Plants Probiotics as a Tool to Produce Highly Functional Fruits: The Case of Phyllobacterium and Vitamin C in Strawberries

10 páginas, 1 tabla, 1 figuraThe increasing interest in the preservation of the environment and the health of consumers is changing production methods and food consumption habits. Functional foods are increasingly demanded by consumers because they contain bioactive compounds involved in health protection. In this sense biofertilization using plant probiotics is a reliable alternative to the use of chemical fertilizers, but there are few studies about the effects of plant probiotics on the yield of functional fruits and, especially, on the content of bioactive compounds. In the present work we reported that a strain of genus Phyllobacterium able to produce biofilms and to colonize strawberry roots is able to increase the yield and quality of strawberry plants. In addition, the fruits from plants inoculated with this strain have significantly higher content in vitamin C, one of the most interesting bioactive compounds in strawberries. Therefore the use of selected plant probiotics benefits the environment and human health without agronomical losses, allowing the production of highly functional foods.This work was granted by “Junta de Castilla y León” (Regional Government, Grant SA183A11-2) and MINECO (Central Government, Grant AGL2011-29227). Paula García-Fraile is recipient of a postdoctoral researcher contract from Academy of Sciences of the Czech Republic. José David Flores-Félix was supported by a fellowship of Salamanca University. Marta Marcos García was supported by a fellowship of Fundación Miguel Casado San José. Luís R. Silva is grateful to the financial support from the European Union (FEDER funds through COMPETE) and National Funds (FCT, Fundação para a Ciência e Tecnologia) through project Pest-C/EQB/LA0006/2013 and from the European Union (FEDER funds) under the framework of QREN through Project NORTE-07-0124-FEDER- 000069.Peer reviewe

Diseño de un modelo de empresa, basado en principios de economía social, solidaria y colaborativa, para la recolección, tratamiento y disposición de productos derivados del caucho

En el municipio de San Miguel se presenta un problema en el manejo de desechos sólidos. Uno de los desechos más nocivos debido a su largo tiempo de degradación son los neumáticos fuera de uso; por tanto, se presenta una oportunidad de implementar un sistema que recolecte dichos neumáticos, los procese y distribuya el producto obtenido. Al implementar este proyecto bajo los principios de la Economía Social Solidaria y Colaborativa se contribuiría no solo a disminuir la acumulación de neumáticos fuera de uso junto con sus consecuencias a la salud y medio ambiente; sino también, a generar empleos a personas que están en riesgo de exclusión socia

Cortisol y estrés-recuperación durante un periodo competitivo enjugadores de balonman

with the perception of recovery-stress balance in handball players during a training and competition period. Fourteen handball playerstook part in the study during a major competition. Data were collected from six blood samples in order to measure cortisol, and from four applicationsof the RESTQ-Sport for stress-recovery balance assessment. Compared to pre-competition (243.81 ± 36.46 ng/mL), a significative decrease (p< .01) incortisol levels was observed in post-competition (162.22 ± 69.83 ng/mL). The RESTQ-Sport subscales general stress, emotional stress, fatigue, physicalrecovery, sleep quality, and disturbed breaks showed significant changes between pre and post-competition. Cortisol levels were found to have positive correlation with success subscale (rho= .53; p< .05), whilst negative with fitness/injury subscale (rho= -.57; p< .05). Our findings support the idea ofan anticipatory cortisol response to competition. Moreover, they provide further evidence on the influence of training over recovery-stress perception,emphasizing the importance of the psychophysiological and hormonal monitoring in order to achieve optimal athletic performance

Estrategias docentes alternativas empleando Youtube, Facebook y Twitter como herramientas dinamizadoras en el proceso de la enseñanza de la microbiología

Memoria ID-0196. Ayudas de la Universidad de Salamanca para la innovación docente, curso 2016-2017

ERK5 Is a major determinant of chemical sarcomagenesis: implications in human pathology

Sarcomas are a heterogeneous group of tumors in which the role of ERK5 is poorly studied. To clarify the role of this MAPK in sarcomatous pathology, we used a murine 3-methyl-cholanthrene (3MC)-induced sarcoma model. Our data show that 3MC induces pleomorphic sarcomas with muscle differentiation, showing an increased expression of ERK5. Indeed, this upregulation was also observed in human sarcomas of muscular origin, such as leiomyosarcoma or rhabdomyosarcoma. Moreover, in cell lines derived from these 3MC-induced tumors, abrogation of Mapk7 expression by using specific shRNAs decreased in vitro growth and colony-forming capacity and led to a marked loss of tumor growth in vivo. In fact, transcriptomic profiling in ERK5 abrogated cell lines by RNAseq showed a deregulated gene expression pattern for key biological processes such as angiogenesis, migration, motility, etc., correlating with a better prognostic in human pathology. Finally, among the various differentially expressed genes, Klf2 is a key mediator of the biological effects of ERK5 as indicated by its specific interference, demonstrating that the ERK5–KLF2 axis is an important determinant of sarcoma biology that should be further studied in human pathology.This work has been supported with Grant RTI2018-094093-B-I00 funded by MCIN/AEI/10.13039/501100011033, “ERDF A way of making Europe” to RSP. Also supported with funds from Fundación Leticia Castillejo Castillo, Roche España and ACEPAIN to RSP and MJRH. RSP and MJRH’s Research Institute and the work carried out in their laboratory, received partial support from the European Community through the FEDER. JJ and EAL hold a predoctoral research contract cofounded by the European Social Fund and UCLM. OR holds a contract for accessing the Spanish System of Science, Technology, and Innovation (SECTI) funded by the University of Castilla-La Mancha (UCLM) and received partial support from the European Social Fund (FSE) through its Operative Program for Castilla-La Mancha (2007–2013)

ERK5 Is a Major Determinant of Chemical Sarcomagenesis : Implications in Human Pathology

Sarcoma is a heterogeneous group of tumors poorly studied with few therapeutic opportunities. Interestingly, the role of MAPKs still remains unclear in sarcomatous pathology. Here, we describe for the first time the critical role of ERK5 in the biology of soft tissue sarcoma by using in vitro and in vivo approaches in a murine experimental model of chemical sarcomagenesis. Indeed, our observations were extrapolated to a short series of human leiomyosarcoma and rhabdomyosarcomas. Furthermore, transcriptome analysis allows us to demonstrate the critical role of KLF2 in the biological effects of ERK5. Therefore, the data presented here open new windows in the diagnosis and therapy of soft tissue sarcomas. Sarcomas are a heterogeneous group of tumors in which the role of ERK5 is poorly studied. To clarify the role of this MAPK in sarcomatous pathology, we used a murine 3-methyl-cholanthrene (3MC)-induced sarcoma model. Our data show that 3MC induces pleomorphic sarcomas with muscle differentiation, showing an increased expression of ERK5. Indeed, this upregulation was also observed in human sarcomas of muscular origin, such as leiomyosarcoma or rhabdomyosarcoma. Moreover, in cell lines derived from these 3MC-induced tumors, abrogation of Mapk7 expression by using specific shRNAs decreased in vitro growth and colony-forming capacity and led to a marked loss of tumor growth in vivo. In fact, transcriptomic profiling in ERK5 abrogated cell lines by RNAseq showed a deregulated gene expression pattern for key biological processes such as angiogenesis, migration, motility, etc., correlating with a better prognostic in human pathology. Finally, among the various differentially expressed genes, Klf2 is a key mediator of the biological effects of ERK5 as indicated by its specific interference, demonstrating that the ERK5-KLF2 axis is an important determinant of sarcoma biology that should be further studied in human pathology

Long-term follow-up of certolizumab pegol in uveitis due to immune-mediated inflammatory diseases : multicentre study of 80 patients

Objectives To evaluate effectiveness and safety of certolizumab pegol (CZP) in uveitis due to immune-mediated inflammatory diseases (IMID). Methods Multicentre study of CZP-treated patients with IMID uveitis refractory to conventional immunosuppressant. Effectiveness was assessed through the following ocular parameters: best-corrected visual acuity, anterior chamber cells, vitritis, macular thickness and retinal vasculitis. These variables were compared between the baseline, and first week, first, third, sixth months, first and second year. Results We studied 80 (33 men/47 women) patients (111 affected eyes) with a mean age of 41.6±11.7 years. The IMID included were: spondyloarthritis (n=43), Behçet's disease (n=10), psoriatic arthritis (n=8), Crohn's disease (n=4), sarcoidosis (n=2), juvenile idiopathic arthritis (n=1), reactive arthritis (n=1), rheumatoid arthritis (n=1), relapsing polychondritis (n=1), Conclusions CZP seems to be effective and safe in uveitis related to different IMID, even in patients refractory to previous biological drugs

Reorganización de los centros de capacitación de la Asociación Fe y Alegría tomo I Diagnóstico

La Asociación Fé y Alegria cuenta con centros de capacitación y cada uno de ellos tiene como objetivo primordial la reorganización de dichos centros, que consiste en el rediseño de los planes de estudios de los sistemas de control administrativo

Implementation of hydroponic crops as a pedagogical strategy for the promotion of research

En el presente proyecto se desarrollaron actividades pedagógicas para el fortalecimiento de los procesos educativos en la población estudiantil de la institución: Centro de Educación Básica San Valentín, en el departamento del Magdalena, a través de implementación de cultivos hidropónicos, como estrategias pedagógicas que desarrollan habilidades investigativas en infantes y jóvenes de la institución. Se trabajó bajo la metodología cualitativa con modelo de investigación acción participativa. La población estuvo conformada por estudiantes de esa institución. Se recogió la información por medio de las técnicas como relatorías individuales, institucionales, diario de campo, encuestas y observación directa. Se identificaron los contenidos curriculares del área de Biología para aplicar en los cultivos como un recurso generador de aprendizaje, con base en los cuales se realizó la planeación para las clases y el trabajo en los cultivos.In the present project, pedagogical activities were developed for the strengthening of educational processes in the student population of the San Valentín Basic Education Center, in the department of Magdalena, through the implementation of hydroponic crops as pedagogical strategies that develop investigative skills in infants and young people of the institution. We worked under the qualitative methodology with participatory action research model. The population was conformed by students of the San Valentín Basic Education Center, in Magdalena. The information was collected by means of techniques such as individual and institutional rapporteurships, field journals, surveys and direct observation. The curricular contents of the Biology area to be applied in the crops were identified as a learning instrument, based on which the planning for the classes and the work in the crops was carried out

Natural History of MYH7-Related Dilated Cardiomyopathy

BACKGROUND Variants in myosin heavy chain 7 (MYH7) are responsible for disease in 1% to 5% of patients with dilated cardiomyopathy (DCM); however, the clinical characteristics and natural history of MYH7-related DCM are poorly described. OBJECTIVES We sought to determine the phenotype and prognosis of MYH7-related DCM. We also evaluated the influence of variant location on phenotypic expression. METHODS We studied clinical data from 147 individuals with DCM-causing MYH7 variants (47.6% female; 35.6 +/- 19.2 years) recruited from 29 international centers. RESULTS At initial evaluation, 106 (72.1%) patients had DCM (left ventricular ejection fraction: 34.5% +/- 11.7%). Median follow-up was 4.5 years (IQR: 1.7-8.0 years), and 23.7% of carriers who were initially phenotype-negative developed DCM. Phenotypic expression by 40 and 60 years was 46% and 88%, respectively, with 18 patients (16%) first diagnosed at <18 years of age. Thirty-six percent of patients with DCM met imaging criteria for LV noncompaction. During follow-up, 28% showed left ventricular reverse remodeling. Incidence of adverse cardiac events among patients with DCM at 5 years was 11.6%, with 5 (4.6%) deaths caused by end-stage heart failure (ESHF) and 5 patients (4.6%) requiring heart transplantation. The major ventricular arrhythmia rate was low (1.0% and 2.1% at 5 years in patients with DCM and in those with LVEF of <= 35%, respectively). ESHF and major ventricular arrhythmia were significantly lower compared with LMNA-related DCM and similar to DCM caused by TTN truncating variants. CONCLUSIONS MYH7-related DCM is characterized by early age of onset, high phenotypic expression, low left ventricular reverse remodeling, and frequent progression to ESHF. Heart failure complications predominate over ventricular arrhythmias, which are rare. (C) 2022 The Authors. Published by Elsevier on behalf of the American College of Cardiology Foundation