On boosting kernel regression

In this paper we propose a simple multistep regression smoother which is constructed in an iterative manner, by learning the Nadaraya-Watson estimator with L-2 boosting. We find, in both theoretical analysis and simulation experiments, that the bias converges exponentially fast. and the variance diverges exponentially slow. The first boosting step is analysed in more detail, giving asymptotic expressions as functions of the smoothing parameter, and relationships with previous work are explored. Practical performance is illustrated by both simulated and real data

In pursuit of rigour and accountability in participatory design

The field of Participatory Design (PD) has greatly diversified and we see a broad spectrum of approaches and methodologies emerging. However, to foster its role in designing future interactive technologies, a discussion about accountability and rigour across this spectrum is needed. Rejecting the traditional, positivistic framework, we take inspiration from related fields such as Design Research and Action Research to develop interpretations of these concepts that are rooted in PD׳s own belief system. We argue that unlike in other fields, accountability and rigour are nuanced concepts that are delivered through debate, critique and reflection. A key prerequisite for having such debates is the availability of a language that allows designers, researchers and practitioners to construct solid arguments about the appropriateness of their stances, choices and judgements. To this end, we propose a “tool-to-think-with” that provides such a language by guiding designers, researchers and practitioners through a process of systematic reflection and critical analysis. The tool proposes four lenses to critically reflect on the nature of a PD effort: epistemology, values, stakeholders and outcomes. In a subsequent step, the coherence between the revealed features is analysed and shows whether they pull the project in the same direction or work against each other. Regardless of the flavour of PD, we argue that this coherence of features indicates the level of internal rigour of PD work and that the process of reflection and analysis provides the language to argue for it. We envision our tool to be useful at all stages of PD work: in the planning phase, as part of a reflective practice during the work, and as a means to construct knowledge and advance the field after the fact. We ground our theoretical discussions in a specific PD experience, the ECHOES project, to motivate the tool and to illustrate its workings

A physical model of quantum cascade lasers: Application to GaAs, GaN and SiGe devices

The philosophy behind this work has been to build a predictive bottom up physical model of quantum cascade lasers (QCLs) for use as a design tool, to interpret experimental results and hence improve understanding of the physical processes occurring inside working devices and as a simulator for developing new material systems. The standard model uses the envelope function and effective mass approximations to solve two complete periods of the QCL under an applied bias. Other models, such as k·p and empirical pseudopotential, have been employed in p-type systems where the more complex band structure requires it. The resulting wave functions are then used to evaluate all relevant carrier-phonon, carrier-carrier and alloy scattering rates from each quantised state to all others within the same and the neighbouring period. This information is then used to construct a rate equation for the equilibrium carrier density in each subband and this set of coupled rate equations are solved self-consistently to obtain the carrier density in each eigenstate. The latter is a fundamental description of the device and can be used to calculate the current density and gain as a function of the applied bias and temperature, which in turn yields the threshold current and expected temperature dependence of the device characteristics. A recent extension which includes a further iteration of an energy balance equation also yields the average electron (or hole) temperature over the subbands. This paper will review the method and describe its application to mid-infrared and terahertz, GaAs, GaN, SiGe cascade laser designs

Regional and global changes in TCR [alpha][beta] T cell repertoires in the gut are dependent upon the complexity of the enteric microflora

The repertoire of gut associated T cells is shaped by exposure to microbes, including the natural enteric microflora. Previous studies compared the repertoire of gut associated T cell populations in germ free (GF) and conventional mammals often focussing on intra-epithelial lymphocyte compartments. Using GF, conventional and monocolonised (gnotobiotic) chickens and chicken TCRbeta-repertoire analysis techniques, we determined the influence of microbial status on global and regional enteric TCRbeta repertoires. The gut of conventionally reared chickens exhibited non-Gaussian distributions of CDR3-lengths with some shared over-represented peaks in neighbouring gut segments. Sequence analysis revealed local clonal over-representation. Germ-free chickens exhibited a polyclonal, non-selected population of T cells in the spleen and in the gut. In contrast, gnotobiotic chickens exhibited a biased repertoire with shared clones evident throughout the gut. These data indicate the dramatic influence of enteric microflora complexity on the profile of TCRbeta repertoire in the gut at local and global levels

The progamic phase of an early-divergent angiosperm, Annona cherimola (Annonaceae)

33 Pag. The definitive version, with the figures, is available at: http://aob.oxfordjournals.org/Background and Aims: Recent studies of reproductive biology in ancient angiosperm lineages are beginning to shed light on the early evolution of flowering plants, but comparative studies are restricted by fragmented and meagre species representation in these angiosperm clades. In the present study, the progamic phase, from pollination to fertilization, is characterized in Annona cherimola, which is a member of the Annonaceae, the largest extant family among early-divergent angiosperms. Beside interest due to its phylogenetic position, this species is also an ancient crop with a clear niche for expansion in subtropical climates. Methods: The kinetics of the reproductive process was established following controlled pollinations and sequential fixation. Gynoecium anatomy, pollen tube pathway, embryo sac and early post-fertilization events were characterized histochemically. Key Results: A plesiomorphic gynoecium with a semi-open carpel shows a continuous secretory papillar surface along the carpel margins, which run from the stigma down to the obturator in the ovary. The pollen grains germinate in the stigma and compete in the stigma-style interface to reach the narrow secretory area that lines the margins of the semi-open stylar canal and is able to host just one to three pollen tubes. The embryo sac has eight nuclei and is well provisioned with large starch grains that are used during early cellular endosperm development. Conclusions: A plesiomorphic simple gynoecium hosts a simple pollen–pistil interaction, based on a support–control system of pollen tube growth. Support is provided through basipetal secretory activity in the cells that line the pollen tube pathway. Spatial constraints, favouring pollen tube competition, are mediated by a dramatic reduction in the secretory surface available for pollen tube growth at the stigma–style interface. This extramural pollen tube competition contrasts with the intrastylar competition predominant in more recently derived lineages of angiosperms.This work was supported by the Spanish Ministry of Education (Project Grants AGL2004-02290/AGR, AGL2006-13529 and AGL2007-60130/AGR), GIC-Aragón 43, Junta de Andalucía (AGR2742), and the European Union under the INCO-DEV program (Contract 015100). J.L. was supported by a grant from Junta de Andalucía.Peer reviewe

Motif effects in Affymetrix GeneChips seriously affect probe intensities

An Affymetrix GeneChip consists of an array of hundreds of thousands of probes (each a sequence of 25 bases) with the probe values being used to infer the extent to which genes are expressed in the biological material under investigation. In this article, we demonstrate that these probe values are also strongly influenced by their precise base sequence. We use data from >28 000 CEL files relating to 10 different Affymetrix GeneChip platforms and involving nearly 1000 experiments. Our results confirm known effects (those due to the T7-primer and the formation of G-quadruplexes) but reveal other effects. We show that there can be huge variations from one experiment to another, and that there may also be sizeable disparities between batches within an experiment and between CEL files within a batch. © 2012 The Author(s)

Non-monotonic field-dependence of the ZFC magnetization peak in some systems of magnetic nanoparticles

We have performed magnetic measurements on a diluted system of gamma-Fe2O3 nanoparticles (~7nm), and on a ferritin sample. In both cases, the ZFC-peak presents a non-monotonic field dependence, as has already been reported in some experiments,and discussed as a possible evidence of resonant tunneling. Within simple assumptions, we derive expressions for the magnetization obtained in the usual ZFC, FC, TRM procedures. We point out that the ZFC-peak position is extremely sensitive to the width of the particle size distribution, and give some numerical estimates of this effect. We propose to combine the FC magnetization with a modified TRM measurement, a procedure which allows a more direct access to the barrier distribution in a field. The typical barrier values which are obtained with this method show a monotonic decrease for increasing fields, as expected from the simple effect of anisotropy barrier lowering, in contrast with the ZFC results. From our measurements on gamma-Fe2O3 particles, we show that the width of the effective barrier distribution is slightly increasing with the field, an effect which is sufficient for causing the observed initial increase of the ZFC-peak temperatures.Comment: LaTeX file 19 pages, 9 postscript figures. To appear in Phys. Rev. B (tentative schedule: Dec.97

A Beaming-Independent Estimate of the Energy Distribution of Long Gamma-Ray Bursts: Initial Results and Future Prospects

We present single-epoch radio afterglow observations of 24 long-duration gamma-ray burst (GRB) on a timescale of >100 d after the burst. These observations trace the afterglow evolution when the blastwave has decelerated to mildly- or non-relativistic velocities and has roughly isotropized. We infer beaming-independent kinetic energies using the Sedov-Taylor self-similar solution, and find a median value for the sample of detected bursts of about 7x10^51 erg, with a 90% confidence range of 1.1x10^50-3.3x10^53 erg. Both the median and 90% confidence range are somewhat larger than the results of multi-wavelength, multi-epoch afterglow modeling (including large beaming corrections), and the distribution of beaming-corrected gamma-ray energies. This is due to bursts in our sample with only a single-frequency observation for which we can only determine an upper bound on the peak of the synchrotron spectrum. This limitation leads to a wider range of allowed energies than for bursts with a well-measured spectral peak. Our study indicates that single-epoch centimeter-band observations covering the spectral peak on a timescale of ~1 yr can provide a robust estimate of the total kinetic energy distribution with a small investment of telescope time. The substantial increase in bandwidth of the EVLA (up to 8 GHz simultaneously with full coverage at 1-40 GHz) will provide the opportunity to estimate the kinetic energy distribution of GRBs with only a few hours of data per burst.Comment: Submitted to ApJ; 11 pages, 5 figures, 2 table