Ventilatory threshold concordance between ergoespirometry and heart rate variability in female professional cyclists

The ventilatory threshold (VT) is the point at which ventilation intensifies disproportionately concerning to the oxygen uptake, this parameter is essential within the training methodology to optimize performance; the purpose of this work is to measure the degree of agreement between the determination of the ventilatory threshold determined by for oxygen uptake and four methods by Heart Rate Variability (HRV). Methodology: Twelve professional female cyclists between 18 and 28 years of age were evaluated through a maximal incremental test on a cycle ergometer; gas exchange was measure with a portable telemetric device (Cosmed K4b2®) and simultaneously the HRV was registered by R-R recording trough a Polar RS800CX® (Polar, Finland) heart rate monitor. Statistics: The degree of precision and accuracy established between the variables mentioned and normality test Shapiro-Wilk. Results: rho = 1.00 with p-value < .05, and Lin correlation and concordance coefficients of .99 with r = 0.98 for ventilatory threshold 2 (VT2), constituting a level of precision and accuracy almost perfect; by contrast, ventilatory threshold 1 (VT1) did not show significant precision and accuracy. This study suggests that VT2 can be identified from The time series. RR using the analysis of frequency and temporal domains likewise allows. Us to have a reference measure for future research in other sports.The project was funded through the internal of the Institución Universitaria Escuela Nacional del Deporte

Comparative Genomic Analysis of Human Fungal Pathogens Causing Paracoccidioidomycosis

Paracoccidioides is a fungal pathogen and the cause of paracoccidioidomycosis, a health-threatening human systemic mycosis endemic to Latin America. Infection by Paracoccidioides, a dimorphic fungus in the order Onygenales, is coupled with a thermally regulated transition from a soil-dwelling filamentous form to a yeast-like pathogenic form. To better understand the genetic basis of growth and pathogenicity in Paracoccidioides, we sequenced the genomes of two strains of Paracoccidioides brasiliensis (Pb03 and Pb18) and one strain of Paracoccidioides lutzii (Pb01). These genomes range in size from 29.1 Mb to 32.9 Mb and encode 7,610 to 8,130 genes. To enable genetic studies, we mapped 94% of the P. brasiliensis Pb18 assembly onto five chromosomes. We characterized gene family content across Onygenales and related fungi, and within Paracoccidioides we found expansions of the fungal-specific kinase family FunK1. Additionally, the Onygenales have lost many genes involved in carbohydrate metabolism and fewer genes involved in protein metabolism, resulting in a higher ratio of proteases to carbohydrate active enzymes in the Onygenales than their relatives. To determine if gene content correlated with growth on different substrates, we screened the non-pathogenic onygenale Uncinocarpus reesii, which has orthologs for 91% of Paracoccidioides metabolic genes, for growth on 190 carbon sources. U. reesii showed growth on a limited range of carbohydrates, primarily basic plant sugars and cell wall components; this suggests that Onygenales, including dimorphic fungi, can degrade cellulosic plant material in the soil. In addition, U. reesii grew on gelatin and a wide range of dipeptides and amino acids, indicating a preference for proteinaceous growth substrates over carbohydrates, which may enable these fungi to also degrade animal biomass. These capabilities for degrading plant and animal substrates suggest a duality in lifestyle that could enable pathogenic species of Onygenales to transfer from soil to animal hosts.National Institute of Allergy and Infectious Diseases (U.S.)National Institutes of Health. Department of Health and Human Services (contract HHSN266200400001C)National Institutes of Health. Department of Health and Human Services(contract HHSN2722009000018C)Brazil. National Council for Scientific and Technological Developmen

Sequence and phylogenetic analysis of human T cell lymphotropic virus type 1 from Tumaco, Colombia

Human T cell lymphotropic virus type 1 (HTLV-1) is a retrovirus that causes leukemia and the neurological disorder HTLV-1 associated myelopathy or tropical spastic paraparesis (HAM/TSP). Infection with this virus - although it is distributed worldwide - is limited to certain endemic areas of the world. Despite its specific distribution and slow mutation rate, molecular epidemiology on this virus has been useful to follow the movements of human populations and routes of virus spread to different continents. In the present study, we analyzed the genetic variability of a region of the env gene of isolates obtained from individuals of African origin that live on the Pacific coast of Colombia. Sequencing and comparison of the fragment with the same fragment from different HTLV-1 isolates showed a variability ranging from 0.8% to 1.2%. Phylogenetic studies permit us to include these isolates in the transcontinental subgroup A in which samples isolated from Brazil and Chile are also found. Further analyses will be necessary to determine if these isolates were recently introduced into the American continent or if they rather correspond to isolates introduced during the Paleolithic period

Muscle genomics and aerobic training

The performance in physical activity is determined not only by physiological processes such as age, body composition, gender and degree of training, but also by the genomics and even epigenetic events occurring during the training programs. In this context, using bioinformatics resources, we aimed to analyse the expression of genes associated with muscle function in vastus lateral samples. We used data from DNA microarray experiments reported in NCBI's GEO DataSet database under the series number GSE117070. Differential expression was calculated using the Z-ratio equation. We also used the software Cytoscape 3.6 to build a protein-protein interaction network with over-expressed genes. We found that seven genes out of the 397 genes analysed in the 41 individuals subjected to aerobic exercise with an increase in training intensity through the percentage of VO2max, were over-expressed based on the statistical approach. The Protein-Protein Interaction (PPI) network showed 477 nodes, two connected components, 17 multi-edge node pairs and an average number of neighbours of 2.092. The node with the highest number of interactions was TPM1 with 150. GO categories of biological processes most relevant of the network included indispensable processes for muscle function and contraction such as polymerization of actin filaments and ATP synthesis from electron transport chain.The project was funded by the vice-rectory of research at the Universidad del Valle through the scholarship 119-2019

GOECP/SEOR radiotheraphy guidelines for non-small-cell lung cancer

Non-small cell lung cancer (NSCLC) is a heterogeneous disease accounting for approximately 85% of all lung cancers. Only 17% of patients are diagnosed at an early stage. Treatment is multidisciplinary and radiotherapy plays a key role in all stages of the disease. More than 50% of patients with NSCLC are treated with radiotherapy (curative-intent or palliative). Technological advances-including highly conformal radiotherapy techniques, new immobilization and respiratory control systems, and precision image verification systems-allow clinicians to individualize treatment to maximize tumor control while minimizing treatment-related toxicity. Novel therapeutic regimens such as moderate hypofractionation and advanced techniques such as stereotactic body radiotherapy (SBRT) have reduced the number of radiotherapy sessions. The integration of SBRT into routine clinical practice has radically altered treatment of early-stage disease. SBRT also plays an increasingly important role in oligometastatic disease. The aim of the present guidelines is to review the role of radiotherapy in the treatment of localized, locally-advanced, and metastatic NSCLC. We review the main radiotherapy techniques and clarify the role of radiotherapy in routine clinical practice. These guidelines are based on the best available evidence. The level and grade of evidence supporting each recommendation is provided