Photometric observations of comet 81P/Wild 2 during the 2010 perihelion passage

Context. The Jupiter-family comet 81P/Wild 2, target of the NASA Stardust mission, is very important in the context of the studies of pristine objects in the solar system. First, it was only recently deflected into the present orbit, having spent at least 300 yr at higher heliocentric distance prior to the orbital change in 1974. It is therefore likely that the comet experienced a recent activation with consequent low alteration of its original material. Second, it is the only comet whose coma material was brought back to Earth for laboratory analysis. We observed the object between 2010 February 9 and September 9 for a total of 11 nights during the 2010 perihelion passage. Aims. The goals of the campaign were the characterization of the comet's dust activity and the comparison with previous apparitions to derive hints on the secular behavior of the object. Methods. Broadband R-and I-images were acquired using three instruments: ALFOSC, CAMELOT, and TCP. The first one is mounted at the Nordic Optical Telescope on La Palma, while the second and the third are mounted at the Instituto de Astrofisica de Canarias 0.82-m telescope on Tenerife. We analyzed the presence and variability of dust structures in the coma with image-enhancing techniques, the radial profile of the dust brightness, and we measured the dust production rate and the dust reddening. Results. We found evidence of a long-lasting sunward fan and anti-solar tail activity throughout all our observations up to a heliocentric distance of 2.42 AU. Afρ measurements suggest a pre-perihelion peak of the activity, caused by a seasonal effect, plus two post-perihelion outbursts. Both spatial and Afρ radial profiles indicate a steady-state coma at nucleocentric distances greater than ~1000-2000 km. The color analysis reveals a moderately reddened dust with a 6-9%/1000 reddening, consistent with the current picture of cometary dust. The second outburst emitted dust with lower reddening. Conclusions. The comparison with previous perihelion passages points toward a recurrent main activity always driven by the same areas on the nucleus, producing dust with similar characteristics and in similar coma structures in different years. Our Afρ measurement at the longest heliocentric distance suggests the comet was less dust-productive in 2010, pointing toward a possible secular aging of the object and its activity. The change of dust colors during the unusual second outburst suggests that an internal part of the nucleus has different physical properties compared with those that produce the recurrent main activity, pointing toward a heterogeneous comet. © ESO, 2012

Instability of small-amplitude convective flows in a rotating layer with stress-free boundaries

We consider stability of steady convective flows in a horizontal layer with stress-free boundaries, heated below and rotating about the vertical axis, in the Boussinesq approximation (the Rayleigh-Benard convection). The flows under consideration are convective rolls or square cells, the latter being asymptotically equal to the sum of two orthogonal rolls of the same wave number k. We assume, that the Rayleigh number R is close to the critical one, R_c(k), for the onset of convective flows of this wave number: R=R_c(k)+epsilon^2; the amplitude of the flows is of the order of epsilon. We show that the flows are always unstable to perturbations, which are a sum of a large-scale mode not involving small scales, and two large-scale modes, modulated by the original rolls rotated by equal small angles in the opposite directions. The maximal growth rate of the instability is of the order of max(epsilon^{8/5},(k-k_c)^2), where k_c is the critical wave number for the onset of convection.Comment: Latex, 12 pp., 15 refs. An improved version of the manuscript submitted to "Mechanics of fluid and gas", 2006 (in Russian; English translation "Fluid Dynamics"

Pre-hibernation performances of the OSIRIS cameras onboard the Rosetta spacecraft

Context. The ESA cometary mission Rosetta was launched in 2004. In the past years and until the spacecraft hibernation in June 2011, the two cameras of the OSIRIS imaging system (Narrow Angle and Wide Angle Camera, NAC and WAC) observed many different sources. On 20 January 2014 the spacecraft successfully exited hibernation to start observing the primary scientific target of the mission, comet 67P/Churyumov-Gerasimenko. Aims. A study of the past performances of the cameras is now mandatory to be able to determine whether the system has been stable through the time and to derive, if necessary, additional analysis methods for the future precise calibration of the cometary data. Methods. The instrumental responses and filter passbands were used to estimate the efficiency of the system. A comparison with acquired images of specific calibration stars was made, and a refined photometric calibration was computed, both for the absolute flux and for the reflectivity of small bodies of the solar system. Results. We found a stability of the instrumental performances within ±1.5% from 2007 to 2010, with no evidence of an aging effect on the optics or detectors. The efficiency of the instrumentation is found to be as expected in the visible range, but lower than expected in the UV and IR range. A photometric calibration implementation was discussed for the two cameras. Conclusions. The calibration derived from pre-hibernation phases of the mission will be checked as soon as possible after the awakening of OSIRIS and will be continuously monitored until the end of the mission in December 2015. A list of additional calibration sources has been determined that are to be observed during the forthcoming phases of the mission to ensure a better coverage across the wavelength range of the cameras and to study the possible dust contamination of the optics

Epstein-Barr virus transcription factor Zta acts through distal regulatory elements to directly control cellular gene expression

Lytic replication of the human gamma herpes virus Epstein-Barr virus (EBV) is an essential prerequisite for the spread of the virus. Differential regulation of a limited number of cellular genes has been reported in B-cells during the viral lytic replication cycle. We asked whether a viral bZIP transcription factor, Zta (BZLF1, ZEBRA, EB1), drives some of these changes. Using genome-wide chromatin immunoprecipitation coupled to next-generation DNA sequencing (ChIP-seq) we established a map of Zta interactions across the human genome. Using sensitive transcriptome analyses we identified 2263 cellular genes whose expression is significantly changed during the EBV lytic replication cycle. Zta binds 278 of the regulated genes and the distribution of binding sites shows that Zta binds mostly to sites that are distal to transcription start sites. This differs from the prevailing view that Zta activates viral genes by binding exclusively at promoter elements. We show that a synthetic Zta binding element confers Zta regulation at a distance and that distal Zta binding sites from cellular genes can confer Zta-mediated regulation on a heterologous promoter. This leads us to propose that Zta directly reprograms the expression of cellular genes through distal elements

Optimality of mutation and selection in germinal centers

The population dynamics theory of B cells in a typical germinal center could play an important role in revealing how affinity maturation is achieved. However, the existing models encountered some conflicts with experiments. To resolve these conflicts, we present a coarse-grained model to calculate the B cell population development in affinity maturation, which allows a comprehensive analysis of its parameter space to look for optimal values of mutation rate, selection strength, and initial antibody-antigen binding level that maximize the affinity improvement. With these optimized parameters, the model is compatible with the experimental observations such as the ~100-fold affinity improvements, the number of mutations, the hypermutation rate, and the "all or none" phenomenon. Moreover, we study the reasons behind the optimal parameters. The optimal mutation rate, in agreement with the hypermutation rate in vivo, results from a tradeoff between accumulating enough beneficial mutations and avoiding too many deleterious or lethal mutations. The optimal selection strength evolves as a balance between the need for affinity improvement and the requirement to pass the population bottleneck. These findings point to the conclusion that germinal centers have been optimized by evolution to generate strong affinity antibodies effectively and rapidly. In addition, we study the enhancement of affinity improvement due to B cell migration between germinal centers. These results could enhance our understandings to the functions of germinal centers.Comment: 5 figures in main text, and 4 figures in Supplementary Informatio

Commentary on the WHO classification of tumors of lymphoid tissues (2008): aggressive B-cell lymphomas

In the novel WHO classification 2008, the classification of aggressive B-cell lymphoma has been revised for several categories with the aim to define “clean” entities. Within large B-cell lymphoma, a few distinct clinico-pathological entities have been recognized with more clinically defined entities than pathologically defined ones. The majority of known morphological variations were not considered to merit more than classification as a variant of DLBCL, not otherwise specified. Specifically, a biological subgrouping of DLBCL on the basis of molecular (activated B-cell versus germinal center B-cell) or immunophenotypic (CD5+) features was felt to be too immature to include at this stage. The role of EBV in aggressive B-cell lymphoma has been explored in more depth with the recognition of several novel and re-defined clinico-pathological entities. Also, in these diseases, clinical definitions play a very dominant role in the WHO classification 2008

Enabling the classroom and the curriculum: higher education, literary studies and disability

In this article the tripartite model of disability is applied to the lived experience of twenty-first-century higher education. The tripartite model facilitates a complex understanding of disability that recognises assumptions and discrimination but not at the cost of valued identity. This being so, not only the normative positivisms and non-normative negativisms but also the non-normative positivisms of the classroom and the curriculum are explored. Inclusion is taken as the starting point and the argument progresses to a profound and innovational appreciation of disability. The problem addressed is that inclusion, as shown in The Biopolitics of Disability, constitutes little more than inclusion-ism until disability is recognised in the context of alternative lives and values that neither enforce nor reify normalcy. Informed by this understanding, the article adopts the disciplinary example of literary studies and refers to Brian Friel’s Molly Sweeney as a primary text. The conclusion is that, despite passive and active resistance, disability enters higher education in many ways, most of which are beneficial to students and educators alike

Induction of interferon-stimulated genes on the IL-4 response axis by Epstein-Barr virus infected human b cells; relevance to cellular transformation.

Epstein-Barr virus (EBV) is an oncogenic virus that is associated with the pathogenesis of several human lymphoid malignancies, including Hodgkin's lymphoma. Infection of normal resting B cells with EBV results in activation to lymphoblasts that are phenotypically similar to those generated by physiological stimulation with CD40L plus IL-4. One important difference is that infection leads to the establishment of permanently growing lymphoblastoid cell lines, whereas CD40L/IL-4 blasts have finite proliferation lifespans. To identify early events which might later determine why EBV infected blasts go on to establish transformed cell lines, we performed global transcriptome analyses on resting B cells and on EBV and CD40L/IL-4 blasts after 7 days culture. As anticipated there was considerable overlap in the transcriptomes of the two types of lymphoblasts when compared to the original resting B cells, reflecting common changes associated with lymphocyte activation and proliferation. Of interest to us was a subset of 255 genes that were differentially expressed between EBV and CD40L/IL-4 blasts. Genes which were more highly expressed in EBV blasts were substantially and significantly enriched for a set of interferon-stimulated genes which on further in silico analyses were found to be repressed by IL-4 in other cell contexts and to be up-regulated in micro-dissected malignant cells from Hodgkin's lymphoma biopsies when compared to their normal germinal center cell counterparts. We hypothesized that EBV and IL-4 were targeting and discordantly regulating a common set of genes. This was supported experimentally in our B cell model where IL-4 stimulation partially reversed transcriptional changes which follow EBV infection and it impaired the efficiency of EBV-induced B cell transformation. Taken together, these data suggest that the discordant regulation of interferon and IL-4 pathway genes by EBV that occurs early following infection of B cells has relevance to the development or maintenance of an EBV-associated malignancy

EBV, HHV8 and HIV in B cell non Hodgkin lymphoma in Kampala, Uganda

<p>Abstract</p> <p>Background</p> <p>B cell non Hodgkin lymphomas account for the majority of lymphomas in Uganda. The commonest is endemic Burkitt lymphoma, followed by diffuse large-B-cell lymphoma (DLBCL). There has been an increase in incidence of malignant lymphoma since the onset of the HIV/AIDS pandemic. However, the possible linkages of HHV8 and EBV to the condition of impaired immunity present in AIDS are still not yet very clearly understood.</p> <p>Objectives</p> <p indent="1">1. To describe the prevalence of Epstein-Barr virus, Human Herpes virus 8 and Human Immunodeficiency Virus-1 in B cell non Hodgkin lymphoma biopsy specimens in Kampala, Uganda.</p> <p indent="1">2. To describe the histopathology of non Hodgkin lymphoma by HIV serology test result in Kampala, Uganda</p> <p>Method</p> <p>Tumour biopsies specimens from 119 patients with B cell non Hodgkin lymphoma were classified according to the WHO classification. Immunohistochemistry was used for detection of HHV8 and in situ hybridization with Epstein Barr virus encoded RNA (EBER) for EBV. Real time and nested PCR were used for the detection of HIV.</p> <p>The patients from whom the 1991-2000 NHL biopsies had been taken did not have HIV serology results therefore 145 patients biopsies where serology results were available were used to describe the association of HIV with non Hodgkin lymphoma type during 2008-2009.</p> <p>Results</p> <p>In this study, the majority (92%) of the Burkitt lymphomas and only 34.8% of the diffuse large B cell lymphomas were EBV positive. None of the precursor B lymphoblastic lymphomas or the mantle cell lymphomas showed EBV integration in the lymphoma cells.</p> <p>None of the Burkitt lymphoma biopsies had HIV by PCR. Of the 121 non Hodgkin B cell lymphoma patients with HIV test results, 19% had HIV. However, only 1(0.04%) case of Burkitt lymphoma had HIV. All the tumours were HHV8 negative.</p> <p>Conclusions</p> <p>The majority of the Burkitt lymphomas and two fifths of the diffuse large B cell lymphomas had EBV. All the tumours were HHV8 negative. Generally, the relationship of NHL and HIV was weaker than what has been reported from the developed countries. We discuss the role of these viruses in lymphomagenesis in light of current knowledge.</p

The dust environment of comet 67P/Churyumov-Gerasimenko from Rosetta OSIRIS and VLT observations in the 4.5 to 2.9 au heliocentric distance range inbound

Context. The ESA Rosetta spacecraft, currently orbiting around cornet 67P/Churyumov-Gerasimenko, has already provided in situ measurements of the dust grain properties from several instruments, particularly OSIRIS and GIADA. We propose adding value to those measurements by combining them with ground-based observations of the dust tail to monitor the overall, time-dependent dust-production rate and size distribution. Aims. To constrain the dust grain properties, we take Rosetta OSIRIS and GIADA results into account, and combine OSIRIS data during the approach phase (from late April to early June 2014) with a large data set of ground-based images that were acquired with the ESO Very Large Telescope (VLT) from February to November 2014. Methods. A Monte Carlo dust tail code, which has already been used to characterise the dust environments of several comets and active asteroids, has been applied to retrieve the dust parameters. Key properties of the grains (density, velocity, and size distribution) were obtained from. Rosetta observations: these parameters were used as input of the code to considerably reduce the number of free parameters. In this way, the overall dust mass-loss rate and its dependence on the heliocentric distance could be obtained accurately. Results. The dust parameters derived from the inner coma measurements by OSIRIS and GIADA and from distant imaging using VLT data are consistent, except for the power index of the size-distribution function, which is alpha = -3, instead of alpha = -2, for grains smaller than 1 mm. This is possibly linked to the presence of fluffy aggregates in the coma. The onset of cometary activity occurs at approximately 4.3 AU, with a dust production rate of 0.5 kg/s, increasing up to 15 kg/s at 2.9 AU. This implies a dust-to-gas mass ratio varying between 3.8 and 6.5 for the best-fit model when combined with water-production rates from the MIRO experiment