Synergic effect of metabolic syndrome and lipodystrophy on oxidative stress and inflammation process in treated HIV-patients.

The aim of this study was to assess the effect of lipodystrophy (LD) associated to metabolic syndrome (MS) on oxidative stress and inflammation in a cohort of 243 HIV-infected patients with MS, all of them under three different antiretroviral regimens. We collected immunovirological, biochemical and metabolic data, as well as anthropometric measurements. In addition, cardiovascular risk was also assessed by means of Atherogenic Index of Plasma (API) and Framingham Risk Score. The MS-LD patient set was characterized by a lower initial lymphocyte CD4 count and CD4/CD8 ratio and a higher initial viral load than the group without LD. We also found worse lipidic and glycaemic profiles (with lower HDL-cholesterol and higher triglyceride and glucose levels) in the MS-LD group. BMI, systolic blood pressure and Framingham score were significantly increased compared to MS-Non LD. In addition, patients with MS and LD had significantly higher levels of carbonylated proteins, lipid peroxidation, IL-6 and IL-8, as well as a significant decrease in the levels of leptin, adiponectin and antioxidant activities of catalase, super oxide dismutase and glutathione associated enzymes. In MS-LD HIV-1 patients, a significant negative correlation was found between Framingham Risk Score and the antioxidant biomarkers, however a positive association was found between API and protein-C reactive and carbonylated proteins. Segregating by ART, the above-mentioned conditions were worse within the MS-LD group whose treatment contained protease inhibitors, such as lopinavir. In conclusion, HIV-1 infected patients treated for at least six months, especially with regimens including PIs, showed a worsening of inflammatory process and oxidative stress.This work was supported by the Spanish Ministry of Science and Innovation [SAF 2010-17213]; the Regional Ministry of Health from the Andalusian Government [SAS 111226]; “Miguel Servet Type II” grant [CPI13/00003 to M.I.Q.O. and CPII18/00030 to L.G.S.] from ISCIII, co-funded by the European Regional Development Fund (ERDF); “Nicolas Monardes” research program from the Regional Ministry of Health from the Andalusian Government [C-0030-2018 to M.I.Q.O. and C-0028-2018 to L.G.S.

Salmonella infection in mesenteric lymph nodes of breeding sows

Salmonellosis is one of the main foodborne diseases worldwide. Breeding sows asymptomatically infected with Salmonella can transmit the pathogen to piglets and humans. The isolation of Salmonella from mesenteric lymph nodes (MLNs) is considered a demonstration of asymptomatic infection in swine. As previous breeding sow studies have been performed using feces, the aim of this work was to study the occurrence of Salmonella infections by sampling MLNs, in comparison to their serological status. First, Salmonella fecal shedding was studied in 12/16 large breeding farms to establish the framework of study. Then, MLN (n = 264) and blood (n = 237) samples were obtained at an abattoir from sows of 15 of these 16 breeding farms. Additionally, risk factors associated with Salmonella MLN infection were analyzed. A total of 6.1% (16/264) sows, distributed in 40% (6/15) of the farms, had the pathogen in MLN. Salmonella Typhimurium was the most frequent serovar isolated. Interestingly, 43.8% (7/16) of MLN isolates were susceptible to all the antimicrobials tested and were found distributed throughout all farms with at least one sow positive. As well, one isolate of the emerging DT195 clone was detected and found to be resistant to six antibiotic families (ASSuTNx-Cfx). The serovars and the resistance profiles of the Salmonella isolates from feces were completely different to those obtained from MLNs. The seroprevalence (41.8% of sows and 100% of farms) was higher than that of MLN infections, showing no concordance (k = 0.15) between these two diagnostic tests in sows. Strategies directed to correct two risk factors (i.e., administration of dry food and old premises) would most likely help to reduce Salmonella infections in breeding sows.The work was financed by Departamento de Industria, Energía e Innovación of the Navarra Government [reference IIQ14064.RI1] and Instituto Navarro de Tecnología e Infraestructuras Agroalimentarias S.A. -INTIA- [reference CAM2011030054]. VG, SS, BSR and LMG contracts were funded by UPNA postdoctoral fellowship, Erasmus Mundus EMUNDUS18 Program, CSIC JAE-Doc Program, and INIA-European Social Fund, respectively. We are grateful to farmers, veterinaries and slaughterhouse workers as well as to the temporal students Naroa Remondegui, June Landa (JAE-Intro CSIC-FEDER fellowship), Ruth Erro and Mirian Samblas

Challenge 6: Open Science: reproducibility, transparency and reliability

Open Science is becoming a new paradigm in scientific research and complex changes are being done. This new way in knowledge development requires a great transformation that will allow science to adapt efficiently and effectively to the urgency of the problems to be solved while ensuring the reproducibility, transparency and reliability of scientific results. This chapter analyzes the impact of this change of model, the challenges to be addressed and the expected benefits.Peer reviewe

De Novo Lipogenesis in Adipose Tissue Is Associated with Course of Morbid Obesity after Bariatric Surgery

OBJECTIVE: De novo lipogenesis is involved in fatty acid biosynthesis and could be involved in the regulation of the triglyceride storage capacity of adipose tissue. However, the association between lipogenic and lipolytic genes and the evolution of morbidly obese subjects after bariatric surgery remains unknown. In this prospective study we analyze the association between the improvement in the morbidly obese patients as a result of bariatric surgery and the basal expression of lipogenic and lipolytic genes. METHODS: We study 23 non diabetic morbidly obese patients who were studied before and 7 months after bariatric surgery. Also, we analyze the relative basal mRNA expression levels of lipogenic and lipolytic genes in epiploic visceral adipose tissue (VAT) and abdominal subcutaneous adipose tissue (SAT). RESULTS: When the basal acetyl-CoA carboxylase 1 (ACC1), acetyl-CoA synthetase 2 (ACSS2) and ATP citrate lyase (ACL) expression in SAT was below percentile-50, there was a greater decrease in weight (P = 0.006, P = 0.034, P = 0.026), body mass index (P = 0.008, P = 0.033, P = 0.034) and hip circumference (P = 0.033, P = 0.021, P = 0.083) after bariatric surgery. In VAT, when the basal ACSS2 expression was below percentile-50, there was a greater decrease in hip circumference (P = 0.006). After adjusting for confounding variables in logistic regression models, only the morbidly obese patients with SAT or VAT ACSS2 expression ≥ P50 before bariatric surgery had a lower percentage hip circumference loss (<P50) after bariatric surgery (SAT: P = 0.039; VAT: P = 0.033). CONCLUSIONS: A lower basal ACSS2, ACC1 and ACL expression, genes involved in de novo lipogenesis, is associated with a better evolution of anthropometric variables after bariatric surgery. Thus, the previous state of the pathways involved in fatty acid metabolism may have repercussions on the improvement of these patients

Antiviral capacity of sanitizers against infectious viruses in process water from the produce industry under batch and continuous conditions

The presence of human enteric viruses in produce has extensively been reported. However, the significance of the quality of process water (PW) used by the produce industry and the viral inactivation capacity of water disinfection agents used to maintain the microbiological quality of PW has received limited attention. This study evaluates the antiviral disinfection efficacy of chlorine, chlorine dioxide (ClO2) and peracetic acid (PAA) at recommended operational limits in PW using hepatitis A virus (HAV), the cultivable norovirus surrogate, murine norovirus (MNV-1), and MS2 coliphages. Defined commodity representative crops (baby leaves, bell peppers, and the vegetable mix of tomatoes, cucumbers, peppers, and onions) associated with specific water-based processes were studied. Two systems classified as either batch or continuous system were used. The continuous system allows the continuously entrance of sanitizer solution and organic matter added to the washing tank to simulate the conditions of an industry wash tank. Batch scale experiments showed that 20 mg/L chlorine and 3 mg/L chlorine dioxide completely inactivated MNV-1 and MS2 (mean of 5 log) after 1 min contact time regardless of the PW type. However, the infectivity of HAV was reduced only by less than 2 log after 1 min for chlorine and chlorine dioxide and the complete inactivation was not observed even after 10 min. On the contrary, residual viral infectivity/viability of HAV, MNV-1 and MS2 was observed for PAA in the three types of PW. The inactivation kinetic models for MS2 coliphages were developed based on the data obtained under the continuous system comparing the three types of PW. Chlorine (5 mg/L) and chlorine dioxide (2-3 mg/L) avoided the accumulation of MS2 below the detection limit while PAA (80 mg/L) was unable to prevent it independently of the type of PW. In summary, in the washing operation, it is a key objective to reach virus inactivation through the selection of the most effective sanitizer by guaranteeing that sufficient concentration and contact times prevent the risk of viral cross-contamination

Adipose tissue as a therapeutic target for vascular damage in Alzheimer's disease

Adipose tissue has recently been recognized as an important endocrine organ that plays a crucial role in energy metabolism and in the immune response in many metabolic tissues. With this regard, emerging evidence indicates that an important crosstalk exists between the adipose tissue and the brain. However, the contribution of adipose tissue to the development of age-related diseases, including Alzheimer's disease, remains poorly defined. New studies suggest that the adipose tissue modulates brain function through a range of endogenous biologically active factors known as adipokines, which can cross the blood–brain barrier to reach the target areas in the brain or to regulate the function of the blood–brain barrier. In this review, we discuss the effects of several adipokines on the physiology of the blood–brain barrier, their contribution to the development of Alzheimer's disease and their therapeutic potential.Funding for open access charge; Universidad de Málaga / CBU

Expression of epicardial adipose tissue thermogenic genes in patients with reduced and preserved ejection fraction heart failure

[Abstract] Epicardial adipose tissue has been proposed to participate in the pathogenesis of heart failure. The aim of our study was to assess the expression of thermogenic genes (Uncoupling protein 1 (UCP1), peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1α), and PR-domain-missing 16 (PRDM16) in epicardial adipose tissue in patients with heart failure, stablishing the difference according to left ventricular ejection fraction (reduced or preserved). Among the 75 patients in our study, 42.7% (n=32) had reduced left ventricular ejection fraction. UCP1, PGC1α and PRDM16 mRNA in EAT were significantly lower in patients with reduced left ventricular ejection fraction. Multiple regression analysis showed that age, male gender, body max index, presence of obesity, type-2-diabetes mellitus, hypertension and coronary artery disease and left ventricular ejection fraction were associated with the expression levels of UCP1, PGC1α and PRDM16 mRNA. Thermogenic genes expressions in epicardial adipose tissue (UCP1: OR 0.617, 95%CI 0.103-0.989, p=0.042; PGC1α: OR 0.416, 95%CI 0.171-0.912, p=0.031; PRDM16: OR 0.643, 95%CI 0.116-0.997, p=0.044) were showed as protective factors against the presence of heart failure with reduced left ventricular ejection fraction, and age (OR 1.643, 95%CI 1.001-3.143, p=0.026), presence of coronary artery disease (OR 6.743, 95%CI 1.932-15.301, p<0.001) and type-2-diabetes mellitus (OR 4.031, 95%CI 1.099-7.231, p<0.001) were associated as risk factors. The adequate expression of thermogenic genes has been shown as possible protective factors against heart failure with reduced ejection fraction, suggesting that a loss of functional epicardial adipose tissue brown-like features would participate in a deleterious manner on heart metabolism. Thermogenic genes could represent a future novel therapeutic target in heart failure.Ministerio de Salud, Servicios Sociales e Igualdad; PI13/02542Ministerio de Salud, Servicios Sociales e Igualdad; PI11/01661Red de Investigación Cardiovascular (España), RD12/0042/003

Mitochondrial dysfunction and mitophagy activation in blood mononuclear cells of fibromyalgia patients: implications in the pathogenesis of the disease

This is an open access article distributed under the terms of the Creative Commons Attribution License.[Introduction]: Fibromyalgia is a chronic pain syndrome with unknown etiology. Recent studies have shown some evidence demonstrating that oxidative stress may have a role in the pathophysiology of fibromyalgia. However, it is still not clear whether oxidative stress is the cause or the effect of the abnormalities documented in fibromyalgia. Furthermore, the role of mitochondria in the redox imbalance reported in fibromyalgia also is controversial. We undertook this study to investigate the role of mitochondrial dysfunction, oxidative stress, and mitophagy in fibromyalgia. [Methods]: We studied 20 patients (2 male, 18 female patients) from the database of the Sevillian Fibromyalgia Association and 10 healthy controls. We evaluated mitochondrial function in blood mononuclear cells from fibromyalgia patients measuring, coenzyme Q10 levels with high-performance liquid chromatography (HPLC), and mitochondrial membrane potential with flow cytometry. Oxidative stress was determined by measuring mitochondrial superoxide production with MitoSOX™ and lipid peroxidation in blood mononuclear cells and plasma from fibromyalgia patients. Autophagy activation was evaluated by quantifying the fluorescence intensity of LysoTracker™ Red staining of blood mononuclear cells. Mitophagy was confirmed by measuring citrate synthase activity and electron microscopy examination of blood mononuclear cells. [Results]: We found reduced levels of coenzyme Q10, decreased mitochondrial membrane potential, increased levels of mitochondrial superoxide in blood mononuclear cells, and increased levels of lipid peroxidation in both blood mononuclear cells and plasma from fibromyalgia patients. Mitochondrial dysfunction was also associated with increased expression of autophagic genes and the elimination of dysfunctional mitochondria with mitophagy. [Conclusions]: These findings may support the role of oxidative stress and mitophagy in the pathophysiology of fibromyalgia.This work was supported by grants FIS PI080500 and FIS EC08/00076, Ministerio de Sanidad, Spain. The authors dedicate this manuscript to FM patients and AFIBROSE (Asociación de Fibromialgia de Sevilla) for their unconditional help.Peer Reviewe

Newly impaired glucose metabolism and prognosis after percutaneous revascularization

Background: Clinical practice guidelines recommend ad hoc screening of diabetes in patients admitted for macrovascular disease; however, these recommendations are rarely followed in real practice. This study was undertaken to assess whether impaired glucose metabolism, newly diagnosed after percutaneous coronary intervention (PCI) or known diabetes, provides prognostic information. Methods: We studied 374 patients who underwent PCI. An oral glucose tolerance test was carried out in the known non-diabetic patients with fasting glucose &lt; 7 mmol/L. Results: Eighty-one percent of the patients presented impaired glucose metabolism, from which 35.3% were previously diagnosed with diabetics, 21.4% were newly detected diabetics, and 24.3% were pre-diabetics. After a mean follow-up of 35.8 ± 13.4 months, only a known history of diabetes was an independent predictor of revascularization (OR = 2.03, p = 0.025), non-fatal acute myocardial infarction (OR = 2.70, p = 0.029) and readmission due to heart failure during the follow-up (OR = 3.82, p = 0.022). Conclusions: Screening for impaired glucose metabolism after PCI permits the detection of a high proportion of patients with abnormal glucose regulations. However, previously known diabetes remains the only independent predictor of cardiovascular events in the follow-up.

The AMIGA sample of isolated galaxies XIV. Disc breaks and interactions through ultra-deep optical imaging

Context. In the standard cosmological model of galaxy evolution, mergers and interactions play a fundamental role in shaping galaxies. Galaxies that are currently isolated are thus interesting because they allow us to distinguish between internal and external processes that affect the galactic structure. However, current observational limits may obscure crucial information in the low-mass or low-brightness regime. Aims. We use optical imaging of a subsample of the AMIGA catalogue of isolated galaxies to explore the impact of different factors on the structure of these galaxies. In particular, we study the type of disc break as a function of the degree of isolation and the presence of interaction indicators such as tidal streams or plumes, which are only detectable in the ultra-low surface brightness regime. Methods. We present ultra-deep optical imaging in the r band of a sample of 25 low-redshift (z< 0.035) isolated galaxies. Through careful data processing and analysis techniques, the nominal surface brightness limits achieved are comparable to those to be obtained on the ten-year LSST coadds (μr,lim ≳ 29.5 mag arcsec-2 [3σ; 10'' × 10'']). We place special emphasis on preserving the low surface brightness features throughout the processing. Results. The extreme depth of our imaging allows us to study the interaction signatures of 20 galaxies since Galactic cirrus is a strong limiting factor in the characterisation of interactions for the remaining 5 of them. We detect previously unreported interaction features in 8 (40% ± 14%) galaxies in our sample. We identify 9 galaxies (36% ± 10%) with an exponential disc (Type I), 14 galaxies (56% ± 10%) with a down-bending (Type II) profile, and only 2 galaxies (8% ± 5%) with up-bending (Type III) profiles. Isolated galaxies have considerably more purely exponential discs and fewer up-bending surface brightness profiles than field or cluster galaxies. We find clear minor merger activity in some of the galaxies with single exponential or down-bending profiles, and both of the galaxies with up-bending profiles show signatures of a past interaction. Conclusions. We show the importance of ultra-deep optical imaging in revealing faint external features in galaxies that indicate a probable history of interaction. We confirm that up-bending profiles are likely produced by major mergers, while down-bending profiles are probably formed by a threshold in star formation. Unperturbed galaxies that slowly evolve with a low star formation rate could induce the high rate of Type I discs in isolated galaxies. © The Authors 2023.We thank Ignacio Trujillo for helpful insights about this work and Aaron Watkins for providing us with the implementation of the automatic break detection method. PMSA, JHK, and JR acknowledge financial support from the State Research Agency (AEI-MCINN) of the Spanish Ministry of Science and Innovation under the grant "The structure and evolution of galaxies and their central regions" with reference PID2019-105602GBI00/10.13039/501100011033, from the ACIISI, Consejeria de Economia, Conocimiento y Empleo del Gobierno de Canarias and the European Regional Development Fund (ERDF) under grant with reference PROID2021010044, and from IAC project P/300724, financed by the Ministry of Science and Innovation, through the State Budget and by the Canary Islands Department of Economy, Knowledge and Employment, through the Regional Budget of the Autonomous Community. JR acknowledges funding from University of La Laguna through the Margarita Salas Program from the Spanish Ministry of Universities ref. UNI/551/2021-May 26, and under the EU Next Generation. LVM acknowledges financial support from grants CEX2021-001131-S funded by MCIN/AEI/ 10.13039/501100011033, RTI2018-096228-B-C31 and PID2021-123930OB-C21 by MCIN/AEI/ 10.13039/501100011033, by "ERDF A way of making Europe" and by the "European Union" and from IAA4SKA (R18-RT-3082) funded by the Economic Transformation, Industry, Knowledge and Universities Council of the Regional Government of Andalusia and the European Regional Development Fund from the European Union. SC acknowledges funding from the State Research Agency (AEI-MCINN) of the Spanish Ministry of Science and Innovation under the grant "Thick discs, relics of the infancy of galaxies" with reference PID2020-113213GA-I00. MAF acknowledges support from FONDECYT iniciacion project 11200107 and the Emergia program (EMERGIA20_38888) from Consejeria de Transformacion Economica, Industria, Conocimiento y Universidades and University of Granada. PMSA and LVM acknowledge the Spanish Prototype of an SRC (SPSRC) service and support funded by the Spanish Ministry of Science, Innovation and Universities, by the Regional Government of Andalusia, by the European Regional Development Funds and by the European Union NextGenerationEU/PRTR. The SPSRC acknowledges financial support from the State Agency for Research of the Spanish MCIU through the "Center of Excellence Severo Ochoa" award to the Instituto de Astrofisica de Andalucia (SEV-2017-0709) and from the grant CEX2021-001131-S funded by MCIN/AEI/ 10.13039/501100011033. Based on observations made with the Isaac Newton Telescope operated on the island of La Palma by the Isaac Newton Group of Telescopes in the Spanish Observatorio del Roque de los Muchachos of the Instituto de Astrofisica de Canarias. The WFC imaging was obtained as part of the programs C163/13A, C106/13B, and C106/14A. Based on observations collected at the European Organisation for Astronomical Research in the Southern Hemisphere under ESO programme(s) 098.B-0775(A), 093.B-0894(A). Based on data collected at the Subaru Telescope and retrieved from the HSC data archive system, which is operated by Subaru Telescope and Astronomy Data Center at National Astronomical Observatory of Japan