Qualité biologique de l’eau de mer évaluée in situ par le test embryo-larvaire de Crassostrea gigas et Mytilus galloprovincialis = In situ monitoring of sea water quality with the embryo-larval bioassay of Crassostrea gigas and Mytilus galloprovincialis

Embryos and larvae of bivalves are frequently used in marine ecotoxicology for the purpose of assessing seawater quality, because they are very sensitive to pollutants and provide rapid responses. Laboratory studies, however, cannot accurately simulate natural conditions. We conducted bivalve embryo-larval studies in situ at the marina of Arcachon (southwest French Atlantic coast), in order to assess ‘biological quality’ of the water. One experiment conducted in winter 1999 (temperatures of 10 °C) with embryos of the Mediterranean mussel, Mytilus galloprovincialis, has shown that such tests are practicable in winter at low temperatures. This study did not show any deterioration in ‘biological quality’ of the water. Four series of experiments were subsequently performed during summer 2000 (ambient water temperatures of 19 to 22.4 °C) with embryos of the Japanese oyster, Crassostrea gigas. The results show that the ‘sea water biological quality’ deteriorates from the port entrance towards its inner part. To our knowledge, this is the first investigation of the marine environment in which bivalve embryos have been used in situ. They are very suitable for this type of study, because bivalve embryos and larvae are more sensitive to pollutants than the adults, and also because they belong to euryhaline species and the embryos tolerate summer temperatures (both species) as well as winter temperatures (mussels), allowing biomonitoring to be conducted all over the year

Modeling of the plasma jet of a stationary plasma thruster

We have developed a two-dimensional hybrid fluid – particle-in-cell Monte Carlo collisions (PIC- MCC) model to study the plume of a stationary plasma thruster. The model is based on a fluid description of the electrons (the electron density follows a Boltzmann distribution) and a particle description of the ion and neutral transport. Collisions between heavy species are taken into account with a Monte Carlo method. The electric field is obtained from Poisson's equation or from the quasineutrality assumption. We first show that the results from the PIC-MCC model are close to the results of a more time-consuming direct simulation Monte Carlo approach. We then compare the model predictions of the plume density and ion energy distribution with experimental measurements. Finally, we present a brief discussion on the assumptions of the model and on its ability to give reliable predictions on important issues such as the flux of ions backscattered to the satellite. © 2002 American Institute of Physics.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/70320/2/JAPIAU-91-12-9521-1.pd

Analysis of TerraSAR-X data and their sensitivity to soil surface parameters over bare agricultural fields

International audienceCette recherche a pour objectif de mettre en évidence la contribution des capteurs haute résolution pour une meilleure caractérisation de la surface du sol et pour analyser les effets de la polarisation et de l'angle d'incidence radar. L'objectif de cet article est d'analyser la sensibilité des données haute résolution Terrassar-X sur des sols sans couverture végétale et d'étudier la variabilité spatiale. Les relations entre le coefficient de rétrodiffusion et les paramètres du sol seront étudiés grâce aux images (TerraSAR, Ikonos, SPOT) ainsi que grâce aux mesures de terrain recueillies pendant plusieurs campagnes de terrain en hiver et printemps 2008-2009. Ce travail a été mené sur le bassin de l'Orgeval (France). / Our research aims to show the contribution of high resolution spatial sensors for a better characterization of soil surface, and to analyze polarization effects and radar incidence angle. The objective of this paper is to analyze the sensitivity of very high resolution TerraSAR-X radar data taken over bare soils, and to study the spatial variability. The relationship between backscattering coefficient and soil's parameters (moisture, surface roughness, and texture) will be examined by means of satellite images (TerraSAR, Ikonos, SPOT), as well as ground truth measurements, recorded during several field campaigns in the winter and spring of 2008 and 2009. This study is carried out on Orgeval catchment (France)

CRISPR/Cas9 facilitates rapid generation of constitutive forms of transcription factors in Aspergillus niger through specific on-site genomic mutations resulting in increased saccharification of plant biomass

The CRISPR/Cas9 system has been successfully applied for gene editing in filamentous fungi. Previous studies reported that single stranded oligonucleotides can be used as repair templates to induce point mutations in some filamentous fungi belonging to genus Aspergillus. In Aspergillus niger, extensive research has been performed on regulation of plant biomass degradation, addressing transcription factors such as XlnR or GaaR, involved in (hemi-)cellulose and pectin utilization, respectively. Single nucleotide mutations leading to constitutively active forms of XlnR and GaaR have been previously reported. However, the mutations were performed by the introduction of versions obtained through site-directed or UV-mutagenesis into the genome. Here we report a more time- and cost-efficient approach to obtaining constitutively active versions by application of the CRISPR/Cas9 system to generate the desired mutation on-site in the A. niger genome. This was also achieved using only 60-mer single stranded oligonucleotides, shorter than the previously reported 90-mer strands. In this study, we show that CRISPR/Cas9 can also be used to efficiently change functional properties of the proteins encoded by the target gene by on-site genomic mutations in A. niger. The obtained strains with constitutively active XlnR and GaaR versions resulted in increased production of plant biomass degrading enzymes and improved release of D-xylose and L-arabinose from wheat bran, and D-galacturonic acid from sugar beet pulp.Peer reviewe

One Dimensional Hybrid-Vlasov Simulation of a Hall Thruster

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/97103/1/AIAA2012-4313.pd

Practical formulas for the refraction coefficient

Knowledge of the actual refraction coefficient is essential in leveling surveys and precise electromagnetic distance measurement reduction. The most common method followed by the surveyor for its determination is based on the use of simultaneous reciprocal zenith observations. The commonly used formula is only an approximation valid for approximately horizontal sightings, whereas the exact geometric solution turns out to be very complicated so that an iterative computation procedure is suggested instead. In the present paper, the goal is to derive a compact formula from the complete solution that is easy to implement and retains the necessary accuracy for horizontal and slanted sightings. In addition, the paper will also focus on the common situation for the surveyor where isolated observations have to be done and no partially compensating procedures—e.g., leap-frog or middle point—are possible. If temperature vertical profiles are unknown then the refraction coefficient cannot be reliably determined. Some surveyors may customarily use then an average value, e.g., k 5 0:13, perhaps being unaware of the risks involved in such simplistic assumption. In the present paper, it is also a goal to present a useful and simple formula for approximately estimating the refraction coefficient in terms of easily accessible parameters to correct the bulk of the refraction effect in single observations, always bearing in mind that determination of the refraction coefficient by means of a model may turn out to be somewhat inaccurate, but still better than the blind use of a universal k.The authors are grateful to the editor and the anonymous reviewers for their valuable suggestions, corrections, and comments that helped improve the original manuscript. This research is funded by the Spanish Ministry of Science and Innovation (Grant No. AYA2011-23232).Baselga Moreno, S.; García-Asenjo Villamayor, L.; Garrigues Talens, P. (2014). Practical formulas for the refraction coefficient. Journal of Surveying Engineering. 140(2):1-5. https://doi.org/10.1061/(ASCE)SU.1943-5428.0000124S15140

Relationship between aerodynamic roughness length and bulk sedge leaf area index in a mixed-species boreal mire complex

Leaf area index (LAI) is an important parameter in natural ecosystems, representing the seasonal development of vegetation and photosynthetic potential. However, direct measurement techniques require labor-intensive field campaigns that are usually limited in time, while remote sensing approaches often do not yield reliable estimates. Here we propose that the bulk LAI of sedges (LAI(s)) can be estimated alternatively from a micrometeorological parameter, the aerodynamic roughness length for momentum (z(0)). z(0) can be readily calculated from high-response turbulence and other meteorological data, typically measured continuously and routinely available at ecosystem research sites. The regressions of LAI versus z(0) were obtained using the data from two Finnish natural sites representative of boreal fen and bog ecosystems. LAI(s) was found to be well correlated with z(0) and sedge canopy height. Superior method performance was demonstrated in the fen ecosystem where the sedges make a bigger contribution to overall surface roughness than in bogs.Peer reviewe

Submillimetric GPS distance measurement over short baselines: noise mitigation by global robust estimation

The potential use of GPS technology for precise length determination is currently a topic of extensive research. A prior work was dedicated to submillimetric length determination over short baselines and under ideal conditions of data availability and a clear environment. This paper presents a new computation method suited to the less favourable working conditions that are usually encountered in practice. It is based on both robust estimation theory and the use of an ambiguity-free estimation method. As the experimental comparisons with the standard procedure based on least-squares ambiguity determination show, it provides more stable values and permits results to be obtained significant to the submillimetre level with time spans of a few hours.This research is funded by the Spanish Ministry of Science and Innovation (AYA2011-23232).Baselga Moreno, S.; García-Asenjo Villamayor, L.; Garrigues Talens, P. (2014). Submillimetric GPS distance measurement over short baselines: noise mitigation by global robust estimation. Measurement Science and Technology. 25(10):1-6. https://doi.org/10.1088/0957-0233/25/10/105004S162510Altamimi, Z., Collilieux, X., & Métivier, L. (2011). ITRF2008: an improved solution of the international terrestrial reference frame. Journal of Geodesy, 85(8), 457-473. doi:10.1007/s00190-011-0444-4Amiri-Simkooei, A. R., & Tiberius, C. C. J. M. (2006). Assessing receiver noise using GPS short baseline time series. GPS Solutions, 11(1), 21-35. doi:10.1007/s10291-006-0026-8Baire, Q., Bruyninx, C., Legrand, J., Pottiaux, E., Aerts, W., Defraigne, P., … Chevalier, J. M. (2013). Influence of different GPS receiver antenna calibration models on geodetic positioning. GPS Solutions, 18(4), 529-539. doi:10.1007/s10291-013-0349-1Baselga, S. (2007). Global Optimization Solution of Robust Estimation. Journal of Surveying Engineering, 133(3), 123-128. doi:10.1061/(asce)0733-9453(2007)133:3(123)Baselga, S. (2010). Global optimization applied to GPS positioning by ambiguity functions. Measurement Science and Technology, 21(12), 125102. doi:10.1088/0957-0233/21/12/125102Baselga, S. (2014). Ambiguity-Free Method for Fast and Precise GNSS Differential Positioning. Journal of Surveying Engineering, 140(1), 22-27. doi:10.1061/(asce)su.1943-5428.0000111Baselga, S., & García-Asenjo, L. (2008). GNSS Differential Positioning by Robust Estimation. Journal of Surveying Engineering, 134(1), 21-25. doi:10.1061/(asce)0733-9453(2008)134:1(21)Baselga, S., & García-Asenjo, L. (2008). Multipath Mitigation by Global Robust Estimation. Journal of Navigation, 61(3), 385-392. doi:10.1017/s0373463308004803Baselga, S., García-Asenjo, L., & Garrigues, P. (2013). Submillimetric GPS distance measurement over short baselines: case study in inner consistency. Measurement Science and Technology, 24(7), 075001. doi:10.1088/0957-0233/24/7/075001Dow, J. M., Neilan, R. E., & Rizos, C. (2009). The International GNSS Service in a changing landscape of Global Navigation Satellite Systems. Journal of Geodesy, 83(3-4), 191-198. doi:10.1007/s00190-008-0300-3Griffiths, J., & Ray, J. R. (2012). Sub-daily alias and draconitic errors in the IGS orbits. GPS Solutions, 17(3), 413-422. doi:10.1007/s10291-012-0289-1Huber, P. J. (1981). Robust Statistics. Wiley Series in Probability and Statistics. doi:10.1002/0471725250Koivula, H., Häkli, P., Jokela, J., Buga, A., & Putrimas, R. (2011). GPS Metrology: Bringing Traceable Scale to a Local Crustal Deformation GPS Network. International Association of Geodesy Symposia, 105-112. doi:10.1007/978-3-642-20338-1_13Niu, X., Chen, Q., Zhang, Q., Zhang, H., Niu, J., Chen, K., … Liu, J. (2013). Using Allan variance to analyze the error characteristics of GNSS positioning. GPS Solutions, 18(2), 231-242. doi:10.1007/s10291-013-0324-xRay, J., Altamimi, Z., Collilieux, X., & van Dam, T. (2007). Anomalous harmonics in the spectra of GPS position estimates. GPS Solutions, 12(1), 55-64. doi:10.1007/s10291-007-0067-7Snay, R. A., & Soler, T. (2008). Continuously Operating Reference Station (CORS): History, Applications, and Future Enhancements. Journal of Surveying Engineering, 134(4), 95-104. doi:10.1061/(asce)0733-9453(2008)134:4(95)Wieser, A., & Brunner, F. K. (2002). Short Static GPS Sessions: Robust Estimation Results. GPS Solutions, 5(3), 70-79. doi:10.1007/pl00012901Yang, Y. (1999). Robust estimation of geodetic datum transformation. Journal of Geodesy, 73(5), 268-274. doi:10.1007/s001900050243Yang, Y., Song, L., & Xu, T. (2002). Robust estimator for correlated observations based on bifactor equivalent weights. Journal of Geodesy, 76(6-7), 353-358. doi:10.1007/s00190-002-0256-

Imaging Oxygen Distribution in Marine Sediments. The Importance of Bioturbation and Sediment Heterogeneity

The influence of sediment oxygen heterogeneity, due to bioturbation, on diffusive oxygen flux was investigated. Laboratory experiments were carried out with 3 macrobenthic species presenting different bioturbation behaviour patterns:the polychaetes Nereis diversicolor and Nereis virens, both constructing ventilated galleries in the sediment column, and the gastropod Cyclope neritea, a burrowing species which does not build any structure. Oxygen two-dimensional distribution in sediments was quantified by means of the optical planar optode technique. Diffusive oxygen fluxes (mean and integrated) and a variability index were calculated on the captured oxygen images. All species increased sediment oxygen heterogeneity compared to the controls without animals. This was particularly noticeable with the polychaetes because of the construction of more or less complex burrows. Integrated diffusive oxygen flux increased with oxygen heterogeneity due to the production of interface available for solute exchanges between overlying water and sediments. This work shows that sediment heterogeneity is an important feature of the control of oxygen exchanges at the sediment–water interface

Implementation of sequential cropping into JULESvn5.2 land-surface model

Land-surface models (LSMs) typically simulate a single crop per year in a field or location. However, actual cropping systems are characterized by a succession of distinct crop cycles that are sometimes interspersed with long periods of bare soil. Sequential cropping (also known as multiple or double cropping) is particularly common in tropical regions, where the crop seasons are largely dictated by the main wet season. In this paper, we implement sequential cropping in a branch of the Joint UK Land Environment Simulator (JULES) and demonstrate its use at sites in France and India. We simulate all the crops grown within a year in a field or location in a seamless way to understand how sequential cropping influences the surface fluxes of a land-surface model. We evaluate JULES with sequential cropping in Avignon, France, providing over 15 years of continuous flux observations (a point simulation). We apply JULES with sequential cropping to simulate the rice–wheat rotation in a regional 25 km resolution gridded simulation for the northern Indian states of Uttar Pradesh and Bihar and four single-grid-box simulations across these states, where each simulation is a 25 km grid box. The inclusion of a secondary crop in JULES using the sequential cropping method presented does not change the crop growth or development of the primary crop. During the secondary crop growing period, the carbon and energy fluxes for Avignon and India are modified; they are largely unchanged for the primary crop growing period. For India, the inclusion of a secondary crop using this sequential cropping method affects the available soil moisture in the top 1.0 m throughout the year, with larger fluctuations in sequential crops compared with single-crop simulations even outside the secondary crop growing period. JULES simulates sequential cropping in Avignon, the four India locations and the regional run, representing both crops within one growing season in each of the crop rotations presented. This development is a step forward in the ability of JULES to simulate crops in tropical regions where this cropping system is already prevalent. It also provides the opportunity to assess the potential for other regions to implement sequential cropping as an adaptation to climate change