Mangrove microflora as potential source of hydrolytic enzymes for commercial applications

678-684The purpose of this study was to isolate and characterize mangrove microflora based on their hydrolytic enzyme production. A collection of 100 microorganisms including bacteria, actinomycetes, fungi and yeasts was isolated. The ability of microbial isolates to degrade hydrolytic enzymes such as amylase, cellulase, chitinase, glutaminase, laccase, ligninase, lipase, protease and tyrosinase were tested and the potent strains were identified based on 16S rRNA and ITS sequencing. More than 90% of the isolates exhibited amylolytic and proteolytic activity. Potent isolates were identified as: Bacillus subtilis (MB1), Bacillus amyloliquefaciens (MB11), Bacillus megaterium (MB23), Bacillus mojavensis (MB28), Streptomyces galbus (MA7), Streptomyces sp. (MA3), Candida parapsilopsis (MY6), Candida etchellsi (MY1), Penicillium citrinum (MF5), Aspergillus stellifer (MF12) and Emericella sp. (MF18). These microbes as well as the enzymes are of potential importance for commercial applications as bioremediators, detergent additives and nutritional supplements

Interventions and instruments to promote sustainable land use in Europe

Since the first examples of human settlements, land has been subject to transformations. With the industrial revolution and in particular after the Second World War, land transformation has become more intense in terms of overexploitation of natural resources. Only recently, however, have policy and decision makers acknowledged the importance of land as a finite resource. Sustainable development has increasingly become the subject of many studies and a reason for debate, leading to different lines of thought among the various disciplines. This has led to the design and implementation of a great number of sustainable land use practices in many European cities and regions. In fact, it seems that sustainability of land use depends both on the socio-economic processes that trigger spatial development and the effectiveness of the instruments that regulate these processes (Solly et al., 2020). The need for a more sustainable and eco-compatible approach, and the European objective to achieve zero net land take 2050 (Science for Environment Policy, 2016), have been one of the reasons why ESPON launched the pan-European research project SUPER – Sustainable Urbanization and land-use in the European Regions. The project examines, on the one hand, the nature and the structure of urbanization processes and, on the other, it seeks to analyze the degree of success of interventions and instruments aiming at a more sustainable use of land. This contribution presents the results of the project by focusing especially on the content of the SUPER “Guide to Sustainable Urbanisation and Land Use” (Cotella et al., 2020). Thanks to the exploration of more than two hundred examples of urbanization interventions gathered all over Europe, the guide suggests a set of policy recommendations for policy and decision makers in order to address land use in a more sustainable perspective. According to the different objectives and contextual needs, policy and decision makers should focus more on: (i) which intervention (i.e. densification, regeneration, containment, governance and sectoral policies) and (ii) which instrument, should be used (i.e. visions and strategies, rules and legal devices, land use regulations, programmes and projects)

ESPON SUPER – Sustainable Urbanisation and land-use Practices in European Regions. A GUIDE TO SUSTAINABLE URBANISATION AND LAND-USE

Guides help you do things. You turn to them when you need to find out how to solve a problem. They are a form of knowledge transfer, written by experts in a way that is accessible and helpful to a wide audience. This guide was written by the researchers engaged in the ESPON 2020 applied research project on Sustainable Urbanisation and Land-Use Practices in European Regions (SUPER). It aims to help people and institutions engaged with land-use management at various levels across Europe to promote sustainable urbanisation in their territories. Overall, the guide offers information, ideas and perspectives to help decision-makers and policymakers to proactively contribute to more equal, balanced, and sustainable territorial development. The decision to convert land to a different use influences our quality of life and that of future generations, and, as this Guide shows, a large toolbox of interventions exists that can help alter prevailing land-use practices. Choosing among them is a tough decision, and implementation may require strong political commitment and bold leadership. We hope that this Guide provides the inspiration to accept this challenge

The Design and Development of the SMEX-Lite Power System

This paper describes the design and development of a 250W orbit average electrical power system electronic Power Node and software for use in Low Earth Orbit missions. The mass of the Power Node is 3.6 Kg (8 lb.). The dimensions of the Power Node are 30cm x 26cm x 7.9cm (11 in. x 10.25 in x 3.1 in.) The design was realized using software, Field Programmable Gate Array (FPGA) digital logic and surface mount technology. The design is generic enough to reduce the non-recurring engineering for different mission configurations. The Power Node charges one to five, low cost, 22-cell 4 AH D-cell battery packs independently. The battery charging algorithms are executed in the power software to reduce the mass and size of the power electronic. The Power Node implements a peak-power tracking algorithm using an innovative hardware/software approach. The power software task is hosted on the spacecraft processor. The power software task generates a MIL-STD-1553 command packet to update the Power Node control settings. The settings for the battery voltage and current limits, as well as minimum solar array voltage used to implement peak power tracking are contained in this packet. Several advanced topologies are used in the Power Node. These include synchronous rectification in the bus regulators, average current control in the battery chargers and quasi-resonant converters for the Field Effect Transistor (FET) transistor drive electronics. Lastly, the main bus regulator uses a feed-forward topology with the PWM implemented in an FPGA

Analysis of the NGXO Telescope X-Ray Hartmann Data

Next Generation X-Ray Optics (NGXO) team at the Goddard Space Flight Center (GSFC) has been developing a new silicon-based grazing incidence mirror technology for future high resolution x-ray astronomical missions. Recently, the GSFC team completed the construction of first few mirror modules that contain one pair of mirrors. One of the mirror pairs was tested in GSFC 600-m long beamline facility and Panter (Neuried, Germay) 120-m long x-ray beamline facility. Both full aperture x-ray tests, Hartmann tests, and focal plane sweeps were completed. In this paper we present the data analysis process and compare the results from our models to measured x-ray centroid data, x-ray performance data, and out of focus images of the mirror pair

Challenges of Early Years leadership preparation: a comparison between early and experienced Early Years practitioners in England

Leadership has been under-researched in the Early Years (EY) sector of primary schools in England, especially in leading change for professional development. The aim of this paper is to theorise what the leadership culture for EY practitioners looks like, and how Initial Teacher Training providers and schools are preparing practitioners for leadership. Using case studies of EY practitioners in different stages of their career in primary schools, we offer an insight into their preparedness for leadership in EY, the implication being that leadership training requires an understanding and embedding of the EY culture and context. Interviews with both sample groups allowed for deeper insight into the lived world. Interviews were also conducted with the head teachers to gain an overview of the leadership preparation they provided. The main findings suggest that newer EY practitioners are better prepared for leadership from their university training in comparison to more experienced EY practitioners

Sigma-phase in Fe-Cr and Fe-V alloy systems and its physical properties

A review is presented on physical properties of the sigma-phase in Fe-Cr and Fe-V alloy systems as revealed both with experimental -- mostly with the Mossbauer spectroscopy -- and theoretical methods. In particular, the following questions relevant to the issue have been addressed: identification of sigma and determination of its structural properties, kinetics of alpha-to-sigma and sigma-to-alpha phase transformations, Debye temperature and Fe-partial phonon density of states, Curie temperature and magnetization, hyperfine fields, isomer shifts and electric field gradients.Comment: 26 pages, 23 figures and 83 reference

Which factors impact on quality of life for adults with blepharospasm and hemifacial spasm?

Purpose: Benign essential blepharospasm (BEB) and hemifacial spasm (HFS) are debilitating conditions causing spasms to the eyes and/or face and can significantly impact on quality of life (QoL). Initial research has highlighted potential factors impacting on QoL in BEB, but there remains a wealth of demographic, clinical, and psychosocial factors that may contribute to QoL but have not received attention. Methods: Cross-sectional baseline data were collected before a single-masked randomised controlled trial from 130 adults with BEB and HFS recruited from botulinum toxin clinics at Moorfields Eye Hospital, London. QoL was measured using the 24-item Craniocervical Dystonia Questionnaire (CDQ24), which provides a total score and five subscale scores relating to Stigma, Emotional state, Pain, Activities of daily living (ADL), and Social/family life. Treating clinicians provided clinical data. Hierarchical multiple regressions were performed on this baseline data to identify significant predictors of QoL. Results: ADL and Stigma were the areas most impacted upon whilst patients experienced better adjustment in relation to Pain, Social/family life, and Emotional state. CDQ24 Total scores were explained by the model (80% variance) and were significantly associated with appearance concerns, emotional representations, perceived negative consequences of the condition, mood, and dose of botulinum toxin. Conclusions: Patients with BEB and HFS report a detrimental impact on ADL and perceived stigma in relation to their condition. Predominantly, individual perceptions and mood are associated with QoL in this population, rather than demographic and clinical factors, signifying areas to target in the design of future healthcare services or interventions

Development of FRET Assay into Quantitative and High-throughput Screening Technology Platforms for Protein–Protein Interactions

Förster resonance energy transfer (FRET) technology has been widely used in biological and biomedical research and is a very powerful tool in elucidating protein interactions in many cellular processes. Ubiquitination and SUMOylation are multi-step cascade reactions, involving multiple enzymes and protein–protein interactions. Here we report the development of dissociation constant (Kd) determination for protein–protein interaction and cell-based high-throughput screening (HTS) assay in SUMOylation cascade using FRET technology. These developments are based on steady state and high efficiency of fluorescent energy transfer between CyPet and YPet fused with SUMO1 and Ubc9, respectively. The developments in theoretical and experimental procedures for protein interaction Kd determination and cell-based HTS provide novel tools in affinity measurement and protein interaction inhibitor screening. The Kd determined by FRET between SUMO1 and Ubc9 is compatible with those determined with other traditional approaches, such as isothermal titration calorimetry (ITC) and surface plasmon resonance (SPR). The FRET-based HTS is pioneer in cell-based HTS. Both Kd determination and cell-based HTS, carried out in 384-well plate format, provide powerful tools for large-scale and high-throughput applications

Land‐use intensity and biodiversity effects on infiltration capacity and hydraulic conductivity of grassland soils in southern Germany

Evidence from experimental and established grasslands indicates that plant biodiversity can modify the water cycle. One suspected mechanism behind this is a higher infiltration capacity (ν$_{B}$) and hydraulic conductivity (K) of the soil on species-rich grasslands. However, in established and agriculturally managed grasslands, biodiversity effects cannot be studied independent of land-use effects. Therefore, we investigated in established grassland systems how land-use intensity and associated biodiversity of plants and soil animals affect νB and K at and close to saturation. On 50 grassland plots along a land-use intensity gradient in the Biodiversity Exploratory Schwäbische Alb, Germany, we measured νB with a hood infiltrometer at several matrix potentials and calculated the saturated and unsaturated K. We statistically analysed the relationship between ν$_{B}$ or K and land-use information (e.g., fertilising intensity), abiotic (e.g., soil texture) and biotic data (e.g., plant species richness, earthworm abundance). Land-use intensity decreased and plant species richness increased ν$_{B}$ and K, while the direction of the effects of soil animals was inconsistent. The effect of land-use intensity on ν$_{B}$ and K was mainly attributable to its negative effect on plant species richness. Our results demonstrate that plant species richness was a better predictor of ν$_{B}$ and K at and close to saturation than land-use intensity or soil physical properties in the established grassland systems of the Schwäbische Alb