Testing of Great Bay Oysters for Two Protozoan Pathogens

Two protozoan pathogens, Haplosporidium nelsoni (MSX) and Perkinsus marinus (Dermo), are known to be present in Great Bay oysters. With funds provided by the Piscataqua Region Estuaries Partnership ( PREP ), the Marine Fisheries Division of the New Ham pshire Fish and Game Department (NHF&G) continues to assess the presence and intensity of both of these disease conditions in oysters from the major beds within the Great Bay estuarine system. Histological examination s of Great Bay oysters have also revea led other endoparasites

Testing of Great Bay Oysters for Two Protozoan Pathogens

Two protozoan pathogens, Haplosporidium nelsoni (MSX) and Perkinsus marinus (Dermo), are known to be present in Great Bay oysters. With funds provided by the Piscataqua Region Estuaries Partnership (PREP), the Marine Fisheries Division of the New Hampshire Fish and Game Department (NHF&G) continues to assess the presence and intensity of both of these disease conditions in oysters from the major beds within the Great Bay estuarine system. Histological examinations of Great Bay oysters have also revealed other endoparasites

Traffic placement policies for a multi-band network

Recently protocols were introduced that enable the integration of synchronous traffic (voice or video) and asynchronous traffic (data) and extend the size of local area networks without loss in speed or capacity. One of these is DRAMA, a multiband protocol based on broadband technology. It provides dynamic allocation of bandwidth among clusters of nodes in the total network. A number of traffic placement policies for such networks are proposed and evaluated. Metrics used for performance evaluation include average network access delay, degree of fairness of access among the nodes, and network throughput. The feasibility of the DRAMA protocol is established through simulation studies. DRAMA provides effective integration of synchronous and asychronous traffic due to its ability to separate traffic types. Under the suggested traffic placement policies, the DRAMA protocol is shown to handle diverse loads, mixes of traffic types, and numbers of nodes, as well as modifications to the network structure and momentary traffic overloads

Development of Decision Trees for the Selection of Pavement Maintenance and Rehabilitation Activities in South-Central United States

Over time, new pavements deteriorate under the combined effects of traffic loading and the environment, no matter how well-designed or constructed. In general, maintenance and rehabilitation activities are employed to slow down or reset the rate of pavement deterioration. Cement-Stabilized Full Depth Reclamation (CSFDR) is a common rehabilitation treatment used by transportation agencies, specifically in Louisiana. Likewise, Ultra-Thin overlay (UTO) is a pavement maintenance treatment that has increased in popularity in recent years in Region 6. Yet, several gaps exist in the literature regarding the long-term field performance and cost-effectiveness of these two treatments especially in hot and humid climates. Therefore, the key objectives of this study were to assess the immediate benefits and long-term field performance as well as the cost-effectiveness of these two treatments in Louisiana. To achieve these objectives, numerous CSFDR and UTO projects were identified from the Louisiana Department of Transportation (LaDOTD) Pavement Management System (PMS) database and analyzed in terms of alligator cracks, rutting, random cracks, and roughness over a monitoring period of up to 15 years. Results indicated that the performance of CSFDR is significantly affected by the pre-treatment pavement conditions, applied overlay thickness, and traffic. Results also indicated that CSFDR projects would usually fail due to the development of random cracks. This could be attributed to the development of shrinkage cracks, which is a common problem with cement stabilization in Louisiana. A regression model was developed to predict the service life of CSFDR based on project conditions. Results also showed that UTO considerably extended the Pavement Service Life (PSL) for all the distress indices. This extension varied based on the pre-treatment pavement conditions and traffic level. As such, a predictive model was developed, with reasonable accuracy, to predict the extension in PSL of UTO based on project conditions. The developed models in this project for CSFDR and UTO will help state agencies make effective decisions for the maintenance and rehabilitation of their pavements

Colorado River Basin Study Comments--Arizona Department of Game & Fish

Comments on the Colorado River Basin Study prepared by the the Western Water Policy Review Advisory Commission

Temporal regulation of expression of immediate early and second phase transcripts by endothelin-1 in cardiomyocytes

Background: Endothelin-1 stimulates Gq protein-coupled receptors to promote proliferation in dividing cells or hypertrophy in terminally differentiated cardiomyocytes. In cardiomyocytes, endothelin-1 rapidly (within minutes) stimulates protein kinase signaling, including extracellular-signal regulated kinases 1/2 (ERK1/2; though not ERK5), with phenotypic/physiological changes developing from approximately 12 h. Hypertrophy is associated with changes in mRNA/protein expression, presumably consequent to protein kinase signaling, but the connections between early, transient signaling events and developed hypertrophy are unknown. Results: Using microarrays, we defined the early transcriptional responses of neonatal rat cardiomyocytes to endothelin-1 over 4 h, differentiating between immediate early gene (IEG) and second phase RNAs with cycloheximide. IEGs exhibited differential temporal and transient regulation, with expression of second phase RNAs within 1 h. Of transcripts upregulated at 30 minutes encoding established proteins, 28 were inhibited >50% by U0126 (which inhibits ERK1/2/5 signaling), with 9 inhibited 25-50%. Expression of only four transcripts was not inhibited. At 1 h, most RNAs (approximately 67%) were equally changed in total and polysomal RNA with approximately 17% of transcripts increased to a greater extent in polysomes. Thus, changes in expression of most protein-coding RNAs should be reflected in protein synthesis. However, approximately 16% of transcripts were essentially excluded from the polysomes, including some protein-coding mRNAs, presumably inefficiently translated. Conclusion: The phasic, temporal regulation of early transcriptional responses induced by endothelin-1 in cardiomyocytes indicates that, even in terminally differentiated cells, signals are propagated beyond the primary signaling pathways through transcriptional networks leading to phenotypic changes (that is, hypertrophy). Furthermore, ERK1/2 signaling plays a major role in this response

Exploring user motor behaviour in bimanual interactive video games

Video games have proved very valuable in rehabilitation technologies. They guide therapy and keep patients engaged and motivated. However, in order to realize their full potential, a good understanding is required of the players' motor control. In particular, little is known regarding player behaviour in tasks demanding bimanual interaction. In this work, an experiment was designed to improve the understanding of such tasks. A driving game was developed in which players were asked to guide a differential wheeled robot (depicted as a rocket) along a trajectory. The rocket could be manipulated by using an Xbox controller's triggers, each supplying torque to the corresponding side of the robot. Such a task is redundant, i.e. there exists an infinite number of input combinations to yield a given outcome. This allows the player to strategize according to their own preference. 10 participants were recruited to play this game and their input data was logged for subsequent analysis. Two different motor strategies were identified: an "intermittent" input pattern versus a "continuous" one. It is hypothesized that the choice of behaviour depends on motor skill and minimization of effort and error. Further testing is necessary to determine the exact relationship between these aspects

Linezolid therapy for infective endocarditis

AbstractLinezolid is not yet recognised as a standard therapy for infective endocarditis. This report describes nine patients with endocarditis treated with linezolid and 33 similar cases from the medical literature. The majority of cases involved multiresistant strains, and the reasons for administering linezolid were refractory disease (60%), intolerance (28%), sequential therapy (12%) and a resistant pathogen (1%). Linezolid was administered for a mean of 37 days, with a successful outcome in 79% of cases. Reversible adverse effects were described in ten cases. The mean follow-up period was 8.5 months. Further data from randomised controlled clinical trials are needed to determine the efficacy and safety of linezolid for treating endocarditis

Crowdfunding biodiversity conservation

Raising funds is critical for conserving biodiversity and hence so too is scrutinizing emerging financial mechanisms that might help achieve this goal. In this context, anecdotal evidence indicates crowdfunding is being used to support a variety of activities needed for biodiversity conservation, yet its magnitude and allocation remain largely unknown. We conducted a global analysis to help address this knowledge gap, based on empirical data from conservation‐focused projects extracted from crowdfunding platforms. For each project, we determined the funds raised, date, country of implementation, proponent characteristics, activity type, biodiversity realm, and target taxa. We identified 72 relevant platforms and 577 conservation‐focused projects that have raised US$4 790 634 since 2009. Whilst proponents were based in 38 countries, projects were delivered across 80 countries, indicating a potential mechanism of resource mobilization. Proponents were from non‐governmental organizations (35%), universities (30%), or were freelancers (26%). Most projects were for research (40%), persuasion (31%), and on‐ground actions (21%). Projects have focused primarily on species (57.7%) and terrestrial ecosystems (20.3%), and less on marine (8.8%) and freshwater ecosystems (3.6%). Projects have focused on 208 species, including a disproportionate number of threatened bird and mammal species. Crowdfunding for biodiversity conservation has now become a global phenomenon and presents signals for potential expansion, despite possible pitfalls. Opportunities arise from its spatial amplifying effect, steady increase over time, inclusion of Cinderella species, adoption by multiple actors, and funding of a range of activities beyond research. Our study paves the way for further research on key questions, such as campaign success rates, effectiveness, and drivers of adoption. Even though the capital input of crowdfunding so far has been modest compared to other conservation finance mechanisms, its contribution goes beyond funding research and providing capital. Embraced with due care, crowdfunding could potentially become an increasingly important financial mechanism for biodiversity conservation