High-power, continuous-wave, scalable, single-frequency 852nm laser source for 213nm generation

We developed a high-power, continuous-wave (CW), single-frequency 852nm laser source, for the purpose of fourth harmonic generation at 213nm. Our approach is the doubly resonant sum-frequency mixing (DRSFM) with two fiber sources. An in-house single-frequency master oscillator at 1907nm is amplified by an in-house clad-pumped amplifier to 5W, and a commercial single-frequency master oscillator at 1540nm is amplified by a commercial amplifier to 10W. The two beams are combined via a dichroic mirror to a single beam before incident on a dual-wavelength resonator, consisting of one set of dual-wavelength mirrors. The external resonator is locked to the 1907nm laser frequency, and the frequency of the 1540nm is locked to the resonator, realizing double-resonance. With a periodically-poled stoichiometric lithium tantalate in the resonator, the sum-frequency at 852nm is efficiently generated. All 3 waves are in the same polarization (e-ray), allowing the effective use of Brewster-cut device, eliminating reflection loss for all wavelengths without any antireflection coatings. With 4.6W at 1907nm and 7.7W at 1540nm incident onto the resonator, 5.2W at 852nm was generated, representing the efficiency of greater than 40%. The experimental result indicates our current setup will be more efficient with higher input powers at 1907nm. With both fiber sources at 1540nm and 1907nm being scalable in output power, the output at 852nm is also scalable. By the forth harmonic of 852nm, 0.456 W CW 213nm was generated.New Energy and Industrial Technology Development Organization (NEDO) of JapanThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at [email protected]

Scalable Approach for Continuous-Wave Deep-Ultraviolet Laser at 213nm

We present a novel approach for generation at 213nm in continuous-wave, corresponding to the fifth harmonic of common 1064nm laser, in pure continuous-wave mode. The approach is scalable in output power. Starting from two infrared fiber laser sources, we demonstrated 0.456W output at 213nm. (C) 2019 Optical Society of America under the terms of the OSA Open Access Publishing AgreementOpen access journal.This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at [email protected]

Chain and mirophase-separated structures of ultrathin polyurethane films

Measurements are presented how chain and microphase-separated structures of ultrathin polyurethane (PU) films are controlled by the thickness. The film thickness is varied by a solution concentration for spin coating. The systems are PUs prepared from commercial raw materials. Fourier-transform infrared spectroscopic measurement revealed that the degree of hydrogen bonding among hard segment chains decreased and increased with decreasing film thickness for strong and weak microphase separation systems, respectively. The microphase-separated structure, which is formed from hard segment domains and a surrounding soft segment matrix, were observed by atomic force microscopy. The size of hard segment domains decreased with decreasing film thickness, and possibility of specific orientation of the hard segment chains was exhibited for both systems. These results are due to decreasing space for the formation of the microphase-separated structure.IUMRS-ICA 2008 Symposium Sessions X and Y. Soft Matter Science, 9-13 December, Nagoya, Japa

Structural basis of Sec-independent membrane protein insertion by YidC

[プレスリリース]バイオサイエンス研究科膜分子複合機能学研究室の塚崎智也准教授らの研究グループが、タンパク質を細胞膜に組み込むメカニズムを解明しました（2014/04/17）Newly synthesized membrane proteins must be accurately inserted into the membrane, folded and assembled for proper functioning. The protein YidC inserts its substrates into the membrane, thereby facilitating membrane protein assembly in bacteria; the homologous proteins Oxa1 and Alb3 have the same function in mitochondria and chloroplasts, respectively1, 2. In the bacterial cytoplasmic membrane, YidC functions as an independent insertase and a membrane chaperone in cooperation with the translocon SecYEG3, 4, 5. Here we present the crystal structure of YidC from Bacillus halodurans, at 2.4 Å resolution. The structure reveals a novel fold, in which five conserved transmembrane helices form a positively charged hydrophilic groove that is open towards both the lipid bilayer and the cytoplasm but closed on the extracellular side. Structure-based in vivo analyses reveal that a conserved arginine residue in the groove is important for the insertion of membrane proteins by YidC. We propose an insertion mechanism for single-spanning membrane proteins, in which the hydrophilic environment generated by the groove recruits the extracellular regions of substrates into the low-dielectric environment of the membrane

Correlation Index-Based Responsible-Enzyme Gene Screening (CIRES), a Novel DNA Microarray-Based Method for Enzyme Gene Involved in Glycan Biosynthesis

BACKGROUND: Glycan biosynthesis occurs though a multi-step process that requires a variety of enzymes ranging from glycosyltransferases to those involved in cytosolic sugar metabolism. In many cases, glycan biosynthesis follows a glycan-specific, linear pathway. As glycosyltransferases are generally regulated at the level of transcription, assessing the overall transcriptional profile for glycan biosynthesis genes seems warranted. However, a systematic approach for assessing the correlation between glycan expression and glycan-related gene expression has not been reported previously. METHODOLOGY: To facilitate genetic analysis of glycan biosynthesis, we sought to correlate the expression of genes involved in cell-surface glycan formation with the expression of the glycans, as detected by glycan-recognizing probes. We performed cross-sample comparisons of gene expression profiles using a newly developed, glycan-focused cDNA microarray. Cell-surface glycan expression profiles were obtained using flow cytometry of cells stained with plant lectins. Pearson's correlation coefficients were calculated for these profiles and were used to identify enzyme genes correlated with glycan biosynthesis. CONCLUSIONS: This method, designated correlation index-based responsible-enzyme gene screening (CIRES), successfully identified genes already known to be involved in the biosynthesis of certain glycans. Our evaluation of CIRES indicates that it is useful for identifying genes involved in the biosynthesis of glycan chains that can be probed with lectins using flow cytometry

ミズ カンキョウ オセン ブッシツ ノ ドウタイ ヒョウカ ケンキュウ キョテン ノ コウチク

本拠点形成では、地球規模での省資源･持続･循環社会の実現において中核となる環境科学の分野で画期的な貢献をするために、熊本大学の立地条件に配慮し、緊急性の高い水環境汚染物質の動態評価研究拠点の構築を目指す。とくに、環境悪化の原因とメカニズムが不明のまま疲弊状態にある有明･八代海の再生修復への貢献を念頭に置き、環境ホルモンなどの環境汚染物質の生物モニタリング科学創成、沿岸域における栄養塩除去などに有効な環境汚染物質のバイオ・レミディエーションの科学技術研究の展開、陸水と海水に跨る沿岸域水環境の計測･解析･評価手法の高度化と応用、微量で多様な環境汚染物質の高精度定量手法の開発と応用などの先導的研究を推進する。また、これらの研究を通じて総合的な研究能力と高度な洞察力を有する創造性豊かな人材を育成するとともに、環境産業創成に貢献する

ミズ カンキョウ オセン ブッシツ ノ ドウタイ ヒョウカ ケンキュウ キョテン ノ コウチク

本拠点形成では、地球規模での省資源･持続･循環社会の実現において中核となる環境科学の分野で画期的な貢献をするために、熊本大学の立地条件に配慮し、緊急性の高い水環境汚染物質の動態評価研究拠点の構築を目指す。とくに、環境悪化の原因とメカニズムが不明のまま疲弊状態にある有明･八代海の再生修復への貢献を念頭に置き、環境ホルモンなどの環境汚染物質の生物モニタリング科学創成、沿岸域における栄養塩除去などに有効な環境汚染物質のバイオ・レミディエーションの科学技術研究の展開、陸水と海水に跨る沿岸域水環境の計測･解析･評価手法の高度化と応用、微量で多様な環境汚染物質の高精度定量手法の開発と応用などの先導的研究を推進する。また、これらの研究を通じて総合的な研究能力と高度な洞察力を有する創造性豊かな人材を育成するとともに、環境産業創成に貢献する

ミズ カンキョウ オセン ブッシツ ノ ドウタイ ヒョウカ ケンキュウ キョテン ノ コウチク

本拠点形成では、地球規模での省資源･持続･循環社会の実現において中核となる環境科学の分野で画期的な貢献をするために、熊本大学の立地条件に配慮し、緊急性の高い水環境汚染物質の動態評価研究拠点の構築を目指す。とくに、環境悪化の原因とメカニズムが不明のまま疲弊状態にある有明･八代海の再生修復への貢献を念頭に置き、環境ホルモンなどの環境汚染物質の生物モニタリング科学創成、沿岸域における栄養塩除去などに有効な環境汚染物質のバイオ・レミディエーションの科学技術研究の展開、陸水と海水に跨る沿岸域水環境の計測･解析･評価手法の高度化と応用、微量で多様な環境汚染物質の高精度定量手法の開発と応用などの先導的研究を推進する。また、これらの研究を通じて総合的な研究能力と高度な洞察力を有する創造性豊かな人材を育成するとともに、環境産業創成に貢献する

ミズ カンキョウ オセン ブッシツ ノ ドウタイ ヒョウカ ケンキュウ キョテン ノ コウチク

本拠点形成では、地球規模での省資源･持続･循環社会の実現において中核となる環境科学の分野で画期的な貢献をするために、熊本大学の立地条件に配慮し、緊急性の高い水環境汚染物質の動態評価研究拠点の構築を目指す。とくに、環境悪化の原因とメカニズムが不明のまま疲弊状態にある有明･八代海の再生修復への貢献を念頭に置き、環境ホルモンなどの環境汚染物質の生物モニタリング科学創成、沿岸域における栄養塩除去などに有効な環境汚染物質のバイオ・レミディエーションの科学技術研究の展開、陸水と海水に跨る沿岸域水環境の計測･解析･評価手法の高度化と応用、微量で多様な環境汚染物質の高精度定量手法の開発と応用などの先導的研究を推進する。また、これらの研究を通じて総合的な研究能力と高度な洞察力を有する創造性豊かな人材を育成するとともに、環境産業創成に貢献する