Carcinoma gástrico en el perro: localización endoscópica y aspecto macroscópico

El propósito de este trabajo es el estudio de las localizaciones endoscópicas y aspecto macroscópico del carcinoma gástrico canino. Se han revisado las historias clínicas de 17 perros sometidos a endoscopia digestiva superior a los que se les ha diagnosticado un carcinoma gástrico. Los resultados del estudio señalan que el antro pilórico y la curvatura menor son las localizaciones más frecuentes del carcinoma gástrico canino: el 64,7%% de los casos presentaban afectación de antro pilórico, aunque muchos de ellos se extendían además a otras zonas del estómago; por otro lado, el 47% de los carcinomas estudiados afectaban a curvatura menor, aunque sólo el 11,8% se limitaban a esa zona.The aim of this paper is to study endoscopic localizations and macroscopic appearance of canine gastric carcinoma. Clinical histories of seventeen dogs were analyzed. All cases were diagnosed of gastric carcinoma by Upper Digestive Endoscopy and Biopsy. The results of this study show that pyloric antrum and lesser curvature are the most frequent locations of canine gastric carcinoma: in 64.7% of the cases pyloric antrum was affected, even though some of them were extended to other zones of the stomach as well; on the other hand, 47% of the studied cases affected the lesser curvature, but only 11.8% were limited to that location

Plasma-Induced Defects Enhance the Visible-Light Photocatalytic Activity of MIL-125(Ti)-NH2 for Overall Water Splitting

This is the peer reviewed version of the following article: M. Cabrero-Antonino, J. Albero, C. García-Vallés, M. Álvaro, S. Navalón, H. García, Chem. Eur. J. 2020, 26, 15682, which has been published in final form at https://doi.org/10.1002/chem.202003763. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.[EN] Defect engineering in metal-organic frameworks is commonly performed by using thermal or chemical treatments. Herein we report that oxygen plasma treatment generates structural defects on MIL-125(Ti)-NH2, leading to an increase in its photocatalytic activity. Characterization data indicate that plasma-treated materials retain most of their initial crystallinity, while exhibiting somewhat lower surface area and pore volume. XPS and FT-IR spectroscopy reveal that oxygen plasma induces MIL-125(Ti)-NH2 partial terephthalate decarboxylation and an increase in the Ti-OH population. Thermogravimetric analyses confirm the generation of structural defects by oxygen plasma and allowed an estimation of the resulting experimental formula of the treated MIL-125(Ti)-NH2 solids. SEM analyses show that oxygen plasma treatment of MIL-125(Ti)-NH2 gradually decreases its particle size. Importantly, diffuse reflectance UV/Vis spectroscopy and valence band measurements demonstrate that oxygen plasma treatment alters the MIL-125(Ti)-NH2 band gap and, more significantly, the alignment of highest occupied and lowest unoccupied crystal orbitals. An optimal oxygen plasma treatment to achieve the highest efficiency in water splitting with or without methanol as sacrificial electron donor under UV/Vis or simulated sunlight was determined. The optimized plasma-treated MIL-125(Ti)-NH2 photocatalyst acts as a truly heterogeneous photocatalyst and retains most of its initial photoactivity and crystallinity upon reuse.S.N. thanks the Fundacion Ramon Areces (XVIII Concurso Nacional para la Adjudicacion de Ayudas a la Investigacion en Ciencias de la Vida y de la Materia, 2016), the Ministerio de Ciencia, Innovacion y Universidades RTI 2018-099482-A-I00 project, the Generalitat Valenciana grupos de investigacion consolidables 2019 (ref: AICO/2019/214) project, and the AVI project (INNEST/2020/111) for financial support. Financial support by the European Union (LoterCO2M), Spanish Ministry of Science, Innovation and Universities (Severo Ochoa and RTI2018-098237-B-C21), and Generalitat Valenciana (Prometeo 2017-083) is also gratefully acknowledged.Cabrero-Antonino, M.; Albero-Sancho, J.; García-Vallés, C.; Alvaro Rodríguez, MM.; Navalón Oltra, S.; García Gómez, H. (2020). Plasma-Induced Defects Enhance the Visible-Light Photocatalytic Activity of MIL-125(Ti)-NH2 for Overall Water Splitting. Chemistry - A European Journal. 26(67):15682-15689. https://doi.org/10.1002/chem.202003763S15682156892667Dhakshinamoorthy, A., Asiri, A. M., & García, H. (2016). Metal–Organic Framework (MOF) Compounds: Photocatalysts for Redox Reactions and Solar Fuel Production. Angewandte Chemie International Edition, 55(18), 5414-5445. doi:10.1002/anie.201505581Dhakshinamoorthy, A., Asiri, A. M., & Garcia, H. (2016). Metall‐organische Gerüstverbindungen: Photokatalysatoren für Redoxreaktion und die Produktion von Solarbrennstoffen. Angewandte Chemie, 128(18), 5504-5535. doi:10.1002/ange.201505581Dhakshinamoorthy, A., Li, Z., & Garcia, H. (2018). Catalysis and photocatalysis by metal organic frameworks. Chemical Society Reviews, 47(22), 8134-8172. doi:10.1039/c8cs00256hZhang, T., & Lin, W. (2014). Metal–organic frameworks for artificial photosynthesis and photocatalysis. Chem. Soc. Rev., 43(16), 5982-5993. doi:10.1039/c4cs00103fWang, J.-L., Wang, C., & Lin, W. (2012). Metal–Organic Frameworks for Light Harvesting and Photocatalysis. ACS Catalysis, 2(12), 2630-2640. doi:10.1021/cs3005874Wen, M., Mori, K., Kuwahara, Y., An, T., & Yamashita, H. (2018). Design of Single-Site Photocatalysts by Using Metal-Organic Frameworks as a Matrix. Chemistry - An Asian Journal, 13(14), 1767-1779. doi:10.1002/asia.201800444Ding, M., Flaig, R. W., Jiang, H.-L., & Yaghi, O. M. (2019). Carbon capture and conversion using metal–organic frameworks and MOF-based materials. Chemical Society Reviews, 48(10), 2783-2828. doi:10.1039/c8cs00829aLi, D., Kassymova, M., Cai, X., Zang, S.-Q., & Jiang, H.-L. (2020). Photocatalytic CO2 reduction over metal-organic framework-based materials. Coordination Chemistry Reviews, 412, 213262. doi:10.1016/j.ccr.2020.213262Zhang, T., Jin, Y., Shi, Y., Li, M., Li, J., & Duan, C. (2019). Modulating photoelectronic performance of metal–organic frameworks for premium photocatalysis. Coordination Chemistry Reviews, 380, 201-229. doi:10.1016/j.ccr.2018.10.001Luo, H., Zeng, Z., Zeng, G., Zhang, C., Xiao, R., Huang, D., … Tian, S. (2020). Recent progress on metal-organic frameworks based- and derived-photocatalysts for water splitting. Chemical Engineering Journal, 383, 123196. doi:10.1016/j.cej.2019.123196Schneider, J., Matsuoka, M., Takeuchi, M., Zhang, J., Horiuchi, Y., Anpo, M., & Bahnemann, D. W. (2014). Understanding TiO2 Photocatalysis: Mechanisms and Materials. Chemical Reviews, 114(19), 9919-9986. doi:10.1021/cr5001892Fujishima, A., Rao, T. N., & Tryk, D. A. (2000). Titanium dioxide photocatalysis. Journal of Photochemistry and Photobiology C: Photochemistry Reviews, 1(1), 1-21. doi:10.1016/s1389-5567(00)00002-2FUJISHIMA, A., ZHANG, X., & TRYK, D. (2008). TiO2 photocatalysis and related surface phenomena. Surface Science Reports, 63(12), 515-582. doi:10.1016/j.surfrep.2008.10.001Zhu, J., Li, P.-Z., Guo, W., Zhao, Y., & Zou, R. (2018). Titanium-based metal–organic frameworks for photocatalytic applications. Coordination Chemistry Reviews, 359, 80-101. doi:10.1016/j.ccr.2017.12.013Chen, X., Peng, X., Jiang, L., Yuan, X., Yu, H., Wang, H., … Xia, Q. (2020). Recent advances in titanium metal–organic frameworks and their derived materials: Features, fabrication, and photocatalytic applications. Chemical Engineering Journal, 395, 125080. doi:10.1016/j.cej.2020.125080Remiro-Buenamañana, S., Cabrero-Antonino, M., Martínez-Guanter, M., Álvaro, M., Navalón, S., & García, H. (2019). Influence of co-catalysts on the photocatalytic activity of MIL-125(Ti)-NH2 in the overall water splitting. Applied Catalysis B: Environmental, 254, 677-684. doi:10.1016/j.apcatb.2019.05.027An, Y., Xu, B., Liu, Y., Wang, Z., Wang, P., Dai, Y., … Huang, B. (2017). Photocatalytic Overall Water Splitting over MIL-125(Ti) upon CoPi and Pt Co-catalyst Deposition. ChemistryOpen, 6(6), 701-705. doi:10.1002/open.201700100Hendon, C. H., Tiana, D., Fontecave, M., Sanchez, C., D’arras, L., Sassoye, C., … Walsh, A. (2013). Engineering the Optical Response of the Titanium-MIL-125 Metal–Organic Framework through Ligand Functionalization. Journal of the American Chemical Society, 135(30), 10942-10945. doi:10.1021/ja405350uHisatomi, T., & Domen, K. (2019). Reaction systems for solar hydrogen production via water splitting with particulate semiconductor photocatalysts. Nature Catalysis, 2(5), 387-399. doi:10.1038/s41929-019-0242-6Nasalevich, M. A., Hendon, C. H., Santaclara, J. G., Svane, K., van der Linden, B., Veber, S. L., … Gascon, J. (2016). Electronic origins of photocatalytic activity in d0 metal organic frameworks. Scientific Reports, 6(1). doi:10.1038/srep23676Ma, X., Wang, L., Zhang, Q., & Jiang, H. (2019). Switching on the Photocatalysis of Metal–Organic Frameworks by Engineering Structural Defects. Angewandte Chemie International Edition, 58(35), 12175-12179. doi:10.1002/anie.201907074Ma, X., Wang, L., Zhang, Q., & Jiang, H. (2019). Switching on the Photocatalysis of Metal–Organic Frameworks by Engineering Structural Defects. Angewandte Chemie, 131(35), 12303-12307. doi:10.1002/ange.201907074De Vos, A., Hendrickx, K., Van Der Voort, P., Van Speybroeck, V., & Lejaeghere, K. (2017). Missing Linkers: An Alternative Pathway to UiO-66 Electronic Structure Engineering. Chemistry of Materials, 29(7), 3006-3019. doi:10.1021/acs.chemmater.6b05444Taddei, M., Schukraft, G. M., Warwick, M. E. A., Tiana, D., McPherson, M. J., Jones, D. R., & Petit, C. (2019). Band gap modulation in zirconium-based metal–organic frameworks by defect engineering. Journal of Materials Chemistry A, 7(41), 23781-23786. doi:10.1039/c9ta05216jSvane, K. L., Bristow, J. K., Gale, J. D., & Walsh, A. (2018). Vacancy defect configurations in the metal–organic framework UiO-66: energetics and electronic structure. Journal of Materials Chemistry A, 6(18), 8507-8513. doi:10.1039/c7ta11155jHendrickx, K., Vanpoucke, D. E. P., Leus, K., Lejaeghere, K., Van Yperen-De Deyne, A., Van Speybroeck, V., … Hemelsoet, K. (2015). Understanding Intrinsic Light Absorption Properties of UiO-66 Frameworks: A Combined Theoretical and Experimental Study. Inorganic Chemistry, 54(22), 10701-10710. doi:10.1021/acs.inorgchem.5b01593Wang, Z., Zhang, Y., Neyts, E. C., Cao, X., Zhang, X., Jang, B. W.-L., & Liu, C. (2018). Catalyst Preparation with Plasmas: How Does It Work? ACS Catalysis, 8(3), 2093-2110. doi:10.1021/acscatal.7b03723Jiang, Z., Ge, L., Zhuang, L., Li, M., Wang, Z., & Zhu, Z. (2019). Fine-Tuning the Coordinatively Unsaturated Metal Sites of Metal–Organic Frameworks by Plasma Engraving for Enhanced Electrocatalytic Activity. ACS Applied Materials & Interfaces, 11(47), 44300-44307. doi:10.1021/acsami.9b15794Xiang, W., Ren, J., Chen, S., Shen, C., Chen, Y., Zhang, M., & Liu, C. (2020). The metal–organic framework UiO-66 with missing-linker defects: A highly active catalyst for carbon dioxide cycloaddition. Applied Energy, 277, 115560. doi:10.1016/j.apenergy.2020.115560Primo, A., Franconetti, A., Magureanu, M., Mandache, N. B., Bucur, C., Rizescu, C., … Garcia, H. (2018). Engineering active sites on reduced graphene oxide by hydrogen plasma irradiation: mimicking bifunctional metal/supported catalysts in hydrogenation reactions. Green Chemistry, 20(11), 2611-2623. doi:10.1039/c7gc03397dGuo, Y., Gao, X., Zhang, C., Wu, Y., Chang, X., Wang, T., … Li, X. (2019). Plasma modification of a Ni based metal–organic framework for efficient hydrogen evolution. Journal of Materials Chemistry A, 7(14), 8129-8135. doi:10.1039/c9ta00696fDan-Hardi, M., Serre, C., Frot, T., Rozes, L., Maurin, G., Sanchez, C., & Férey, G. (2009). A New Photoactive Crystalline Highly Porous Titanium(IV) Dicarboxylate. Journal of the American Chemical Society, 131(31), 10857-10859. doi:10.1021/ja903726mPeng, Y., Rendón-Patiño, A., Franconetti, A., Albero, J., Primo, A., & García, H. (2020). Photocatalytic Overall Water Splitting Activity of Templateless Structured Graphitic Nanoparticles Obtained from Cyclodextrins. ACS Applied Energy Materials, 3(7), 6623-6632. doi:10.1021/acsaem.0c00789Melillo, A., Cabrero-Antonino, M., Navalón, S., Álvaro, M., Ferrer, B., & García, H. (2020). Enhancing visible-light photocatalytic activity for overall water splitting in UiO-66 by controlling metal node composition. Applied Catalysis B: Environmental, 278, 119345. doi:10.1016/j.apcatb.2020.119345Ebbesen, T. W., & Ferraudi, G. (1983). Photochemistry of methyl viologen in aqueous and methanolic solutions. The Journal of Physical Chemistry, 87(19), 3717-3721. doi:10.1021/j100242a028García, H., & Navalón, S. (Eds.). (2018). 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Hacia la calidad en el abordaje del TFG: propuestas de las Facultades de Ciencias Sociales y de la Comunicación y de Ciencias del Trabajo de la Universidad de Cádiz

La reforma de los planes de estudios para la adaptación al Espacio Europeo de Educación Superior (EEES), -RD 1993/2008 y su modificación a través del RD 861 del 3 de mayo de 2010-, ha traído de la mano un profundo cambio del contenido y la orientación práctica de las titulaciones. En particular, una de las modificaciones más importantes para los estudios relacionados con las Ciencias Sociales, tales como Marketing e Investigación de Mercados, Turismo, Gestión y Administración Pública, Publicidad y Relaciones Públicas y Trabajo Social, es la exigencia de un Trabajo Fin de Grado (en adelante, TFG). Lo anterior ha exigido el desarrollo, desde las universidades, de estrategias para su implantación y en la búsqueda de mejores prácticas, calidad y excelencia. Este trabajo muestra las experiencias de innovación docente desarrolladas desde las Facultades de Ciencias del Trabajo y Ciencias Sociales y de la Comunicación de la Universidad de Cádiz desde el año 2017.Páginas 501 a 513

Revisión de los materiales utilizados en el reverso de pintura arrancada aplicados al strappo de esmaltes en aerosol

Tradicionalmente han sido utilizados diversos materiales para reforzar los reversos de pintura arrancada, sobre todo en el caso del Strappo, ya que al tratarse del arranque exclusivo de la película pictórica, ésta necesita de un adhesivo con una alta capacidad adherente que favorezca la unión entre la tela de refuerzo por el reverso y la pintura. Pero el tratamiento de los arranques se complica a la hora de plantear el refuerzo en técnicas pictóricas contemporáneas, como sucede en el caso de strappo de pinturas en aerosol. Como base de investigación, en los últimos años se han realizado diferentes pruebas de refuerzo, utilizando un total de 8 variantes de consolidantes, aplicados sobre el reverso de grafitis arrancados por medio de strappo. Todo ello se realizó atendiendo a la posibilidad de conservar grafitis, a la naturaleza de la técnica pictórica y al empleo de materiales en restauración de pintura mural tradicional.Amor García, RL.; Soriano Sancho, MP.; Sánchez Pons, M. (2011). Revisión de los materiales utilizados en el reverso de pintura arrancada aplicados al strappo de esmaltes en aerosol. Arché. (6):11-16. http://hdl.handle.net/10251/330401116

Influence of HLA Matching on the Efficacy of Allogeneic Mesenchymal Stromal Cell Therapies for Osteoarthritis and Degenerative Disc Disease

Producción CientíficaThe necessity for more effective therapies for chronic osteoarticular diseases has led to the development of treatments based on mesenchymal stem cells (MSCs), the natural precursors of musculoskeletal tissue. Treatments with autologous MSCs yielded excellent results, with nearly 70% improvement of pain and disability in osteoarthritis and degenerative disc disease. Using allogeneic MSCs is logistically more convenient and would widen the pool of eligible patients, but potential immune rejection should be considered. In this context, MSCs are purportedly immune evasive and better tolerated than other cell types. Methods We used samples collected during the performance of 2 randomized clinical trials using allogeneic bone marrow MSCs for treatment of osteoarthritis (NCT01586312) and degenerative disc disease (NCT01860417). Serum samples were used to determine anti-HLA antibodies, whereas either blood or MSC samples were used for HLA typing of recipients and donors, respectively. Algofunctional indexes were used as indicators of clinical evolution, and the correlation between the number of donor-host HLA mismatches and the efficacy of treatment was determined. Results Immune response was weak and transient, with reactivity decaying during the first year. Consistently, better donor-recipient HLA matching did not enhance efficacy. Conclusions This lack of reactivity is presumably due to the cooperation of 2 factors, (1) downregulation of the host immune responses by the transplanted MSCs and (2) effective insulation of these cells inside the articular cavity or the intervertebral disc, respectively. Interestingly, better HLA matching did not enhance efficacy. These observations have medical relevance as they support the clinical use of allogeneic cells, at least as a single-dose administration. Multiple-dose applications will require further research to exclude possible sensitization.Red de Terapia Celular of the Instituto de Salud Carlos III (RD16/0011/0003) and Centro en Red de Medicina Regenerativa de Castilla y Leó

Long-Term Photostability in Terephthalate Metal-Organic Frameworks

This is the peer reviewed version of the following article: Mateo, Diego, et al. Long-Term Photostability in Terephthalate Metal-Organic Frameworks. Angewandte Chemie (International Ed.), vol. 58, no. 49, Wiley, 2019, pp. 17843 48, doi:10.1002/anie.201911600, which has been published in final form at https://doi.org/10. 1002/anie.201911600. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.[EN] Prolonged (weeks) UV/Vis irradiation under Ar of UiO-66(Zr), UiO66 Zr-NO2, MIL101 Fe, MIL125 Ti-NH2, MIL101 Cr and MIL101 Cr(Pt) shows that these MOFs undergo photodecarboxylation of benzenedicarboxylate (BDC) linker in a significant percentage depending on the structure and composition of the material. Routine characterization techniques such as XRD, UV/Vis spectroscopy and TGA fail to detect changes in the material, although porosity and surface area change upon irradiation of powders. In contrast to BCD-containing MOFs, zeolitic imidazolate ZIF-8 does not evolve CO2 or any other gas upon irradiation.Financial support by the Spanish Ministry of Economy and Competitiveness (Severo Ochoa, and CTQ2015-69563-CO2-R1) and by the Generalitat Valenciana (Prometeo 2013-014) is gratefully acknowledged. J.A. thanks the Universitat Politecnica de Valencia for a postdoctoral scholarship. D.M. also thanks Spanish Ministry of Science for PhD Scholarship.Mateo-Mateo, D.; Santiago-Portillo, A.; Albero-Sancho, J.; Navalón Oltra, S.; Alvaro Rodríguez, MM.; García Gómez, H. (2019). Long-Term Photostability in Terephthalate Metal-Organic Frameworks. Angewandte Chemie International Edition. 58(49):17843-17848. https://doi.org/10.1002/anie.201911600S1784317848584

A proof-of-concept clinical trial using mesenchymal stem cells for the treatment of corneal epithelial stem cell deficiency

Producción CientíficaOcular stem cell transplantation derived from either autologous or allogeneic donor corneoscleral junction is a functional cell therapy to manage extensive and/or severe limbal stem cell deficiencies that lead to corneal epithelial failure. Mesenchymal stem cells have been properly tested in animal models of this ophthalmic pathology, but never in human eyes despite their potential advantages. We conducted a 6- to 12-month proof-of-concept, randomized, and double-masked pilot trial to test whether allogeneic bone marrow-derived mesenchymal stem cell transplantation (MSCT], n = 17) was as safe and as equally efficient as allogeneic cultivated limbal epithelial transplantation (CLET), (n = 11) to improve corneal epithelial damage due to limbal stem cell deficiency. Primary endpoints demanded combination of symptoms, signs, and the objective improvement of the epithelial phenotype in central cornea by in vivo confocal microscopy. This proof-of-concept trial showed that MSCT was as safe and efficacious as CLET. Global success at 6–12 months was 72.7%–77.8% for CLET cases and 76.5%–85.7% for MSCT cases (not significant differences). Central corneal epithelial phenotype improved in 71.4% and 66.7% of MSCT and CLET cases, respectively at 12 months (P = 1.000). There were no adverse events related to cell products. This trial suggests first evidence that MSCT facilitated improvement of a diseased corneal epithelium due to lack of its stem cells as efficiently as CLET. Consequently, not only CLET but also MSCT deserves more preclinical investigational resources before the favorable results of this proof-of-concept trial could be transformed into the larger numbers of the multicenter trials that would provide stronger evidence. (ClinicalTrials.gov number, NCT01562002.)Ministerio de Sanidad, Consumo y Bienestar Social (project SAS/2481/2009)Centro en Red de Medicina Regenerativa y Terapia Celular de Castilla y León (grant SAN 1178/200)Red de Terapia Celular TerCel (project RD12/0019/0036

Gas-Phase Photochemical Overall H2 S Splitting by UV Light Irradiation

[EN] Splitting of hydrogen sulfide is achieved to produce valueadded chemicals. Upon irradiation at 254 nm in the gas phase and in the absence of catalysts or photocatalysts at near room temperature, H2S splits into stoichiometric amounts of H2 and S with a quantum efficiency close to 50%. No influence of the presence of CH4 and CO2 (typical components in natural gas and biogas in which H2S is an unwanted component) on the efficiency of overall H2S splitting was observed. A mechanism for the H2 and S formation is proposed.Financial support by the Spanish Ministry of Economy and R1) and Generalitat Valenciana (Prometeo 2013-014) is gratefully acknowledged. Thanks are due to Dr. J. A. Agullo-Macia for performing a preliminary experiment.Garcia-Baldovi, H.; Albero-Sancho, J.; Ferrer Ribera, RB.; Mateo-Mateo, D.; Alvaro Rodríguez, MM.; García Gómez, H. (2017). Gas-Phase Photochemical Overall H2 S Splitting by UV Light Irradiation. ChemSusChem. 10(9):1996-2000. https://doi.org/10.1002/cssc.201700294S1996200010