Efficacy comparison of various oxygen lnhibition layer (OIL) minimizing agents on composite resin by analysis of two different physical properties: An in vitro study

Introduction: Composite resins are the most common restorative materials that are used these days and have been radically improving over the past few years. Their polymerisation reaction can be inhibited during light-curing due to the oxygen presence in atmosphere. Aim/objective: The aim of this study is to evaluate effect of different air inhibition coating strategies on composite resin material in terms of hardness and discolouration. Material and Methodology: Composite discs of 6.5 X 2mm were prepared for samples which were cured under a) 0.5mm thick mylar strip b) thin layer of glycerine c) layer of KY jelly (commercial lubricant) d) air cure. These samples were dipped in cola, turmeric milk, coffee and distilled water for 14 days. Spectrophotometric analysis was done to assess the colour change and Vicker’s hardness test for assessment of the hardness. Statistical analysis was done and the results were concluded. Results: The results were analyzed with ANOVA one-way test followed by Student Newman-Keul test. Moreover, multiple comparisons of means were performed using the Student t-test (p<0.05). Conclusion: Presence of Oxygen inhibition layer(OIL) on surface layer of composite affected the specimens adversely by reducing the microhardness and increasing instability of colour

Efficacy Comparison of Various Oxygen Lnhibition Layer (OIL) Minimizing Agents on Composite Resin by Analysis of Two Different Physical Properties: an in Vitro Study

Introduction: Composite resins are the most common restorative materials that are used these days and have been radically improving over the past few years. Their polymerisation reaction can be inhibited during light-curing due to the oxygen presence in atmosphere. Aim/objective: The aim of this study is to evaluate effect of different air inhibition coating strategies on composite resin material in terms of hardness and discolouration. Material and Methodology: Composite discs of 6.5 X 2mm were prepared for samples which were cured under a) 0.5mm thick mylar strip b) thin layer of glycerine c) layer of KY jelly (commercial lubricant) d) air cure. These samples were dipped in cola, turmeric milk, coffee and distilled water for 14 days. Spectrophotometric analysis was done to assess the colour change and Vicker's hardness test for assessment of the hardness. Statistical analysis was done and the results were concluded. Results: The results were analyzed with ANOVA one-way test followed by Student Newman-Keul test. Moreover, multiple comparisons of means were performed using the Student t-test (p<0.05). Conclusion: Presence of Oxygen inhibition layer(OIL) on surface layer of composite affected the specimens adversely by reducing the microhardness and increasing instability of colour

Differences in DNA condensation and release by lysine and arginine homopeptides govern their DNA delivery efficiencies

Designing of nanocarriers that can efficiently deliver therapeutic DNA payload and allow its smooth intracellular release for transgene expression is still a major constraint. The optimization of DNA nanocarriers requires thorough understanding of the chemical and structural characteristics of the vector-nucleic acid complexes and its correlation with the cellular entry, intracellular state and transfection efficiency. l-Lysine and l-arginine based cationic peptides alone or in conjugation with other vectors are known to be putative DNA delivery agents. Here we have used l-lysine and l-arginine homopeptides of three different lengths and probed their DNA condensation and release properties by using a multitude of biophysical techniques including fluorescence spectroscopy, gel electrophoresis and atomic force microscopy. Our results clearly showed that although both lysine and arginine based homopeptides condense DNA via electrostatic interactions, they follow different pattern of DNA condensation and release in vitro. While lysine homopeptides condense DNA to form both monomolecular and multimolecular complexes and show differential release of DNA in vitro depending on the peptide length, arginine homopeptides predominantly form multimolecular complexes and show complete DNA release for all peptide lengths. The cellular uptake of the complexes and their intracellular state (as observed through flow cytometry and fluorescence microscopy) seem to be controlled by the peptide chemistry. The difference in the transfection efficiency of lysine and arginine homopeptides has been rationalized in light of these observations

Microalgal Biomass of Industrial Interest: Methods of Characterization

International audienceMicroalgae represent a new source of biomass for many applications. The advantage of microalgae over higher plants is their high productivities. The photoautotrophic microalgae include all photosynthetic microorganisms, i.e. Cyanobacteria (prokaryotes) or microalgae (eukaryotes). These microorganisms are characterized by a large biodiversity and chimiodiversity. Then, the analysis of microalgal and cyanobacterial biomass often needs specific adaptations of the classical protocols for extraction as well as for quantification of their contents. This chapter reviewed the main analytical methods used for the analysis of microalgae biomass and its main vaporizable compounds: proteins, polysaccharides, lipids, pigments and secondary metabolites