Revisión sistemática de la aplicación de las técnicas de Neurofeedback y Biofeedback en los trastornos de ámbito logopédico

En el presente trabajo se revisan las técnicas de Biofeedback y Neurofeedback, basadas en la retroalimentación de la información fisiológica derivada de cualquier función humana. Se expone de una forma teórica sus posibles implicaciones en los trastornos de la comunicación y las posibilidades de aplicación por parte del logopeda en la rehabilitación de diferentes trastornos acotados al Daño Cerebral Adquirido (DCA) después de relacionarlo con el funcionamiento de estas técnicas para entender la manera en que pueden ser utilizadas. Para llevar a cabo este trabajo, se ha realizado una búsqueda bibliográfica de artículos publicados para llevar a cabo un análisis con distintas palabras clave, en distintos buscadores como Google Académico, Scielo, Dialnet o Pubmed. Los criterios de selección fueron artículos publicados en investigaciones tanto nacionales como internacionales y que referencian tratamientos basados en las técnicas de Biofeedback y Neurofeedback para los trastornos del campo de la logopedia escogidos y presentes en el DCA. Por último, se exponen las conclusiones extraídas, que hacen referencia a la eficacia del Biofeedback en las alteraciones de la comunicación y la deglución, así como la todavía existente necesidad de desarrollar e incentivar su conocimiento en la logopedia.Grado en Logopedi

New properties of a bioinspired pyridine benzimidazole compound as a novel differential staining agent for endoplasmic reticulum and Golgi apparatus in fluorescence live cell imaging

Indexación: Scopus.In this study, we explored new properties of the bioinspired pyridine benzimidazole compound B2 (2,4-di-tert-butyl-6-(3H-imidazo[4,5-c]pyridine-2-yl)phenol) regarding its potential use as a differential biomarker. For that, we performed 1D 1HNMR (TOCSY), UV-Vis absorption spectra in different organic solvents, voltammetry profile (including a scan-rate study), and TD-DFT calculations that including NBO analyses, to provide valuable information about B2 structure and luminescence. In our study, we found that the B2 structure is highly stable, where the presence of an intramolecular hydrogen bond (IHB) seems to have a crucial role in the stability of luminescence, and its emission can be assigned as fluorescence. In fact, we found that the relatively large Stokes Shift observed for B2 (around 175 nm) may be attributed to the stability of the B2 geometry and the strength of its IHB. On the other hand, we determined that B2 is biocompatible by cytotoxicity experiments in HeLa cells, an epithelial cell line. Furthermore, in cellular assays we found that B2 could be internalized by passive diffusion in absence of artificial permeabilization at short incubation times (15 min to 30 min). Fluorescence microscopy studies confirmed that B2 accumulates in the endoplasmic reticulum (ER) and Golgi apparatus, two organelles involved in the secretory pathway. Finally, we determined that B2 exhibited no noticeable blinking or bleaching after 1 h of continuous exposure. Thus, B2 provides a biocompatible, rapid, simple, and efficient way to fluorescently label particular organelles, producing similar results to that obtained with other well-established but more complex methods. © 2018 Llancalahuen, Fuentes, Carreño, Zúñiga, Páez-Hernández, Gacitúa, Polanco, Preite, Arratia-Pérez and Otero.https://www.frontiersin.org/articles/10.3389/fchem.2018.00345/ful

Effect of Terminal Groups of Dendrimers in the Complexation with Antisense Oligonucleotides and Cell Uptake

Indexación: Web of Science.Poly(amidoamine) dendrimers are the most recognized class of dendrimer. Amino-terminated (PAMAM-NH2) and hydroxyl-terminated (PAMAM-OH) dendrimers of generation 4 are widely used, since they are commercially available. Both have different properties, mainly based on their different overall charges at physiological pH. Currently, an important function of dendrimers as carriers of short single-stranded DNA has been applied. These molecules, known as antisense oligonucleotides (asODNs), are able to inhibit the expression of a target mRNA. Whereas PAMAM-NH2 dendrimers have shown to be able to transfect plasmid DNA, PAMAM-OH dendrimers have not shown the same successful results. However, little is known about their interaction with shorter and more flexible molecules such as asODNs. Due to several initiatives, the use of these neutral dendrimers as a scaffold to introduce other functional groups has been proposed. Because of its low cytotoxicity, it is relevant to understand the molecular phenomena involving these types of dendrimers. In this work, we studied the behavior of an antisense oligonucleotide in presence of both types of dendrimers using molecular dynamics simulations, in order to elucidate if they are able to form stable complexes. In this manner, we demonstrated at atomic level that PAMAM-NH2, unlike PAMAM-OH, could form a well-compacted complex with asODN, albeit PAMAM-OH can also establish stable interactions with the oligonucleotide. The biological activity of asODN in complex with PAMAM-NH2 dendrimer was also shown. Finally, we revealed that in contact with PAMAM-OH, asODN remains outside the cells as TIRF microscopy results showed, due to its poor interaction with this dendrimer and cell membranes.http://nanoscalereslett.springeropen.com/articles/10.1186/s11671-016-1260-

Intracellular trafficking and cellular uptake mechanism of PHBV nanoparticles for targeted delivery in epithelial cell lines

Indexación: Web of Science; Scopus; Scielo.Background: Nanotechnology is a science that involves imaging, measurement, modeling and a manipulation of matter at the nanometric scale. One application of this technology is drug delivery systems based on nanoparticles obtained from natural or synthetic sources. An example of these systems is synthetized from poly(3-hydroxybutyrate-co-3-hydroxyvalerate), which is a biodegradable, biocompatible and a low production cost polymer. The aim of this work was to investigate the uptake mechanism of PHBV nanoparticles in two different epithelial cell lines (HeLa and SKOV-3). Results: As a first step, we characterized size, shape and surface charge of nanoparticles using dynamic light scattering and transmission electron microscopy. Intracellular incorporation was evaluated through flow cytometry and fluorescence microscopy using intracellular markers. We concluded that cellular uptake mechanism is carried out in a time, concentration and energy dependent way. Our results showed that nanoparticle uptake displays a cell-specific pattern, since we have observed different colocalization in two different cell lines. In HeLa (Cervical cancer cells) this process may occur via classical endocytosis pathway and some internalization via caveolin-dependent was also observed, whereas in SKOV-3 (Ovarian cancer cells) these patterns were not observed. Rearrangement of actin filaments showed differential nanoparticle internalization patterns for HeLa and SKOV-3. Additionally, final fate of nanoparticles was also determined, showing that in both cell lines, nanoparticles ended up in lysosomes but at different times, where they are finally degraded, thereby releasing their contents. Conclusions: Our results, provide novel insight about PHBV nanoparticles internalization suggesting that for develop a proper drug delivery system is critical understand the uptake mechanism.https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-016-0241-

Adipose tissue-derived mesenchymal stem cells as a strategy to improve recovery after stroke

Introduction: Based on the positive results observed in experimental animal models, adipose tissue-derived mesenchymal stem cells (AD-MSCs) constitute a promising therapy for stroke treatment. However, several aspects need to be clarified to identify the optimal conditions for successful clinical translation. Areas covered: This review focuses on AD-MSC treatment for ischemic and hemorrhagic stroke in experimental animal models. In addition, we will explore the optimization of treatment conditions including AD-MSC production, administration routes and therapeutic windows for their appropriate use in patients, and we will provide an update on clinical trials on this therapy. Expert opinion: Compared with other cell types, AD-MSCs have been less investigated in stroke studies. Currently, experimental animal models have shown safety and efficacy with this treatment after stroke. Due to several advantages of AD-MSCs, such as their abundance and accessibility, they can be considered a promising strategy for use in patients. However, many questions are still to be resolved regarding their mechanisms of action, immune system modulation and the effects of AD-MSCs on all components of the brain that may be affected after ischemic and hemorrhagic strokesThis project is supported by research grants PS12/01754 (Spanish Ministry of Science) and INVICTUS (RD12/0014/0006) (Spanish Neurovascular Network), and Research Institute Carlos III, Ministry Science and Innovatio

Propuesta de innovación docente en educación superior a través del uso de las redes sociales

La capacidad de las redes sociales (RRSS) para comunicar y poner en contacto a millones de personas ha provocado que éstas se usen con fines muy distintos. El mundo educativo no puede permanecer ajeno ante fenómenos sociales como este, por lo que su inclusión en la educación formal es fundamental. Aunque se puede vislumbrar el potencial comunicador y educativo de las RRSS, esto está todavía por descubrir y debe ser estudiado más en profundidad. De este modo, la presente propuesta educativa tiene el foco de atención en las RRSS como herramienta innovadora, comunicativa y de enseñanza-aprendizaje en el marco educativo superior

Propuesta de innovación metodológica en la enseñanza superior: flipped classroom, práctica reflexiva y evaluación formativa

Actualmente se ha demostrado que el aprendizaje es fruto de lo que el alumno hace y no tanto de lo que hace el profesor. La creciente incorporación de metodologías activas en la enseñanza pretende desplazar a la pedagogía tradicional, convirtiendo al alumnado en  (verdadero) protagonista activo de su proceso de enseñanza- aprendizaje. El objetivo de este trabajo es presentar una propuesta de innovación metodológica para la enseñanza superior en la que la acción pedagógica del profesor se enfoque como una orientación dentro de un proceso significativo y constructivo donde el trabajo del alumno tome protagonismo. De esta forma se minimizará el actual déficit entre créditos ECTS y horas reales, en consonancia con las pretensiones del sistema acumulado de transferencia de créditos y nos aproximaremos a una adquisición real de competencias y aprendizajes funcionales por parte del alumnado

Aplicación de diferentes métodos de marcado externo a Octopus vulgaris Cuvier, 1797 con referencia especial a las marcas de tipo T y a los discos de Petersen

En este trabajo se presentan datos de persistencia y efectos provocados por una gran variedad de estilos de marcas (loops, marcas de conejos, cinchas, cintas plásticas flexibles, lancetas, discos de Petersen, marcas de tipo T, marcas plásticas circulares, banderillas plásticas rectangulares, soportes de bisutería, pines comerciales y quemaduras con nitrógeno líquido) aplicadas al cefalópodo Octopus vulgaris Cuvier, 1797 sometido a condiciones de cultivo. Tras una primera revisión de los resultados obtenidos con los diferentes sistemas de marcado se concluye que los discos de Petersen y las marcas de tipo T son los más aconsejables. Ambos muestran una retención adecuada después de un mes (90 y 80 % respectivamente), una persistencia máxima de tres meses, un coste similar y ambos son detectados externamente con facilidad. Sin embargo, los discos de Petersen producen heridas considerables y requieren un proceso de inserción más laborioso, por lo que, finalmente, el sistema considerado más adecuado fue la inserción de marcas de tipo T en el brazo III izquierdoThis paper presents a study on the persistence and effects caused by a wide variety of types of tag (loops, rabbit tags, girths, flexible plastic bands, small darts, Petersen disks, T-bar anchor tags, circular plastic tags, rectangular plastic flags, jewellery pins, commercial badges, and burning with liquid nitrogen) applied to the cephalopod Octopus vulgaris Cuvier, 1797, under culture conditions. Preliminary analysis of the results obtained with the different tagging systems indicates that Petersen disks and T-bar anchor tags are the most suitable types. Both provide adequate retention after 1 month (90 % and 80 %, respectively), a maximum persistence of three months, a similar cost, and they are easily recognised externally. However, Petersen disks can produce serious injuries, and require a more laborious insertion process. Therefore, in the final analysis, the insertion of T-bar anchor tags in left arm III can be considered the most appropriate systemThis work has been carried out with a grant from the Inter-ministerial Commission for Science and Technology and the Instituto Español de Oceanografía, as part of the CYTMAR 97-0323 projec

Search on speech from spoken queries: the Multi-domain International ALBAYZIN 2018 Query-by-Example Spoken Term Detection Evaluation

[Abstract] The huge amount of information stored in audio and video repositories makes search on speech (SoS) a priority area nowadays. Within SoS, Query-by-Example Spoken Term Detection (QbE STD) aims to retrieve data from a speech repository given a spoken query. Research on this area is continuously fostered with the organization of QbE STD evaluations. This paper presents a multi-domain internationally open evaluation for QbE STD in Spanish. The evaluation aims at retrieving the speech files that contain the queries, providing their start and end times, and a score that reflects the confidence given to the detection. Three different Spanish speech databases that encompass different domains have been employed in the evaluation: MAVIR database, which comprises a set of talks from workshops; RTVE database, which includes broadcast television (TV) shows; and COREMAH database, which contains 2-people spontaneous speech conversations about different topics. The evaluation has been designed carefully so that several analyses of the main results can be carried out. We present the evaluation itself, the three databases, the evaluation metrics, the systems submitted to the evaluation, the results, and the detailed post-evaluation analyses based on some query properties (within-vocabulary/out-of-vocabulary queries, single-word/multi-word queries, and native/foreign queries). Fusion results of the primary systems submitted to the evaluation are also presented. Three different teams took part in the evaluation, and ten different systems were submitted. The results suggest that the QbE STD task is still in progress, and the performance of these systems is highly sensitive to changes in the data domain. Nevertheless, QbE STD strategies are able to outperform text-based STD in unseen data domains.Centro singular de investigación de Galicia; ED431G/04Universidad del País Vasco; GIU16/68Ministerio de Economía y Competitividad; TEC2015-68172-C2-1-PMinisterio de Ciencia, Innovación y Competitividad; RTI2018-098091-B-I00Xunta de Galicia; ED431G/0

ALBAYZIN 2018 spoken term detection evaluation: a multi-domain international evaluation in Spanish

[Abstract] Search on speech (SoS) is a challenging area due to the huge amount of information stored in audio and video repositories. Spoken term detection (STD) is an SoS-related task aiming to retrieve data from a speech repository given a textual representation of a search term (which can include one or more words). This paper presents a multi-domain internationally open evaluation for STD in Spanish. The evaluation has been designed carefully so that several analyses of the main results can be carried out. The evaluation task aims at retrieving the speech files that contain the terms, providing their start and end times, and a score that reflects the confidence given to the detection. Three different Spanish speech databases that encompass different domains have been employed in the evaluation: the MAVIR database, which comprises a set of talks from workshops; the RTVE database, which includes broadcast news programs; and the COREMAH database, which contains 2-people spontaneous speech conversations about different topics. We present the evaluation itself, the three databases, the evaluation metric, the systems submitted to the evaluation, the results, and detailed post-evaluation analyses based on some term properties (within-vocabulary/out-of-vocabulary terms, single-word/multi-word terms, and native/foreign terms). Fusion results of the primary systems submitted to the evaluation are also presented. Three different research groups took part in the evaluation, and 11 different systems were submitted. The obtained results suggest that the STD task is still in progress and performance is highly sensitive to changes in the data domain.Ministerio de Economía y Competitividad; TIN2015-64282-R,Ministerio de Economía y Competitividad; RTI2018-093336-B-C22Ministerio de Economía y Competitividad; TEC2015-65345-PXunta de Galicia; ED431B 2016/035Xunta de Galicia; GPC ED431B 2019/003Xunta de Galicia; GRC 2014/024Xunta de Galicia; ED431G/01Xunta de Galicia; ED431G/04Agrupación estratéxica consolidada; GIU16/68Ministerio de Economía y Competitividad; TEC2015-68172-C2-1-