey regarding the perceptions, feelings, attitudes and behaviours of citizens towards surveillance for the purpose of fighting crime, carried out amongst a quota sample that is representative of the population in Spain for age and gender. Responses were gathered, predominantly, through an online survey supplemented by a number of questionnaires administered in face to face interviews, in order to fulfil the quota and also reach those citizens who do not use the internet. The questionnaire consisted of 50 questions and was available online in all languages of the European Union between November 2013 and March 2014. The face to face interviews were carried out between January and March 2014. The Spanish sample is based on the responses from 500 individuals who indicated Spain as their country of residence in the online survey or were administered the questionnaire face to face. As a result, the Spanish respondents indicated a strongly felt lack of trust in the protection of, and control over, personal information gathered via surveillance. At the same time, and despite the respondents’ general perception of surveillance measures being useful, surveillance measures appear not to reduce their feelings of insecurity, and analyses indicate that increasing the effectiveness of surveillance measures may not increase citizens’ feelings of security at the same rate. However, results also point at the possibility that increasing the effectiveness of laws regarding the protection of personal data gathered via surveillance may make citizens feel more secure. Further, the majority of Spanish respondents feel more unhappy than happy with the different types of surveillance (except CCTV), and particularly unhappy about surveillance taking place without them knowing about it. However, there is only a moderate link between feeling happy, or unhappy, about surveillance and feeling secure or insecure through the presence of surveillance. More research is needed to disentangle the relationships and effects between surveillance measures, feelings of security or insecurity, and citizens’ general quality of life feelings.This project has received funding from the European Union’s Seventh Framework Programme for research, technological development and demonstration under grant agreement no 285582.peer-reviewe

Evaluation of mass transfer coefficients in biotrickling filters: experimental determination and comparison to correlations

This is the pre-peer reviewed version of the following article: Dorado, A.D. [et al.]. Evaluation of mass transfer coefficients in biotrickling filters: experimental determination and comparison to correlations. "Chemical engineering and technology", Setembre 2009, vol. 32, núm. 12, p. 1941-1950, which has been published in final form at https://doi.org/10.1002/ceat.200900275. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.Overall mass transfer coefficients (KGa and KLa) were determined experimentally for four different-nature packing materials used in gas-phase biotrickling filters. A simple methodology based on overall mass balances and following a standard procedure allowed to calculate the mass transfer coefficients under different operating conditions corresponding to usual biotrickling filtration situations. Results showed an increase of mass transfer resistance when increasing the empty bed residence time (EBRT) of the reactor for all packing materials. Experimental results were fitted to existing and well-accepted correlations used in conventional biofilter or biotrickling filter modeling. The comparison of experimental and theoretical data showed huge discrepancies. Simple correlations for the experimental data obtained in this study were also suggested.Peer ReviewedPostprint (author's final draft

Characterization of the translational landscape of the plant-virus interaction

Tomato yellow leaf curl virus (TYLCV) is responsible for a disease that causes massive damage to tomato crops around the world (Prasad et al., 2020). Due to its viral nature, it requires the host’s cellular machinery to be able to infect, which implies complex interactions between the virus and the plant. Most studies about this association are based on transcriptomics and interactomics, while translatomics analyses have, so far, been scarce. Understanding the translational machinery that is responsible for the production of viral proteins and, consequently, its propagation will allow to shed some light on these interactions and gain knowledge about the changes at the translational level that tomato plants experience upon infection. To that end, we are characterizing the translational landscape of the plant-virus interaction using the emerging technique Ribo-Seq. And to deepen our knowledge on the regulatory mechanisms involved in the translational response, two isogenic tomato lines, one resistant (the ty-5 mutant) and one susceptible (Santa Clara) to TYLCV are being employed. Ty-5 is a recessive mutation located on the Pelota gene, which is involved in the recycling phase of the translation cycle (Lapidot et al. 2015), so the study of this mutant will inform about the role of the translational machinery in the viral infection. In addition, and using RIP+MS, we are attempting to uncover the translational machinery associated to viral transcripts to determine if certain riboproteins or translation factors are preferred for the translation of viral transcripts. We will present the advances we have made regarding these objectives.The authors are grateful to Rafael Fernández-Muñoz (IHSM) for sharing the ty-5 and Santa Clara tomato seeds. This work is funded by Grant P18-RT-1218 from the Junta de Andalucía to CM and ERB, a RYC-2017-1218 to CM and the “Plan Propio” from the Universidad de Málaga. Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tec

Informe Jurídico sobre la Resolución Directoral N° 429-2021-JUS/DGTAIPD-DPDP

Para la regulación del tratamiento de los datos sensibles médicos de trabajadores existen diversos deberes y derechos fundamentales involucrados: el derecho fundamental a la seguridad y salud en el trabajo, el deber de vigilancia de la salud de los trabajadores y no discriminación en el trabajo, el derecho a la protección de información sensible médica, el deber de respeto y confidencialidad de la información íntima del trabajador y el derecho y deber al secreto profesional médico. Estos deberes y derechos se encuentran comprendidos en tres fuentes normativas que deben interpretarse en consonancia: Las normas de seguridad y salud en el trabajo, las normas de protección de datos personales y las normas de salud. En el caso concreto se analiza si resulta válido que el empleador acceda, efectúe el tratamiento y transmita a la SUNAFIL los datos sensibles médicos del trabajador denunciante sin contar con un consentimiento informado, conforme a las garantías que otorgan los artículos 13.6 y 18 de la Ley de Protección de Datos Personales, interpretadas en el marco de la asimetría propia de una relación laboral.For the regulation of the treatment of sensitive medical data of workers, there are various duties and fundamental rights involved: the fundamental right to safety and health at work, the duty to monitor the health of workers and non-discrimination at work, the right to the protection of sensitive medical information, the duty of respect and confidentiality of the intimate information of the worker and the right and duty to medical professional secrecy. These duties and rights are included in three normative sources that must be interpreted in consonance: Safety and health regulations at work, personal data protection regulations and health regulations. In the specific case, it is analyzed whether it is valid for the employer to access, carry out the treatment and transmit to SUNAFIL the sensitive medical data of the complainant worker without having informed consent, in accordance with the guarantees granted by articles 13.6 and 18 of the Law. Protection of Personal Data, interpreted within the framework of the asymmetry of an employment relationship

Improved Alveolar Dynamics and Structure After Alveolar Epithelial Type II Cell Transplantation in Bleomycin Induced Lung Fibrosis

Idiopathic pulmonary fibrosis (IPF) is a progressively and ultimately fatal lung disease. Previously it has been shown that intratracheal administration of alveolar epithelial type II cells (AE2C) in the animal model of bleomycin-induced pulmonary fibrosis is able to reverse fibrosis and restore surfactant protein levels. However, to date, it has not been evaluated whether these changes involve any improvement in alveolar dynamics. Consequently, the aim of the present work was to study lung physiology after AE2C transplantation at different time points during the development of injury and fibrosis. Lung fibrosis was induced by intratracheal instillation of bleomycin (4U/kg) in rat lungs. The animals were transplanted with AE2C (2.5 x 10(6) cells/animal) 3 or 7 days after bleomycin instillation. Assessments were done at day 7 and 14 after the induction of fibrosis to plot time dependent changes in lung physiology and mechanics. To assess the pressures and rates at which closed alveoli reopens invasive pulmonary tests using a small-animal mechanical ventilator (Flexivent (R), Scireq, Canada) including de-recruitability tests and forced oscillation technique as well as quasi-static pressure volume loops were performed. Afterwards lungs were fixed by vascular perfusion and subjected to design-based stereological evaluation at light and electron microscopy level. AE2C delivered during the lung injury phase (3 days) of the disease are only able to slightly recover the volume of AE2C and volume fraction of LB in AE2C. However, it did not show either positive effects regarding ventilated alveolar surface nor any increase of lung compliance. On the other hand, when AE2C are delivered at the beginning of the fibrotic phase (7 days after bleomycin instillation), an increased ventilated alveolar surface to control levels and reduced septal wall thickness can be observed. Moreover, transplanted animals showed better lung performance, with increased inspiratory capacity and compliance. In addition, a detailed analysis of surfactant active forms [mainly tubular myelin, lamellar body (LB)-like structures and multilamellar vesicles (MLV)], showed an effective recovery during the pro-fibrotic phase due to the healthy AE2C transplantation. In conclusion, AE2C transplantation during fibrogenic phases of the disease improves lung performance, structure and surfactant ultrastructure in bleomycin-induced lung fibrosis

Self-compacting concrete in the Temple of Sagrada Familia

The Temple of Sagrada Familia presents singularities in terms of both the original design by architect Antoni Gaudi and the duration of the construction, which started more than 120 years ago. In fact, its design was conceived before the development of reinforced concrete. Therefore, the construction materials and processes have evolved to adapt new technologies without compromising the vision of Gaudi. This willingness to maintain the original designs has obliged the technicians to resolve details that were not defined in the project, including the issue of how to cast elements at great heights and with a high amount of reinforcement. In this context, the possibility of using self-compacting concrete (SCC) emerged. This paper describes the different experiences in the Temple of Sagrada Familia with SCC, from requirements that led to using this material to the design of the mixes and the casting of the elements in situ

Serotonin re-uptake transporter gene polymorphisms are associated with imatinib-induced diarrhoea in chronic myeloid leukaemia patients.

Tyrosine kinase inhibitors (TKIs), the treatment of choice for chronic myeloid leukaemia (CML), can cause lower gastrointestinal (GI) toxicity which is manifested as diarrhoea. The mechanisms are not fully understood. The enteroendocrine signalling compound, serotonin (5-HT), is important for regulating peristaltic motion, fluid secretion and visceral hypersensitivity in the GI tract, and has been implicated in diseases such as irritable bowel syndrome. In this study, we have evaluated whether TKI-induced diarrhoea may be related to variation in the serotonin re-uptake transporter (SERT) gene. CML patients with and without diarrhoea on the SPIRIT2 trial (imatinib, n = 319; and dasatinib, n = 297) were genotyped for the promoter 5-HTTLPR, intron 2 VNTR and rs25531 polymorphisms by PCR-based methods. Diarrhoea was more prevalent in imatinib, than in dasatinib treated patients (P = 0.015), which when stratified by gender was seen to be driven by female patients (P = 0.036). Logistic regression analysis revealed that age, and the dominant HTTLPR with the rs25531 single nucleotide polymorphism (SNP) model, explained the occurrence of diarrhoea in ~10% of imatinib-treated female CML patients. These data suggest SERT polymorphisms influence imatinib-induced diarrhoea but not that of dasatinib

An exploratory study comparing psychological profiles and its congruence with clinical performance among patients with functional or motility digestive disorders

Functional gastrointestinal disorders have been related with different psychological conditions. On the contrary, the role of psychological factors within gastrointestinal motor disorders remains unclear. The objective of this study was to explore the differences and congruence with clinical performance of the psychological profile and subjective functionality among patients diagnosed with functional gastrointestinal disorders and gastrointestinal motor disorders. Using a double-blind design, 56 inpatients from a Gastroenterology Department were included in the study. No major differences were detected between the two groups. However, clinical performance was coherent with subjective physical functioning only among patients diagnosed with gastrointestinal motor disorders. These results may provide useful information for gastroenterologists dealing with patients' complaints not consistent with their clinical profile